DK2554677T3 - Levedygtig bakterieantals-målefremgangsmåde og dyrkningsmedium - Google Patents

Levedygtig bakterieantals-målefremgangsmåde og dyrkningsmedium Download PDF

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Publication number
DK2554677T3
DK2554677T3 DK11759568.6T DK11759568T DK2554677T3 DK 2554677 T3 DK2554677 T3 DK 2554677T3 DK 11759568 T DK11759568 T DK 11759568T DK 2554677 T3 DK2554677 T3 DK 2554677T3
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DK
Denmark
Prior art keywords
culture medium
species
bifidobacterium
microorganism
medium
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Application number
DK11759568.6T
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English (en)
Inventor
Masamichi Muto
Fumiaki Abe
Tomoko Yaeshima
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Morinaga Milk Industry Co Ltd
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Publication of DK2554677T3 publication Critical patent/DK2554677T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (6)

1. Fremgangsmåde omfattende: måle et levedygtigt celleantal afen mikroorganisme hørende til Bifidobacterium breve alene, blandt bakterier der skal testes der omfatter en mikroorganisme hørende til slægten Bifidobacterium, gennem anvendelse af et dyrkningsmedium, hvor dyrkningsmediet opfylder følgende betingelser 1) og 2), og det levedygtige celleantal bestemmes ved at måle kolonier med en diameter på 0,7 mm eller derover der er dannet i dyrkningsmediet: 1) indeholder mindst én sukkeralkohol valgt fra sorbitol og mannitol som eneste sukkerkilde, hvor en koncentration af sukkeralkohol i dette dyrkningsmedium ikke er større end 4 masseprocent, i forhold til dyrkningsmediets totale masse; og 2) indeholder pepton, kødekstrakt og gærekstrakt som nitrogenkilder, hvor et indhold af pepton er 6,0 til 14,0 g/1000 ml, et indhold af kødekstrakt er 6,0 til 14,0 g/1000 ml, og et indhold af gærekstrakt er 1,8 til 4,2 g/1000 ml, i 1000 ml af dyrkningsmediet.
2. Fremgangsmåden ifølge krav 1, hvor mikroorganismen hørende til Bifidobacterium breve er en Bifidobacterium breve M-16V-stamme.
3. Fremgangsmåden ifølge krav 1 eller 2, hvor bakterien der skal testes omfatter, som mikroorganismen hørende til slægten Bifidobacterium, mikroorganismen hørende til Bifidobacterium breve, og mindst én type mikroorganisme valgt fra gruppen bestående afen mikroorganisme hørende til Bifidobacterium longum subsp. longum, en mikroorganisme hørende til Bifidobacterium animalis subsp. lactis, og en mikroorganisme hørende til Bifidobacterium longum subsp. infantis.
4. Fremgangsmåden ifølge krav 3, hvor mikroorganismen hørende til Bifidobacterium longum subsp. longum er en Bifidobacterium longum subsp. longum BB536-stamme.
5.
Dyrkningsmedium til at måle et levedygtigt celleantal afen mikroorganisme hørende til Bifidobacterium breve alene, blandt bakterier der skal testes der omfatter en mikroorganisme hørende til slægten Bifidobacterium, der anvendes i fremgangsmåden som beskrevet i et hvilket som helst af kravene 1 til 4, hvor dyrkningsmediet opfylder følgende betingelser 1) til 5): 1) indeholder mindst én sukkeralkohol valgt fra sorbitol og mannitol som eneste sukkerkilde;2) en koncentration af denne sukkeralkohol ikke er større end 4 masseprocent, i forhold til dyrkningsmediets totale masse;3) indeholder gærekstrakt, i 1000 ml af dyrkningsmediet, ved en koncentration fra 1,8 til 4,2 g/1000 ml;4) indeholder kødekstrakt, i 1000 ml af dyrkningsmediet, ved en koncentration fra 6,0 til 14,0 g/1000 ml; og5) indeholder pepton, i 1000 ml af dyrkningsmediet, ved en koncentration fra 6,0 til 14,0 g/1000 ml.
DK11759568.6T 2010-03-26 2011-03-25 Levedygtig bakterieantals-målefremgangsmåde og dyrkningsmedium DK2554677T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2010072369 2010-03-26
PCT/JP2011/057335 WO2011118764A1 (ja) 2010-03-26 2011-03-25 生菌数の測定方法及び培地

Publications (1)

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DK2554677T3 true DK2554677T3 (da) 2015-05-18

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DK11759568.6T DK2554677T3 (da) 2010-03-26 2011-03-25 Levedygtig bakterieantals-målefremgangsmåde og dyrkningsmedium

Country Status (7)

Country Link
US (1) US20120244571A1 (da)
EP (1) EP2554677B1 (da)
JP (1) JP5286424B2 (da)
CN (1) CN102510902A (da)
CA (1) CA2781516C (da)
DK (1) DK2554677T3 (da)
WO (1) WO2011118764A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2780707B1 (en) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Method of detecting viable cells in a cell sample
WO2013166336A1 (en) 2012-05-02 2013-11-07 Charles River Laboratories, Inc. Viability staining method
JP6240162B2 (ja) 2012-05-02 2017-11-29 チャールズ リバー ラボラトリーズ， インコーポレイテッド 細胞収集システムおよびその使用
WO2019215873A1 (ja) * 2018-05-10 2019-11-14 森永乳業株式会社 生菌数の測定方法
CN115066490B (zh) * 2020-02-18 2024-04-23 ＡｕＢ株式会社 新型双歧杆菌属细菌以及包含其的组合物

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5321797B2 (ja) 2008-09-18 2013-10-23 住友ベークライト株式会社 光導波路の製造方法

Also Published As

Publication number Publication date
EP2554677B1 (en) 2015-04-29
CN102510902A (zh) 2012-06-20
CA2781516A1 (en) 2011-09-29
EP2554677A1 (en) 2013-02-06
JPWO2011118764A1 (ja) 2013-07-04
US20120244571A1 (en) 2012-09-27
JP5286424B2 (ja) 2013-09-11
EP2554677A4 (en) 2013-10-09
CA2781516C (en) 2016-01-26
WO2011118764A1 (ja) 2011-09-29

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