EA036411B1 - Feed additive of diacetophenonyl selenide and its application for treatment and prevention of selenium deficiency in animals - Google Patents

Abstract

The invention relates to the field of feed production and concerns improvement of animal feed additives containing selenium. A feed additive of diacetophenonyl selenide for animals includes diacetophenonyl selenide, triacetin, expanded perlite in the following ratio, wt.%: diacetophenonyl selenide - 0.315-3.06; triacetin - 35.68-36.69; expanded perlite - the balance. The feed additive is used for the treatment and/or prevention of selenium deficiency in an animal. The invention also relates to a feed composition for animals comprising the feed additive. The feed additive ensures uniform distribution of selenium over the main feed with a large degree of dilution, the full use of selenium by the body of an animal or a bird, and protection of diacetophenonyl selenide from environmental factors.

Description

Technology area

The invention relates to feed production and concerns the improvement of feed additives containing selenium for animals, in particular for farm animals.

State of the art

Selenium is an important trace element, its biological role and importance for the body are multifaceted. Selenium has anti-mutagenic, anti-teratogenic, anti-carcinogenic, radioprotective effects, stimulates anti-toxic protection, normalizes the exchange of nucleic acids, proteins and eicosanoids (prostaglandins, prostacyclins, leukotrienes), regulates the thyroid gland, improves reproduction (reproductive function).

At present, inorganic selenium preparations and feed additives based on sodium selenite and sodium selenate are most widely used to compensate for the selenium deficiency in animals, but these drugs are highly toxic. At the same time, organic compounds of selenium, which are included in the composition of preparations, as well as feed additives (ingredients, premixes), which are added to basic feeds (feed products), exhibit lower toxicity.

Thus, a feed additive and a method for its production are known from the prior art (RU 2577372), consisting of diacetophenonyl selenide, beta-carotene, vitamin E, vitamin C, dissolved in vegetable oil, phospholipids and a filler - wheat bran with the following ratio of components, wt% : diacetophenonyl selenide - 0.001-0.01; beta-carotene - 0.001-0.01; vitamin E - 0.01-0.02; plant phospholipids - 3.0-5.0; vitamin C - 0.01-0.02; vegetable oil - 25.0-27.0, chalk - 3.0-5.0 and wheat bran - the rest.

Known feed additive with antitoxic properties (RU 2637217), consisting of diacetophenonyl selenide, beta-carotene, vitamin E, vitamin C, dissolved in vegetable oil, phospholipids and a filler - wheat bran with the following ratio of components, wt.%: Diacetophenonyl selenide - 0, 05-0.08; beta-carotene - 0.05-0.06; vitamin E - 0.15-0.17; plant phospholipids - 1.5-2.5; vitamin C - 0.15-0.17; vegetable oil - 32.0-33.0; wheat bran - 20.025.0 and the rest is meal.

Also known is a premix for the prevention and treatment of mycotoxicosis in cattle and pigs and a method for its production (RU 2496329), consisting of diacetophenonyl selenide, beta-carotene, vitamin E, vitamin C, dissolved in vegetable oil, phospholipids and a filler - wheat bran in the following ratio components, wt%: diacetophenonyl selenide - 0.001-0.01; beta-carotene - 0.001-0.01; vitamin E - 0.01-0.02; plant phospholipids - 0.1-0.2; vitamin C - 0.010.02; vegetable oil - 29.0-31.0 and wheat bran - the rest.

A distinctive feature of these feed additives is the multicomponent, the use of the organic form of selenium diacetophenonyl selenide, as well as the use of vegetable oil as a solvent for the components. However, these inventions are not aimed at preserving diacetophenonyl selenide, as well as vitamins E and C in the composition of the feed additive, since vegetable oil is both in the process of obtaining the additive (for example, under the action of external factors during dissolution - RU 2637217; high temperatures 90-95 ° C - RU 2577372; 105-110 ° C - RU 2496329), and during storage, when exposed to atmospheric oxygen, it can form peroxide compounds that decompose both diacetophenonyl selenide and vitamins E and C. Also, when combined in solution with diacetophenonyl selenide, vitamin C and beta -carotene are able to restore it, reducing the activity of the selenium compound.

Known feed additive for improving the quality of fur and skins of fur-bearing animals (RU 2600750), containing diacetophenonyl selenide and filler - nanovermiculite. 10-20 g of nanovermiculite contains 1.0 mg of diacetophenonyl selenide. A distinctive feature of this additive is the field of application - for fur animals, the use of sonication to obtain a nanoform of vermiculite, the production of an aqueous suspension of nanovermiculite and diacetophenonyl selenide.

The closest analogue to the claimed feed additive is the feed additive Seleben (RU 2432774), which is a mechanical mixture of diacetophenonyl selenide with bentonite in a ratio of 8-13 g of bentonite per 1-3 mg of diacetophenonyl selenide. A distinctive feature of this invention is the use of bentonite from the Tarn-Varsky deposit. However, this invention is not directed to the protection of diacetophenonyl selenide from the effects of atmospheric oxygen (oxidizing agent), feed components (reducing agents), as well as the possibility of full use or elimination of accumulation in the body due to insolubility in water and significantly limited and slow dissolution of diacetophenonyl selenide in fats and fat-containing mixtures ...

Known feed additive diacetophenonylselenide (DAFS-25 - registration number in Russia - PVR -2-01.12 / 02809) - an organic compound of selenium to replenish selenium in animal feed. The toxicity of selenium in DAFS-25 is significantly lower than in inorganic sodium selenite. The selenium content of the supplement is 25%. The compound very slowly and limitedly dissolves exclusively in fats and is practically insoluble in aqueous media. It is added to compound feed in the amount of 1.6 g per 1 ton. Dilution is 625000 times. The feed additive has established itself as a highly effective source of selenium for animal feed, exhibits an antitoxic effect, suppresses the effect

- 1 036411 mycotoxins. The drug is widely used for animals and birds.

However, there are factors that reduce the effectiveness of this form of supplement:

a high probability of non-uniform distribution in the feed (feed composition) with a high degree of dilution, which requires intermediate dilution;

incomplete use by the body of an animal or bird due to the low rate and low degree of dissolution in the fatty tissues of the animal body, which requires preliminary dissolution;

Diacetophenonyl selenide is susceptible to atmospheric oxygen, reducing agents (eg vitamins A, C) and oxidants (eg calcium iodate) that are invariably present in feed, which lead to a change in the state of the substance, which requires additional protection of the organic selenium compound.

The present invention is aimed at eliminating the listed disadvantages by creating a technological form of the feed additive diacetophenonyl selenide.

Disclosure of invention

The objective of the invention is to create a feed additive for diacetophenonyl selenide for animals; providing high digestibility, stability of the additive to the effects of environmental factors, as well as easily dosed into feed products.

The problem is solved by creating a technological form of a feed additive (ingredient, premix, component) for animals, including: diacetophenonyl selenide, triacetin, expanded perlite in the following mass% ratio: diacetophenonyl selenide - 0.315-3.06; triacetin 35.68-36.69; expanded perlite - the rest.

The problem is also solved by using the feed additive diacetophenonyl selenide for the treatment and / or prevention of selenium deficiency in an animal.

However, in some embodiments, the animal is a mammal, bird, or fish.

The problem is also solved by creating feed compositions for animals, including a feed additive diacetophenonyl selenide.

However, in some embodiments, the animals are mammals or birds.

As a result of the invention, the following technical results are achieved:

a technological form of diacetophenonyl selenide feed additive was created, which ensures uniform distribution over the main feed with a high degree of dilution;

full utilization by the body of an animal or bird is ensured through the use of a form of diacetophenonyl selenide additive dissolved in triacetin on a solid porous carrier, perlite;

protection of diacetophenonyl selenide from environmental factors (air oxygen, as well as from the effects of other feed components - reducing agents, oxidizing agents) is provided.

Terms and Definitions

The following terms and definitions apply in this document unless otherwise specified.

In the description of this invention, the terms include and inclusive are interpreted as meaning includes, but not limited to. These terms are not intended to be construed as consisting only of.

The term technological form means a form of a feed additive that provides ease of dosage, storage, transportation of the finished feed additive, as well as imparting the necessary properties (for example, protection against oxidants).

The term feed additive means an ingredient, a premix, an additive that can be used to obtain feed compositions (for example, compound feed).

The term DAFS-25 means a compound of diacetophenonyl selenide with a mass fraction of selenium in diacetophenol selenide of 25%. DAFS-25 - registration number PVR-2-01.12 / 02809.

Unless otherwise specified, technical and scientific terms in this application have the standard meanings generally accepted in the scientific and technical literature.

Detailed description of the invention

The invention is directed to the development of a feed additive for diacetophenonyl selenide for animals, as well as a method for producing a feed additive of diacetophenonyl selenide and feed compositions based on the feed additive. The feed additive includes an active substance - diacetophenonyl selenide, a solvent - triacetin (1,2,3-triacetoxypropane) and a solid free-flowing carrier (diluent) - perlite.

Diacetophenonylselenide feed additive provides treatment and prevention of selenium deficiency, as well as prevention of mycotoxicosis. The feed additive can be used for the manufacture of feed compositions for animals, in particular for mammals, birds, fish; including farm animals, in particular for farm mammals, birds, fish.

During the development of a feed additive diacetophenonyl selenide, it was unexpectedly discovered that triacetin (1,2,3-triacetoxypropane) can act as a solvent for diacetophenonyl selenide and transfer it to both fat-containing and aquatic environments of animals, while achieving

- 2 036411 maximum availability and fast and complete intake of diacetophenonyl selenide in the organs of animals and poultry, and also excludes accumulation in the animal body due to insolubility in water and significantly limited and slow dissolution of diacetophenonyl selenide in fats and fat-containing mixtures. Triacetin is safe for animals, is a food additive E1518, used in the food industry, in cosmetics and pharmaceuticals. The peculiarity of triacetin is solubility in water and fats, also triacetin does not form peroxide compounds, weakly dissolves oxygen and thus protects diacetophenonyl selenide from decomposition, which differs sharply from known solvents.

Expanded perlite is also included in the additive, due to the distribution of the diacetophenonyl selenide solution over the pore volume of the pearlite, the effect of protection against the decomposition of diacetophenonyl selenide is additionally achieved by eliminating direct contact with reducing agents and oxidizing agents of the components of feed and premixes, the effect of the external environment during storage. Known carriers (diluents) do not possess such properties. A solution of diacetophenonyl selenide in triacetin is displaced from the pores of the perlite by both water and fats (for example, vegetable oil).

Surprisingly, it was found that the protection of diacetophenonyl selenide in the feed additive from environmental factors provides an increase in the shelf life of the diacetophenonyl selenide feed additive.

The method of obtaining a feed additive consists in preliminary converting diacetophenonyl selenide into a soluble state by dissolving it in triacetin, as well as diluting diacetophenonyl selenide by applying a solution of diacetophenonyl selenide in triacetin to a carrier (diluent) perlite.

In this case, diacetophenonyl selenide is converted into a soluble state by dissolving it in triacetin, mixed with a highly volatile solvent from the group of low molecular weight ketones (for example, acetone) or alcohols (for example, isopropyl). The diluent (carrier) expanded perlite is added to the solution with stirring. The mixture is additionally stirred, and the highly volatile solvent is distilled off to a free-flowing state of the residue.

The technological form of the diacetophenonyl selenide feed additive includes, wt%: diacetophenonyl selenide - 0.315-3.06; triacetin 35.68-36.69; expanded perlite - the rest. The ratio of perlite and triacetin can vary depending on the amount of diacetophenonyl selenide, and this ratio corresponds to the state of pearlite, in which it still retains flowability. The content of diacetophenonyl selenide in the feed additive can be varied from 0.315 to 3.06% diacetophenonyl selenide.

The maximum amount of diacetophenonyl selenide (3.06%) is due to the solubility limit in triacetin.

To maintain a constant input of selenium into feed (feed compositions), the amount of the technological form of the feed additive will proportionally change in accordance with the purpose and depending on the main feed diluent. Additional components may also be included in these feed compositions. For example, vitamins, microelements, enzymes in the amounts prescribed by the recipe for a particular species of animals, birds or fish.

It should be understood that these and all examples given in the application materials are not limiting and are given only to illustrate the present invention.

Example 1. A method of obtaining a feed additive diacetophenonyl selenide.

Diacetophenonyl selenide in the form of DAFS-25 is converted into a soluble state by dissolving it in triacetin, mixed with a volatile solvent from the group of low molecular weight ketones (for example, acetone) or alcohols (for example, isopropyl). The diluent (carrier) expanded perlite is added to the solution with stirring. The mixture is additionally stirred, and the highly volatile solvent is distilled off to a free-flowing state of the residue. The following ratios of components are observed: for 20 g of expanded perlite, 10 ml (maximum 11.65 g, depending on the density of the triacetin used) of triacetin, 0.1-1.0 g of DAFS-25 and a volatile solvent to the layer covering the perlite.

Example 2. Confirmation of the protective properties of the feed additive diacetophenonyl selenide.

A sample of the technological form of the feed additive obtained in example 1, with a ratio of 20 g of expanded perlite, 10.0 ml of triacetin and 1.0 g of DAFS-25 was placed for long-term storage in an open colorless container under natural light at room temperature. The content of DAFS-25 was determined periodically by chromatography. The results are presented in the table.

Analysis time, month 0 (storage start) one 3 5 7 nine ten Analysis result, mg / g DAFS-25 31.8 32.8 33.0 31.9 32.2 32.1 32.3

As follows from the above data, DAFS-25 as part of the technological form of the feed additive does not degrade within 10 months of storage with the access of air oxygen and light.

Example 3. Obtaining a feed composition comprising a feed additive with 0.315% diacetophe- 3 036411 nonylselenide.

To obtain the feed composition, the technological form of the feed additive with 0.315% diacetophenonyl selenide is diluted with the components of the feed composition (main feed) to ensure the selenium content in the feed composition is from 0.1 to 0.4 g / t, most preferably 0.3 g / t.

The technological form of the feed additive diacetophenonylselenide, in which 20 g of expanded perlite accounts for 10.0 ml of triacetin and 0.1 g of DAFS-25, for the preparation of a feed composition with this dilution, is introduced into the main feed (for example, in compound feed) in an amount of 254 up to 508 g / t to ensure the amount of selenium in the additive from 0.1 to 0.4 g / t.

Example 4. Obtaining a feed composition comprising a feed additive with 3.06% diacetophenonyl selenide.

To obtain the feed composition, the technological form of the feed additive with 3.06% diacetophenonyl selenide is diluted with the components of the feed composition (main feed) to ensure the selenium content in the feed composition is from 0.1 to 0.4 g / t, most preferably 0.3 g / t ...

The technological form of the feed additive diacetophenonylselenide, in which 20 g of expanded perlite accounts for 10.0 ml of triacetin and 1.0 g of DAFS-25, for the preparation of the feed composition with this dilution is introduced into the main feed (for example, in compound feed) in an amount from 26 to 52 g / t to provide the amount of selenium in the additive from 0.1 to 0.4 g / t.

Example 5. Confirmation of increasing the availability of diacetophenonyl selenide on the example of feeding broilers

Indicators Control, 0.3 g / t Selenium in inorganic form in feed Experiment 1, 0.3 g / t of selenium in the form of DAFS-25 in feed Experiment 2, 0.3 g / t Selenium in the form of a technological form DAFS-25 in feed Live weight on day 14, g% to control 342.2 338.7 -1.02 343.8 +0.47 Live weight on day 21, g% to control 761.5 764.7 +0.42 772.4 +1.01 Live weight on day 35, g% to control 1892.9 1914.6 1.14 1916.5 1.25

The tests were carried out in the poultry house of VNITIP, Sergiev Posad. Each group consisted of 30 broilers. In the experimental groups in the main diet, sodium selenite was replaced by DAFS-25 and the technological form DAFS-25 while maintaining the same level of selenium.

As follows from the above data, the relatively higher availability of diacetophenonyl selenide as part of the technological form of the additive when feeding broilers begins to manifest itself sharply, starting from the earliest age of the bird. For the free, unprotected form of diacetophenonylselenide, the availability in the early stages of poultry growth is significantly lower, and the trend continues until slaughter.

Although the invention has been described with reference to the disclosed embodiments, it should be apparent to those skilled in the art that the specific experiments described in detail are for purposes of illustration only and should not be construed as limiting the scope of the invention in any way. It should be understood that various modifications are possible without departing from the spirit of the invention.

Claims (5)

CLAIM 1. Feed additive for animals, including diacetophenonyl selenide, triacetin, expanded perlite in the following ratio, wt%: diacetophenonyl selenide - 0.315-3.06; triacetin 35.68-36.69; expanded perlite - the rest. 2. The use of a feed additive according to claim 1 for the treatment and / or prevention of selenium deficiency in an animal. 3. Use according to claim 2, wherein the animal is a mammal, bird or fish. 4. A feed composition for animals, comprising a feed additive according to claim 1. 5. The food composition of claim 4, wherein the animal is a mammal, bird, or fish.