DK2097525T3 - Fremgangsmåder til reduktion eller eliminering af ekspression af gener i filamentøse svampestammer ved hjælp af transitiv rna-interferens - Google Patents

Fremgangsmåder til reduktion eller eliminering af ekspression af gener i filamentøse svampestammer ved hjælp af transitiv rna-interferens

Info

Publication number
DK2097525T3
DK2097525T3 DK07865933.1T DK07865933T DK2097525T3 DK 2097525 T3 DK2097525 T3 DK 2097525T3 DK 07865933 T DK07865933 T DK 07865933T DK 2097525 T3 DK2097525 T3 DK 2097525T3
Authority
DK
Denmark
Prior art keywords
genes
reducing
methods
rna interference
filamentous fungal
Prior art date
Application number
DK07865933.1T
Other languages
English (en)
Inventor
Howard Brody
Donna Moyer
Amanda Fisher
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DK2097525T3 publication Critical patent/DK2097525T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK07865933.1T 2006-12-21 2007-12-20 Fremgangsmåder til reduktion eller eliminering af ekspression af gener i filamentøse svampestammer ved hjælp af transitiv rna-interferens DK2097525T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87689506P 2006-12-21 2006-12-21
PCT/US2007/088447 WO2008080017A1 (en) 2006-12-21 2007-12-20 Methods of reducing or eliminating expression of genes in filamentous fungal strains by transitive rna interference

Publications (1)

Publication Number Publication Date
DK2097525T3 true DK2097525T3 (da) 2013-09-23

Family

ID=39402583

Family Applications (1)

Application Number Title Priority Date Filing Date
DK07865933.1T DK2097525T3 (da) 2006-12-21 2007-12-20 Fremgangsmåder til reduktion eller eliminering af ekspression af gener i filamentøse svampestammer ved hjælp af transitiv rna-interferens

Country Status (6)

Country Link
US (2) US9499820B2 (da)
EP (1) EP2097525B1 (da)
JP (1) JP5951924B2 (da)
CN (2) CN101652479A (da)
DK (1) DK2097525T3 (da)
WO (1) WO2008080017A1 (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010129287A2 (en) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
MX350399B (es) 2009-12-18 2017-09-06 Novozymes Inc Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa.
AU2012298713B2 (en) 2011-08-24 2017-11-23 Novozymes, Inc. Methods for obtaining positive transformants of a filamentous fungal host cell
CA2861697C (en) 2012-01-05 2022-03-15 Novartis Ag Protease deficient filamentous fungal cells and methods of use thereof
BR112014028764A2 (pt) 2012-05-18 2017-06-27 Novozymes As mutante isolado, métodos para a obtenção do mutante isolado e de um lactobacillus transformante, e, lactobacillus transformante.
CN104603269A (zh) * 2012-08-17 2015-05-06 诺维信公司 用于在丝状真菌菌株中共沉默基因表达的方法及其应用
WO2014068010A1 (en) 2012-10-31 2014-05-08 Novozymes A/S Isopropanol production by bacterial hosts
WO2014076232A2 (en) 2012-11-19 2014-05-22 Novozymes A/S Isopropanol production by recombinant hosts using an hmg-coa intermediate
US20190216697A1 (en) * 2018-01-18 2019-07-18 Nohbo,LLC Hygiene product pod and methods of using same
EP3894565A1 (en) 2018-12-12 2021-10-20 Novozymes A/S Methods for increasing the productivity of a filamentous fungal cell in the production of a polypeptide
BR112022014573A2 (pt) 2020-01-24 2022-10-04 Novozymes As Mutantes de uma célula fúngica filamentosa com produtividade aumentada na produção de um polipeptídeo
WO2023003683A2 (en) 2021-07-19 2023-01-26 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells
CN118574921A (zh) 2021-12-01 2024-08-30 丹尼斯科美国公司 用于增强真菌细胞中的蛋白质产生的组合物和方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
WO2001049844A1 (en) 1999-12-30 2001-07-12 Rutgers, The State University Of New Jersey Compositions and methods for gene silencing
US20050214263A1 (en) 2001-12-12 2005-09-29 Vaistij Fabian E Methods and means for gene silencing in plants
US7422853B1 (en) * 2002-10-04 2008-09-09 Myriad Genetics, Inc. RNA interference using a universal target
EP3502252B1 (en) * 2003-06-02 2023-04-05 University of Massachusetts Methods and compositions for controlling efficacy of rna silencing
US20080044906A1 (en) 2003-09-12 2008-02-21 Peter Michael Waterhouse Modified Gene-Silencing Nucleic Acid Molecules and Uses Thereof
US8716023B2 (en) * 2003-12-09 2014-05-06 Novozymes, Inc. Methods for eliminating or reducing the expression of a genes in a filamentous fungal strains
US7323310B2 (en) * 2004-08-31 2008-01-29 Qiagen North American Holdings, Inc. Methods and compositions for RNA amplification and detection using an RNA-dependent RNA-polymerase
US20100169996A1 (en) * 2007-01-19 2010-07-01 Lionel Navarro Methods and compositions for modulating the sirna and rna-directed-dna methylation pathways

Also Published As

Publication number Publication date
US20100055755A1 (en) 2010-03-04
WO2008080017A1 (en) 2008-07-03
JP2010514419A (ja) 2010-05-06
US9499820B2 (en) 2016-11-22
CN101652479A (zh) 2010-02-17
JP5951924B2 (ja) 2016-07-13
EP2097525B1 (en) 2013-06-19
CN105420268A (zh) 2016-03-23
US20170029821A1 (en) 2017-02-02
EP2097525A1 (en) 2009-09-09

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