DK3250703T3 - Ligeringsanalyser i væskefase - Google Patents
Ligeringsanalyser i væskefase Download PDFInfo
- Publication number
- DK3250703T3 DK3250703T3 DK16744000.7T DK16744000T DK3250703T3 DK 3250703 T3 DK3250703 T3 DK 3250703T3 DK 16744000 T DK16744000 T DK 16744000T DK 3250703 T3 DK3250703 T3 DK 3250703T3
- Authority
- DK
- Denmark
- Prior art keywords
- liquid
- analysis
- phase
- liquid phase
- liquid analysis
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/307—Single strand endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562108161P | 2015-01-27 | 2015-01-27 | |
| US14/788,670 US9856521B2 (en) | 2015-01-27 | 2015-06-30 | Ligation assays in liquid phase |
| PCT/US2016/014999 WO2016123154A1 (en) | 2015-01-27 | 2016-01-26 | Ligation assays in liquid phase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3250703T3 true DK3250703T3 (da) | 2022-06-27 |
Family
ID=56544256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16744000.7T DK3250703T3 (da) | 2015-01-27 | 2016-01-26 | Ligeringsanalyser i væskefase |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9856521B2 (da) |
| EP (2) | EP3250703B1 (da) |
| JP (1) | JP6768706B2 (da) |
| CN (1) | CN108112258A (da) |
| AU (1) | AU2016211632B9 (da) |
| CA (1) | CA2975088A1 (da) |
| DK (1) | DK3250703T3 (da) |
| ES (1) | ES2922645T3 (da) |
| GB (2) | GB2547369A (da) |
| PT (1) | PT3250703T (da) |
| SG (1) | SG11201706086XA (da) |
| WO (1) | WO2016123154A1 (da) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013106807A1 (en) | 2012-01-13 | 2013-07-18 | Curry John D | Scalable characterization of nucleic acids by parallel sequencing |
| US20230313309A1 (en) * | 2014-09-08 | 2023-10-05 | BioSpyder Technologies, Inc. | Methods for Nucleic Acid Sequence Detection |
| US10683534B2 (en) | 2015-01-27 | 2020-06-16 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| US11091810B2 (en) * | 2015-01-27 | 2021-08-17 | BioSpyder Technologies, Inc. | Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology |
| EP4119677B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US11118216B2 (en) * | 2015-09-08 | 2021-09-14 | Affymetrix, Inc. | Nucleic acid analysis by joining barcoded polynucleotide probes |
| US20190323074A1 (en) | 2017-01-05 | 2019-10-24 | Tervisetehnoloogiate Arenduskeskus As | Quantifying dna sequences |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| KR20210102324A (ko) * | 2018-12-10 | 2021-08-19 | 콤비네티 인코포레이티드 | 시료 디지털화를 위한 마이크로유체 어레이 |
| US20220064630A1 (en) | 2018-12-10 | 2022-03-03 | 10X Genomics, Inc. | Resolving spatial arrays using deconvolution |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| WO2020236939A2 (en) | 2019-05-23 | 2020-11-26 | Paradigm Diagnostics | Tissue preparation using nuclease |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| EP4025711A2 (en) | 2019-11-08 | 2022-07-13 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| ES2946357T3 (es) | 2019-12-23 | 2023-07-17 | 10X Genomics Inc | Métodos para el análisis espacial usando ligación con molde de ARN |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| ES2965354T3 (es) | 2020-04-22 | 2024-04-12 | 10X Genomics Inc | Métodos para análisis espacial que usan eliminación de ARN elegido como diana |
| EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| WO2021237087A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| WO2021252591A1 (en) | 2020-06-10 | 2021-12-16 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
| WO2021263111A1 (en) | 2020-06-25 | 2021-12-30 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| EP4060049B1 (en) * | 2021-03-18 | 2023-08-23 | Genomill Health Oy | Methods for accurate parallel quantification of nucleic acids in dilute or non-purified samples |
| DK4060050T3 (da) * | 2021-03-18 | 2023-03-27 | Genomill Health Oy | Meget følsomme fremgangsmåder til nøjagtig parallelkvantificering af nukleinsyrer |
| EP4196605A1 (en) | 2021-09-01 | 2023-06-21 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| EP4215619A1 (en) * | 2022-01-21 | 2023-07-26 | Genomill Health Oy | Methods for sensitive and accurate parallel quantification of nucleic acids |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5225324A (en) * | 1987-04-24 | 1993-07-06 | Bioscience International, Inc. | Diagnostics for mycobacteria in public health, medical, and veterinary practice |
| US4988617A (en) * | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| US7045289B2 (en) * | 1991-09-09 | 2006-05-16 | Third Wave Technologies, Inc. | Detection of RNA Sequences |
| SE9400522D0 (sv) * | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| WO1996034983A1 (en) * | 1995-05-05 | 1996-11-07 | The Perkin-Elmer Corporation | Methods and reagents for combined pcr amplification and hybridization probing assay |
| WO1997023647A1 (en) * | 1995-12-22 | 1997-07-03 | Behringwerke Aktiengesellschaft | Homogeneous amplification and detection of nucleic acids |
| EP1736554B1 (en) | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US20030083273A1 (en) * | 1999-04-20 | 2003-05-01 | Tod M. Woolf | Antisense oligomers |
| US7585632B2 (en) * | 1999-10-29 | 2009-09-08 | Hologic, Inc. | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US6368801B1 (en) * | 2000-04-12 | 2002-04-09 | Molecular Staging, Inc. | Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase |
| US7361489B2 (en) * | 2001-10-15 | 2008-04-22 | Mount Sinai School Of Medicine Of New York University | Nucleic acid amplification methods |
| US7727720B2 (en) * | 2002-05-08 | 2010-06-01 | Ravgen, Inc. | Methods for detection of genetic disorders |
| AU2003235894A1 (en) * | 2002-05-08 | 2003-12-12 | Arkray, Inc. | Method of detecting target nucleic acid |
| US20070065816A1 (en) * | 2002-05-17 | 2007-03-22 | Affymetrix, Inc. | Methods for genotyping |
| CN1506471A (zh) | 2002-12-09 | 2004-06-23 | 核酸检测方法 | |
| DE602004020220D1 (de) * | 2003-06-17 | 2009-05-07 | Keygene Nv | Mittel und verfahren zum nachweis von zielnukleotidsequenzen unter verwendung von ligationstests mit verbesserten oligonukleotidsondenpaaren |
| EP1831401B1 (en) * | 2004-12-29 | 2010-02-10 | Applied Biosystems, LLC | Methods, compositions, and kits for forming self-complementary polynucleotides |
| JP2008533974A (ja) | 2005-01-21 | 2008-08-28 | サード・ウェーブ・テクノロジーズ・インク | 核酸分子の増大したダイナミックレンジ検出のための組成物および方法 |
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| CA2622649C (en) * | 2007-03-09 | 2018-04-24 | Riken | Nucleic acid amplification method using primer exhibiting exciton effect |
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| US9487839B2 (en) * | 2009-09-29 | 2016-11-08 | Case Western Reserve University | Method for detecting single nucleotide polymorphisms |
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| US20130261003A1 (en) * | 2010-08-06 | 2013-10-03 | Ariosa Diagnostics, In. | Ligation-based detection of genetic variants |
| JP5951755B2 (ja) | 2011-05-04 | 2016-07-13 | エイチティージー モレキュラー ダイアグノスティクス， インコーポレイテッド | 定量的ヌクレアーゼプロテクションアッセイ（ｑＮＰＡ）法および定量的ヌクレアーゼプロテクション配列決定（ｑＮＰＳ）法の改善 |
| CN106434871B (zh) * | 2011-05-17 | 2020-08-18 | 德克斯特里蒂诊断公司 | 用于检测目标核酸的方法与组合物 |
| GB201116131D0 (en) * | 2011-09-19 | 2011-11-02 | Epistem Ltd | Probe |
| AU2013221480B2 (en) * | 2012-02-14 | 2018-08-23 | Cornell University | Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions |
| JP6234463B2 (ja) * | 2012-09-10 | 2017-11-22 | ジーンスカイ ダイアグノスティクス（スーツォウ） インコーポレーテッドＧｅｎｅｓｋｙ Ｄｉａｇｎｏｓｔｉｃｓ （Ｓｕｚｈｏｕ） Ｉｎｃ． | 核酸の多重分析の方法 |
| KR101984700B1 (ko) * | 2012-11-19 | 2019-05-31 | 삼성전자주식회사 | 폴리뉴클레오티드 및 그의 용도 |
| EP2976435B1 (en) * | 2013-03-19 | 2017-10-25 | Directed Genomics, LLC | Enrichment of target sequences |
| JP2016520326A (ja) | 2013-06-07 | 2016-07-14 | アテナ ダイアグナスティクス，インコーポレイテッド | マルチプレックス配列決定のための分子バーコード化 |
| US9957550B2 (en) | 2014-09-08 | 2018-05-01 | BioSpyder Technologies, Inc. | Attenuators |
| US10683534B2 (en) | 2015-01-27 | 2020-06-16 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| US9938566B2 (en) | 2014-09-08 | 2018-04-10 | BioSpyder Technologies, Inc. | Profiling expression at transcriptome scale |
-
2015
- 2015-06-30 US US14/788,670 patent/US9856521B2/en active Active
-
2016
- 2016-01-26 ES ES16744000T patent/ES2922645T3/es active Active
- 2016-01-26 SG SG11201706086XA patent/SG11201706086XA/en unknown
- 2016-01-26 CA CA2975088A patent/CA2975088A1/en active Pending
- 2016-01-26 DK DK16744000.7T patent/DK3250703T3/da active
- 2016-01-26 EP EP16744000.7A patent/EP3250703B1/en active Active
- 2016-01-26 JP JP2017558365A patent/JP6768706B2/ja active Active
- 2016-01-26 PT PT167440007T patent/PT3250703T/pt unknown
- 2016-01-26 AU AU2016211632A patent/AU2016211632B9/en active Active
- 2016-01-26 GB GB1707052.5A patent/GB2547369A/en not_active Withdrawn
- 2016-01-26 CN CN201680018676.0A patent/CN108112258A/zh active Pending
- 2016-01-26 WO PCT/US2016/014999 patent/WO2016123154A1/en active Application Filing
- 2016-01-26 EP EP22173117.7A patent/EP4134441A1/en active Pending
- 2016-01-26 GB GB1614871.0A patent/GB2542929B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| SG11201706086XA (en) | 2017-08-30 |
| GB201707052D0 (en) | 2017-06-14 |
| EP3250703B1 (en) | 2022-06-08 |
| CN108112258A (zh) | 2018-06-01 |
| EP3250703A1 (en) | 2017-12-06 |
| US20160222447A1 (en) | 2016-08-04 |
| GB201614871D0 (en) | 2016-10-19 |
| AU2016211632A2 (en) | 2017-08-31 |
| JP2018503403A (ja) | 2018-02-08 |
| PT3250703T (pt) | 2022-09-19 |
| GB2542929A (en) | 2017-04-05 |
| US9856521B2 (en) | 2018-01-02 |
| AU2016211632B2 (en) | 2021-03-11 |
| CA2975088A1 (en) | 2016-08-04 |
| AU2016211632B9 (en) | 2021-03-25 |
| EP4134441A1 (en) | 2023-02-15 |
| AU2016211632A1 (en) | 2017-08-24 |
| WO2016123154A1 (en) | 2016-08-04 |
| JP6768706B2 (ja) | 2020-10-14 |
| EP3250703A4 (en) | 2018-08-01 |
| ES2922645T3 (es) | 2022-09-19 |
| GB2542929B (en) | 2017-08-02 |
| GB2547369A (en) | 2017-08-16 |
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