DK3765616T3 - Nye enzymer og systemer målrettet crispr dna og rna - Google Patents

Nye enzymer og systemer målrettet crispr dna og rna Download PDF

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Publication number
DK3765616T3
DK3765616T3 DK19717640.7T DK19717640T DK3765616T3 DK 3765616 T3 DK3765616 T3 DK 3765616T3 DK 19717640 T DK19717640 T DK 19717640T DK 3765616 T3 DK3765616 T3 DK 3765616T3
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Denmark
Prior art keywords
rna
new enzymes
targeting crispr
crispr dna
systems targeting
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Application number
DK19717640.7T
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English (en)
Inventor
Elise Keston Smith
David A Scott
David R Cheng
Winston X Yan
Pratyusha Hunnewell
Jason Carte
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Arbor Biotechnologies Inc
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Publication of DK3765616T3 publication Critical patent/DK3765616T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
DK19717640.7T 2018-03-14 2019-03-14 Nye enzymer og systemer målrettet crispr dna og rna DK3765616T3 (da)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US201862642919P 2018-03-14 2018-03-14
US201862666397P 2018-05-03 2018-05-03
US201862672489P 2018-05-16 2018-05-16
US201862699498P 2018-07-17 2018-07-17
US201862729393P 2018-09-10 2018-09-10
US201862740867P 2018-10-03 2018-10-03
US201862746539P 2018-10-16 2018-10-16
US201862772043P 2018-11-27 2018-11-27
US201862775874P 2018-12-05 2018-12-05
PCT/US2019/022376 WO2019178428A1 (en) 2018-03-14 2019-03-14 Novel crispr dna and rna targeting enzymes and systems

Publications (1)

Publication Number Publication Date
DK3765616T3 true DK3765616T3 (da) 2023-08-21

Family

ID=66175481

Family Applications (1)

Application Number Title Priority Date Filing Date
DK19717640.7T DK3765616T3 (da) 2018-03-14 2019-03-14 Nye enzymer og systemer målrettet crispr dna og rna

Country Status (8)

Country Link
US (1) US20230242891A1 (da)
EP (2) EP3765616B1 (da)
AU (1) AU2019236211A1 (da)
DK (1) DK3765616T3 (da)
ES (1) ES2953541T3 (da)
FI (1) FI3765616T3 (da)
PT (1) PT3765616T (da)
WO (1) WO2019178428A1 (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018201086A1 (en) 2017-04-28 2018-11-01 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
EP3635104A1 (en) 2017-06-09 2020-04-15 Editas Medicine, Inc. Engineered cas9 nucleases
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
HRP20230828T1 (hr) 2018-03-14 2023-11-10 Arbor Biotechnologies, Inc. Novi sustavi i enzimi koji ciljaju na crispr dna
US20210198664A1 (en) 2018-05-16 2021-07-01 Arbor Biotechnologies, Inc. Novel crispr-associated systems and components
US20220073890A1 (en) 2018-12-14 2022-03-10 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
CN110596384B (zh) * 2019-10-12 2022-08-12 南京黎明生物制品有限公司 基于Cas蛋白及gRNA复合物制备的人乳头瘤病毒6型和11型免疫检测试剂盒
CN112986551B (zh) * 2019-12-11 2022-09-27 北京聚树生物科技有限公司 一种检测混合体系内目标分子浓度的方法及试剂盒
CA3168830A1 (en) * 2020-01-27 2021-08-05 Sherlock Biosciences, Inc. Improved detection assays
CN112877410B (zh) * 2020-12-30 2022-09-13 东北大学 一种优化的基于crispr介导的核酸检测系统及其检测方法
CN112725343A (zh) * 2021-01-22 2021-04-30 南京工业大学 联合金纳米探针和CRISPR-Cas的蛋白标志物检测试剂盒及检测方法
CA3222023A1 (en) 2021-06-01 2022-12-08 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
CN113552103B (zh) * 2021-07-20 2022-12-30 济南大学 一种基于CRISPR-Cas系统的检测外泌体的荧光生物传感器
TW202321454A (zh) * 2021-07-26 2023-06-01 美商夏洛克生物科學公司 經改良之ｃｒｉｓｐｒ－ｃａｓ技術
CN113583876A (zh) * 2021-08-02 2021-11-02 中国农业科学院上海兽医研究所(中国动物卫生与流行病学中心上海分中心) EYFP敲入到Et.Actin基因的鸡柔嫩艾美尔球虫虫株的制备

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
US5587308A (en) 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
US5593972A (en) 1993-01-26 1997-01-14 The Wistar Institute Genetic immunization
AU2005274948B2 (en) 2004-07-16 2011-09-22 Genvec, Inc. Vaccines against aids comprising CMV/R-nucleic acid constructs
EP3252160B1 (en) 2012-12-12 2020-10-28 The Broad Institute, Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
MX2015007550A (es) 2012-12-12 2017-02-02 Broad Inst Inc Suministro, modificación y optimización de sistemas, métodos y composiciones para la manipulación de secuencias y aplicaciones terapéuticas.
ES2857751T3 (es) 2013-10-11 2021-09-29 Massachusetts Eye & Ear Infirmary Métodos para predecir secuencias de virus ancestrales y usos de los mismos
CN106459995B (zh) 2013-11-07 2020-02-21 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
EP3230452A1 (en) 2014-12-12 2017-10-18 The Broad Institute Inc. Dead guides for crispr transcription factors
WO2016106236A1 (en) * 2014-12-23 2016-06-30 The Broad Institute Inc. Rna-targeting system
FI3430134T3 (fi) 2015-06-18 2023-01-13 Uusia CRISPR-entsyymejä ja järjestelmiä
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CN109153980B (zh) 2015-10-22 2023-04-14 布罗德研究所有限公司 Vi-b型crispr酶和系统
WO2017091630A1 (en) * 2015-11-23 2017-06-01 The Regents Of The University Of California Tracking and manipulating cellular rna via nuclear delivery of crispr/cas9
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
US11788083B2 (en) 2016-06-17 2023-10-17 The Broad Institute, Inc. Type VI CRISPR orthologs and systems

Also Published As

Publication number Publication date
PT3765616T (pt) 2023-08-28
WO2019178428A1 (en) 2019-09-19
EP4253551A2 (en) 2023-10-04
FI3765616T3 (fi) 2023-08-29
EP3765616A1 (en) 2021-01-20
AU2019236211A1 (en) 2020-09-17
US20230242891A1 (en) 2023-08-03
ES2953541T3 (es) 2023-11-14
EP3765616B1 (en) 2023-07-19
EP4253551A3 (en) 2024-01-24

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