DK2344629T3 - Systemer der anvender cellekultur til fremstilling af isopren - Google Patents

Systemer der anvender cellekultur til fremstilling af isopren Download PDF

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Publication number
DK2344629T3
DK2344629T3 DK09736325.3T DK09736325T DK2344629T3 DK 2344629 T3 DK2344629 T3 DK 2344629T3 DK 09736325 T DK09736325 T DK 09736325T DK 2344629 T3 DK2344629 T3 DK 2344629T3
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Denmark
Prior art keywords
isoprene
cells
hour
polypeptide
nucleic acid
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DK09736325.3T
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English (en)
Inventor
Gopal K Chotani
Joseph C Mcauliffe
Caroline M Peres
Gregory M Whited
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Danisco Us Inc
Goodyear Tire & Rubber
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Publication of DK2344629T3 publication Critical patent/DK2344629T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (13)

1. Fremgangsmåde til fremstilling af isopren, hvilken fremgangsmåde omfatter: a) at dyrke celler omfattende en eller flere heterologe nukleinsyrer, der koder for isopren-syntase-polypeptid under egnede forhold til fremstilling af isopren, hvor en gas tilsættes ved en gasoverrislingsrate (gas sparging rate) mellem 0,01 vvm og 2 vvm; og b) at fremstille isopren, hvor cellerne producerer mere end 400 nmol/gwcm/time af isopren, og hvor kuldioxid-udviklingshastigheden af cellerne er større end 1 x 10'18 mmol/L/time.
2. Fremgangsmåden ifølge krav 1, (i) hvor fremgangsmåden yderligere omfatter at genvinde isoprenet, eller (ii) hvor cellerne yderligere omfatter en eller flere heterologe nukleinsyrer eller en eller flere yderligere kopier af en endogen nukleinsyre, der koder for et isopren-syntase-polypeptid, eller (iii) hvor cellerne yderligere omfatter en eller flere heterologe nukleinsyrer eller en eller flere yderligere kopier af en endogen nukleinsyre, der koder for et IDI-polypeptid, et MVA-omsætningsvej-enzym, eller et DXP-omsætningsvej-enzym, eller (iv) hvor isopren-syntase-polypeptidet er fra Pueraria eller Populus eller en hybrid, Populus alba x Populus tremula.
3. Fremgangsmåden ifølge krav 2(iii), hvor MVA-omsætningsvej-enzymet er mevalonat-kinase.
4. Fremgangsmåden ifølge krav 2(iv), hvor isopren-syntase-polypeptidet vælges fra gruppen bestående af Pueraria montana eller Pueraria lobata, Populus tremuloides, Populus alba, Populus nigra, og Populus trichocarpa.
5. Fremgangsmåden ifølge krav 1, hvor cellerne er grampositive bakterieceller, Streptomyces-celler, gramnegative bakterieceller, Escherichia-ceUer, Pantoea-celler, svampeceller, filamentøse svampeceller, Trichoderma-ceUer, Aspergillus-celler, eller gærceller.
6. Fremgangsmåden ifølge krav 5, hvor cellerne vælges fra gruppen bestående af Bacillus subtilis, Streptomyces lividans, Streptomyces coelicolor, Streptomyces griseus, Escherichia coli, Pantoea citrea, Trichoderma reesei, Aspergillus oryzae, Aspergillus niger, Saccharomyces cerevisiae og Yarrowia lipolytica.
7. Fremgangsmåde til fremstilling af isopren, hvilken fremgangsmåde omfatter: a) at dyrke celler omfattende en eller flere heterologe nukleinsyrer, der koder for isopren-syntase-polypeptid under egnede forhold til fremstilling af isopren, hvor en gas tilsættes ved en gasoverrislingsrate (gas sparging rate) mellem 0,01 vvm og 2 vvm; og b) at fremstille isopren, hvor isopren-koncentrationen i den flydende fase er mindre end 1 g/L og cellerne producerer mere end 400 nmol/gwcm/time af isopren.
8. Fremgangsmåden ifølge krav 7, (i) hvor fremgangsmåden yderligere omfatter at genvinde isoprenet, eller (ii) hvor cellerne yderligere omfatter en eller flere heterologe nukleinsyrer eller en eller flere yderligere kopier af en endogen nukleinsyre, der koder for et isopren-syntase-polypeptid, eller (iii) hvor cellerne yderligere omfatter en eller flere heterologe nukleinsyrer eller en eller flere yderligere kopier af en endogen nukleinsyre, der koder for et IDI-polypeptid, et MVA-omsætningsvej-enzym, eller et DXP-omsætningsvej-enzym, eller (iv) hvor isopren-syntase-polypeptidet er fra Pueraria eller Populus eller en hybrid, Populus alba x Populus tremula, eller (v) hvor cellerne er grampositive bakterieceller, Streptomyces-ceWer, gramnegative bakterieceller, Escherichia-ceUer, Pantoea-celler, svampeceller, filamentøse svampeceller, Trichoderma-ceUer, Aspergillus-celler, eller gærceller.
9. Fremgangsmåden ifølge krav 8(iii) hvor MVA-omsætningsvej-enzymet er mevalonat-kinase.
10. Fremgangsmåde til fremstilling af en forbindelse, hvor forbindelsen har et eller flere karakteristika valgt fra gruppen bestående af (a) en Henrys lovkoefficient på mindre end 250 M/atm og (b) en opløselighed i vand på mindre end 100 g/L, hvilken fremgangsmåde omfatter: a) at dyrke celler omfattende en eller flere heterologe nukleinsyrer, der koder for isopren-syntase-polypeptid under egnede forhold til fremstilling af forbindelsen, hvor en gas tilsættes ved en gasoverrislingsrate (gas sparging rate) mellem 0,01 vvm og 2 vvm; og b) at fremstille forbindelsen.
11. Fremgangsmåden ifølge krav 10, (i) hvor fremgangsmåden yderligere omfatter at genvinde forbindelsen, eller (ii) hvor den fremstillede forbindelse er ethylen, eller (iii) hvor den fremstillede forbindelse er isopren.
12. Fremgangsmåde til fremstilling af en forbindelse, hvor forbindelsen har et eller flere karakteristika valgt fra gruppen bestående af (a) en Henrys lovkoefficient på mindre end 250 M/atm og (b) en opløselighed i vand på mindre end 100 g/L, hvilken fremgangsmåde omfatter: a) at dyrke celler omfattende en eller flere heterologe nukleinsyrer, der koder for isopren-syntase-polypeptid under egnede forhold til fremstilling af forbindelsen, hvor en gas tilsættes ved en gasoverrislingsrate (gas sparging rate) mellem 0,01 vvm og 2 vvm, og hvor forbindelsen har et eller flere karakteristika valgt fra gruppen bestående af (a) en Henrys lov-koefficient på mindre end 250 M/atm og (b) en opløselighed i vand på mindre end 100 g/L; b) at fremstille forbindelsen; c) og at genvinde forbindelsen fra gasfasen.
13. Fremgangsmåden ifølge krav 12, (i) hvor den fremstillede forbindelse er ethylen, eller (ii) hvor forbindelsen er isopren.
DK09736325.3T 2008-09-15 2009-09-15 Systemer der anvender cellekultur til fremstilling af isopren DK2344629T3 (da)

Applications Claiming Priority (3)

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US9716308P 2008-09-15 2008-09-15
US18783209P 2009-06-17 2009-06-17
PCT/US2009/057039 WO2010031079A1 (en) 2008-09-15 2009-09-15 Systems using cell culture for production of isoprene

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US (2) US8569026B2 (da)
EP (2) EP3323881A1 (da)
BR (1) BRPI0918453A2 (da)
CA (1) CA2737236A1 (da)
DK (1) DK2344629T3 (da)
MY (1) MY156588A (da)
SG (1) SG169641A1 (da)
WO (1) WO2010031079A1 (da)
ZA (1) ZA201102108B (da)

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EP2344629A1 (en) 2011-07-20
EP2344629B1 (en) 2018-01-24
BRPI0918453A2 (pt) 2019-12-17
CA2737236A1 (en) 2010-03-18
US20100167371A1 (en) 2010-07-01
ZA201102108B (en) 2014-09-25
US20140073026A1 (en) 2014-03-13
SG169641A1 (en) 2011-04-29
EP3323881A1 (en) 2018-05-23
WO2010031079A1 (en) 2010-03-18
MY156588A (en) 2016-03-15
US8569026B2 (en) 2013-10-29
US9121039B2 (en) 2015-09-01

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