DK2310413T3 - Kamelidafledte antigenbindende polypeptider - Google Patents
Kamelidafledte antigenbindende polypeptider Download PDFInfo
- Publication number
- DK2310413T3 DK2310413T3 DK09772912.3T DK09772912T DK2310413T3 DK 2310413 T3 DK2310413 T3 DK 2310413T3 DK 09772912 T DK09772912 T DK 09772912T DK 2310413 T3 DK2310413 T3 DK 2310413T3
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- human
- antigen
- binding polypeptide
- antibodies
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Claims (12)
1. Monoklonalt antigenbindende polypeptid omfattende et VH-domæne og et VL-domæne, hvori hver af de hypervariable sløjfer i både VH-domænet og VL-domænet opnås fra et VH- eller VL-domæne af en art i familien Camelidae, hvori hypervariabel sløjfe H1 og hypervariabel sløjfe H2 i VH-domænet hver udviser en forudsagt eller faktisk kanonisk foldestruktur, som er identisk med en kanonisk foldestruktur, som forekommer i humane antistoffer, og hvori hypervariabel sløjfe H1 og hypervariabel sløjfe H2 i VH-domænet danner en kombination af forudsagte eller faktiske kanoniske foldestrukturer, som er identiske med en kombination af kanoniske foldestrukturer som er kendt at forekomme i et humant kimlinje-VH-domæne.
2. Antigenbindende polypeptid ifølge krav 1 omfattende et VH-domæne, hvori VH-domænet udviser en sekvensidentitet på 80% eller mere, 85% eller mere, 90% eller mere, 95% eller mere, eller 97% eller mere med et eller flere humane VH-domæner på tværs af strukturområderne FR1, FR2, FR3 og FR4, og hvori VL-domænet udviser en sekvensidentitet på 80% eller mere, 85% eller mere, 90% eller mere, 95% eller mere, eller 97% eller mere med et eller flere humane VL-domæner på tværs af strukturområderne FR1, FR2, FR3 og FR4.
3. Antigenbindende polypeptid ifølge krav 1, som udviser en sekvensidentitet på 80% eller mere med et humant VH-domæne på tværs af strukturområderne FR1, FR2, FR3 og FR4, og hvori hypervariabel sløjfe H1 og hypervariabel sløjfe H2 danner en kombination af forudsagte eller faktiske kanoniske foldestrukturer, som er det samme som en kanonisk foldekombination, som er kendt at forekomme naturligt i samme humane VH-domænet.
4. Antigenbindende polypeptid ifølge ethvert af kravene 1 til 3, hvori hypervariabel sløjfe L1 og hypervariabel sløjfe L2 i VL-domænet hver er opnået fra et VH- eller VL-domæne af en art i familien Camelidae og hver udviser et forudsagt eller faktisk kanonisk foldestruktur, som er identisk med en kanonisk foldestruktur, som forekommer i humane antistoffer, og hvori hypervariabel sløjfe L1 og hypervariabel sløjfe L2 i VL-domænet danner en kombination af forudsagte eller faktiske kanoniske foldestrukturer, som er identiske med en kombination af kanoniske foldestrukturer, som er kendt at forekomme i et humant kimlinje-VL-domæne.
5. Antigenbindende polypeptid ifølge et hvilket som helst af de foregående krav, hvori arten i familien Camelidae er valgt fra gruppen bestående af kamel, lama, dromedar, vikunja, guanako og alpaka.
6. Antigenbindende polypeptid ifølge et hvilket som helst af de foregående krav, som er immunoreaktiv med eller specifikt binder til et target-antigen, nævnte target-antigen er et ikke-kamelidantigen, eller et humant antigen eller et viralt antigen eller et bakterielt antigen, eller en target af terapeutisk eller diagnostisk betydning.
7. Antigenbindende polypeptid ifølge et hvilket som helst af de foregående krav, som er et kimærisk polypeptid, der omfatter mindst et konstant domæne med en aminosyresekvens, der kodes af et humant immunglobulingen, eller en aminosyresekvens, som er mindst 90% identisk dermed, eller som omfatter den komplette konstante region af et humant antistof.
8. Antigenbindende polypeptid ifølge et hvilket som helst af de foregående krav, som er et antistof, Fab, Fab', F(ab')2, bispecifikke Fab', Fv-fragmenter, diabody, lineært antistof, et enkeltkædet variabelt fragment (scFv) eller multispecifikt antistof dannet fra antistoffragmenter.
9. Polynukleotidmolekyle, der koder for et antigenbindende polypeptid ifølge et hvilket som helst af kravene 1 til 8.
10. Ekspressionsvektor omfattende polynukleotidmolekylet ifølge krav 9 operativt koblet til regulatoriske sekvenser, som tillader ekspression af det antigenbindende polypeptid i en værtscelle eller et cellefrit ekspressionssystem.
11. Værtscelle eller cellefrit ekspressionssystem indeholdende ekspressionsvektoren ifølge krav 10.
12. Fremgangsmåde til at fremstille et rekombinant antigenbindende polypeptid, som omfatter dyrkning af værtscellen eller det cellefrie ekspressionssystem ifølge krav 11 under betingelser, som tillader ekspression af det antigenbindende polypeptid og udvinding af det udtrykte antigenbindende polypeptid.
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| GB0812120A GB2461546B (en) | 2008-07-02 | 2008-07-02 | Antigen binding polypeptides |
| US11016108P | 2008-10-31 | 2008-10-31 | |
| PCT/IB2009/006329 WO2010001251A2 (en) | 2008-07-02 | 2009-07-02 | Antigen binding polypeptides |
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| WO (1) | WO2010001251A2 (da) |
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| GB0812120D0 (en) | 2008-08-06 |
| AU2009265278B2 (en) | 2015-03-19 |
| CA2728076A1 (en) | 2010-01-07 |
| WO2010001251A2 (en) | 2010-01-07 |
| JP2011526493A (ja) | 2011-10-13 |
| CA2728076C (en) | 2020-05-19 |
| WO2010001251A3 (en) | 2010-03-11 |
| CN102216328A (zh) | 2011-10-12 |
| GB2461546B (en) | 2010-07-07 |
| EP3061768A1 (en) | 2016-08-31 |
| EP2310413B1 (en) | 2017-03-01 |
| GB2461546A (en) | 2010-01-06 |
| CN107739405A (zh) | 2018-02-27 |
| AU2009265278A1 (en) | 2010-01-07 |
| EP2310413A2 (en) | 2011-04-20 |
| JP6216940B2 (ja) | 2017-10-25 |
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