DK3655422T3 - Promotor til heterolog ekspression - Google Patents

Promotor til heterolog ekspression Download PDF

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Publication number
DK3655422T3
DK3655422T3 DK18740570.9T DK18740570T DK3655422T3 DK 3655422 T3 DK3655422 T3 DK 3655422T3 DK 18740570 T DK18740570 T DK 18740570T DK 3655422 T3 DK3655422 T3 DK 3655422T3
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DK
Denmark
Prior art keywords
promoter
heterologous expression
heterologous
expression
Prior art date
Application number
DK18740570.9T
Other languages
English (en)
Other versions
DK3655422T5 (da
Inventor
Max Fabian Felle
Bogdan Tokovenko
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Application granted granted Critical
Publication of DK3655422T3 publication Critical patent/DK3655422T3/da
Publication of DK3655422T5 publication Critical patent/DK3655422T5/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/34—Vector systems having a special element relevant for transcription being a transcription initiation element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DK18740570.9T 2017-07-21 2018-07-11 Promotor til heterolog ekspression DK3655422T5 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17182615 2017-07-21
EP17209978 2017-12-22
PCT/EP2018/068847 WO2019016052A1 (en) 2017-07-21 2018-07-11 HETEROLOGOUS EXPRESSION PROMOTER

Publications (2)

Publication Number Publication Date
DK3655422T3 true DK3655422T3 (da) 2023-06-19
DK3655422T5 DK3655422T5 (da) 2024-08-26

Family

ID=62909527

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18740570.9T DK3655422T5 (da) 2017-07-21 2018-07-11 Promotor til heterolog ekspression

Country Status (8)

Country Link
US (1) US12065652B2 (da)
EP (1) EP3655422B1 (da)
CN (1) CN110945013A (da)
DK (1) DK3655422T5 (da)
ES (1) ES2943019T3 (da)
FI (1) FI3655422T3 (da)
PL (1) PL3655422T3 (da)
WO (1) WO2019016052A1 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3655528A1 (en) 2017-07-21 2020-05-27 Basf Se Method of transforming bacterial cells
CN116254268B (zh) * 2023-05-11 2023-08-15 北京蓝晶微生物科技有限公司 一种启动子库及其在不同细菌中的应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4005025A1 (de) 1989-04-28 1990-10-31 Henkel Kgaa Verbessertes transformationsverfahren
EP0493398B1 (en) 1989-08-25 1999-12-08 Henkel Research Corporation Alkaline proteolytic enzyme and method of production
DK639689D0 (da) 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler
US5958728A (en) 1996-11-18 1999-09-28 Novo Nordiskbiotech, Inc. Methods for producing polypeptides in mutants of bacillus cells
US7247450B2 (en) * 2002-02-08 2007-07-24 Genencor International, Inc. Secretion, transcription and sporulation genes in Bacillus clausii
US7026149B2 (en) 2003-02-28 2006-04-11 Ajinomoto Co., Inc. Polynucleotides encoding polypeptides involved in the stress response to environmental changes in Methylophilus methylotrophus
DE10309557A1 (de) 2003-03-04 2004-09-23 Henkel Kgaa Ein Translokationsenzym als Selektionsmarker
CN105992817B (zh) * 2014-02-07 2020-09-11 帝斯曼知识产权资产管理有限公司 改良的芽孢杆菌宿主

Also Published As

Publication number Publication date
DK3655422T5 (da) 2024-08-26
CN110945013A (zh) 2020-03-31
PL3655422T3 (pl) 2023-07-24
EP3655422B1 (en) 2023-03-29
WO2019016052A1 (en) 2019-01-24
ES2943019T3 (es) 2023-06-08
US12065652B2 (en) 2024-08-20
FI3655422T3 (fi) 2023-06-13
EP3655422A1 (en) 2020-05-27
US20200181627A1 (en) 2020-06-11

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