DK3227430T3 - Svampeværtsstammer, dna-konstruktioner og fremgangsmåder til anvendelse - Google Patents

Svampeværtsstammer, dna-konstruktioner og fremgangsmåder til anvendelse Download PDF

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Publication number
DK3227430T3
DK3227430T3 DK15808523.3T DK15808523T DK3227430T3 DK 3227430 T3 DK3227430 T3 DK 3227430T3 DK 15808523 T DK15808523 T DK 15808523T DK 3227430 T3 DK3227430 T3 DK 3227430T3
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DK
Denmark
Prior art keywords
starms
methods
dna constructions
fungi host
fungi
Prior art date
Application number
DK15808523.3T
Other languages
English (en)
Inventor
Mark Arentshorst
Sharief Barends
Paulien Kruithof
Igor Nikolaev
Jean Paul Ouedraogo
Arthur F J Ram
Original Assignee
Danisco Us Inc
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Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of DK3227430T3 publication Critical patent/DK3227430T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK15808523.3T 2014-12-01 2015-12-01 Svampeværtsstammer, dna-konstruktioner og fremgangsmåder til anvendelse DK3227430T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462085834P 2014-12-01 2014-12-01
PCT/US2015/063095 WO2016089815A1 (en) 2014-12-01 2015-12-01 Fungal host strains, dna constructs, and methods of use

Publications (1)

Publication Number Publication Date
DK3227430T3 true DK3227430T3 (da) 2021-08-09

Family

ID=54849725

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15808523.3T DK3227430T3 (da) 2014-12-01 2015-12-01 Svampeværtsstammer, dna-konstruktioner og fremgangsmåder til anvendelse

Country Status (7)

Country Link
US (1) US20170260559A1 (da)
EP (1) EP3227430B1 (da)
JP (1) JP6830891B2 (da)
KR (1) KR102489871B1 (da)
CN (1) CN107109424A (da)
DK (1) DK3227430T3 (da)
WO (1) WO2016089815A1 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753631B (zh) * 2018-07-10 2021-07-09 潍坊康地恩生物科技有限公司 一种里氏木霉及其在单宁酶生产中的应用
CN113223606B (zh) * 2021-05-13 2022-05-27 浙江大学 一种用于复杂性状遗传改良的基因组选择方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958727A (en) * 1996-09-13 1999-09-28 Novo Nordisk Biotech, Inc Methods for modifying the production of a polypeptide
US6436643B1 (en) * 1997-12-22 2002-08-20 Unilever Patent Holdings Bv Process for site-directed integration of multiple copies of a gene in a mould
DE10159396A1 (de) * 2001-12-04 2003-06-12 Basf Ag Genetische Stammoptimierung zur verbesserten Herstellung von Riboflavin
US8138321B2 (en) 2006-09-22 2012-03-20 Danisco Us Inc. Acetolactate synthase (ALS) selectable marker from Trichoderma reesei
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
KR101768225B1 (ko) 2008-03-07 2017-08-14 다니스코 유에스 인크. 트리코데르마에서의 카탈라아제 발현
WO2011153449A1 (en) * 2010-06-03 2011-12-08 Danisco Us Inc. Filamentous fungal host strains and dna constructs, and methods of use thereof
CN101892221A (zh) * 2010-06-30 2010-11-24 苏州神洲基因有限公司 染色体的无痕迹修饰方法
CN103060208B (zh) * 2012-05-31 2014-07-23 山东省科学院中日友好生物技术研究中心 高效表达β-1,4-葡聚糖酶编码基因的木霉工程菌株及其应用
EP3321353A1 (en) * 2012-12-11 2018-05-16 Danisco US Inc. Yeast host cells epxressing a glucoamylase from aspergillus fumigatus and methods of use thereof

Also Published As

Publication number Publication date
KR20170088964A (ko) 2017-08-02
WO2016089815A1 (en) 2016-06-09
EP3227430A1 (en) 2017-10-11
CN107109424A (zh) 2017-08-29
EP3227430B1 (en) 2021-05-19
JP2018500893A (ja) 2018-01-18
US20170260559A1 (en) 2017-09-14
JP6830891B2 (ja) 2021-02-17
KR102489871B1 (ko) 2023-01-17

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