DK2946007T3 - Reprogrammering af humant endothelium til hematopoietiske multilinje-stamceller af definerede faktorer - Google Patents

Reprogrammering af humant endothelium til hematopoietiske multilinje-stamceller af definerede faktorer Download PDF

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Publication number
DK2946007T3
DK2946007T3 DK14740745.6T DK14740745T DK2946007T3 DK 2946007 T3 DK2946007 T3 DK 2946007T3 DK 14740745 T DK14740745 T DK 14740745T DK 2946007 T3 DK2946007 T3 DK 2946007T3
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hematopoytic
multiline
stamps
defined factors
reprogramming human
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DK14740745.6T
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Vladislav M Sandler
Shahin Rafii
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Univ Cornell
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/28—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from vascular endothelial cells

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DK14740745.6T 2013-01-15 2014-01-15 Reprogrammering af humant endothelium til hematopoietiske multilinje-stamceller af definerede faktorer DK2946007T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361752688P 2013-01-15 2013-01-15
PCT/US2014/011575 WO2014113415A1 (en) 2013-01-15 2014-01-15 Reprogramming of human endothelium into hematopoietic multi-lineage progenitors by defined factors

Publications (1)

Publication Number Publication Date
DK2946007T3 true DK2946007T3 (da) 2019-07-15

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DK14740745.6T DK2946007T3 (da) 2013-01-15 2014-01-15 Reprogrammering af humant endothelium til hematopoietiske multilinje-stamceller af definerede faktorer

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Country Link
US (1) US10113149B2 (da)
EP (1) EP2946007B1 (da)
JP (1) JP6581906B2 (da)
CN (1) CN105051184B (da)
AU (1) AU2014207627B2 (da)
CA (1) CA2898180C (da)
DK (1) DK2946007T3 (da)
ES (1) ES2733909T3 (da)
RU (1) RU2691062C2 (da)
WO (1) WO2014113415A1 (da)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201210857D0 (en) 2012-06-19 2012-08-01 Cambridge Entpr Ltd Transcription factor mediated programming towards megakaryocytes
CA3177929A1 (en) 2012-12-21 2014-06-26 Astellas Institute For Regenerative Medicine Methods for production of platelets from pluripotent stem cells and compositions thereof
WO2014120714A1 (en) 2013-01-30 2014-08-07 Cornell University Compositions and methods for the expansion of stem cells
RU2745300C2 (ru) 2014-06-27 2021-03-23 Анджиокрин Биосайенс, Инк. Клетки нервной системы, экспрессирующие e4orf1 аденовируса, и способы их получения и применения
US20160243288A1 (en) 2015-02-23 2016-08-25 Tissuetech, Inc. Apparatuses and methods for treating ophthalmic diseases and disorders
WO2016187820A1 (zh) * 2015-05-26 2016-12-01 中国科学院广州生物医药与健康研究院 一种人原代细胞培养基及其应用
WO2016201133A2 (en) * 2015-06-09 2016-12-15 The Regents Of The University Of California Hematopoietic cells and methods of using and generating the same
US10961509B2 (en) 2015-07-20 2021-03-30 Angiocrine Bioscience, Inc. Engineered endothelial cells expressing an ETS transcription factor
KR20180048617A (ko) * 2015-07-20 2018-05-10 앤지오크린 바이오사이언스 인코포레이티드 줄기 세포 이식 방법 및 조성물
US11162073B2 (en) 2015-12-03 2021-11-02 The Brigham And Women's Hospital, Inc. Methods for generating functional hematopoietic stem cells
AU2016378989B2 (en) * 2015-12-23 2023-06-08 Mogrify Limited Cell reprogramming
RU2018139201A (ru) * 2016-04-15 2020-05-15 Анджиокрин Биосайенс, Инк. Улучшенные способы доставки генов
WO2017192708A1 (en) * 2016-05-03 2017-11-09 The Children's Medical Center Corporation Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells
KR102458639B1 (ko) 2016-09-13 2022-10-25 앤지오크린 바이오사이언스 인코포레이티드 조작된 내피 세포를 함유하는 혈액-뇌 장벽
JP7265988B2 (ja) * 2017-02-03 2023-04-27 コーネル ユニヴァーシティー 安定な３次元血管およびそれを形成するための方法
US11572544B2 (en) 2017-06-14 2023-02-07 The Children's Medical Center Corporation Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer
CN112105721A (zh) * 2018-03-23 2020-12-18 儿童医疗中心公司 内皮细胞因子及其方法
CN108676777A (zh) * 2018-04-03 2018-10-19 华南生物医药研究院 胎肝窦内皮细胞株在造血干细胞扩增和分化中的应用
CN110577967A (zh) * 2018-05-22 2019-12-17 中国人民解放军军事科学院军事医学研究院 诱导性多能干细胞及其制备方法
WO2020262609A1 (ja) * 2019-06-27 2020-12-30 昭和電工マテリアルズ株式会社 細胞足場材、細胞培養支持体、及び細胞の培養方法
EP3993812A4 (en) * 2019-07-03 2023-08-09 Cornell University PROCEDURE FOR FUNCTIONAL VASCULARISATION OF PANCREA ISLANDS AND BETA CELL ORGANOIDS
US20230256029A1 (en) * 2020-06-25 2023-08-17 Angiocrine Bioscience, Inc. Endothelial cells for mitigation of chemotherapy-induced toxicity
US20230113074A1 (en) * 2021-10-12 2023-04-13 Wisconsin Alumni Research Foundation Derivation of hepatocytes and hematopoietic progenitors from human embryonic stem cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2556018A1 (en) 2004-02-11 2005-09-09 Aldagen, Inc. Stem cell populations and methods of use
WO2005100549A1 (ja) * 2004-04-14 2005-10-27 Kanazawa University Technology Licensing Organization Ltd. 臓器特異的幹細胞の増殖方法及び増殖装置
RU2359030C1 (ru) * 2008-03-19 2009-06-20 Общество С Ограниченной Ответственностью "Лаборатория Клеточных Технологий" Способ получения эндотелиальных клеток из эмбриональных стволовых клеток человека (варианты)
JP5725394B2 (ja) 2009-03-25 2015-05-27 公立大学法人大阪府立大学 造血幹細胞の培養方法
EP2630232A4 (en) 2010-10-22 2014-04-02 Biotime Inc METHOD FOR MODIFYING TRANSCRIPTIONAL REGULATORY NETWORKS IN STEM CELLS
WO2012109208A2 (en) * 2011-02-08 2012-08-16 Cellular Dynamics International, Inc. Hematopoietic precursor cell production by programming

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Publication number Publication date
EP2946007A4 (en) 2016-06-08
US20150361398A1 (en) 2015-12-17
AU2014207627B2 (en) 2019-09-12
CN105051184B (zh) 2018-08-10
AU2014207627A1 (en) 2015-08-06
JP2016505266A (ja) 2016-02-25
EP2946007A1 (en) 2015-11-25
US10113149B2 (en) 2018-10-30
CA2898180C (en) 2023-09-26
JP6581906B2 (ja) 2019-09-25
CN105051184A (zh) 2015-11-11
EP2946007B1 (en) 2019-04-10
ES2733909T3 (es) 2019-12-03
RU2691062C2 (ru) 2019-06-10
WO2014113415A1 (en) 2014-07-24
RU2015134394A (ru) 2017-02-21
CA2898180A1 (en) 2014-07-24

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