DK2313498T3 - Genkendelsessekvenser for i-crei-afledte meganukleaser og anvendelser deraf - Google Patents

Genkendelsessekvenser for i-crei-afledte meganukleaser og anvendelser deraf Download PDF

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Publication number
DK2313498T3
DK2313498T3 DK09798667.3T DK09798667T DK2313498T3 DK 2313498 T3 DK2313498 T3 DK 2313498T3 DK 09798667 T DK09798667 T DK 09798667T DK 2313498 T3 DK2313498 T3 DK 2313498T3
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DK
Denmark
Prior art keywords
dna
sequence
meganuclease
crel
recognition
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Application number
DK09798667.3T
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English (en)
Inventor
Derek Jantz
James Jefferson Smith
Original Assignee
Prec Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Publication of DK2313498T3 publication Critical patent/DK2313498T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
    • G06Q99/00—Subject matter not provided for in other groups of this subclass

Claims (5)

1. Fremgangsmåde ex vivo til spaltning af et dobbeltstrenget DNA, hvilken fremgangsmåde omfatter: (a) identificering i DNA’et af mindst ét genkendelsessted for en rationelt designet ICrel-afledt meganuklease med ændret specificitet i forhold til I-Crel, hvor genkendelsesstedet ikke spaltes af en naturligt forekommende I-Crel, hvor genkendelsesstedet identificeres baseret på tilstedeværelse af en central sekvens med fire basepar udvalgt fra gruppen bestående af TTGT, TT AT, TTTC, TTCC, TTAG, TTAC og TTGC; (b) tilvejebringelse af den rationelt designede megenuklease og (c) etablering af kontakt mellem DNA’et og den rationelt designede megenuklease; hvorved den rationelt designede megenuklease spalter DNA’et.
2. Fremgangsmåde ex vivo til spaltning af et dobbeltstrenget DNA, hvilken fremgangsmåde omfatter: (a) inkorporering af en central sekvens med fire basepar udvalgt fra gruppen bestående af TTGT, TT AT, TTTC, TTCC, TTAG, TTAC og TTGC i et genkendelsessted for en rationelt designet I-Crel-afledt meganuklease med ændret specificitet i forhold til I-Crel, hvor genkendelsesstedet ikke spaltes af en naturligt forekommende I-Crel, (b) indføring af genkendelsesstedet i DNA’et, (c) tilvejebringelse af den rationelt designede megenuklease og (d) etablering af kontakt mellem DNA’et og den rationelt designede megenuklease; hvorved den rationelt designede megenuklease spalter DNA’et.
3. Fremgangsmåde ex vivo ifølge et hvilket som helst af kravene 1 -2, hvor DNA-spaltningen sker in vitro, eventuelt hvor DNA’et er udvalgt fra gruppen bestående af et PCR-produkt; et kunstigt kromosom; genomisk DNA isoleret fra bakterier, svampe, planter eller dyreceller og viralt DNA.
4. Fremgangsmåde ex vivo ifølge et hvilket som helst af kravene 1-3, der endvidere omfatter rationelt design af den I-Crel-afledte meganuklease for at genkende genkendelsesstedet.
5. Fremgangsmåde ex vivo ifølge et hvilket som helst af kravene 1 -4, der endvidere omfatter frembringelse af den rationelt designede I-Crel-afledte meganuklease.
DK09798667.3T 2008-07-14 2009-07-14 Genkendelsessekvenser for i-crei-afledte meganukleaser og anvendelser deraf DK2313498T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8045308P 2008-07-14 2008-07-14
PCT/US2009/050566 WO2010009147A1 (en) 2008-07-14 2009-07-14 Recognition sequences for i-crei-derived meganucleases and uses thereof

Publications (1)

Publication Number Publication Date
DK2313498T3 true DK2313498T3 (da) 2017-05-22

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Family Applications (2)

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DK17160537.1T DK3211075T3 (da) 2008-07-14 2009-07-14 Genkendelsessekvenser til i-crei-afledte meganukleaser og anvendelser deraf
DK09798667.3T DK2313498T3 (da) 2008-07-14 2009-07-14 Genkendelsessekvenser for i-crei-afledte meganukleaser og anvendelser deraf

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DK17160537.1T DK3211075T3 (da) 2008-07-14 2009-07-14 Genkendelsessekvenser til i-crei-afledte meganukleaser og anvendelser deraf

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Country Link
US (7) US20110202479A1 (da)
EP (4) EP3495478A3 (da)
JP (1) JP2011527906A (da)
AU (2) AU2009271011B2 (da)
CA (1) CA2730921A1 (da)
DK (2) DK3211075T3 (da)
ES (2) ES2625941T3 (da)
WO (1) WO2010009147A1 (da)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012012444A2 (pt) 2009-11-27 2015-09-22 Basf Plant Science Co Gmbh "endonuclease quimérica, polinucleotídeo isolado, cassete de expressão, vetor, organismo não humano, método para fornecer uma endonuclease quimérica, método para recombinação homóloga de polinucleotídeos, método para mutação alvejada de polinucleotídeosa e método para recombinação homóloga ou mutação alvejada"
DE112010004583T5 (de) 2009-11-27 2012-10-18 Basf Plant Science Company Gmbh Chimäre Endonukleasen und Anwendungen davon
EP2723874A2 (en) * 2011-06-21 2014-04-30 Pioneer Hi-Bred International, Inc. Methods and compositions for producing male sterile plants
JP2017512767A (ja) 2014-03-12 2017-05-25 プレシジョン バイオサイエンシズ，インク． 改変ヌクレアーゼを用いたジストロフィン遺伝子エクソンの欠失
DK3289076T3 (da) 2015-05-01 2022-01-17 Prec Biosciences Inc Præcis sletning af kromosomale sekvenser in vivo
ES2905791T3 (es) 2016-05-03 2022-04-12 Prec Biosciences Inc Nucleasas de ingeniería útiles para el tratamiento de la hemofilia A
EP3612205B9 (en) * 2017-04-21 2023-10-04 Precision Biosciences, Inc. Engineered meganucleases specific for recognition sequences in the pcsk9 gene
WO2020157164A1 (en) 2019-01-30 2020-08-06 Enobraq Modified plant with improved rubisco activity
CN114026228A (zh) * 2019-05-07 2022-02-08 精密生物科学公司 用于识别序列的工程化大范围核酸酶的优化
US20230183664A1 (en) 2020-05-11 2023-06-15 Precision Biosciences, Inc. Self-limiting viral vectors encoding nucleases
KR20230010670A (ko) 2020-05-12 2023-01-19 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 이식유전자 발현의 drg-특이적 감소를 위한 조성물
TW202233844A (zh) 2020-10-29 2022-09-01 賓州大學委員會 Aav衣殼及含有其之組成物
WO2022232267A1 (en) 2021-04-27 2022-11-03 The Trustees Of The University Of Pennsylvania Porcine-derived adeno-associated virus capsids and uses thereof
WO2023081756A1 (en) 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
WO2023087019A2 (en) 2021-11-15 2023-05-19 The Trustees Of The University Of Pennsylvania Compositions for drg-specific reduction of transgene expression
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024015966A2 (en) 2022-07-15 2024-01-18 The Trustees Of The University Of Pennsylvania Recombinant aav having aav clade d and clade e capsids and compositions containing same
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
US5436150A (en) * 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5792640A (en) * 1992-04-03 1998-08-11 The Johns Hopkins University General method to clone hybrid restriction endonucleases using lig gene
US6395959B1 (en) * 1992-05-05 2002-05-28 Institut Pasteur Nucleotide sequence encoding the enzyme I SceI and the use thereof
US5474896A (en) * 1992-05-05 1995-12-12 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5792632A (en) * 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5830729A (en) * 1996-04-18 1998-11-03 Institut Pasteur I Sce I-induced gene replacement and gene conversion in embryonic stem cells
US6265196B1 (en) * 1996-05-07 2001-07-24 Johns Hopkins University Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
EP1147209A2 (en) * 1999-02-03 2001-10-24 The Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
WO2000046385A1 (en) * 1999-02-03 2000-08-10 The Children's Medical Center Corporation Gene repair involving in vivo excision of targeting dna
US6794136B1 (en) * 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
DE10131786A1 (de) * 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
ATE375388T1 (de) * 2001-07-27 2007-10-15 Us Gov Health & Human Serv Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden
AU2003251286B2 (en) * 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
JP2005520519A (ja) * 2002-03-15 2005-07-14 セレクティス ハイブリッドおよび単鎖メガヌクレアーゼならびにその使用
US9447434B2 (en) * 2002-09-05 2016-09-20 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
AU2003290518A1 (en) 2002-09-06 2004-04-23 Fred Hutchinson Cancer Research Center Methods and compositions concerning designed highly-specific nucleic acid binding proteins
US7285699B2 (en) * 2002-10-07 2007-10-23 Stowers Institute For Medical Research Ends-out gene targeting method
US20030175968A1 (en) * 2002-10-30 2003-09-18 Golic Kent G. Gene targeting method
EP2559759A1 (en) 2003-01-28 2013-02-20 Cellectis Custom-made meganuclease and use thereof
US7888121B2 (en) * 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
ATE514782T1 (de) 2003-11-18 2011-07-15 Bayer Bioscience Nv Verbesserte gezielte dna insertion in pflanzen
EP1591521A1 (en) 2004-04-30 2005-11-02 Cellectis I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof
WO2006097784A1 (en) 2005-03-15 2006-09-21 Cellectis I-crei meganuclease variants with modified specificity, method of preparation and uses thereof
WO2007034262A1 (en) 2005-09-19 2007-03-29 Cellectis Heterodimeric meganucleases and use thereof
ES2347684T3 (es) 2005-03-15 2010-11-03 Cellectis Variantes de la meganucleasa i-crei con especificidad modifica, metodo de preparacion y usos de las mismas.
DK2650365T3 (da) * 2005-10-18 2016-12-05 Prec Biosciences Rationelt konstruerede meganukleaser med ændret sekvensspecificitet og dna-bindingsaffinitet
WO2007060495A1 (en) 2005-10-25 2007-05-31 Cellectis I-crei homing endonuclease variants having novel cleavage specificity and use thereof
WO2007049095A1 (en) 2005-10-25 2007-05-03 Cellectis Laglidadg homing endonuclease variants having mutations in two functional subdomains and use thereof
WO2007093836A1 (en) * 2006-02-13 2007-08-23 Cellectis Meganuclease variants cleaving a dna target sequence from a xp gene and uses thereof
WO2008010009A1 (en) * 2006-07-18 2008-01-24 Cellectis Meganuclease variants cleaving a dna target sequence from a rag gene and uses thereof
WO2008059317A1 (en) 2006-11-14 2008-05-22 Cellectis Meganuclease variants cleaving a dna target sequence from the hprt gene and uses thereof
WO2008093152A1 (en) 2007-02-01 2008-08-07 Cellectis Obligate heterodimer meganucleases and uses thereof
AU2007347328B2 (en) 2007-02-19 2013-03-07 Cellectis LAGLIDADG homing endonuclease variants having novel substrate specificity and use thereof
WO2008102199A1 (en) 2007-02-20 2008-08-28 Cellectis Meganuclease variants cleaving a dna target sequence from the beta-2-microglobulin gene and uses thereof
WO2008149176A1 (en) 2007-06-06 2008-12-11 Cellectis Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof
EP2568048A1 (en) 2007-06-29 2013-03-13 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
WO2009013559A1 (en) 2007-07-23 2009-01-29 Cellectis Meganuclease variants cleaving a dna target sequence from the human hemoglobin beta gene and uses thereof
WO2009019528A1 (en) 2007-08-03 2009-02-12 Cellectis Meganuclease variants cleaving a dna target sequence from the human interleukin-2 receptor gamma chain gene and uses thereof
EP2352821B1 (en) 2008-09-08 2016-11-23 Cellectis Meganuclease variants cleaving a dna target sequence from a glutamine synthetase gene and uses thereof

Also Published As

Publication number Publication date
ES2705756T3 (es) 2019-03-26
AU2016200034B2 (en) 2018-02-15
US20190194729A1 (en) 2019-06-27
EP3211075A2 (en) 2017-08-30
AU2009271011B2 (en) 2015-10-22
CA2730921A1 (en) 2010-01-21
JP2011527906A (ja) 2011-11-10
AU2009271011A1 (en) 2010-01-21
DK3211075T3 (da) 2019-01-21
US9683257B2 (en) 2017-06-20
EP2313498B1 (en) 2017-03-15
US20170298419A1 (en) 2017-10-19
EP4001408A3 (en) 2022-06-08
EP3495478A3 (en) 2019-07-24
EP4001408A2 (en) 2022-05-25
US10287626B2 (en) 2019-05-14
EP3495478A2 (en) 2019-06-12
US20210130874A1 (en) 2021-05-06
US10273524B2 (en) 2019-04-30
EP3211075B1 (en) 2018-10-24
EP3211075A3 (en) 2017-10-04
US20230088311A1 (en) 2023-03-23
ES2625941T3 (es) 2017-07-21
US20150050655A1 (en) 2015-02-19
EP2313498A1 (en) 2011-04-27
US20110202479A1 (en) 2011-08-18
US20170298420A1 (en) 2017-10-19
EP2313498A4 (en) 2011-10-05
AU2016200034A1 (en) 2016-01-28
WO2010009147A1 (en) 2010-01-21

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