DK3108016T3 - Pentosefermenterende stamme med optimeret formering - Google Patents

Pentosefermenterende stamme med optimeret formering Download PDF

Info

Publication number
DK3108016T3
DK3108016T3 DK15709252.9T DK15709252T DK3108016T3 DK 3108016 T3 DK3108016 T3 DK 3108016T3 DK 15709252 T DK15709252 T DK 15709252T DK 3108016 T3 DK3108016 T3 DK 3108016T3
Authority
DK
Denmark
Prior art keywords
strain
xylose
medium
yeast
source
Prior art date
Application number
DK15709252.9T
Other languages
English (en)
Inventor
Thomas Desfougeres
Georges Pignede
Jennifer Techel
Original Assignee
Lesaffre & Cie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lesaffre & Cie filed Critical Lesaffre & Cie
Application granted granted Critical
Publication of DK3108016T3 publication Critical patent/DK3108016T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01006—Galactokinase (2.7.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (14)

1. Fremgangsmåde til tilvejebringelse af en gærstamme, som er egnet til at formeres effektivt på et medie med lavt næringsmæssigt potentiale, som er egnet til at meta-bolisere pentoser, og med resistens mod fermenteringsinhibitorer, som er til stede ab initio i lignocellulose-hydrolysater eller dannede under alkoholisk fermentering, omfattende følgende trin: a) krydsning af en rekombinant gærstamme, som er egnet til at metabolisere pentoser og til at modstå fermenteringsinhibitorer, med en vild gærstamme, som er uden mangler og egnede til at vokse på et minimummedie, hvor den rekombinante gærstamme omfatter i det mindste én kopi af et exogent xyloseisomerasegen og i det mindste en yderligere kopi af et D-xylulokinasegen inkorporeret i genomet i en region genetisk forbundet med et ekspressionslokus af sexuel art og som fraspaltes samlet, b) i det mindste to cycluser med genomblanding ved tilfældig sporulation og/eller hybri-disering, uden udvælgelse imellem disse, c) udvælgelse af populationen tilvejebragt i trin b) i overensstemmelse med egnet-hedskriterie for stammerne til xylosemetabolisering, d) udvælgelse af populationen tilvejebragt i trin c) i overensstemmelse med et egnet-hedskriterie for stammerne til at vokse på et medie med lav næringsværdi, som er med lavt nitrogenbaseindhold og som omfatter xylose som en eneste kilde for carbon, en kilde for mineralsk nitrogen, en kilde for kalium, en kilde for phosphor, en kilde for svovl, en kilde for magnesium, en kilde for calcium, en kilde for jern, en kilde for mikronæringsstoffer og vand, idet det bemærkes, at de to udvælgelsestrin kan ombyttes.
2. Fremgangsmåde ifølge krav 1, hvor den rekombinante gærstamme implementeret i trin a) er gærstammen deponeret ved CNCM (National Collection of Microorganism Cultures, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15) under nummeret I-4538.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor trin c) omfatter et trin med udvælgelse på et YFX medie som defineret i tabel I.
4. Fremgangsmåde ifølge ethvert af kravene 1 til 3, hvor trin c) omfatter trinnene med: måling af procentdelen af xylose konverteret til ethanol af i det mindste én hybrid, under anærobe betingelser over 60 timer i et fermenteringsmedie omfattende 55 g glucose og 45 g xylose pr. kg af det nævnte medie og udvælgelse af i det mindste én hybrid, som konverterer i det mindste 70% af xylosen til ethanol på 60 timer.
5. Fremgangsmåde ifølge ethvert af kravene 1 til 4, hvor trin d) består i måling af formeringen af stammen på et medie med lav næringsværdi, som omfatter et proteinhydro-lysat og som mangler nitrogene baser.
6. Fremgangsmåde ifølge krav 5, hvor mediet med lav næringsværdi er det Pref-medie, som er defineret i tabel I.
7. Fremgangsmåde ifølge krav 1, som omfatter et yderligere trin med genetisk transformation ved tilføjelsen af i det mindste én kopi af GAZ_2-genet.
8. Gærstamme, egnet til at metabolisere pentoser og med resistens mod fermenteringsinhibitorer, som omfatter i det mindste én kopi af et exogent xyloseisomerasegen og i det mindste én yderligere kopi af et D-xylulokinasegen inkorporeret i genomet og forbundet med et enkelt af de sexuelle træk for stammen, egnet til at vokse på et medie med lav næringsværdi, som omfatter et proteinhydrolysat og som mangler nitrogene baser, som kan opnås ved fremgangsmåden ifølge ethvert af kravene 1 til 6, hvilken gær er af arten Saccharomyces cerevisiae, og som er deponeret ved CNCM (National Collection of Cultures of Microorganisms, Pasteur Institute, 25 rue du Docteur Roux, 75724 Paris Cedex 15) under nummmeret i-4749.
9. Gærstamme opnået fra stammen ifølge krav 8, ved indføring af i det mindste én yderligere kopi af GAZ_2-genet.
10. Gærstamme ifølge krav 9, hvilken stamme er af arten Saccharomyces cerevisiae og som er deponeret ved CNCM (National Collection of Cultures of Microorganisms, Pasteur Institute, 25 rue du Docteur Roux, 75724 Paris Cedex 15) under nummeret I-4829.
11. Kultur omfattende formeringen af en gærstamme ifølge ethvert af kravene 8 til 10.
12. Fremgangsmåde til fremstilling af i det mindste ét fermenteringsprodukt omfattende følgende trin: a) fermentering, under anærobe eller semi-anærobe betingelser, af et medie omfattende en kilde for xylose, med en gærstamme ifølge ethvert af kravene 8 til 10 eller en kultur ifølge krav 11, b) opnåelse af ethanol.
13. Anvendelse af en gærstamme ifølge ethvert af kravene 8 til 10 eller en kultur ifølge krav 11 til konverteringen og metaboliseringen af et materiale af vegetabilsk oprindelse, som omfatter xylose.
14. Anvendelse ifølge krav 13 til fremstillingen af ethanol.
DK15709252.9T 2014-02-17 2015-02-16 Pentosefermenterende stamme med optimeret formering DK3108016T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1451246A FR3017623B1 (fr) 2014-02-17 2014-02-17 Souche fermentant les pentoses a propagation optimisee
PCT/FR2015/050364 WO2015121595A1 (fr) 2014-02-17 2015-02-16 Souche fermentant les pentoses a propagation optimisée

Publications (1)

Publication Number Publication Date
DK3108016T3 true DK3108016T3 (da) 2018-11-26

Family

ID=51063547

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15709252.9T DK3108016T3 (da) 2014-02-17 2015-02-16 Pentosefermenterende stamme med optimeret formering

Country Status (16)

Country Link
US (1) US10273447B2 (da)
EP (1) EP3108016B1 (da)
JP (1) JP6653659B2 (da)
CN (1) CN106414735B (da)
AU (1) AU2015216821B2 (da)
BR (1) BR112016017881B1 (da)
CA (1) CA2938962C (da)
DK (1) DK3108016T3 (da)
ES (1) ES2695728T3 (da)
FR (1) FR3017623B1 (da)
HR (1) HRP20181793T1 (da)
HU (1) HUE040429T2 (da)
MY (1) MY191221A (da)
PL (1) PL3108016T3 (da)
PT (1) PT3108016T (da)
WO (1) WO2015121595A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3062134B1 (fr) 2017-01-24 2023-07-21 Lesaffre & Cie Obtention de souches de levure performantes pour la metabolisation de l'arabinose
WO2019046750A1 (en) 2017-09-01 2019-03-07 Swimc Llc MULTI-STAGE POLYMER LATEX, COATING COMPOSITIONS CONTAINING SUCH LATEX, AND ARTICLES COATED THEREWITH
WO2019046700A1 (en) 2017-09-01 2019-03-07 Swimc Llc MULTI-STAGE POLYMER LATEX, COATING COMPOSITIONS CONTAINING SUCH LATEX, AND ARTICLES THEREOF
CN110734937A (zh) * 2018-07-20 2020-01-31 远东新世纪股份有限公司 用于生产乳酸的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1766007B1 (en) * 2004-06-08 2011-10-05 Microbiogen Pty Ltd Non-recombinant saccharomyces strains that grow on xylose
DE102008031350B4 (de) 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen
US8440449B2 (en) * 2008-09-30 2013-05-14 The United States Of America, As Represented By The Secretary Of Agriculture Transformed Saccharomyces cerevisiae engineered for xylose utilization
BR112012018764A2 (pt) * 2009-12-30 2015-09-15 Iogen Energy Corp cepas de levedura modificadas exibindo co-fermentação melhorada de xilose e glicose em hidrolisados de lignocelulose
FR2968313B1 (fr) * 2010-12-03 2014-10-10 Lesaffre & Cie Procede de preparation d'une levure industrielle, levure industrielle et application a la production d'ethanol a partir d'au moins un pentose
US20140322776A1 (en) * 2011-11-29 2014-10-30 Codexis, Inc. Overexpression of genes that improve fermentation in yeast using cellulosic substrates
FR2991340B1 (fr) * 2012-06-01 2016-02-19 Lesaffre & Cie Procede d'obtention de souches de levure ameliorees par modification genetique et croisement
FR2991339B1 (fr) * 2012-06-01 2016-02-19 Lesaffre & Cie Souches de levure aptes a metaboliser le xylose et resistantes a au moins un inhibiteur de fermentation, procede d'obtention et utilisation

Also Published As

Publication number Publication date
JP6653659B2 (ja) 2020-02-26
FR3017623A1 (fr) 2015-08-21
AU2015216821B2 (en) 2021-07-08
CA2938962A1 (en) 2015-08-20
HUE040429T2 (hu) 2019-03-28
US10273447B2 (en) 2019-04-30
WO2015121595A1 (fr) 2015-08-20
ES2695728T3 (es) 2019-01-10
EP3108016A1 (fr) 2016-12-28
CN106414735A (zh) 2017-02-15
US20160348064A1 (en) 2016-12-01
AU2015216821A1 (en) 2016-08-04
BR112016017881A2 (pt) 2017-10-17
CN106414735B (zh) 2021-03-16
EP3108016B1 (fr) 2018-10-24
PT3108016T (pt) 2018-11-21
PL3108016T3 (pl) 2019-01-31
BR112016017881B1 (pt) 2023-04-11
HRP20181793T1 (hr) 2018-12-28
CA2938962C (en) 2022-07-19
FR3017623B1 (fr) 2018-08-10
MY191221A (en) 2022-06-09
JP2017505627A (ja) 2017-02-23

Similar Documents

Publication Publication Date Title
JP5872520B2 (ja) キシロース上で生育する非組換えサッカロマイセス株
Demeke et al. Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering
DK2855711T3 (da) Gærstammer der er i stand til at nedbryde xylose og er resistente over for inhibitorer, fremgangsmåde til at opnå samme og anvendelse deraf
US9309524B2 (en) Method for preparing an industrial yeast, industrial yeast, and application to the production of ethanol from at least one pentose
DK3108016T3 (da) Pentosefermenterende stamme med optimeret formering
JP2018082704A (ja) Ｃ５糖を含む基質においてバイオマスを産生するための酵母菌株
TWI450963B (zh) 具高木醣消耗率之分離酵母菌株及使用該菌株製造酒精之方法
Neha Bisht et al. Batch fermentation of underutilized pear fruits using newly isolated stress tolerant ethanogenic yeast Pichia kudriavezeii BGY1.