BRPI0911966A2 - Expressão heteróloga de celobioidrolases de fungo em levedura - Google Patents

Expressão heteróloga de celobioidrolases de fungo em levedura

Info

Publication number
BRPI0911966A2
BRPI0911966A2 BRPI0911966-3A BRPI0911966A BRPI0911966A2 BR PI0911966 A2 BRPI0911966 A2 BR PI0911966A2 BR PI0911966 A BRPI0911966 A BR PI0911966A BR PI0911966 A2 BRPI0911966 A2 BR PI0911966A2
Authority
BR
Brazil
Prior art keywords
yeast
heterologous expression
fungal cellobiohydrolases
cellobiohydrolases
fungal
Prior art date
Application number
BRPI0911966-3A
Other languages
English (en)
Inventor
Riaan Den Hann
Emile Van Zyl
Danie Lagrange
Original Assignee
Universiteit Stellenbosch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiteit Stellenbosch filed Critical Universiteit Stellenbosch
Publication of BRPI0911966A2 publication Critical patent/BRPI0911966A2/pt
Publication of BRPI0911966B1 publication Critical patent/BRPI0911966B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0911966A 2008-05-11 2009-05-11 polinucleotídeo codificando uma celobioidrolase BRPI0911966B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5221308P 2008-05-11 2008-05-11
PCT/IB2009/005881 WO2009138877A2 (en) 2008-05-11 2009-05-11 Heterologous expression of fungal cellobiohydrolases in yeast

Publications (2)

Publication Number Publication Date
BRPI0911966A2 true BRPI0911966A2 (pt) 2015-07-28
BRPI0911966B1 BRPI0911966B1 (pt) 2018-11-13

Family

ID=41151793

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0911966A BRPI0911966B1 (pt) 2008-05-11 2009-05-11 polinucleotídeo codificando uma celobioidrolase

Country Status (4)

Country Link
US (2) US9365842B2 (pt)
BR (1) BRPI0911966B1 (pt)
CA (2) CA2724076C (pt)
WO (1) WO2009138877A2 (pt)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009138877A2 (en) 2008-05-11 2009-11-19 Universiteit Stellenbosch Heterologous expression of fungal cellobiohydrolases in yeast
WO2009146464A2 (en) * 2008-05-30 2009-12-03 Edenspace Systems Corporation Methods and compositions for modifying levels of lignin and increasing the digestibility of cellulose by the recombinant overexpression of lignocellulolytic enzymes
AU2009316309B2 (en) 2008-11-21 2016-07-07 Lallemand Hungary Liquidity Management Llc Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose
BR122018013596B1 (pt) 2009-10-26 2024-01-30 Universiteit Stellenbosch (Also Known As Stellenbosch University) Células hospedeiras de saccharomyces cerevisiae bem como processos para hidrólise de um substrato celulósico e para fermentação de celulose
ES2549328T3 (es) * 2010-02-11 2015-10-27 Süd-Chemie Ip Gmbh & Co. Kg Enzimas celulasas optimizadas
AU2011214327B2 (en) * 2010-02-11 2014-09-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
BR112012028290B1 (pt) 2010-05-05 2021-02-02 Lallemand Hungary Liquidity Management Llc. levedura recombinante, processo para converter biomassa em etanol e meio de fermentação compreendendo dita levedura
CN105441414A (zh) 2010-06-01 2016-03-30 加州理工学院 稳定的功能嵌合纤维二糖水解酶i类酶
JP2014502144A (ja) * 2010-10-06 2014-01-30 ビーピー・コーポレーション・ノース・アメリカ・インコーポレーテッド バリアントｃｂｈｉポリペプチド
BR112013019039A2 (pt) 2011-01-26 2020-07-21 Novozymes A/S Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula originária, para produzir uma proteína, para degradar um material celulósico, para produzir um produto de fermentação, 5 e parafermentar um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado
EP3235903B1 (en) 2011-01-26 2021-07-07 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
BR112013018695B1 (pt) 2011-01-26 2021-03-30 Novozymes A / S Célula hospedeira microbiana recombinante, processos para produzir um polipeptídeo, para produzir uma proteína, para degradar um material celulósico, e, construto de ácido nucleico ou vetor de expressão
WO2012103350A1 (en) * 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
BR122019017739B1 (pt) 2011-04-05 2021-06-22 Lallemand Hungary Liquidity Management Llc Micro-organismo recombinante compreendendo uma deleção de enzimas nativas que atuam para produzir glicerol e/ou regular a síntese de glicerol e vias metabólicas sintéticas para converter uma fonte de carboidrato a etanol
CA2878019A1 (en) 2011-12-20 2013-06-27 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
BR112014031526A2 (pt) * 2012-06-19 2017-08-01 Dsm Ip Assets Bv promotores para expressar um gene em uma célula
EP2931887A1 (en) 2012-12-12 2015-10-21 Danisco US Inc. Variants of cellobiohydrolases
EP2970861A2 (en) 2013-03-15 2016-01-20 Lallemand Hungary Liquidity Management LLC Expression of beta-glucosidases for hydrolysis of lignocellulose and associated oligomers
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
MX2016001315A (es) 2013-07-29 2016-04-07 Danisco Us Inc Variantes de enzimas.
US11753656B2 (en) 2013-08-15 2023-09-12 Lallemand Hungary Liquidity Management Llc Methods for the improvement of product yield and production in a microorganism through glycerol recycling
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
BR122022015725B1 (pt) 2017-05-23 2023-03-14 Lallemand Hungary Liquidity Management Llc Processo para a produção de um produto de fermentação
WO2023028521A1 (en) * 2021-08-24 2023-03-02 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced cellobiohydrolase i production in s. cerevisiae
GB202115470D0 (en) * 2021-10-27 2021-12-08 Univ Stellenbosch Recombinant saccharomyces cerevisiae strains for enzymatic hydrolysis of bioplastic polymers

Family Cites Families (10)

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Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
ES2076544T3 (es) 1990-08-28 1995-11-01 Du Pont Procedimiento para seleccion rapida de vectores de secrecion eficaces.
WO1993024631A1 (en) 1992-05-29 1993-12-09 E.I. Du Pont De Nemours And Company PRODUCTION OF STREPTAVIDIN FROM $i(BACILLUS SUBTILIS)
DK2298868T3 (en) * 2001-06-26 2015-04-20 Novozymes As Polypeptides with cellobiohydrolase I activity and the same coding polynucleotides
PL1627050T3 (pl) 2003-04-01 2013-10-31 Danisco Us Inc Wariant CBH1.1 Humicola grisea
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
AU2006338208B2 (en) 2006-02-10 2013-10-24 Bp Corporation North America Inc. Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
CA2670102A1 (en) 2006-11-22 2008-05-29 The Trustees Of Dartmouth College Recombinant yeast strains expressing tethered cellulase enzymes
WO2009138877A2 (en) 2008-05-11 2009-11-19 Universiteit Stellenbosch Heterologous expression of fungal cellobiohydrolases in yeast
WO2010005553A1 (en) * 2008-07-07 2010-01-14 Mascoma Corporation Isolation and characterization of schizochytrium aggregatum cellobiohydrolase i (cbh 1)

Also Published As

Publication number Publication date
CA2724076C (en) 2018-11-27
WO2009138877A3 (en) 2010-01-07
US11530428B2 (en) 2022-12-20
US20110124074A1 (en) 2011-05-26
CA2724076A1 (en) 2009-11-19
US20160326560A1 (en) 2016-11-10
CA3021166C (en) 2024-01-09
US9365842B2 (en) 2016-06-14
CA3021166A1 (en) 2009-11-19
BRPI0911966B1 (pt) 2018-11-13
WO2009138877A2 (en) 2009-11-19

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 11/05/2009, OBSERVADAS AS CONDICOES LEGAIS.