DK2346914T3 - Polymerpartikler, som er fremstillet af polymeriserbare alkylenglycol-(meth)acrylat-monomerer - Google Patents

Polymerpartikler, som er fremstillet af polymeriserbare alkylenglycol-(meth)acrylat-monomerer Download PDF

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DK2346914T3
DK2346914T3 DK09748135.2T DK09748135T DK2346914T3 DK 2346914 T3 DK2346914 T3 DK 2346914T3 DK 09748135 T DK09748135 T DK 09748135T DK 2346914 T3 DK2346914 T3 DK 2346914T3
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polymer
poly
methacrylate
particles
monomers
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DK09748135.2T
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English (en)
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Cameron Alexander
Kevin Shakesheff
Liang He
Martyn Davies
Wenxin Wang
Brian Saunders
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Univ Nottingham
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/10—Esters
    • C08F220/26—Esters containing oxygen in addition to the carboxy oxygen
    • C08F220/28—Esters containing oxygen in addition to the carboxy oxygen containing no aromatic rings in the alcohol moiety
    • C08F220/285—Esters containing oxygen in addition to the carboxy oxygen containing no aromatic rings in the alcohol moiety and containing a polyether chain in the alcohol moiety
    • C08F220/286—Esters containing oxygen in addition to the carboxy oxygen containing no aromatic rings in the alcohol moiety and containing a polyether chain in the alcohol moiety and containing polyethylene oxide in the alcohol moiety, e.g. methoxy polyethylene glycol (meth)acrylate
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/10—Esters
    • C08F220/26—Esters containing oxygen in addition to the carboxy oxygen
    • C08F220/28—Esters containing oxygen in addition to the carboxy oxygen containing no aromatic rings in the alcohol moiety
    • C08F220/285—Esters containing oxygen in addition to the carboxy oxygen containing no aromatic rings in the alcohol moiety and containing a polyether chain in the alcohol moiety
    • C08F220/287—Esters containing oxygen in addition to the carboxy oxygen containing no aromatic rings in the alcohol moiety and containing a polyether chain in the alcohol moiety and containing polypropylene oxide in the alcohol moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Preparation (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Claims (14)

1. Fremgangsmåde til fremstilling af polymerpartikler, hvilken fremgangsmåde omfatter: - tilvejebringelse af én eller flere polymerer, som er en polymer, der udviser en lavere kritisk opløsningstemperatur i vandige medier, som er mellem 10 og 90°C, idet polymeren er polymerisationsproduktet af én eller flere monomerer, der er valgt blandt polymeriserbare alkylenglycolacrylat-monomerer og polyme-riserbare alkylenglycolmethacrylat-monomerer; og - tilsætning af polymeren som en overfladeændrende surfaktant til en reaktionsblanding til fremstilling af en polymerpartikel ved en fremgangsmåde, som er valgt blandt emulgeringsfremgangsmåder, diffusionsfremgangsmåder og fordampningsfremgangsmåder.
2. Polymerpartikler, hvor partiklerne er stabile polymerpartikler, som har en størrelse på mellem 500 nm og 100 μηι i diameter, målt ved dynamisk lysspredning, og hvor partiklerne: (a) er dannet ved en fremgangsmåde, som involverer anvendelse af en LCST-polymer under emulgerings/diffusions/fordampningssynteseprocedurer med po-ly(mælke-co-glycolsyre), idet LCST-polymeren er en polymer, der udviser en lavere kritisk opløsningstemperatur i vandige medier, som er mellem 10 og 90°C, idet denne polymer er polymerisationsproduktet af én eller flere monomerer, der er valgt blandt polymeriserbare alkylenglycolacrylat-monomerer og polymeriserbare alkylenglycolmethacrylat-monomerer; eller (b) har en bionedbrydelig polymerkerne, som er poly(mælke-co-glycolsyre), og en termoresponsiv biokompatibel copolymerkorona, hvor copolymeren er en polymer, der udviser en lavere kritisk opløsningstemperatur i vandige medier, som er mellem 10 og 90°C, idet denne polymer er polymerisationsproduktet af én eller flere monomerer, der er valgt blandt polymeriserbare alkylenglycolacrylat-monomerer og polymeriserbare alkylenglycolmethacrylat-monomerer.
3. Partikler ifølge krav 2, hvor polymerpartiklerne: (a) har en bulkfase eller kerne af bionedbrydelige polymerer, som er valgt blandt poly(mælke-co-glycolsyre), poly(mælkesyre) og poly(glycolsyre); og/eller (b) i deres bulkfase eller kerne omfatter aktive bestanddele, som er valgt blandt: smagsstoffer, duftstoffer, små molekylelægemidler og biofarmaceutika og vækstfaktorer med langsom afgivelse og andre opløselige molekyler til vævsvækst, organ reparation og regenerativ medicin.
4. Partikler ifølge krav 2(a), hvor polymerpartiklerne er stabile polymerpartikler, som har en størrelse på mellem 500 nm og 5 pm i diameter.
5. Partikler ifølge et hvilket som helst af kravene 2 til 4, hvor de polymeriserbare alky-lenglycolacrylat-monomerer og polymeriserbare alkylenglycolmethacrylat-monomerer er valgt blandt: di(ethylenglycol)-acrylat, oligo(ethylenglycol)-acrylat, poly(ethylen-glycol)-acrylat, di(ethylenglycol)-methacrylat, oligo(ethylenglycol)-methacrylat, poly-(ethylenglycol)-methacrylat, poly(propylenglycol)-acrylat og poly(propylenglycol)-methacrylat.
6. Partikler ifølge et hvilket som helst af kravene 2 til 5, hvor den polymer, der udviser en lavere kritisk opløsningstemperatur: (a) er polymerisationsproduktet af to monomerer eller (b) er polymerisationsproduktet af poly(ethylenglycol)methacrylat (PEGMA) og po-ly(propylenglycol)methacrylat (PPGMA) eller (c) er en polymer, som har en molær masse på 25-75 kDa med et polydispersitets-(Mw/Mn)-indeks på 1-2,5, målt ved GPC.
7. Anvendelse af polymerpartikler som defineret i et hvilket som helst af kravene 2 til 6 (a) til anvendelser med kontrolleret afgivelse, hvor anvendelserne eventuelt er valgt blandt anvendelser med smagsafgivelse, anvendelser med duftafgivelse og biomedicinske anvendelser, og hvor anvendelserne eventuelt kan involvere afgivelse af små molekylelægemidler eller biofarmaceutika til konventionel lægemiddelafgivelse eller afgivelse af vækstfaktorer med langsom afgivelse og andre opløselige molekyler til vævsvækst, organreparation og regenerativ medicin; eller (b) som celleunderstøtningsmatrixer, hvor polymerpartiklerne eventuelt understøtter cellevækst.
8. Produkt, som omfatter polymerpartikler som defineret i et hvilket som helst af kravene 2 til 6 og biologisk materiale, hvor det biologiske materiale eventuelt er celler.
9. Celleunderstøtningsmatrix, som omfatter polymerpartikler som defineret i et hvilket som helst af kravene 2 til 6, hvor polymerpartiklerne eventuelt understøtter cellevækst.
10. Fritflydende suspension, som omfatter (i) celler og (ii) polymerpartikler som defineret i et hvilket som helst af kravene 2 til 6 ved en temperatur på under 37°C.
11. Rumudfyldende gel, som understøtter cellevækst og omfatter (i) celler og (ii) polymerpartikler som defineret i et hvilket som helst af kravene 2 til 6 ved legemstemperatur.
12. Fremgangsmåde ifølge krav 1, hvor de polymeriserbare alkylenglycolacrylat-monomerer og polymeriserbare alkylenglycolmethacrylat-monomerer er valgt blandt: di(ethylenglycol)-acrylat, oligo(ethylenglycol)-acrylat, poly(ethylenglycol)-acrylat, di(ethylenglycol)-methacrylat, oligo(ethylenglycol)-methacrylat, poly(ethylenglycol)-methacrylat, poly(propylenglycol)-acrylat og poly(propylenglycol)-methacrylat.
13. Fremgangsmåde ifølge krav 1 eller krav 12, hvor den polymer, der udviser en lavere kritisk opløsningstemperatur: (a) er polymerisationsproduktet af to monomerer eller (b) er polymerisationsproduktet af poly(ethylenglycol)methacrylat (PEGMA) og po-ly(propylenglycol)methacrylat (PPGMA) eller (c) er en polymer, som har en molær masse på 25-75 kDa med et polydispersitets-(Mw/Mn)-indeks på 1-2,5, målt ved GPC.
14. Fremgangsmåde ifølge et hvilket som helst af kravene 1,12 og 13, hvor polymerpartiklerne: (a) har en bulkfase eller kerne af bionedbrydelige polymerer, som er valgt blandt poly(mælke-co-glycolsyre), poly(mælkesyre) og poly(glycolsyre); og/eller (b) i deres bulkfase eller kerne omfatter aktive bestanddele, som er valgt blandt: smagsstoffer, duftstoffer, små molekylelægemidler og biofarmaceutika og vækstfaktorer med langsom afgivelse og andre opløselige molekyler til vævsvækst, organ reparation og regenerativ medicin.
DK09748135.2T 2008-10-13 2009-10-13 Polymerpartikler, som er fremstillet af polymeriserbare alkylenglycol-(meth)acrylat-monomerer DK2346914T3 (da)

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GBGB0818747.8A GB0818747D0 (en) 2008-10-13 2008-10-13 Biocompatible responsive materials
PCT/GB2009/051362 WO2010043892A1 (en) 2008-10-13 2009-10-13 Polymer particles prepared from polymerisable alkylene glycol (meth) acrylate monomers

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US (1) US8940336B2 (da)
EP (1) EP2346914B1 (da)
JP (1) JP5718815B2 (da)
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ES (1) ES2573941T3 (da)
GB (1) GB0818747D0 (da)
WO (1) WO2010043892A1 (da)

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SG189650A1 (en) * 2011-10-17 2013-05-31 Agency Science Tech & Res Copolymers
WO2014013238A1 (en) 2012-07-16 2014-01-23 The University Of Nottingham Scaffold
US9205175B2 (en) 2012-07-27 2015-12-08 Drexel University Thermosensitive and crosslinkable polymer composite for three-dimensional soft tissue scaffold printing
SG11201700615QA (en) * 2014-08-19 2017-02-27 Univ Singapore Thermogelling graft copolymer and method of preparation thereof
JP5874859B1 (ja) * 2014-12-12 2016-03-02 東洋インキＳｃホールディングス株式会社 体液接触用医療用具および生体適合性重合体
JP6815719B2 (ja) * 2015-05-11 2021-01-20 ポーラ化成工業株式会社 海島構造を有する被膜及びこれを形成する組成物
EP3801600A4 (en) * 2018-06-05 2022-04-13 University of Mississippi Medical Center PRODUCTION OF MACRO AND MICRO ELP PARTICLES FOR ACTIVE RELEASE

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JPS62224290A (ja) * 1986-03-26 1987-10-02 Hitachi Chem Co Ltd 微生物菌体固定化法
US5173421A (en) 1988-12-14 1992-12-22 Mitsubishi Kasei Corporation Cell culture carriers
US20040195710A1 (en) 1990-10-15 2004-10-07 Hubbell Jeffrey A. Gels for encapsulation of biological materials
US6103528A (en) 1998-04-17 2000-08-15 Battelle Memorial Institute Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices
JP2001261845A (ja) * 2000-03-14 2001-09-26 Mitsubishi Chemicals Corp ハイドロゲル微粒子
FR2810669B1 (fr) * 2000-06-27 2002-08-30 Atofina Copolymeres hydrosolubles thermosensibles a base de (meth) acrylate (poly)ethoxyle, leur fabrication et leur utilisation pour la preparation de film, adhesifs et liants pour nappes fibreuses
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EP2346914B1 (en) 2016-04-27
GB0818747D0 (en) 2008-11-19
JP2012505305A (ja) 2012-03-01
WO2010043892A1 (en) 2010-04-22
US8940336B2 (en) 2015-01-27
JP5718815B2 (ja) 2015-05-13
US20110305767A1 (en) 2011-12-15
ES2573941T3 (es) 2016-06-13
EP2346914A1 (en) 2011-07-27

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