DK2357227T3 - Optimerede cellulaseenzymer - Google Patents
Optimerede cellulaseenzymer Download PDFInfo
- Publication number
- DK2357227T3 DK2357227T3 DK10153355.2T DK10153355T DK2357227T3 DK 2357227 T3 DK2357227 T3 DK 2357227T3 DK 10153355 T DK10153355 T DK 10153355T DK 2357227 T3 DK2357227 T3 DK 2357227T3
- Authority
- DK
- Denmark
- Prior art keywords
- gly
- thr
- ser
- asp
- asn
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Claims (14)
1. Polypeptid med cellobiohydrolaseaktivitet, hvor polypeptidet omfatter en aminosyre-sekvens, der har mindst 85% sekvensidentitet med SEQ ID NO: 2, idet aminosyreresten i stilling Q1 i SEQ ID NO: 2 er modificeret ved erstatning eller udeladelse.
2. Polypeptid ifølge krav 1, hvor polypeptidet opretholder 50% af sin maksimale substratomdannelseskapacitet, når omdannelsen foretages i 60 minutter ved en temperatur på 60°C eller derover.
3. Polypeptid ifølge krav 1 eller 2, hvor polypeptidet omfatter en aminosyresekvens, der har mindst 90%, fortrinsvis mindst 95% og især mindst 99% sekvensidentitet med SEQ ID NO: 2.
4. Polypeptid ifølge et eller flere af de foregående krav, hvor polypeptidets aminosyresekvens har den ved SEQ ID NO: 2 definerede sekvens eller en sekvens, som defineret ved SEQ ID NO: 2, hvori 1 til 75 aminosyrerester, fortrinsvis 1 til 35 aminosyrerester, er erstattet, udeladt eller indsat.
5. Polypeptid ifølge krav 4, hvori yderligere én eller flere af følgende aminosyrerester i den ved SEQ ID NO: 2 definerede sekvens er modificeret ved erstatning eller udeladelse: stillingerne G4, A6, T15, Q28, W40, D64, E65, A72, S86, K92, V130, V152, Y155, K159, D181, E183, N194, D202, P224, T243, Y244, I277, K304, N310, S311, N318, D320, T335, T344, D346, Q349, A358, Y374, A375, T392, T393, D410, Y422, P442, N445, R446, T456, S460, P462, G463, H468 og/eller V482 blandt aminosyrerne 1 til 500 i SEQ ID NO: 2.
6. Polypeptid ifølge krav 5, hvor polypeptidet omfatter én eller flere af følgende foretrukne udvekslinger i forhold til den ved SEQ ID NO: 2 definerede sekvens:
7. Polypeptid ifølge et eller flere af de foregående krav, hvor polypeptidet har en aminosyresekvens valgt blandt listen over følgende mutationer i SEQ ID NO: 2:
8. Polypeptid ifølge et eller flere af de foregående krav, som udtrykkes og udskilles i et niveau på mere end 100 mg/l, fortrinsvis mere end 200 mg/l, særlig foretrukket mere end 500 mg/l og især mere end 1 g/l til centrifugatet efter indføring af en nukleinsyre, der koder for et polypeptid, som har en aminosyresekvens med mindst 85% sekvensidentitet med SEQ ID NO: 2, i en gær, hvori aminosyreresten i stilling Q1 i SEQ ID NO: 2 må være modificeret ved erstatning eller udeladelse.
9. Nukleinsyre, der koder for polypeptidet ifølge et eller flere af kravene 1 til 8, fortrinsvis med mindst 95% identitet med SEQ ID NO: 1.
10. Vektor, der omfatter nukleinsyren ifølge krav 9.
11. Værtscelle transformeret med en vektor ifølge krav 10.
12. Værtscelle ifølge krav 11, hvor værtscellen er afledt af gruppen, der består af Saccharomyces, Schizosaccharomyces, Kluyveromyces, Pichia, Pichia, Hansenula, Aspergillus, Trichoderma, Penicillium, Candida og Yarrowina.
13. Sammensætning omfattende polypeptidet ifølge et eller flere af kravene 1 til 9 og én eller flere endoglucanaser og/eller én eller flere β-glucosidaser og/eller én eller flere andre cellobiohydralaser og/eller én eller flere xylanaser.
14. Anvendelse af polypeptidet ifølge et eller flere af kravene 1 til 9 eller af sammensætningen ifølge krav 13 til enzymatisk nedbrydning af lignocellulosisk biomasse og/eller til tekstilforarbejdning og/eller som bestanddel i vaskemidler og/eller som bestanddel i levnedsmiddel- eller fodersammensætninger.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10153355.2A EP2357227B1 (en) | 2010-02-11 | 2010-02-11 | Optimized cellulase enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2357227T3 true DK2357227T3 (da) | 2015-09-14 |
Family
ID=42027636
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10153355.2T DK2357227T3 (da) | 2010-02-11 | 2010-02-11 | Optimerede cellulaseenzymer |
| DK11706777.7T DK2534245T3 (da) | 2010-02-11 | 2011-02-11 | Optimerede cellulaseenzymer |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11706777.7T DK2534245T3 (da) | 2010-02-11 | 2011-02-11 | Optimerede cellulaseenzymer |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9458443B2 (da) |
| EP (2) | EP2357227B1 (da) |
| CN (1) | CN102844430A (da) |
| AU (1) | AU2011214298B2 (da) |
| BR (1) | BR112012020277A2 (da) |
| CA (1) | CA2789423C (da) |
| DK (2) | DK2357227T3 (da) |
| ES (2) | ES2549328T3 (da) |
| HR (2) | HRP20151043T1 (da) |
| MX (1) | MX344963B (da) |
| PL (2) | PL2357227T3 (da) |
| RS (1) | RS57695B1 (da) |
| SI (2) | SI2357227T1 (da) |
| WO (1) | WO2011098551A2 (da) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2796189A1 (en) | 2010-06-01 | 2011-12-08 | California Institute Of Technology | Stable, functional chimeric cellobiohydrolase class i enzymes |
| CA2811789A1 (en) | 2010-10-06 | 2012-04-12 | Bp Corporation North America Inc. | Variant cbh i polypeptides with reduced product inhibition |
| US20140080182A1 (en) * | 2011-03-31 | 2014-03-20 | Novozymes, Inc. | Cellulose Binding Domain Variants and Polynucleotides Encoding Same |
| CA2856083A1 (en) * | 2011-11-15 | 2013-05-23 | Novozymes, Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| WO2013091577A1 (en) | 2011-12-22 | 2013-06-27 | Novozymes, Inc. | Hybrid polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| MX2015007234A (es) | 2012-12-12 | 2015-10-29 | Danisco Us Inc | Variantes de celobiohidrolasas. |
| US20150315560A1 (en) * | 2012-12-12 | 2015-11-05 | Danisco Us Inc. | Variants of cellobiohydrolases |
| CN105164254B (zh) | 2013-03-08 | 2019-06-28 | 诺维信公司 | 纤维二糖水解酶变体和编码它们的多核苷酸 |
| CN107002056A (zh) | 2014-09-05 | 2017-08-01 | 诺维信公司 | 碳水化合物结合模块变体以及编码它们的多核苷酸 |
| DK3191587T3 (da) | 2014-09-08 | 2019-10-14 | Novozymes As | Cellobiohydrolasevarianter og polynukleotider, som koder for dem |
| JP6378622B2 (ja) * | 2014-12-04 | 2018-08-22 | 本田技研工業株式会社 | 高活性セロビオヒドロラーゼ |
| US10557127B2 (en) | 2015-02-24 | 2020-02-11 | Novozymes A/S | Cellobiohydrolase variants and polynucleotides encoding same |
| WO2017177289A1 (en) | 2016-04-15 | 2017-10-19 | Vtt International Oy | Process for conversion of biomass into fermentable sugars with integrated enzyme |
| CN109715182A (zh) * | 2016-06-15 | 2019-05-03 | 科德克希思公司 | 工程化β-葡糖苷酶和葡糖基化方法 |
| CN109337888B (zh) * | 2018-11-12 | 2021-08-17 | 南开大学 | 纤维二糖水解酶突变体及其生产方法和应用 |
| CN110373402B (zh) * | 2019-08-13 | 2021-07-20 | 山东农业大学 | 一种提升耐热纤维素酶酶活力和热稳定性的方法 |
| CN113186175B (zh) * | 2021-06-04 | 2023-08-04 | 翌圣生物科技(上海)股份有限公司 | 突变型Taq DNA聚合酶、编码DNA序列、重组载体、重组表达细胞及其应用 |
| CN114426961B (zh) * | 2022-03-10 | 2024-01-26 | 安徽大学 | 一种β-葡萄糖苷酶突变体、其编码基因、表达菌株及其应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK115890D0 (da) | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
| AU6210800A (en) | 1999-07-13 | 2001-01-30 | Midwest Research Institute | Cellobiohydrolase reduced glycosylation variants: cbhin45a; cbhin270a; and cbhin384a |
| US8637293B2 (en) | 1999-07-13 | 2014-01-28 | Alliance For Sustainable Energy, Llc | Cellobiohydrolase I enzymes |
| US7375197B2 (en) * | 2002-01-14 | 2008-05-20 | Midwest Research Institute | Cellobiohydrolase I gene and improved variants |
| EP2298868B1 (en) | 2001-06-26 | 2015-01-07 | Novozymes A/S | Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same |
| ES2375054T3 (es) | 2002-08-16 | 2012-02-24 | Danisco Us Inc. | Nueva variante de celulasa cbh1 de hypocrea jecorina. |
| WO2004078919A2 (en) * | 2003-02-27 | 2004-09-16 | Midwest Research Institute | Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum |
| EP2311942A1 (en) | 2003-03-21 | 2011-04-20 | Genecor International, Inc. | Novel cbh1 homologs and variant cbh1 cellulases |
| DK1627050T3 (da) | 2003-04-01 | 2013-10-14 | Danisco Us Inc | Humicola grisea cbh1.1-variant |
| EP2617825B1 (en) | 2003-08-25 | 2015-04-01 | Novozymes, Inc. | Variants of glycoside hydrolases |
| PT1874927E (pt) * | 2005-04-29 | 2014-04-23 | Ab Enzymes Oy | Celulases melhoradas |
| CN1757710A (zh) | 2005-07-15 | 2006-04-12 | 山东农业大学 | 表达cbh1基因的酵母工程菌株及其构建方法 |
| FI120045B (fi) | 2005-12-22 | 2009-06-15 | Roal Oy | Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit |
| US8017373B2 (en) * | 2006-08-31 | 2011-09-13 | Iogen Energy Corporation | Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks |
| US10676751B2 (en) * | 2008-02-29 | 2020-06-09 | The Trustees Of The University Of Pennsylvania | Production and use of plant degrading materials |
| BRPI0911966B1 (pt) * | 2008-05-11 | 2018-11-13 | Univ Stellenbosch | polinucleotídeo codificando uma celobioidrolase |
| US9994835B2 (en) * | 2008-05-11 | 2018-06-12 | Lallemand Hungary Liquidity Management Llc | Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases |
| US8470592B2 (en) * | 2008-07-07 | 2013-06-25 | Mascoma Corporation | Isolation and characterization of Schizochytrium aggregatum cellobiohydrolase I (Cbh 1) |
| DK2421965T3 (da) * | 2009-04-24 | 2016-09-05 | Dsm Ip Assets Bv | Carbohydratdegraderende polypeptid og anvendelser deraf |
-
2010
- 2010-02-11 EP EP10153355.2A patent/EP2357227B1/en active Active
- 2010-02-11 ES ES10153355.2T patent/ES2549328T3/es active Active
- 2010-02-11 SI SI201031027T patent/SI2357227T1/sl unknown
- 2010-02-11 PL PL10153355T patent/PL2357227T3/pl unknown
- 2010-02-11 DK DK10153355.2T patent/DK2357227T3/da active
-
2011
- 2011-02-11 WO PCT/EP2011/052023 patent/WO2011098551A2/en active Application Filing
- 2011-02-11 CN CN2011800185464A patent/CN102844430A/zh active Pending
- 2011-02-11 US US13/578,291 patent/US9458443B2/en active Active
- 2011-02-11 PL PL11706777T patent/PL2534245T3/pl unknown
- 2011-02-11 SI SI201131575T patent/SI2534245T1/sl unknown
- 2011-02-11 CA CA2789423A patent/CA2789423C/en active Active
- 2011-02-11 ES ES11706777.7T patent/ES2689939T3/es active Active
- 2011-02-11 AU AU2011214298A patent/AU2011214298B2/en active Active
- 2011-02-11 EP EP11706777.7A patent/EP2534245B1/en active Active
- 2011-02-11 DK DK11706777.7T patent/DK2534245T3/da active
- 2011-02-11 BR BR112012020277A patent/BR112012020277A2/pt not_active IP Right Cessation
- 2011-02-11 RS RS20181132A patent/RS57695B1/sr unknown
- 2011-02-11 MX MX2012009357A patent/MX344963B/es active IP Right Grant
-
2015
- 2015-10-01 HR HRP20151043TT patent/HRP20151043T1/hr unknown
-
2018
- 2018-10-03 HR HRP20181587TT patent/HRP20181587T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011098551A2 (en) | 2011-08-18 |
| CA2789423C (en) | 2017-12-12 |
| US20130123115A1 (en) | 2013-05-16 |
| EP2534245B1 (en) | 2018-07-04 |
| HRP20181587T1 (hr) | 2018-12-14 |
| AU2011214298B2 (en) | 2014-11-06 |
| CA2789423A1 (en) | 2011-08-18 |
| HRP20151043T1 (hr) | 2015-11-06 |
| PL2357227T3 (pl) | 2015-12-31 |
| EP2357227B1 (en) | 2015-07-15 |
| ES2549328T3 (es) | 2015-10-27 |
| DK2534245T3 (da) | 2018-10-01 |
| PL2534245T3 (pl) | 2019-02-28 |
| ES2689939T3 (es) | 2018-11-16 |
| US9458443B2 (en) | 2016-10-04 |
| CN102844430A (zh) | 2012-12-26 |
| WO2011098551A3 (en) | 2012-05-31 |
| BR112012020277A2 (pt) | 2015-09-15 |
| AU2011214298A1 (en) | 2012-09-06 |
| EP2357227A1 (en) | 2011-08-17 |
| SI2357227T1 (sl) | 2015-11-30 |
| RS57695B1 (sr) | 2018-11-30 |
| SI2534245T1 (sl) | 2019-01-31 |
| EP2534245A2 (en) | 2012-12-19 |
| MX344963B (es) | 2017-01-11 |
| MX2012009357A (es) | 2012-12-17 |
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