DK2408910T3 - System til chrysosporium lucknowense-proteinfremstilling - Google Patents

System til chrysosporium lucknowense-proteinfremstilling Download PDF

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DK2408910T3
DK2408910T3 DK10709080.5T DK10709080T DK2408910T3 DK 2408910 T3 DK2408910 T3 DK 2408910T3 DK 10709080 T DK10709080 T DK 10709080T DK 2408910 T3 DK2408910 T3 DK 2408910T3
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protein
strain
strains
gene
expression
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Peter J Punt
Christine M Pynnonen
Phillip T Olson
Jan Wery
Johannes Heinrich Visser
Mark A Emalfarb
Jacob Visser
Jan Cornelis Verdoes
Richard Paul Burlingame
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Danisco Us Inc
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Claims (7)

  1. SYSTEM TIL CHRYSOSPORIUM LUCKNOWENSE-PROTEINFREMSTILLING
    1. Svampestamme af Myceliophthora thermophila, oprindeligt anført som Chrysosporium lucknowense, der er WIL indleveret til CBS under accessionsnummer 122189, eller WlL#100.1, der er indleveret til CBS under accessionsnummer 122190.
  2. 2. Stamme ifølge krav 1, der endvidere er kendetegnet ved, at genet, der koder for endochitinase 1 (chil), er blevet brudt.
  3. 3. Stamme ifølge krav 1 eller 2, endvidere kendetegnet ved, at ét eller flere gener, der er udvalgt fra gruppen bestående af de, der koder for alkalisk protease 1 (alpi), alkalisk protease 2 (alp2), proteinase A (pep4), glycoamylase (Glal), exo-chitinase (Chi2) og laminarinase (Lami), er blevet brudt.
  4. 4. Stamme ifølge krav 3, der er W1L# 100.1 Aalp 1Δρ\τ5 eller W1L# 100.1 Aalp lAclii 1Δρ\τ5.
  5. 5. Fremgangsmåde til homolog og/eller heterolog fremstilling af et rent protein med en renhed, der er højere end 75 %, hvilken fremgangsmåde omfatter ekspression af et gen, der koder for proteinet i stammen ifølge et hvilket som helst af kravene 1 til 4.
  6. 6. Fremgangsmåde til fremstilling af kunstige proteinblandinger, hvilken fremgangsmåde omfatter ekspression af gener, der koder for hvert af proteinerne i en stamme ifølge et hvilket som helst af kravene 1 til 4.
  7. 7. Fremgangsmåde til forenklet screening af stammer, der funktionelt udtrykker et ønsket enzym ved anvendelse af stammer som defineret i et hvilket som helst af kravene 1 til 4.
DK10709080.5T 2009-03-16 2010-03-16 System til chrysosporium lucknowense-proteinfremstilling DK2408910T3 (da)

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EP09003750 2009-03-16
PCT/NL2010/000045 WO2010107303A2 (en) 2009-03-16 2010-03-16 New fungal production system

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US (2) US9175296B2 (da)
EP (2) EP2408910B1 (da)
CN (1) CN102421895B (da)
BR (1) BRPI1009488B1 (da)
CA (1) CA2755417C (da)
DK (2) DK3421597T3 (da)
ES (2) ES2678450T3 (da)
IL (2) IL215116A (da)
MX (1) MX2011009636A (da)
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RU2016104064A (ru) 2013-07-10 2017-08-15 Новартис Аг Клетки мицелиальных грибов с множественной недостаточностью протеаз и способы их использования
US10513724B2 (en) 2014-07-21 2019-12-24 Glykos Finland Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
WO2016142536A1 (en) 2015-03-11 2016-09-15 Genencor International B.V. Enzymatic activity of lytic polysaccharide monooxygenase
DK3313999T3 (da) 2015-06-24 2021-08-02 Genencor Int Bv Polypeptider med demethyleringsaktivitet
KR20180081817A (ko) * 2015-12-02 2018-07-17 바스프 에스이 감소된 clr1 활성을 갖는 사상 진균에서 단백질을 생산하는 방법
CA3004990A1 (en) * 2015-12-02 2017-06-08 Basf Se Method of producing proteins in filamentous fungi with decreased clr2 activity
WO2017147163A1 (en) 2016-02-22 2017-08-31 Danisco Us Inc. Fungal high-level protein production system
ES2647322B1 (es) * 2016-05-20 2018-10-05 Abengoa Bioenergía Nuevas Tecnologías, S.A. Célula hospedadora Myceliophthora thermophila con actividad celulolítica mejorada y composiciones enzimáticas obtenidas por la misma
US11738080B2 (en) 2017-08-21 2023-08-29 Dyadic International Inc. Production of flu vaccine in Myceliophthora thermophila
WO2019074828A1 (en) 2017-10-09 2019-04-18 Danisco Us Inc CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE

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