DK3527068T3 - Fremgangsmåder og sammensætninger til forøgelse af effektivitet af målrettet genmodifikation under anvendelse af oligonukleotid-medieret genreparation - Google Patents

Fremgangsmåder og sammensætninger til forøgelse af effektivitet af målrettet genmodifikation under anvendelse af oligonukleotid-medieret genreparation Download PDF

Info

Publication number
DK3527068T3
DK3527068T3 DK19151652.5T DK19151652T DK3527068T3 DK 3527068 T3 DK3527068 T3 DK 3527068T3 DK 19151652 T DK19151652 T DK 19151652T DK 3527068 T3 DK3527068 T3 DK 3527068T3
Authority
DK
Denmark
Prior art keywords
genomification
oligonucleotide
targeted
compositions
increasing
Prior art date
Application number
DK19151652.5T
Other languages
Danish (da)
English (en)
Inventor
Peter R Beetham
Gregory F W Gocal
Noel Joy Sauer
James Pearce
Rosa Segami
Jerry Mozoruk
Christian Schopke
Original Assignee
Cibus Us Llc
Cibus Europe Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cibus Us Llc, Cibus Europe Bv filed Critical Cibus Us Llc
Application granted granted Critical
Publication of DK3527068T3 publication Critical patent/DK3527068T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK19151652.5T 2013-03-15 2014-03-14 Fremgangsmåder og sammensætninger til forøgelse af effektivitet af målrettet genmodifikation under anvendelse af oligonukleotid-medieret genreparation DK3527068T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361801333P 2013-03-15 2013-03-15
EP14764596.4A EP2966980A4 (en) 2013-03-15 2014-03-14 METHODS AND COMPOSITIONS FOR INCREASING THE EFFICACY OF THE ENHANCED EFFICACY OF TARGETED GENE MODIFICATION USING OLIGONUCLEOTIDE MEDIATED GENE REPAIR

Publications (1)

Publication Number Publication Date
DK3527068T3 true DK3527068T3 (da) 2022-06-27

Family

ID=51537837

Family Applications (1)

Application Number Title Priority Date Filing Date
DK19151652.5T DK3527068T3 (da) 2013-03-15 2014-03-14 Fremgangsmåder og sammensætninger til forøgelse af effektivitet af målrettet genmodifikation under anvendelse af oligonukleotid-medieret genreparation

Country Status (25)

Country Link
US (2) US10287594B2 (ja)
EP (3) EP3527068B1 (ja)
JP (4) JP6591394B2 (ja)
KR (5) KR102647792B1 (ja)
CN (2) CN105431039B (ja)
AU (3) AU2014233519B2 (ja)
BR (1) BR112015022829B1 (ja)
CA (2) CA2905391C (ja)
CL (1) CL2015002648A1 (ja)
DK (1) DK3527068T3 (ja)
EA (1) EA201591445A1 (ja)
ES (1) ES2921207T3 (ja)
HK (1) HK1222507A1 (ja)
HR (1) HRP20220803T1 (ja)
HU (1) HUE059269T2 (ja)
IL (4) IL290273B2 (ja)
LT (1) LT3527068T (ja)
MY (1) MY183741A (ja)
PL (1) PL3527068T3 (ja)
PT (1) PT3527068T (ja)
RS (1) RS63427B1 (ja)
SI (1) SI3527068T1 (ja)
UA (1) UA120033C2 (ja)
WO (1) WO2014144951A1 (ja)
ZA (2) ZA201507555B (ja)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3613852A3 (en) 2011-07-22 2020-04-22 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
EA201591445A1 (ru) 2013-03-15 2016-03-31 Сибас Юс Ллс Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов
US20230313211A1 (en) * 2013-03-15 2023-10-05 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
CA3157565A1 (en) * 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
KR102450868B1 (ko) * 2014-03-14 2022-10-06 시버스 유에스 엘엘씨 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물
CA2956224A1 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
EP4372091A3 (en) * 2014-12-12 2024-07-31 Tod M. Woolf Compositions and methods for editing nucleic acids in cells utilizing oligonucleotides
JP2016202038A (ja) * 2015-04-17 2016-12-08 国立大学法人北陸先端科学技術大学院大学 塩基置換手段をスクリーニングする方法
CN107709562A (zh) * 2015-05-15 2018-02-16 先锋国际良种公司 指导rna/cas内切核酸酶系统
CN108368502B (zh) 2015-06-03 2022-03-18 内布拉斯加大学董事委员会 使用单链dna的dna编辑
AU2016342380B2 (en) 2015-10-23 2022-04-07 President And Fellows Of Harvard College Nucleobase editors and uses thereof
US20190300872A1 (en) * 2016-05-06 2019-10-03 Tod M. Woolf Improved Methods of Genome Editing with and without Programmable Nucleases
GB2568182A (en) 2016-08-03 2019-05-08 Harvard College Adenosine nucleobase editors and uses thereof
JP7201153B2 (ja) 2016-08-09 2023-01-10 プレジデント アンド フェローズ オブ ハーバード カレッジ プログラム可能ｃａｓ９－リコンビナーゼ融合タンパク質およびその使用
EP4056701A1 (en) * 2016-08-12 2022-09-14 Nexuspiral Inc. Genome editing method
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
BR112019012155A2 (pt) 2016-12-14 2019-11-12 Stichting Technische Wetenschappen uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína
WO2018109101A1 (en) * 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
SG11202109679VA (en) 2019-03-19 2021-10-28 Broad Inst Inc Methods and compositions for editing nucleotide sequences
MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.
CN112042542A (zh) * 2020-09-15 2020-12-08 上海市农业科学院 一种茄子高效再生体系的建立方法
JPWO2022249775A1 (ja) * 2021-05-27 2022-12-01
WO2024160989A1 (en) 2023-02-03 2024-08-08 Syngenta Crop Protection Ag Herbicide resistant plants

Family Cites Families (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5024944A (en) 1986-08-04 1991-06-18 Lubrizol Genetics, Inc. Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5008200A (en) 1988-05-08 1991-04-16 United Agriseeds, Inc. Propagating multiple whole fertile plants from immature leguminous
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5219746A (en) 1990-07-19 1993-06-15 Chris Brinegar Ice-mediated introduction of substances into biological material
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
US5641625A (en) 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
DE4216134A1 (de) 1991-06-20 1992-12-24 Europ Lab Molekularbiolog Synthetische katalytische oligonukleotidstrukturen
WO1993004177A1 (en) 1991-08-27 1993-03-04 Agricultural Genetics Company Limited Proteins with insecticidal properties against homopteran insects and their use in plant protection
TW261517B (ja) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5593874A (en) 1992-03-19 1997-01-14 Monsanto Company Enhanced expression in plants
IT230274Y1 (it) 1993-06-11 1999-06-02 Silc Spa Pannolone assorbente sagomato per incontinenza
US5962426A (en) 1993-06-25 1999-10-05 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis
WO1995015972A1 (en) * 1993-12-09 1995-06-15 Thomas Jefferson University Compounds and methods for site-directed mutations in eukaryotic cells
EP0679657B1 (de) 1994-04-27 2003-07-09 Novartis AG Nukleoside und Oligonukleotide mit 2'-Ethergruppen
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5780296A (en) 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
US5760012A (en) 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations
US5731181A (en) 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
US5888983A (en) 1996-05-01 1999-03-30 Thomas Jefferson University Method and oligonucleobase compounds for curing diseases caused by mutations
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US6524613B1 (en) 1997-04-30 2003-02-25 Regents Of The University Of Minnesota Hepatocellular chimeraplasty
JP2002511851A (ja) 1997-04-30 2002-04-16 リージェンツ オブ ジ ユニバーシティー オブ ミネソタ 肝細胞の遺伝的病変を修正するためのリコンビナジェニックオリゴヌクレオ塩基のｉｎ ｖｉｖｏでの使用
KR20010022652A (ko) 1997-08-05 2001-03-26 키메라젠, 인크. 식물에서 국소적인 유전자변형을 유발하기 위한 혼성 이중나선올리고뉴클레오티드의 용도
CN1191364C (zh) 1997-10-07 2005-03-02 巴西农业研究公司 获得含有外源dna的转基因豆科植物(豆科)的方法
WO1999043797A2 (en) 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
US6004804A (en) 1998-05-12 1999-12-21 Kimeragen, Inc. Non-chimeric mutational vectors
US6010907A (en) 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
AU5336999A (en) * 1998-08-05 2000-02-28 Trustees Of Tufts College Novel rrn operon altered bacteria and uses thereof
US6271360B1 (en) 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
WO2001025460A2 (en) 1999-10-07 2001-04-12 Valigen, Inc. Compositions and methods for plant genetic modification
US6936467B2 (en) * 2000-03-27 2005-08-30 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides
US20030115641A1 (en) 2001-07-24 2003-06-19 Dobres Michael S. Transformation of plants by electroporation of cultured explants
WO2007073149A1 (en) 2005-12-22 2007-06-28 Keygene N.V. Alternative nucleotides for improved targeted nucleotide exchange
RU2441366C2 (ru) * 2006-01-12 2012-02-10 Сайбас Юроп Б.В. Мутанты epsps
AU2008258254B2 (en) 2007-06-07 2014-07-03 Agriculture And Agri-Food Canada Nanocarrier based plant transfection and transduction
BR122018070230B1 (pt) * 2007-10-05 2023-01-10 Cibus Europe B.V. Método para produção de planta resistente à herbicida
AU2009259882A1 (en) 2008-06-20 2009-12-23 University Of Georgia Research Foundation, Inc. Development of herbicide-resistant grass species
EP2376637A1 (en) * 2008-12-22 2011-10-19 Keygene N.V. Use of double stranded rna to increase the efficiency of targeted gene alteration in plant protoplasts
EP2462230B1 (en) * 2009-08-03 2015-07-15 Recombinetics, Inc. Methods and compositions for targeted gene modification
ES2625154T3 (es) 2009-11-23 2017-07-18 Nucelis Llc Métodos y composiciones para producir escualeno empleando levaduras
WO2011117249A1 (en) * 2010-03-22 2011-09-29 Philip Morris Products S.A. Modifying enzyme activity in plants
US9512444B2 (en) * 2010-07-23 2016-12-06 Sigma-Aldrich Co. Llc Genome editing using targeting endonucleases and single-stranded nucleic acids
UA112969C2 (uk) * 2010-08-03 2016-11-25 Сібас Юс Ллс Рослина, стійка до одного або більше ррх-інгібуючих гербіцидів, яка містить мутантний ген протопорфіриноген ix оксидази (ррх)
US20120122223A1 (en) * 2010-08-03 2012-05-17 Cibus Us Llc Mutated protoporphyrinogen ix oxidase (ppx) genes
CA2871524C (en) 2012-05-07 2021-07-27 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
DK3401400T3 (da) * 2012-05-25 2019-06-03 Univ California Fremgangsmåder og sammensætninger til rna-styret mål-dna-modifikation og til rna-styret transskriptionsmodulering
KR102240135B1 (ko) 2012-12-13 2021-04-14 다우 아그로사이언시즈 엘엘씨 부위 특이적 뉴클레아제 활성에 대한 dna 검출 방법
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
EA201591445A1 (ru) 2013-03-15 2016-03-31 Сибас Юс Ллс Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов

Also Published As

Publication number Publication date
CN105431039B (zh) 2018-10-26
US10287594B2 (en) 2019-05-14
WO2014144951A1 (en) 2014-09-18
KR20220139435A (ko) 2022-10-14
JP7170807B2 (ja) 2022-11-14
IL269368B (en) 2022-03-01
BR112015022829B1 (pt) 2022-06-14
CL2015002648A1 (es) 2016-03-28
IL269368A (en) 2019-11-28
AU2023216834A1 (en) 2023-09-28
IL241143B (en) 2019-09-26
JP7532473B2 (ja) 2024-08-13
HK1222507A1 (zh) 2017-07-07
KR102339732B1 (ko) 2021-12-15
LT3527068T (lt) 2022-08-10
JP6947784B2 (ja) 2021-10-13
BR112015022829A2 (pt) 2017-11-07
CA2905391C (en) 2023-09-12
KR20150128854A (ko) 2015-11-18
EP3527068A1 (en) 2019-08-21
KR102207728B1 (ko) 2021-01-27
EP2966980A4 (en) 2016-12-28
AU2014233519B2 (en) 2020-05-14
IL307456A (en) 2023-12-01
KR20240036153A (ko) 2024-03-19
JP6591394B2 (ja) 2019-10-16
EA201591445A1 (ru) 2016-03-31
CA2905391A1 (en) 2014-09-18
US20190264219A1 (en) 2019-08-29
JP2022023040A (ja) 2022-02-07
IL290273A (en) 2022-04-01
CN108823238B (zh) 2022-02-01
EP4136963A1 (en) 2023-02-22
CN108823238A (zh) 2018-11-16
NZ711145A (en) 2021-08-27
CA3206344A1 (en) 2014-09-18
NZ751577A (en) 2021-08-27
US11421240B2 (en) 2022-08-23
KR102647792B1 (ko) 2024-03-14
IL290273B1 (en) 2023-11-01
EP2966980A1 (en) 2016-01-20
IL290273B2 (en) 2024-03-01
PT3527068T (pt) 2022-07-04
CN105431039A (zh) 2016-03-23
AU2014233519A1 (en) 2015-09-03
IL241143A0 (en) 2015-11-30
NZ751574A (en) 2021-08-27
SI3527068T1 (sl) 2022-10-28
UA120033C2 (uk) 2019-09-25
PL3527068T3 (pl) 2022-10-10
ES2921207T3 (es) 2022-08-19
US20160289691A1 (en) 2016-10-06
ZA201902245B (en) 2020-12-23
AU2020217388A1 (en) 2020-09-03
RS63427B1 (sr) 2022-08-31
JP2020010706A (ja) 2020-01-23
KR20210154873A (ko) 2021-12-21
KR20210010954A (ko) 2021-01-28
MY183741A (en) 2021-03-10
HUE059269T2 (hu) 2022-11-28
AU2020217388B2 (en) 2023-09-07
JP2016512695A (ja) 2016-05-09
KR102451570B1 (ko) 2022-10-06
JP2023011803A (ja) 2023-01-24
ZA201507555B (en) 2020-01-29
EP3527068B1 (en) 2022-06-01
HRP20220803T1 (hr) 2022-09-30

Similar Documents

Publication Publication Date Title
DK3527068T3 (da) Fremgangsmåder og sammensætninger til forøgelse af effektivitet af målrettet genmodifikation under anvendelse af oligonukleotid-medieret genreparation
DK3116305T3 (da) Fremgangsmåder og sammensætninger til forøgelse af effektiviteten af målrettet genmodifikation ved anvendelse af oligonukleotidmedieret genreparation
DK2978419T3 (da) Sammensætninger til forbedring af cellelevedygtighed og fremgangsmåder til anvendelse deraf
DK2970456T3 (da) Fremgangsmåder og sammensætninger til levering af mrna-kodede antistoffer
DK3008168T3 (da) Sc-celler og sammensætninger og fremgangsmåde til dannelse deraf
DK3556869T3 (da) Sammensætninger og fremgangsmåder til sekventering af polynukleotider
DK2991646T3 (da) Fremgangsmåder og sammensætninger til forøgelse af cd4+-regulatoriske t-celler
DK2971167T3 (da) Sammensætninger og fremgangsmåder til targeting af nucleinsyre med nucleinsyrer
BR112015023679A2 (pt) composições e métodos para imunoterapia
DK2949204T4 (da) Målrettede strategier til forbedring af fænotypiske træk
DK2968099T3 (da) Metoder og sammensætninger til rengøring og desinfektion af overflader
DK2739293T3 (da) Fremgangsmåder og sammensætninger til frembringelse af vaccinavirus
DK3685848T3 (da) Sammensætninger og fremgangsmåder til behandling af pulmonal hypertension
DK2933181T3 (da) Fremgangsmåde til vedligeholdelse af flydende indretning til generering af vindkraft
DK3065771T3 (da) Fremgangsmåder og sammensætninger til vedvarende immunterapi
DK3384902T3 (da) Fremgangsmåder, sammensætninger og indretninger til opfyldelse af diætmæssige behov for fedtsyre
DK2830654T3 (da) Metoder og sammensætninger til behandling af inflammation
DK2955757T3 (da) Nitrid-effektkomponent og fremgangsmåde til fremstilling deraf
DK2966984T3 (da) Målrettet genmodifikation under anvendelse af oligonukleotid-medieret genreparation
DK2976072T3 (da) Epinephrinfinpartikler og fremgangsmåder til anvendelse deraf til behandling af tilstande, der reagerer på epinephrin
DK2965026T3 (da) Generering af slushice
DK2968377T3 (da) Sammensætninger og fremgangsmåder til behandling af en infektionssygdom
DK2970915T3 (da) Fremgangsmåder og sammensætninger til generering af stabile transficerede celler
DK2956543T3 (da) Sammensætninger og fremgangsmåder til behandling og reparation af sener
DK2858989T3 (da) Amorf form af en AKT-hæmmende pyrimidinyl-cyclopentan-forbindelse, sammensætninger deraf og fremgangsmåder dertil