DK2620503T3 - Modificeret parvovirus anvendelig til genslukning - Google Patents
Modificeret parvovirus anvendelig til genslukning Download PDFInfo
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- DK2620503T3 DK2620503T3 DK12000554.1T DK12000554T DK2620503T3 DK 2620503 T3 DK2620503 T3 DK 2620503T3 DK 12000554 T DK12000554 T DK 12000554T DK 2620503 T3 DK2620503 T3 DK 2620503T3
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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Claims (16)
1. Gnaverparvovirus til nedregulering af ekspressionen af et målgen i en celle, kendetegnet ved, at det indeholder en målspecifik nukleinsyre, som er indført i en ikke-translateret region nedstrøms for parvovirus VP genet og kan eksprimeres under kontrol af en promotor eller promotorregion genkendelig for en RNA polymerase i cellen, hvori den målspecifikke nukleinsyre kan transkriberes i en RNAi, og hvori parvovirusset er i stand til at mangfoldiggøres og udbredes i cellen.
2. Parvovirus ifølge krav 1, hvori målgenet er et sygdomsfremkaldende gen.
3. Parvovirus ifølge krav 2, hvori det sygdomsfremkaldende gen er et patogent dyrevirusgen, en kræftrelateret gen, et onkogen, antiapoptotisk gen, et gen, som er kritisk for tumorcellevækst, metastasedannelse, angiogenese eller kemoresi-stens, et immunmodulatorisk gen eller et gen, der koder et cytokin, en vækstfaktor, et enzym eller en transkriptionsfaktor.
4. Parvovirus ifølge ethvert af de foregående krav, hvori gnavervirusset er Lull I, muse-minute-virus (MMV), muse-parvovirus (MPV), rotte-minute-virus (RMV), rotte-parvovirus (RPV), rotte-virus (RV) eller H1 (H-1PV).
5. Gnaverparvovirus ifølge krav 4, hvori den målspecifikke nukleinsyre er indført ved nukleotid 4683 af vildtype H-1 PV genomet.
6. Parvovirus ifølge ethvert af de foregående krav, hvori promotoren eller promotorregionen, som kan genkendes af en RNA polymerase af cellen, er en RNA-polymerase II (Pol II) eller III (Pol III) promotor.
7. Parvovirus ifølge krav 6, hvori RNA-polymerase III (Pol III) promotoren er RNA-polymerase III H1 promotoren.
8. Parvovirus ifølge ethvert af de foregående krav, hvori den målspecifikke nukleinsyre er en shRNA.
9. Parvovirus ifølge ethvert af de foregående krav, hvori den målspecifikke nukleinsyre har en længde på mindst 15 nukleotider.
10. Gnaverparvovirus ifølge ethvert af de foregående krav til anvendelse ved behandling af en sygdom forårsaget af et patogent dyrevirusgen, et kræftrelateret gen, et onkogen, antiapoptotisk gen, et gen, som er kritisk for tumorcellevækst, metastasedannelse, angiogenese eller kemoresistens, eller en sygdom knyttet til den afvigende ekspression af et immunmodulatorisk gen eller et gen, der koder et cytokin, en vækstfaktor, et enzym eller en transkriptionsfaktor.
11. Parvovirus ifølge ethvert af krav 1 til 9 til anvendelse ifølge krav 10, kendetegnet ved, at anvendelsen er til behandling af en tumor.
12. Parvovirus ifølge ethvert af krav 1 til 9 til anvendelse ifølge krav 11, kendetegnet ved, at tumoren er en hjernetumor.
13. Parvovirus ifølge ethvert af krav 1 til 9 til anvendelse ifølge krav 11 eller 12, kendetegnet ved, at tumorens celler er resistente overfor kemoterapi og/eller stråleterapi.
14. Parvovirus ifølge ethvert af krav 1 til 9 til anvendelse ifølge ethvert af krav 10 til 13, kendetegnet ved, at parvovirusset gives ved intravenøs (i.v.), intratumo-ral eller endobronkial indgivelse.
15. Celle indeholdende en parvovirus ifølge ethvert af krav 1 til 9.
16. Transgent ikke-humant dyr omfattende en celle ifølge krav 15.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12000554.1A EP2620503B1 (en) | 2012-01-27 | 2012-01-27 | Modified parvovirus useful for gene silencing |
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| DK2620503T3 true DK2620503T3 (da) | 2014-12-15 |
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| DK12000554.1T DK2620503T3 (da) | 2012-01-27 | 2012-01-27 | Modificeret parvovirus anvendelig til genslukning |
| DK13705388.0T DK2807259T3 (da) | 2012-01-27 | 2013-01-25 | Modificeret parvovirus til gen-inaktivering |
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| DK13705388.0T DK2807259T3 (da) | 2012-01-27 | 2013-01-25 | Modificeret parvovirus til gen-inaktivering |
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| EP (2) | EP2620503B1 (da) |
| JP (1) | JP5901796B2 (da) |
| AU (1) | AU2013211755B2 (da) |
| CA (1) | CA2849370C (da) |
| DK (2) | DK2620503T3 (da) |
| ES (2) | ES2526154T3 (da) |
| WO (1) | WO2013110464A1 (da) |
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| WO2015148683A1 (en) * | 2014-03-26 | 2015-10-01 | Tocagen Inc. | A retroviral vector having immune-stimulating activity |
| EP3327124A1 (en) | 2016-11-28 | 2018-05-30 | Deutsches Krebsforschungszentrum | H-1 pv expressing rnai effectors targeting cdk9 |
| EP3669893A1 (en) * | 2018-12-20 | 2020-06-24 | Universidad Autónoma de Madrid | Treatment of p53 mutated/modified cancer-forms with parvovirus |
| EP4074835A1 (en) | 2021-04-15 | 2022-10-19 | Deutsches Krebsforschungszentrum - Stiftung des öffentlichen Rechts / Universität Heidelberg | H-1 pv expressing rnai effectors |
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| US4873191A (en) | 1981-06-12 | 1989-10-10 | Ohio University | Genetic transformation of zygotes |
| CA2744959A1 (en) * | 2008-12-11 | 2010-06-17 | Psioxus Therapeutics Limited | Modification of nucleic acid vectors with polymers comprising charged quaternary amino groups |
| ES2461147T3 (es) * | 2010-03-26 | 2014-05-16 | Deutsches Krebsforschungszentrum | Virus cooperador obtenido a partir de adenovirus para mejorar la producción de parvovirus recombinantes |
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2012
- 2012-01-27 DK DK12000554.1T patent/DK2620503T3/da active
- 2012-01-27 ES ES12000554.1T patent/ES2526154T3/es active Active
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| US10227609B2 (en) | 2019-03-12 |
| ES2526154T3 (es) | 2015-01-07 |
| CA2849370C (en) | 2018-07-10 |
| DK2807259T3 (da) | 2017-04-03 |
| US20140310829A1 (en) | 2014-10-16 |
| JP2015506178A (ja) | 2015-03-02 |
| CA2849370A1 (en) | 2013-08-01 |
| JP5901796B2 (ja) | 2016-04-13 |
| EP2620503A1 (en) | 2013-07-31 |
| EP2620503B1 (en) | 2014-10-22 |
| AU2013211755B2 (en) | 2016-04-21 |
| EP2807259A1 (en) | 2014-12-03 |
| ES2618935T3 (es) | 2017-06-22 |
| WO2013110464A1 (en) | 2013-08-01 |
| AU2013211755A1 (en) | 2014-03-27 |
| EP2807259B1 (en) | 2016-12-21 |
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