DK2507618T3 - Fremgangsmåde og system til interaktionsanalyse - Google Patents

Fremgangsmåde og system til interaktionsanalyse Download PDF

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Publication number
DK2507618T3
DK2507618T3 DK10833674.4T DK10833674T DK2507618T3 DK 2507618 T3 DK2507618 T3 DK 2507618T3 DK 10833674 T DK10833674 T DK 10833674T DK 2507618 T3 DK2507618 T3 DK 2507618T3
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DK
Denmark
Prior art keywords
analyte
response
interaction
binding
control
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DK10833674.4T
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English (en)
Inventor
Olof Karlsson
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Ge Healthcare Bio Sciences Ab
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/557—Immunoassay; Biospecific binding assay; Materials therefor using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (9)

1. Fremgangsmåde til at bestemme et eller flere interaktionsparametre for interaktionen mellem en analyt og en ligand ved anvendelse af en biosensor, som omfatter trinnene at: A: at tilvejebringe en sensoroverflade som har liganden immobiliseret derpå, B: at bringe sensoroverfladen i kontakt med en styringsanalyt, C: at registrere sensorresponsen fra binding af styringsanalytten til ligandens bindingssteder, D: at bestemme styremæthedsresponsen (Rmaxc) for interaktionen mellem styringsanalytten og liganden, E: at transformere styremæthedsresponsen (Rmaxc) til en analytmæthedsrespons (RmaxA) ved anvendelse af det relative molære responsbidrag af analytten og styringsanalytten. F: at bringe i kontakt sensoroverfladen med en eller flere prøversom indeholder henholdsvis en koncentration eller forskellige koncentrationer af analytten, G: at registrere sensorresponsen fra binding af analytten til bindingsstederne, og H: at tilpasse det registrerede sensorrespons til en forudbestemt interaktionsmodel ved anvendelse af analytmæthedsresponsen (R max A) og analytkoncentrationen eller koncentrationer for at bestemme interaktionsparametre.
2. Fremgangsmåde ifølge krav 1, hvor styringsanalytten er tilvejebragt med en koncentration således at den er i stand til at optage alle bindingssteder for at tilvejebringe en direkte bestemmelse af styremæthedsresponsen (Rmaxc) fra en interaktionshændelse.
3. Fremgangsmåde ifølge krav 1, hvor styremæthedsresponsen (Rmaxc) bestemmes ud fra en ustabil tilstandsinteraktion mellem styringsanalytten og liganden.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor det relative molære responsbidrag af analytten og styringsanalytten i trin E tilnærmes af molvægtforholdet mellem analytten og styringsanalytten.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, hvor trinnene E til H gentages for en flerhed af analytter.
6. Fremgangsmåde ifølge krav 5, hvor trinnene B til D gentages efter trinnene E til H er blevet udført for et forudbestemt antal analytter.
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 6, omfattende trinnene: I: at udvælge en fremgangsmåde til at bestemme Rmax for hver analyt, fra global bestemmelse af RmaxGiobai fra registreret sensorrespons med forskellige koncentrationer af analytten, og at bestemme RmaxA ved anvendelse af en styringsanalyt og den associerede Rmaxc J: at evaluere bindingsopførslen af analyt-ligand-interaktionen med eller flere bindingsopførselskriterie valgt fra gruppen bestående af: a) . Høj regist reret R b) Forhøjet basislinje efter indsprøjtning c) Forøgende respons under indsprøjtning d) Forhold mellem RmaxGiobai Og RmaxA K: Baseret på evalueringen i trin J at foreslå bindingsmodel som skal anvendes til udregning af interaktionsparameteret af hver analyt.
8. Analytisk system til at detektere molekylære bindingsinteraktioner, omfattende: (i) en biosensor omfattende mindst en sensoroverflade, detektionsorgan til at detektere molekylære bindingsinteraktioner ved den mindst ene sensoroverflade, og organ til at producere detektionsdata som repræsenterer bindingskurver, hvor hver kurve repræsenterer fremgangen af en bindingsinteraktion over tid, og (ii) databehandlingsorgan til at udføre trinnene A til H ifølge krav 1 eller som defineret endvidere i et hvilket som helst af kravene 2 til 7.
9. Computerprogram omfattende programkodeorgan til at bestemme et eller flere interaktionsparametre for interaktionen mellem en analyt og en ligand ifølge et hvilket som helst af kravene 1 til 7 når programmet køres på en computer.
DK10833674.4T 2009-11-30 2010-11-29 Fremgangsmåde og system til interaktionsanalyse DK2507618T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0950913 2009-11-30
SE0951035 2009-12-30
PCT/SE2010/051310 WO2011065912A1 (en) 2009-11-30 2010-11-29 Method and system for interaction analysis

Publications (1)

Publication Number Publication Date
DK2507618T3 true DK2507618T3 (da) 2015-07-27

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Application Number Title Priority Date Filing Date
DK10833674.4T DK2507618T3 (da) 2009-11-30 2010-11-29 Fremgangsmåde og system til interaktionsanalyse

Country Status (6)

Country Link
US (1) US10545090B2 (da)
EP (1) EP2507618B1 (da)
JP (1) JP5683606B2 (da)
CN (1) CN102667448B (da)
DK (1) DK2507618T3 (da)
WO (1) WO2011065912A1 (da)

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US10725030B2 (en) * 2014-11-28 2020-07-28 Ge Healthcare Bio-Sciences Ab Method for determining analyte-ligand binding on a sensor surface
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WO2024041955A2 (en) 2022-08-25 2024-02-29 Cytiva Sweden Ab Sensor surface-based sample analysis

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Also Published As

Publication number Publication date
WO2011065912A1 (en) 2011-06-03
EP2507618A1 (en) 2012-10-10
JP5683606B2 (ja) 2015-03-11
CN102667448A (zh) 2012-09-12
JP2013512457A (ja) 2013-04-11
US20120244637A1 (en) 2012-09-27
CN102667448B (zh) 2015-02-04
US10545090B2 (en) 2020-01-28
EP2507618B1 (en) 2015-05-27
EP2507618A4 (en) 2013-09-04

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