WO2019221628A1

WO2019221628A1 - Manufacturable form of the feed additive diacetophenonyl selenide

Info

Publication number: WO2019221628A1
Application number: PCT/RU2018/000307
Authority: WO
Inventors: Сергей Петрович ВОРОНИН; Анатолий Петрович ГУМЕНЮК
Original assignee: Акционерное Общество "Биоамид"
Priority date: 2018-05-15
Filing date: 2018-05-15
Publication date: 2019-11-21

Abstract

The invention relates to the field of feed production and concerns a feed additive for animals that includes diacetophenonyl selenide. The feed additive can be used for the treatment and/or prevention of selenium deficiency in an animal. The invention also relates to an animal feed composition based on the feed additive diacetophenonyl selenide.

Description

Technological form of feed additive diacetophenonylselenide

Technical field

The invention relates to feed production and for the improvement of feed additives containing selenium for animals, in particular for farm animals.

State of the art

Selenium is an important trace element, its biological role and importance for the body are multifaceted. Selenium has anti-mutagenic, anti-teratogenic, anti-carcinogenic, radioprotective effects, stimulates antitoxic defense, normalizes the exchange of nucleic acids, proteins and eicosanoids (prostaglandins, prostacyclins, leukotrienes), regulates the thyroid gland, improves reproduction (reproductive function).

Currently, inorganic preparations of selenium and feed additives based on selenite and sodium selenate are most widely used to compensate for selenium deficiency in animals, however, these preparations are highly toxic. At the same time, organic compounds of selenium exhibit lower toxicity, which are included in the composition of preparations, as well as feed additives (ingredients, premixes), which are added to the main feed (feed products).

Thus, a feed additive and a method for its preparation (RU2577372) are known from the prior art, consisting of diacetophenonyl selenide, beta-carotene, vitamin E, vitamin C dissolved in vegetable oil, phospholipids and filler - wheat bran in the following ratio of components, May. % diacetophenonyl selenide - 0.001-0.01; beta-carotene - 0.001-0.01; Vitamin E - 0.01-0.02; plant phospholipids - 3, 0-5.0; Vitamin C - 0.01-0.02; vegetable oil - 25.0-27.0, chalk - 3.0-5.0, and wheat bran - the rest.

Known feed additive with antitoxic properties (RU 2637217), consisting of diacetophenonyl selenide, beta-carotene, vitamin E, vitamin C, dissolved in vegetable oil, phospholipids and filler - wheat bran in the following ratio, May. % diacetophenonyl selenide 0.05-0.08; beta-carotene - 0.05-0.06; Vitamin E - 0.15-0.17; plant phospholipids - 1, 5-2, 5; vitamin C - 0.15-0.17; vegetable oil - 32.0-33.0; wheat bran - 20.0-25.0 and the rest - meal.

Also known is a premix for the prevention and treatment of mycotoxicosis of cattle and pigs, and a method for its production (RU2496329), consisting of diacetophenonyl selenide, beta-carotene, vitamin E, vitamin C dissolved in vegetable oil, phospholipids and filler - wheat bran in the following ratio of components, May. % diacetophenonyl selenide - 0.001-0.01; beta carotene -

0.001-0.01; Vitamin E - 0.01-0.02; plant phospholipids - 0.1-0.2; Vitamin C - 0.01-

0.02; vegetable oil - 29.0-31, 0 and wheat bran - the rest.

A distinctive feature of the data. feed additives is multicomponent, the use of the organic form of selenium diacetophenonyl selenide, as well as the use of vegetable oil as a solvent component. However, these inventions are not aimed at preserving diacetophenonyl selenide, as well as vitamins E and C, in the composition of the feed additive, since vegetable oil is in the process of producing the additive (for example, under the influence of external factors during dissolution - RU2637217; high temperatures 90-95 ° С - RU2577372; 10б- ll 0 ° С - RU2496329), and during storage under the influence of atmospheric oxygen it can form peroxide compounds that decompose both diacetophenonyl selenide and vitamins E and C. Also, when combined with diacetophenonyl selenide in solution, amine C and beta-carotene are able to recover it, reducing the activity of selenium compounds.

Known feed additive to improve the quality of the fur and skins of fur animals (RU2600750), containing diacetophenonyl selenide and a filler - nanoermiculite. 10-20 g of nanoermuliculite contains 1.0 mg of diacetophenonyl selenide. A distinctive feature of this additive is the field of application - for fur animals, the use of ultrasonic treatment to obtain vermiculite nanoforms, and the preparation of an aqueous suspension of nanovermiculite and diacetophenonyl selenide.

The closest analogue to the claimed feed additive is the Seleben feed additive (RU2432774), which is a mechanical mixture of diacetophenonyl selenide with bentonite in the ratio of 8-13 g of bentonite to 1-3 mg of diacetophenonyl selenide. A distinctive feature of this invention is the use of bentonite Tarn-Var deposits. However, this invention is not aimed at protecting diacetophenonyl selenide from the effects of air oxygen (oxidizing agent), feed components (reducing agents), and also at the possibility of full use or elimination of accumulation in the body due to water insolubility and significantly limited and slow dissolution of diacetophenonyl selenide in fats and fat-containing mixtures .

Known feed additive diacetophenonyl selenide (DAFS-25 - registration number in Russia - TAC -2-01.12 / 02809) - an organic compound of selenium to replenish selenium in animal feed. The toxicity of selenium in DAFS-25 is significantly lower than in inorganic sodium selenite. The content of selenium in the additive is 25%. The compound is very slowly and sparingly soluble exclusively in fats and practically insoluble in aqueous media. It is added to feed in the amount of 1, 6 g per 1 ton. Dilution 625,000 times. The feed supplement has established itself as highly effective source of selenium for animal feed, exhibits an antitoxic effect, inhibits the action of mycotoxins. The drug is widely used for animals and birds.

However, there are factors that reduce the effectiveness of this form of the additive:

- a high probability of a non-uniform distribution over the feed (feed composition) with a large degree of dilution, which requires an intermediate dilution;

- incomplete use by the body of an animal or bird due to the low speed and low degree of dissolution in the fatty tissues of the animal organism, which requires preliminary dissolution;

- diacetophenonyl selenide is exposed to atmospheric oxygen, reducing agents (e.g. vitamins A, C) and oxidizing agents (e.g. calcium iodate) that are invariably present in feeds, which lead to a change in the state of the substance, which requires additional protection of the organic compound of selenium.

The present invention is aimed at eliminating the above disadvantages by creating a technological form of a feed additive of diacetophenonyl selenide.

Disclosure of invention

An object of the present invention is to provide a feed additive for diacetophenonyl selenide for animals; providing high digestibility, stability of the additive to the effects of environmental factors, as well as easily dosed in feed products.

The problem is solved by creating a technological form of a feed additive (ingredient, premix, component) for animals, including: diacetophenonyl selenide, triacetin, expanded perlite in the following mass ratio: diacetophenonyl selenide - 0.315 - 3.06; triacetin -35.68 - 36.69; expanded perlite - rest

The problem is also solved by the use of feed additives diacetophenonylselenide, for the treatment and / or prevention of selenium deficiency in an animal.

However, in some embodiments of the invention, the animal is a mammal, bird or fish.

The problem is also solved by creating feed compositions for animals, including a feed additive of diacetophenonyl selenide.

However, in some embodiments of the invention, the animals are mammals or birds.

As a result of the invention, the following technical results are achieved: - the technological form of the feed additive of diacetophenonyl selenide was created, which ensures uniform distribution over the main feed with a large degree of dilution;

- ensures the full use by the body of an animal or bird through the use of a form of diacetophenonyl selenide additive dissolved in triacetin on a solid porous carrier - perlite;

- diacetophenonyl selenide is protected from environmental factors (atmospheric oxygen, as well as from the effects of other feed components - reducing agents, oxidizing agents).

Terms and Definitions

The following terms and definitions apply throughout this document unless otherwise indicated.

In the description of the present invention, the terms “includes” and “including” are interpreted as meaning “includes, inter alia,”. These terms are not intended to be construed as “consists of only”.

The term "technological form" means the form of a feed additive providing ease of dosage, storage, transportation of the finished feed additive, as well as giving it the necessary properties (for example, protection against oxidizing agents).

The term "feed additive" means an ingredient, premix, an additive that can be used to obtain feed compositions (for example, feed).

The term "DAFS-25" means a compound of diacetophenonyl selenide with a mass fraction of selenium in diacetophenol selenide - 25%. DAFS-25 - Registration number PVR -2-01.12 / 02809.

Unless defined separately, technical and scientific terms in this application have standard meanings generally accepted in the scientific and technical literature.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is directed to the development of a feed additive for diacetophenonyl selenide for animals, as well as a method for producing a feed additive for diacetophenonyl selenide and feed compositions based on a feed additive. The feed additive includes the active substance - diacetophenonyl selenide, the solvent - triacetin (1, 2,3-triacetoxypropane) and a solid granular carrier (diluent) -perlite.

The dietary supplement of diacetophenonyl selenide provides treatment and prevention of selenium deficiency, as well as the prevention of mycotoxicosis. The feed additive can be used for the manufacture of feed compositions for animals, in particular for mammals, birds, fish; including farm animals, in the particular case for farm mammals, birds, fish.

During the development of a feed additive for diacetophenonyl selenide, it was unexpectedly discovered that triacetin (1, 2,3-triacetoxypropane) can act as a solvent diacetophenonyl selenide and transfers it both to fat-containing and aquatic environments of the animal organism, while maximizing the availability and rapid and complete intake of diacetophenonyl selenide in the organs of animals and birds, and also accumulating in the animal body due to water insolubility and significantly limited and slow dissolution of diacetophenonyl selenide in fats and fat-containing mixtures. Triacetin is safe for animals, is a dietary supplement

E1518, is used in the food industry, in cosmetics and medicines. The peculiarity of triacetin is its solubility in water and fats, also triacetin does not form peroxidic compounds, slightly dissolves oxygen and thus protects diacetophenonyl selenide from decomposition, which differs sharply from known solvents.

Expanded perlite is also included in the composition of the additive, due to the distribution of the diacetophenonyl selenide solution in the pore volume of perlite, the protection against diacetophenonyl selenide decomposition is additionally achieved due to the exclusion of direct contact with the reducing agents and oxidizing agents of compound feeds and premixes, and the environmental impact during storage. Known carriers (diluents) do not possess such properties. The solution of diacetophenonyl selenide in triacetin is displaced from the pearlite pores with both water and fats (for example, vegetable oil).

It was unexpectedly found that the protection of diacetophenonyl selenide in the feed additive from environmental factors provides an increase in the shelf life of the feed additive diacetophenonyl selenide.

A method of obtaining a feed additive consists in preliminary converting diacetophenonyl selenide into a soluble state by dissolving it in triacetin, as well as diluting diacetophenonyl selenide by applying a solution of diacetophenonyl selenide in triacetin to a perlite carrier (diluent).

In this case, diacetophenonyl selenide is converted into a soluble state by dissolving it in triacetin, mixed with a volatile solvent from the group of low molecular weight ketones (e.g. acetone) or alcohols (e.g. isopropyl). Expanded perlite diluent (carrier) is added to the solution with stirring. The mixture is further mixed and the volatile solvent is distilled off to a free-flowing state of the residue.

The technological form of the feed additive of diacetophenonyl selenide includes in mass%: diacetophenonyl selenide - 0.315 - 3.06; triacetin - 35.68 - 36.69; swollen perlite - the rest The ratio of perlite and triacetin can vary depending on the amount of diacetophenonyl selenide, and this ratio corresponds to the state of perlite, in which it still maintains flowability. The content of diacetophenonyl selenide in the feed additive can be changed from 0.315 to 3.06% diacetophenonyl selenide. The maximum amount of diacetophenonyl selenide (3.06%) is due to the solubility limit in triacetin.

To maintain the constant input of selenium into the feed (feed composition), the quantity of the technological form of the feed additive will be proportionally changed in accordance with the purpose and depending on the main feed diluent. Additional components may also be included in the composition of these feed compositions. For example, vitamins, trace elements, enzymes in the amounts prescribed by the recipe for a particular type of animal, bird or fish.

It should be understood that these and all examples cited in the application materials are not limiting and are provided merely to illustrate the present invention.

Example 1. A method of obtaining a feed additive diacetophenonylselenide.

Diacetophenonyl selenide in the form of DAFS-25 is converted into a soluble state by dissolving it in triacetin, mixed with a volatile solvent from the group of low molecular weight ketones (e.g. acetone) or alcohols (e.g. isopropyl). Expanded perlite diluent (carrier) is added to the solution with stirring. The mixture is further mixed and the volatile solvent is distilled off to a free-flowing state of the residue. The following ratios of components are observed: per 20 g of expanded perlite, 10 ml (maximum 11, 65 g, depending on the density of the triacetin used), triacetin, 0.1-1.0 g of DAFS-25, and a volatile solvent to the perlite coating layer.

Example 2. Confirmation of the protective properties of the feed additive diacetophenonyl selenide

A sample of the technological form of the feed additive obtained in example 1, with a ratio of 20 g of expanded perlite, 10.0 ml of triacetin and 1.0 g of DAFS-25, was placed for long-term storage in an open colorless container under natural light at room temperature. The content of DAFS-25 was determined periodically by chromatography. The results are presented in table 1.

Table 1.

As follows from the above data, DAFS-25 as part of the technological form of the feed additive is not destroyed during 10 months of storage with the access of oxygen from the air and light. Example 3. Obtaining a feed composition comprising a feed additive with

0.315% diacetophenonyl selenide.

To obtain a feed composition, the technological form of the feed additive is diluted with 0.315% diacetophenonyl selenide components of the feed composition (main feed) to provide a selenium content in the feed composition of 0.1 to 0.4 g / t, most preferably 0.3 g / t.

The technological form of the feed additive of diacetophenonyl selenide, in which 10.0 ml of triacetin and 0.1 g of DAFS-25 account for 20 g of expanded perlite, for the preparation of the feed composition with this dilution, is introduced into the main feed (for example, in compound feeds) in an amount from 254 to 508 g / t to ensure the amount of selenium in the additive is from 0.1 to 0.4 g / t.

Example 4. Obtaining a feed composition comprising a feed additive with 3.06% of diacetophenonyl selenide.

To obtain a feed composition, the technological form of the feed additive is diluted with 3.06% of diacetophenonyl selenide components of the feed composition (main feed) to provide selenium content in the feed composition from 0.1 to 0.4 g / t, most preferably 0.3 g / t .

The technological form of the feed additive of diacetophenonyl selenide, in which 10.0 ml of triacetin and 1.0 g of DAFS-25 account for 20 g of expanded perlite, for the preparation of the feed composition with this dilution, is introduced into the main feed (for example, in compound feed) in an amount from 26 to 52 g / t to ensure the amount of selenium in the additive is from 0.1 to 0.4 g / t.

Example 5. Confirmation of the increased availability of diacetophenonyl selenide by the example of broiler feeding

The tests were carried out in the house VNITIP Sergiev Posad. In each group there were 30 goals of broilers. In the experimental groups in the main diet, sodium selenite was replaced by DAFS-25 and the technological form of DAFS-25 while maintaining the same level of selenium.

As follows from the above data, the relatively higher availability of diacetophenonyl selenide as part of the technological form of the additive when feeding broilers begins to manifest itself sharply, starting from the very early age of the bird. For the free unprotected form of diacetophenonyl selenide, availability in the early stages of poultry growth is significantly lower, and the trend continues until slaughter

Although the invention has been described with reference to the disclosed embodiments, it will be apparent to those skilled in the art that the specific experiments described in detail are for illustrative purposes only and should not be construed as limiting the scope of the invention in any way. It should be understood that various modifications are possible without departing from the gist of the present invention.

Claims

Claim

1. A feed additive of diacetophenonyl selenide for animals, including: diacetophenonyl selenide, triacetin, expanded perlite in the following ratio, mass%:

diacetophenonyl selenide 0.315-3.06;

triacetin -35.68 - 36.69;

expanded perlite - the rest.

2. The use of a feed additive according to claim 1, for the treatment and / or prevention of selenium deficiency in an animal.

3. The use of claim 2, wherein the animal is a mammal, bird, or fish.

4. A feed composition for animals, comprising a feed additive according to claim 1.

5. The feed composition according to claim 5, where the animals are mammals, birds or fish.