DK2279253T3 - Konstruktion og tilførsel af terapeutiske sammensætninger af frisk isolerede celler - Google Patents
Konstruktion og tilførsel af terapeutiske sammensætninger af frisk isolerede celler Download PDFInfo
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Claims (4)
1. Ex-vivo-fremgangsmåde til transient at modificere ustimulerede hvilende perifere blodmononukleare celler (PBMC'er) til at eksprimere en kimerisk receptor på deres overflade, hvilken fremgangsmåde omfatter følgende trin: (a) isolering af ustimulerede hvilende PBMC'erne fra perifert blod, som tidligere er opsamlet; og (b) elektroladning af PBMC'erne med mRNA, som koder for en kimerisk receptor, hvor de elektroladede PBMC'er transient eksprimerer den kimeriske receptor på deres overflade, og hvor elektroladningen er strømnings-elektroporation.
2. Fremgangsmåde ifølge krav 1, hvor PBMC'erne er perifere blodlymfocyter (PBL'er), naturlige killer-(NK)-celler, eller T-celler.
3. Fremgangsmåde ifølge krav 2, hvor trinnet med isolering af NK-cellerne omfatter (i) separation af CD56+-celler fra blod eller trinnet med (ii) depletering af CD3+-celler fra blod.
4. Fremgangsmåde ifølge krav 1, hvor den kimeriske receptor er en anti-CD19 kimerisk receptor.
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| PCT/US2009/040040 WO2009126789A2 (en) | 2008-04-09 | 2009-04-09 | Engineering and delivery of therapeutic compositions of freshly isolated cells |
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| DK2279253T3 true DK2279253T3 (da) | 2017-02-13 |
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| US9249423B2 (en) | 2007-02-02 | 2016-02-02 | Yale University | Method of de-differentiating and re-differentiating somatic cells using RNA |
| US10155038B2 (en) | 2007-02-02 | 2018-12-18 | Yale University | Cells prepared by transient transfection and methods of use thereof |
| US8859229B2 (en) * | 2007-02-02 | 2014-10-14 | Yale University | Transient transfection with RNA |
| DK2279253T3 (da) * | 2008-04-09 | 2017-02-13 | Maxcyte Inc | Konstruktion og tilførsel af terapeutiske sammensætninger af frisk isolerede celler |
| KR102243575B1 (ko) * | 2010-12-09 | 2021-04-22 | 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 | 암을 치료하기 위한 키메릭 항원 수용체 변형 t 세포의 용도 |
| JP5989016B2 (ja) * | 2011-06-24 | 2016-09-07 | 国立大学法人九州大学 | Ｎｋ細胞の増幅方法 |
| JP5572863B2 (ja) * | 2011-06-24 | 2014-08-20 | 国立大学法人九州大学 | Ｎｋ細胞の増幅方法 |
| AU2012308205A1 (en) | 2011-09-16 | 2014-03-13 | The Trustees Of The University Of Pennsylvania | RNA engineered T cells for the treatment of cancer |
| US9272002B2 (en) | 2011-10-28 | 2016-03-01 | The Trustees Of The University Of Pennsylvania | Fully human, anti-mesothelin specific chimeric immune receptor for redirected mesothelin-expressing cell targeting |
| CA3209571A1 (en) | 2012-03-23 | 2013-09-26 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Anti-mesothelin chimeric antigen receptors |
| US10144770B2 (en) | 2013-10-17 | 2018-12-04 | National University Of Singapore | Chimeric receptors and uses thereof in immune therapy |
| SG10201808825XA (en) | 2014-04-10 | 2018-11-29 | Seattle Childrens Hospital Dba Seattle Childrens Res Inst | Defined composition gene modified t-cell products |
| WO2016095934A2 (en) * | 2014-12-14 | 2016-06-23 | El Abd Hisham Mohamed Magdy | A novel genetic device to engineer cell behavior |
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| WO2017004252A1 (en) | 2015-06-30 | 2017-01-05 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Redirected cells with mhc chimeric receptors and methods of use in immunotherapy |
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