DK2430041T3 - Rekombinante lektiner, som binder til cancerceller, med antitumoraktivitet og klargøringsmetode - Google Patents
Rekombinante lektiner, som binder til cancerceller, med antitumoraktivitet og klargøringsmetode Download PDFInfo
- Publication number
- DK2430041T3 DK2430041T3 DK09840273.8T DK09840273T DK2430041T3 DK 2430041 T3 DK2430041 T3 DK 2430041T3 DK 09840273 T DK09840273 T DK 09840273T DK 2430041 T3 DK2430041 T3 DK 2430041T3
- Authority
- DK
- Denmark
- Prior art keywords
- lectin
- recombinant
- protein
- lectin protein
- nucleic acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Claims (11)
1. Et lektinprotein, der udgøres af en aminosyresekvens af SEQ ID 2 eller 3, som har bedre opløseligheds- og stabilitetsegenskaber sammenlignet med et lektinprotein af SEQ ID 1.
2. Lektinproteinet, som påstået i påstand 1, hvori førnævnte lektin har en molekylærvægt på ca. 16,1 kDa og en Pi-værdi på 6,614.
3. Lektinproteinet som defineret i påstand 1 eller 2 til anvendelse i kræftterapi.
4. Anvendelse af et lektinprotein som defineret i påstand 1 eller 2 til in vz'tro-påvisning afkræftceller.
5. Et nukleinsyremolekyle, der koder for et lektinprotein, som defineret i påstand 1 eller 2, hvori det førnævnte nukleinsyremolekyle er DNA eller RNA.
6. Et nukleinsyremolekyle, som defineret i påstand 5, hvori det førnævnte nukleinsyremolekyle er DNA af SEQ ID 1.1 eller 2.1.
7. En rekombinant vektor; der udgøres af følgende, som er operativt forbundet i 5 ’ til 3 ’ -retningen: En promoter, der virker i en værtscelle; en strukturel nukleinsyresekvens, som defineret i påstand 5 eller 6, hvori den førnævnte nukleinsyresekvens koder for et lektinprotein; og et termineringssignal.
8. En rekombinant vektor, som påstået i påstand 7, hvori førnævnte vektor er i stand til at blive repliceret, transskriberet, translateret og udtrykt i en encellet organisme.
9. En transformeret værtscelle, der indeholder vektoren, som påstået i påstand 7 eller 8.
10. Den transformerede værtscelle, som påstået i påstand 9, hvori førnævnte værtscelle er en Escherichia co/z-bakterie eller en gærcelle.
11. En proces til frembringelse af et rekombinant Sclerotium ro //.s//-lektin protein, der udgøres af: • dyrkning af en værtscelle bestående af den rekombinante vektor, som defineret i påstand 7 eller 8, der koder for lektinproteinet; • udtryk af det rekombinante lektinprotein; og • isolering af førnævnte lektinprotein fra kulturen.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN350MU2009 | 2009-02-18 | ||
| PCT/IN2009/000306 WO2010095143A2 (en) | 2009-02-18 | 2009-05-27 | Cancer cell binding recombinant lectins with antitumor activity and method of preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2430041T3 true DK2430041T3 (da) | 2019-02-25 |
Family
ID=42634281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09840273.8T DK2430041T3 (da) | 2009-02-18 | 2009-05-27 | Rekombinante lektiner, som binder til cancerceller, med antitumoraktivitet og klargøringsmetode |
Country Status (12)
| Country | Link |
|---|---|
| EP (2) | EP3418296A1 (da) |
| CY (1) | CY1123965T1 (da) |
| DK (1) | DK2430041T3 (da) |
| ES (1) | ES2713383T3 (da) |
| HR (1) | HRP20190208T1 (da) |
| HU (1) | HUE041786T2 (da) |
| LT (1) | LT2430041T (da) |
| PL (1) | PL2430041T3 (da) |
| PT (1) | PT2430041T (da) |
| SI (1) | SI2430041T1 (da) |
| TR (1) | TR201901202T4 (da) |
| WO (1) | WO2010095143A2 (da) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014203261A2 (en) * | 2010-02-02 | 2014-12-24 | Unichem Laboratories Ltd. | Recombinant lectin and method of preparing thereof |
| US11965003B2 (en) * | 2018-08-31 | 2024-04-23 | Unichem Laboratories Ltd | Recombinant lectin variants |
| WO2020074977A1 (en) * | 2018-09-07 | 2020-04-16 | Unichem Laboratories Limited | An improved process for the preparation of recombinant lectin protein |
| JP7350843B2 (ja) | 2018-09-16 | 2023-09-26 | ユニケム ラボラトリーズ リミテッド | 炎症性疾患の処置のためのタンパク質 |
| AU2019383003A1 (en) * | 2018-11-19 | 2021-06-03 | Unichem Laboratories Ltd | Combination of chemotherapy with recombinant S. rolfsii lectin |
| US20220339241A1 (en) | 2019-07-09 | 2022-10-27 | Unichem Laboratories Ltd | Stable formulations of recombinant proteins |
| EP4146243A1 (en) | 2020-05-07 | 2023-03-15 | Unichem Laboratories Ltd | Anti-cancer proteins |
| WO2023281432A1 (en) | 2021-07-08 | 2023-01-12 | Unichem Laboratories Ltd | Recombinant proteins, compositions and methods of stabilization thereof |
| WO2023031751A1 (en) | 2021-08-30 | 2023-03-09 | Unichem Laboratories Limited | Protein compositions for the treatment of inflammatory diseases |
| CA3230395A1 (en) * | 2021-10-01 | 2023-04-06 | Unichem Laboratories Limited | Lectin-drug conjugates |
| WO2023111892A1 (en) | 2021-12-16 | 2023-06-22 | Unichem Laboratories Limited | Recombinant protein for treating sars-cov2 disease |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9219905D0 (en) | 1992-09-19 | 1992-11-04 | Univ Liverpool | Lectins |
| US6958321B2 (en) | 2001-05-03 | 2005-10-25 | The Regents Of The University Of Michigan | Isolation, characterization, cloning and use of a mushroom lectin |
-
2009
- 2009-05-27 WO PCT/IN2009/000306 patent/WO2010095143A2/en active Application Filing
- 2009-05-27 PT PT09840273T patent/PT2430041T/pt unknown
- 2009-05-27 PL PL09840273T patent/PL2430041T3/pl unknown
- 2009-05-27 LT LTEP09840273.8T patent/LT2430041T/lt unknown
- 2009-05-27 EP EP18187607.9A patent/EP3418296A1/en active Pending
- 2009-05-27 SI SI200931928T patent/SI2430041T1/sl unknown
- 2009-05-27 DK DK09840273.8T patent/DK2430041T3/da active
- 2009-05-27 TR TR2019/01202T patent/TR201901202T4/tr unknown
- 2009-05-27 EP EP09840273.8A patent/EP2430041B1/en active Active
- 2009-05-27 ES ES09840273T patent/ES2713383T3/es active Active
- 2009-05-27 HU HUE09840273A patent/HUE041786T2/hu unknown
-
2019
- 2019-01-24 CY CY20191100110T patent/CY1123965T1/el unknown
- 2019-01-31 HR HRP20190208TT patent/HRP20190208T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| SI2430041T1 (sl) | 2019-04-30 |
| EP2430041B1 (en) | 2018-10-31 |
| HUE041786T2 (hu) | 2019-06-28 |
| EP2430041A2 (en) | 2012-03-21 |
| PL2430041T3 (pl) | 2019-09-30 |
| CY1123965T1 (el) | 2021-10-29 |
| EP3418296A1 (en) | 2018-12-26 |
| WO2010095143A3 (en) | 2011-01-20 |
| ES2713383T3 (es) | 2019-05-21 |
| LT2430041T (lt) | 2019-02-25 |
| WO2010095143A2 (en) | 2010-08-26 |
| HRP20190208T1 (hr) | 2019-04-05 |
| TR201901202T4 (tr) | 2019-02-21 |
| PT2430041T (pt) | 2019-02-04 |
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