DK2344629T3 - Systemer der anvender cellekultur til fremstilling af isopren - Google Patents
Systemer der anvender cellekultur til fremstilling af isopren Download PDFInfo
- Publication number
- DK2344629T3 DK2344629T3 DK09736325.3T DK09736325T DK2344629T3 DK 2344629 T3 DK2344629 T3 DK 2344629T3 DK 09736325 T DK09736325 T DK 09736325T DK 2344629 T3 DK2344629 T3 DK 2344629T3
- Authority
- DK
- Denmark
- Prior art keywords
- isoprene
- cells
- hour
- polypeptide
- nucleic acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (13)
1. Fremgangsmåde til fremstilling af isopren, hvilken fremgangsmåde omfatter: a) at dyrke celler omfattende en eller flere heterologe nukleinsyrer, der koder for isopren-syntase-polypeptid under egnede forhold til fremstilling af isopren, hvor en gas tilsættes ved en gasoverrislingsrate (gas sparging rate) mellem 0,01 vvm og 2 vvm; og b) at fremstille isopren, hvor cellerne producerer mere end 400 nmol/gwcm/time af isopren, og hvor kuldioxid-udviklingshastigheden af cellerne er større end 1 x 10'18 mmol/L/time.
2. Fremgangsmåden ifølge krav 1, (i) hvor fremgangsmåden yderligere omfatter at genvinde isoprenet, eller (ii) hvor cellerne yderligere omfatter en eller flere heterologe nukleinsyrer eller en eller flere yderligere kopier af en endogen nukleinsyre, der koder for et isopren-syntase-polypeptid, eller (iii) hvor cellerne yderligere omfatter en eller flere heterologe nukleinsyrer eller en eller flere yderligere kopier af en endogen nukleinsyre, der koder for et IDI-polypeptid, et MVA-omsætningsvej-enzym, eller et DXP-omsætningsvej-enzym, eller (iv) hvor isopren-syntase-polypeptidet er fra Pueraria eller Populus eller en hybrid, Populus alba x Populus tremula.
3. Fremgangsmåden ifølge krav 2(iii), hvor MVA-omsætningsvej-enzymet er mevalonat-kinase.
4. Fremgangsmåden ifølge krav 2(iv), hvor isopren-syntase-polypeptidet vælges fra gruppen bestående af Pueraria montana eller Pueraria lobata, Populus tremuloides, Populus alba, Populus nigra, og Populus trichocarpa.
5. Fremgangsmåden ifølge krav 1, hvor cellerne er grampositive bakterieceller, Streptomyces-celler, gramnegative bakterieceller, Escherichia-ceUer, Pantoea-celler, svampeceller, filamentøse svampeceller, Trichoderma-ceUer, Aspergillus-celler, eller gærceller.
6. Fremgangsmåden ifølge krav 5, hvor cellerne vælges fra gruppen bestående af Bacillus subtilis, Streptomyces lividans, Streptomyces coelicolor, Streptomyces griseus, Escherichia coli, Pantoea citrea, Trichoderma reesei, Aspergillus oryzae, Aspergillus niger, Saccharomyces cerevisiae og Yarrowia lipolytica.
7. Fremgangsmåde til fremstilling af isopren, hvilken fremgangsmåde omfatter: a) at dyrke celler omfattende en eller flere heterologe nukleinsyrer, der koder for isopren-syntase-polypeptid under egnede forhold til fremstilling af isopren, hvor en gas tilsættes ved en gasoverrislingsrate (gas sparging rate) mellem 0,01 vvm og 2 vvm; og b) at fremstille isopren, hvor isopren-koncentrationen i den flydende fase er mindre end 1 g/L og cellerne producerer mere end 400 nmol/gwcm/time af isopren.
8. Fremgangsmåden ifølge krav 7, (i) hvor fremgangsmåden yderligere omfatter at genvinde isoprenet, eller (ii) hvor cellerne yderligere omfatter en eller flere heterologe nukleinsyrer eller en eller flere yderligere kopier af en endogen nukleinsyre, der koder for et isopren-syntase-polypeptid, eller (iii) hvor cellerne yderligere omfatter en eller flere heterologe nukleinsyrer eller en eller flere yderligere kopier af en endogen nukleinsyre, der koder for et IDI-polypeptid, et MVA-omsætningsvej-enzym, eller et DXP-omsætningsvej-enzym, eller (iv) hvor isopren-syntase-polypeptidet er fra Pueraria eller Populus eller en hybrid, Populus alba x Populus tremula, eller (v) hvor cellerne er grampositive bakterieceller, Streptomyces-ceWer, gramnegative bakterieceller, Escherichia-ceUer, Pantoea-celler, svampeceller, filamentøse svampeceller, Trichoderma-ceUer, Aspergillus-celler, eller gærceller.
9. Fremgangsmåden ifølge krav 8(iii) hvor MVA-omsætningsvej-enzymet er mevalonat-kinase.
10. Fremgangsmåde til fremstilling af en forbindelse, hvor forbindelsen har et eller flere karakteristika valgt fra gruppen bestående af (a) en Henrys lovkoefficient på mindre end 250 M/atm og (b) en opløselighed i vand på mindre end 100 g/L, hvilken fremgangsmåde omfatter: a) at dyrke celler omfattende en eller flere heterologe nukleinsyrer, der koder for isopren-syntase-polypeptid under egnede forhold til fremstilling af forbindelsen, hvor en gas tilsættes ved en gasoverrislingsrate (gas sparging rate) mellem 0,01 vvm og 2 vvm; og b) at fremstille forbindelsen.
11. Fremgangsmåden ifølge krav 10, (i) hvor fremgangsmåden yderligere omfatter at genvinde forbindelsen, eller (ii) hvor den fremstillede forbindelse er ethylen, eller (iii) hvor den fremstillede forbindelse er isopren.
12. Fremgangsmåde til fremstilling af en forbindelse, hvor forbindelsen har et eller flere karakteristika valgt fra gruppen bestående af (a) en Henrys lovkoefficient på mindre end 250 M/atm og (b) en opløselighed i vand på mindre end 100 g/L, hvilken fremgangsmåde omfatter: a) at dyrke celler omfattende en eller flere heterologe nukleinsyrer, der koder for isopren-syntase-polypeptid under egnede forhold til fremstilling af forbindelsen, hvor en gas tilsættes ved en gasoverrislingsrate (gas sparging rate) mellem 0,01 vvm og 2 vvm, og hvor forbindelsen har et eller flere karakteristika valgt fra gruppen bestående af (a) en Henrys lov-koefficient på mindre end 250 M/atm og (b) en opløselighed i vand på mindre end 100 g/L; b) at fremstille forbindelsen; c) og at genvinde forbindelsen fra gasfasen.
13. Fremgangsmåden ifølge krav 12, (i) hvor den fremstillede forbindelse er ethylen, eller (ii) hvor forbindelsen er isopren.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9716308P | 2008-09-15 | 2008-09-15 | |
US18783209P | 2009-06-17 | 2009-06-17 | |
PCT/US2009/057039 WO2010031079A1 (en) | 2008-09-15 | 2009-09-15 | Systems using cell culture for production of isoprene |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2344629T3 true DK2344629T3 (da) | 2018-03-05 |
Family
ID=41571609
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK09736325.3T DK2344629T3 (da) | 2008-09-15 | 2009-09-15 | Systemer der anvender cellekultur til fremstilling af isopren |
Country Status (9)
Country | Link |
---|---|
US (2) | US8569026B2 (da) |
EP (2) | EP3323881A1 (da) |
BR (1) | BRPI0918453A2 (da) |
CA (1) | CA2737236A1 (da) |
DK (1) | DK2344629T3 (da) |
MY (1) | MY156588A (da) |
SG (1) | SG169641A1 (da) |
WO (1) | WO2010031079A1 (da) |
ZA (1) | ZA201102108B (da) |
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2009
- 2009-09-15 DK DK09736325.3T patent/DK2344629T3/da active
- 2009-09-15 MY MYPI2011001163A patent/MY156588A/en unknown
- 2009-09-15 WO PCT/US2009/057039 patent/WO2010031079A1/en active Application Filing
- 2009-09-15 EP EP17207289.4A patent/EP3323881A1/en not_active Withdrawn
- 2009-09-15 EP EP09736325.3A patent/EP2344629B1/en not_active Not-in-force
- 2009-09-15 SG SG201101790A patent/SG169641A1/en unknown
- 2009-09-15 US US12/560,366 patent/US8569026B2/en active Active
- 2009-09-15 BR BRPI0918453A patent/BRPI0918453A2/pt not_active Application Discontinuation
- 2009-09-15 CA CA2737236A patent/CA2737236A1/en not_active Abandoned
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2011
- 2011-03-22 ZA ZA2011/02108A patent/ZA201102108B/en unknown
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2013
- 2013-09-16 US US14/028,342 patent/US9121039B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP2344629A1 (en) | 2011-07-20 |
EP2344629B1 (en) | 2018-01-24 |
BRPI0918453A2 (pt) | 2019-12-17 |
CA2737236A1 (en) | 2010-03-18 |
US20100167371A1 (en) | 2010-07-01 |
ZA201102108B (en) | 2014-09-25 |
US20140073026A1 (en) | 2014-03-13 |
SG169641A1 (en) | 2011-04-29 |
EP3323881A1 (en) | 2018-05-23 |
WO2010031079A1 (en) | 2010-03-18 |
MY156588A (en) | 2016-03-15 |
US8569026B2 (en) | 2013-10-29 |
US9121039B2 (en) | 2015-09-01 |
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