DK2362915T3 - Crispr-sekvenser fra bifidobakterier - Google Patents
Crispr-sekvenser fra bifidobakterier Download PDFInfo
- Publication number
- DK2362915T3 DK2362915T3 DK09799777.9T DK09799777T DK2362915T3 DK 2362915 T3 DK2362915 T3 DK 2362915T3 DK 09799777 T DK09799777 T DK 09799777T DK 2362915 T3 DK2362915 T3 DK 2362915T3
- Authority
- DK
- Denmark
- Prior art keywords
- sequence
- seq
- crispr
- balal
- spacer
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (11)
1. Isoleret nukleinsyre, der omfatter en sekvens af et CRISPR-locus fra bifidobakterier udvalgt fra gruppen, der består af a) Balal (SEQ ID NO: 1); b) en Balal- gentagelsessekvens udvalgt blandt SEQ ID NO: 2 og sekvensvarianter deraf; c) en Balal spacersekvens udvalgt blandt SEQ ID NO: 3-24; hvor sekvensvarianterne af SEQ ID NO: 2 omfatter en eller flere substitutioner udvalgt fra gruppen, der består af: anvendelse af C i stedet for T i position 12, anvendelse af C i stedet for T i position 14 og anvendelse af G i stedet for A i position 36.
2. Isoleret nukleinsyre ifølge krav 1, hvor sekvensvarianterne af SEQ ID NO: 2 har anvendelse af C i stedet for T i position 12, anvendelse af C i stedet for T i position 14 og anvendelse af G i stedet for A i position 36.
3. Isoleret nukleinsyre ifølge krav 1, hvor sekvensen er udvalgt blandt SEQ ID NO: 13, 14 og 15.
4. Isoleret nukleinsyre, der omfatter en Balal-gentagelsessekvens af SEQ ID NO: 2 og en Balal-spacersekvens udvalgt blandt SEQ ID NO: 3-24.
5. Vektor, der omfatter et CRISPR-locus udvalgt fra gruppen, der består af a) Balal (SEQ ID NO: 1); b) en Balal- gentagelsessekvens udvalgt blandt SEQ ID NO: 2 og sekvensvarianter deraf; og c) en Balal-spacersekvens udvalgt blandt SEQ ID NO: 3-24; hvor sekvensvarianterne af SEQ ID NO: 2 omfatter en eller flere substitutioner udvalgt fra gruppen, der består af: anvendelse af C i stedet for T i position 12, anvendelse af C i stedet for T i position 14 og anvendelse af G i stedet for A i position 36.
6. Vektor, der omfatter en Balal-gentagelse-spacer- enhedssekvens, hvor gentagelsessekvensen er udvalgt blandt SEQ ID NO: 2 og varianter deraf, og spacersekvensen er udvalgt blandt SEQ ID NO: 3-24, hvor sekvensvarianterne af SEQ ID NO: 2 omfatter en eller flere substitutioner udvalgt fra gruppen, der består af: anvendelse af C i stedet for T i position 12, anvendelse af C i stedet for T i position 14 og anvendelse af G i stedet for A i position 36.
7. Rekombinant stamme af bakterier med ændret fagresistens, der omfatter a) en Balal-gentagelsessekvens udvalgt blandt SEQ ID NO: 2 og varianter deraf; og b) en Balal-spacersekvens udvalgt blandt SEQ ID NO: 3-24; hvor sekvensvarianterne af SEQ ID NO: 2 omfatter en eller flere substitutioner udvalgt fra gruppen, der består af: anvendelse af C i stedet for T i position 12, anvendelse af C i stedet for T i position 14 og anvendelse af G i stedet for A i position 36.
8. Rekombinant stamme af bakterier med ændret fagresistens, der omfatter en Balal-gentagelse-spacer-enhedssekvens, hvor gentagelsessekvensen er udvalgt blandt SEQ ID NO: 2 og varianter deraf, og spacersekvensen er udvalgt blandt SEQ ID NO: 3-24, hvor sekvensvarianterne af SEQ ID NO: 2 omfatter en eller flere substitutioner udvalgt fra gruppen, der består af: anvendelse af C i stedet for T i position 12, anvendelse af C i stedet for T i position 14 og anvendelse af G i stedet for A i position 36.
9. Fremgangsmåde til fremstilling af en rekombinant bakteriestamme med ændret fagresistens, der omfatter: (a) transformering af bakterier med en nukleinsyre, der omfatter en Balal-spacersekvens udvalgt blandt SEQ ID NO: 3-24; (b) etablering af kontakt mellem de transformerede bakterier og en fag; og (c) isolering af transformerede bakterier, der fremviser ændret resistens over for fagen.
10. Fremgangsmåde til stammetypebestemmelse, der omfatter: (a) amplificering af genomisk DNA fra en stamme af interesse ved anvendelse af mindst ét primerpar, hvor det genomiske DNA omfatter mindst en del af en sekvens af et CRISPR-locus, hvor CRISPR-locuset er Balal (SEQ ID NO: 1); (b) påvisning af et amplikon dannet i trin (a) , hvor det påviste amplikon indikerer stammetypen.
11. Stammetypebestemmelseskit, der omfatter: (a) en beholder med en amplifikationsblanding, der omfatter en DNA-polymerase, en amplifikationsbuffer og mindst ét primerpar, hvor hver primer af parret er komplementær til en del af genomisk DNA, således at primerparret amplificerer mindst en del af en gentagelses- eller spacersekvens af et CRISPR-locus Balal (SEQ ID NO: 1); og (b) en beholder med en påvisningsblanding, der omfatter en probe, der er i stand til under stringente betingelser at hybridisere til mindst en del af CRISPR-locuset, der amplificeres ved hjælp af primerparret.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11267308P | 2008-11-07 | 2008-11-07 | |
| PCT/US2009/063497 WO2010054154A2 (en) | 2008-11-07 | 2009-11-06 | Bifidobacteria crispr sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2362915T3 true DK2362915T3 (da) | 2017-03-27 |
Family
ID=42025708
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09799777.9T DK2362915T3 (da) | 2008-11-07 | 2009-11-06 | Crispr-sekvenser fra bifidobakterier |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10662227B2 (da) |
| EP (1) | EP2362915B1 (da) |
| DK (1) | DK2362915T3 (da) |
| MX (1) | MX337838B (da) |
| RU (1) | RU2570562C2 (da) |
| WO (1) | WO2010054154A2 (da) |
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| US10066233B2 (en) | 2005-08-26 | 2018-09-04 | Dupont Nutrition Biosciences Aps | Method of modulating cell resistance |
| BR112012028805A2 (pt) | 2010-05-10 | 2019-09-24 | The Regents Of The Univ Of California E Nereus Pharmaceuticals Inc | composições de endorribonuclease e métodos de uso das mesmas. |
| DK2630156T3 (da) | 2010-10-20 | 2018-12-17 | Dupont Nutrition Biosci Aps | Crispr-cas sekvenser af lactococcus |
| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| DK2800811T3 (da) | 2012-05-25 | 2017-07-17 | Univ Vienna | Fremgangsmåder og sammensætninger til rna-rettet target-dna-modificering og til rna-rettet modulering af transkription |
| KR102243092B1 (ko) | 2012-12-06 | 2021-04-22 | 시그마-알드리치 컴퍼니., 엘엘씨 | Crispr-기초된 유전체 변형과 조절 |
| EP2971167B1 (en) | 2013-03-14 | 2019-07-31 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| IL289396B2 (en) | 2013-03-15 | 2023-12-01 | The General Hospital Coporation | Using tru-grnas to increase the specificity of RNA-guided genome editing |
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| WO2014165825A2 (en) | 2013-04-04 | 2014-10-09 | President And Fellows Of Harvard College | Therapeutic uses of genome editing with crispr/cas systems |
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| CA2944978C (en) * | 2014-04-08 | 2024-02-13 | North Carolina State University | Methods and compositions for rna-directed repression of transcription using crispr-associated genes |
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| JP7051438B2 (ja) | 2015-06-15 | 2022-04-11 | ノース カロライナ ステート ユニバーシティ | 核酸およびｒｎａに基づく抗菌剤の効率的な送達のための方法および組成物 |
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| EP3356533A1 (en) | 2015-09-28 | 2018-08-08 | North Carolina State University | Methods and compositions for sequence specific antimicrobials |
| IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and their uses |
| WO2017112620A1 (en) | 2015-12-22 | 2017-06-29 | North Carolina State University | Methods and compositions for delivery of crispr based antimicrobials |
| SG11201806134SA (en) | 2016-01-27 | 2018-08-30 | Oncorus Inc | Oncolytic viral vectors and uses thereof |
| EP3219799A1 (en) | 2016-03-17 | 2017-09-20 | IMBA-Institut für Molekulare Biotechnologie GmbH | Conditional crispr sgrna expression |
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| IL308426A (en) | 2016-08-03 | 2024-01-01 | Harvard College | Adenosine nuclear base editors and their uses |
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| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
| EP3861120A4 (en) | 2018-10-01 | 2023-08-16 | North Carolina State University | RECOMBINANT TYPE I CRISPR-CAS SYSTEM |
| EP3921417A4 (en) | 2019-02-04 | 2022-11-09 | The General Hospital Corporation | ADENINE DNA BASE EDITOR VARIANTS WITH REDUCED OFF-TARGET RNA EDITING |
| WO2020191243A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| US20230279442A1 (en) | 2021-12-15 | 2023-09-07 | Versitech Limited | Engineered cas9-nucleases and method of use thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
| EP0769068B1 (en) * | 1994-06-24 | 2005-10-12 | Innogenetics N.V. | Simultaneous detection, identification and differentiation of eubacterial taxa using a hybridization assay |
| US7919277B2 (en) * | 2004-04-28 | 2011-04-05 | Danisco A/S | Detection and typing of bacterial strains |
| KR100625090B1 (ko) | 2005-03-02 | 2006-09-20 | 모딘코리아 유한회사 | 고 마그네슘 알루미늄 합금용 접합시트 및 고 마그네슘 알루미늄 합금 접합방법 |
| US10066233B2 (en) | 2005-08-26 | 2018-09-04 | Dupont Nutrition Biosciences Aps | Method of modulating cell resistance |
| ES2590925T3 (es) | 2006-05-19 | 2016-11-24 | Dupont Nutrition Biosciences Aps | Microorganismos marcados y métodos de marcado |
-
2009
- 2009-11-06 RU RU2011122776/10A patent/RU2570562C2/ru active
- 2009-11-06 EP EP09799777.9A patent/EP2362915B1/en active Active
- 2009-11-06 WO PCT/US2009/063497 patent/WO2010054154A2/en active Application Filing
- 2009-11-06 MX MX2011004854A patent/MX337838B/es active IP Right Grant
- 2009-11-06 DK DK09799777.9T patent/DK2362915T3/da active
- 2009-11-06 US US13/127,934 patent/US10662227B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20110300538A1 (en) | 2011-12-08 |
| EP2362915B1 (en) | 2016-12-21 |
| WO2010054154A3 (en) | 2010-09-16 |
| MX2011004854A (es) | 2011-10-28 |
| MX337838B (es) | 2016-03-22 |
| US10662227B2 (en) | 2020-05-26 |
| RU2570562C2 (ru) | 2015-12-10 |
| RU2011122776A (ru) | 2012-12-20 |
| EP2362915A2 (en) | 2011-09-07 |
| WO2010054154A2 (en) | 2010-05-14 |
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