DK2083634T3 - Native kartoffelproteinisolater - Google Patents

Native kartoffelproteinisolater Download PDF

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Publication number
DK2083634T3
DK2083634T3 DK07870595.1T DK07870595T DK2083634T3 DK 2083634 T3 DK2083634 T3 DK 2083634T3 DK 07870595 T DK07870595 T DK 07870595T DK 2083634 T3 DK2083634 T3 DK 2083634T3
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DK
Denmark
Prior art keywords
isolate
protease inhibitor
patatin
protein
potato protein
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Application number
DK07870595.1T
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English (en)
Inventor
Marco Luigi Federico Giuseppin
Marc Christiaan Laus
Der Sluis Catrinus Van
Original Assignee
Coöperatie Avebe U A
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Application filed by Coöperatie Avebe U A filed Critical Coöperatie Avebe U A
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Publication of DK2083634T3 publication Critical patent/DK2083634T3/da

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/006Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/16Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste water of starch-manufacturing plant or like wastes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/66Proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/185Vegetable proteins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Dispersion Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Medicinal Preparation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Claims (10)

1. Fremgangsmåde til opnåelse af et nativt kartoffelproteinisolat, hvilken fremgangsmåde omfatter - udsættelse af kartoffelsaft for en flokkulation med en divalent metalkation ved et pH på 7-9; - centrifugering af den flokkulerede kartoffelsaft, hvorved der dannes en ovenstående væske; - udsættelse af den ovenstående væske for expanded bed adsorptionskromatografi, der foretages ved et pH på mindre end 11 og en temperatur på 5-35 °C ved anvendelse af et mixed-mode adsorptionsmiddel, hvilket adsorptionsmiddel kan binde kartoffelprotein, hvorved det native kartoffelprotein adsorberes til adsorptionsmidlet; og - eluering af det native kartoffelproteinisolat fra absorptionsmidlet med et elueringsmiddel; hvor det native kartoffelproteinisolat elueres ved et pH på 5,7 - 8,7, hvis det native kartoffelproteinisolat er et nativt kartoffelproteinpatatinisolat, hvor det native kartoffelproteinisolat elueres ved et pH på 5,8 -12,0, hvis det native kartoffelproteinisolat er et nativt kartoffelproteaseinhibitorisolat, og hvor det native kartoffelproteinisolat elueres ved et pH på 4-12, hvis det native kartoffelproteinisolat er et totalt nativt kartoffelproteinisolat.
2. Fremgangsmåde ifølge krav 1, hvor det totale native kartoffelproteinisolat har et isoelektrisk punkt over 4,8, en molekylvægt på over 4 kDa og en glycoalkaloidkoncentration på mindre end 150 ppm.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor det native kartoffelproteinproteaseinhibitorisolat endvidere er forarbejdet til mindst ét isolat, der er udvalgt fra gruppen bestående af et proteaseinhibitor I-isolat, et carboxypeptidaseinhibitorisolat, et proteaseinhibitor Ila- og Ilb-isolat og et proteaseinhibitor A5-isolat ved ionbytning eller gelpermeationskromatografi.
4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvilken fremgangsmåde efterfølges af en ultrafiltrering, fortrinsvis ved et pH på under 6,2, for at fjerne glycoalkaloider.
5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvilken fremgangsmåde efterfølges af et trin med spraytørring eller opkoncentrering ved frysning.
6. Nativt kartoffelproteinpatatinisolat, der kan opnås ved hjælp af en fremgangsmåde ifølge krav 1 med et isoelektrisk punkt under 5,8, fortrinsvis på 4,8-5,5, en molekylvægt på over 30 kDa, fortrinsvis over 35 kDa, og en glycoalkaloidkoncentration på mindre end 150 ppm.
7. Nativt kartoffelproteinproteaseinhibitorisolat, der kan opnås ved hjælp af en fremgangsmåde ifølge krav 1 med et isoelektrisk punkt over 5,5, fortrinsvis over 5,8, en molekylvægt på under 35 kDa, fortrinsvis på 4-30 kDa, og en glycoalkaloidkoncentration på mindre end 150 ppm.
8. Fødevareprodukt eller produkt til personlig pleje omfattende et nativt kartoffelproteinisolat ifølge et hvilket som helst af kravene 6-7.
9. Anvendelse af et nativt kartoffelproteinpatatinisolat ifølge krav 6 som et geldannelsesmiddel, et emulgeringsmiddel eller skummiddel i et fødevareprodukt eller som en kilde til farmaceutiske og terapeutiske enzymer.
10. Anvendelse af et nativt proteinproteaseinhibitorisolat ifølge krav 7 som et geldannelsesmiddel eller et skummiddel i et fødevare- eller industrielt produkt.
DK07870595.1T 2006-11-10 2007-10-25 Native kartoffelproteinisolater DK2083634T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06077000A EP1920662A1 (en) 2006-11-10 2006-11-10 Native potato protein isolates
PCT/NL2007/050513 WO2008069650A1 (en) 2006-11-10 2007-10-25 Native potato protein isolates

Publications (1)

Publication Number Publication Date
DK2083634T3 true DK2083634T3 (da) 2016-04-18

Family

ID=37896124

Family Applications (2)

Application Number Title Priority Date Filing Date
DK07103954.9T DK1974615T3 (da) 2006-11-10 2007-03-12 Proteingeldannelse
DK07870595.1T DK2083634T3 (da) 2006-11-10 2007-10-25 Native kartoffelproteinisolater

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK07103954.9T DK1974615T3 (da) 2006-11-10 2007-03-12 Proteingeldannelse

Country Status (9)

Country Link
US (4) US9102749B2 (da)
EP (3) EP1920662A1 (da)
AT (1) ATE451842T1 (da)
CA (2) CA2669096C (da)
DE (1) DE602007003805D1 (da)
DK (2) DK1974615T3 (da)
PL (2) PL1974615T3 (da)
PT (1) PT1974615E (da)
WO (1) WO2008069650A1 (da)

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Also Published As

Publication number Publication date
EP1974615A1 (en) 2008-10-01
DE602007003805D1 (de) 2010-01-28
EP1974615B1 (en) 2009-12-16
PL2083634T3 (pl) 2016-07-29
DK1974615T3 (da) 2010-04-26
US20100003394A1 (en) 2010-01-07
EP2083634B1 (en) 2016-03-30
US9968118B2 (en) 2018-05-15
EP1920662A1 (en) 2008-05-14
US9102749B2 (en) 2015-08-11
US8465911B2 (en) 2013-06-18
WO2008069650A1 (en) 2008-06-12
CA2669096C (en) 2015-06-16
EP2083634A1 (en) 2009-08-05
PT1974615E (pt) 2010-03-22
CA2669090C (en) 2014-09-09
ATE451842T1 (de) 2010-01-15
PL1974615T3 (pl) 2010-05-31
US20100040591A1 (en) 2010-02-18
US20130281669A1 (en) 2013-10-24
CA2669096A1 (en) 2008-06-12
CA2669090A1 (en) 2008-06-12
US20150257416A1 (en) 2015-09-17

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