DK2184350T3 - Cellulaser med forbedret termostabilitet - Google Patents

Cellulaser med forbedret termostabilitet Download PDF

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Publication number
DK2184350T3
DK2184350T3 DK09014733.1T DK09014733T DK2184350T3 DK 2184350 T3 DK2184350 T3 DK 2184350T3 DK 09014733 T DK09014733 T DK 09014733T DK 2184350 T3 DK2184350 T3 DK 2184350T3
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Denmark
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gly
ala
ser
leu
thr
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DK09014733.1T
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English (en)
Inventor
Anthony G Day
Colin Mitchinson
Andrew Shaw
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Danisco Us Inc
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Publication of DK2184350T3 publication Critical patent/DK2184350T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (7)

  1. CELLULASER MED FORBEDRET TERMOSTABILITET
    1. Forbedret cellulose omfattende en aminosyresekvens, der er blevet modificeret fra en precursoraminosyresekvens, der har sekvensen T. reesei EGIII-cellulase vist i figur 11 ved substitution af én eller flere aminosyrerester, hvilken substitution er udvalgt fra: T11S/T161, G41A, N91D, S143T, T163S, N167S, V210C og T145E/Y147W, hvilken aminosyrenummerering er som i figur 11; hvor den forbedrede cellulase har øget termostabilitet sammenlignet med en cellulase svarende til precursoraminosyresekvensen.
  2. 2. Forbedret cellulose, der omfatter en aminosyresekvens, der er blevet modificeret fra en precursoraminosyresekvens, der har sekvensen H. grisea EGIII-cellulase vist i figur 11 ved substitution af én eller flere aminosyrerester, hvilken substitution er udvalgt fra: C210V og C170G, hvilken aminosyrenummerering er som i figur 11; hvor den forbedrede cellulase har øget termostabilitet sammenlignet med en cellulase svarende til precursoraminosyresekvensen.
  3. 3. DNA-sekvens, der koder for en cellulase ifølge krav 1 eller 2.
  4. 4. Ekspressionsvektor, der omfatter en DNA-sekvens ifølge krav 3.
  5. 5. Værtscelle, der omfatter en DNA-sekvens ifølge krav 3 eller en ekspressionsvektor ifølge krav 4.
  6. 6. Fremgangsmåde til fremstilling af en cellulase ifølge krav 1 eller 2, der omfatter: (a) modificering af en DNA-sekvens, der koder for precursoraminosyresekvensen til fremstilling af en modificeret DNA-sekvens, der består af sekvensen som defineret ifølge krav 3; (b) ekspression af den modificerede DNA-sekvens i en værtscelle og (c) oprensning af det udtrykte cellulaseprotein.
  7. 7. Anvendelse af en cellulase ifølge krav 1 eller 2 i biomassereduktion, rengøringsprodukter eller tekstilbehandlingssammensætninger.
DK09014733.1T 1999-12-23 2000-12-14 Cellulaser med forbedret termostabilitet DK2184350T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47083299A 1999-12-23 1999-12-23
EP00984363A EP1240524B1 (en) 1999-12-23 2000-12-14 Cellulase from t. reesei with improved thermostability

Publications (1)

Publication Number Publication Date
DK2184350T3 true DK2184350T3 (da) 2014-11-24

Family

ID=23869235

Family Applications (2)

Application Number Title Priority Date Filing Date
DK00984363.2T DK1240524T3 (da) 1999-12-23 2000-12-14 Cellulase fra T. Reesei med forbedret termostabilitet
DK09014733.1T DK2184350T3 (da) 1999-12-23 2000-12-14 Cellulaser med forbedret termostabilitet

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK00984363.2T DK1240524T3 (da) 1999-12-23 2000-12-14 Cellulase fra T. Reesei med forbedret termostabilitet

Country Status (10)

Country Link
EP (2) EP1240524B1 (da)
JP (1) JP4658433B2 (da)
AT (1) ATE488770T1 (da)
AU (1) AU2099301A (da)
CA (1) CA2394740C (da)
DE (1) DE60045255D1 (da)
DK (2) DK1240524T3 (da)
ES (1) ES2354866T3 (da)
PT (1) PT1240524E (da)
WO (1) WO2001047956A2 (da)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6407046B1 (en) 1998-09-03 2002-06-18 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6579841B1 (en) * 1998-12-18 2003-06-17 Genencor International, Inc. Variant EGIII-like cellulase compositions
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
DE60143086D1 (de) * 2000-08-04 2010-10-28 Genencor Int Mutierte trichoderma reesei egiii cellulasen, dafür kodierende dna und verfahren zu deren herstellung
US6635465B1 (en) * 2000-08-04 2003-10-21 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6623949B1 (en) 2000-08-04 2003-09-23 Genencor International, Inc. Variant EGIII-like cellulase compositions
JP2003250544A (ja) * 2002-03-04 2003-09-09 National Institute Of Technology & Evaluation タンパク質の性質を改変する方法
SI1627050T1 (sl) * 2003-04-01 2014-01-31 Danisco Us Inc. Varianta humicola grisea cbh1.1
BRPI0509671A (pt) * 2004-04-08 2007-10-30 Genencor Internation Inc alfa-amilases mutante
US7629158B2 (en) 2006-06-16 2009-12-08 The Procter & Gamble Company Cleaning and/or treatment compositions
RU2009149405A (ru) * 2007-05-30 2011-07-10 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН (US) Улучшенные варианты альфа-амилазы bacillus licheniformis
FI122028B (fi) * 2008-12-30 2011-07-29 Ab Enzymes Oy Sieniperäiset endoglukanaasit, niiden tuotto ja käyttö
CA2771071C (en) 2009-08-07 2020-03-10 Danisco Us Inc. Alpha-amylase blend for starch processing and method of use thereof
WO2018150260A1 (en) * 2017-02-16 2018-08-23 Institut Pasteur Method for the identification of mutated proteins having modified thermal stability
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
KR100322793B1 (ko) * 1993-02-11 2002-06-20 마가렛 에이.혼 산화안정성알파-아밀라아제
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
DE69534513T2 (de) * 1994-03-08 2006-07-27 Novozymes A/S Neuartige alkalische zellulasen
DE69637940D1 (de) * 1995-02-03 2009-07-09 Novozymes As Eine methode zum entwurf von alpha-amylase mutanten mit vorbestimmten eigenschaften
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
US6211134B1 (en) * 1996-05-14 2001-04-03 Genecor International, Inc. Mutant α-amylase
ATE293696T1 (de) * 1996-12-09 2005-05-15 Genencor Int Mutierte alpha-amylase enzyme mit erhöhter stabilität
DK1038008T3 (da) * 1997-12-16 2007-03-12 Genencor Int Fremgangsmåde til fremstilling af EGIII-lignende enzymer
US6187732B1 (en) * 1998-09-03 2001-02-13 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6623949B1 (en) * 2000-08-04 2003-09-23 Genencor International, Inc. Variant EGIII-like cellulase compositions

Also Published As

Publication number Publication date
EP2184350B1 (en) 2014-09-03
JP4658433B2 (ja) 2011-03-23
EP1240524A2 (en) 2002-09-18
ES2354866T3 (es) 2011-03-18
AU2099301A (en) 2001-07-09
CA2394740C (en) 2011-02-01
EP2184350A3 (en) 2010-06-02
PT1240524E (pt) 2011-02-02
JP2004500815A (ja) 2004-01-15
WO2001047956A2 (en) 2001-07-05
EP2184350A2 (en) 2010-05-12
DK1240524T3 (da) 2011-02-14
ATE488770T1 (de) 2010-12-15
DE60045255D1 (de) 2010-12-30
CA2394740A1 (en) 2001-07-05
WO2001047956A3 (en) 2002-02-14
EP1240524B1 (en) 2010-11-17

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