DK200001374A - Fremgangsmåde til dannelse af non-patogen, infektiøs pancreas necrose virus (IPNV) ud fra syntetisk RNA transkripter - Google Patents
Fremgangsmåde til dannelse af non-patogen, infektiøs pancreas necrose virus (IPNV) ud fra syntetisk RNA transkripter Download PDFInfo
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- DK200001374A DK200001374A DK200001374A DKPA200001374A DK200001374A DK 200001374 A DK200001374 A DK 200001374A DK 200001374 A DK200001374 A DK 200001374A DK PA200001374 A DKPA200001374 A DK PA200001374A DK 200001374 A DK200001374 A DK 200001374A
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- necrosis virus
- pancreatic necrosis
- infectious pancreatic
- cdna
- synthetic rna
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- 241000710921 Infectious pancreatic necrosis virus Species 0.000 title description 22
- 238000000034 method Methods 0.000 title description 10
- 239000002299 complementary DNA Substances 0.000 description 22
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 12
- 241000700605 Viruses Species 0.000 description 12
- 230000001717 pathogenic effect Effects 0.000 description 12
- 239000000427 antigen Substances 0.000 description 10
- 102000036639 antigens Human genes 0.000 description 10
- 108091007433 antigens Proteins 0.000 description 10
- 229960005486 vaccine Drugs 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 4
- 230000002458 infectious effect Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 208000009663 Acute Necrotizing Pancreatitis Diseases 0.000 description 2
- 241001125819 Ameiurus nebulosus Species 0.000 description 2
- 241000711804 Infectious hematopoietic necrosis virus Species 0.000 description 2
- 241000546112 Infectious salmon anemia virus Species 0.000 description 2
- 206010058096 Pancreatic necrosis Diseases 0.000 description 2
- 241000295697 Pimephales promelas Species 0.000 description 2
- 241000711825 Viral hemorrhagic septicemia virus Species 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 241000122170 Aliivibrio salmonicida Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 241000701372 Iridovirus Species 0.000 description 1
- 241000194040 Lactococcus garvieae Species 0.000 description 1
- 241000721654 Lepomis macrochirus Species 0.000 description 1
- 241001600139 Moritella viscosa Species 0.000 description 1
- 101150076514 NS gene Proteins 0.000 description 1
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 description 1
- 208000016222 Pancreatic disease Diseases 0.000 description 1
- 241000192126 Piscirickettsia salmonis Species 0.000 description 1
- 241000186812 Renibacterium salmoninarum Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 241000544286 Vibrio anguillarum Species 0.000 description 1
- 241000607734 Yersinia <bacteria> Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10041—Use of virus, viral particle or viral elements as a vector
- C12N2720/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10061—Methods of inactivation or attenuation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
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- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
Patentkrav: 1. Fremgangsmåde til fremstilling af ikke-patogene, infektiøse pankreasenekrose-virus, hvilken fremgangsmåde omfatter følgende trin: fremstilling af et cDNA, som indeholder genom-segmenterne A og B fra infektiøs pankreasnekrose-virus, hvor cDNAet af segment A er modificeret for at forhindre ekspression af NS-protein, transkribering af cDNAet til frembringelse af syntetiske RNA-transkripter, transfektion af værtsceller med de syntetiske RNA-transkripter, inkubering af værtscellerne i et dyrkningsmedium og isolering af levende, ikke-patogene, infektiøse pankreasenekrose-virus fra dyrkningsmediet. 2. Fremgangsmåde ifølge krav l, hvorved værtscellerne er valgt fra gruppen bestående af kongelakseembryo (CHSE)-celler, regnbueørredgonade (RTG-2)-celler, Bluegill-fiske (BF-2)-celler, Brown Bullhead (BB)-celler og Fathead Minnow (FHM)-celler. 3. Fremgangsmåde ifølge krav i, hvorved cDNAet stammer fra mere end én stamme af infektiøs pankreasnekrose-virus. 4. Fremgangsmåde ifølge krav 1, hvorved cDNAet for segment A er modificeret ved hjælp af mutation af initieringskodonen for NS-genet til en stopkodon. 5. Levende, ikke-patogen, infektiøs pankreasnekrose-virus, hvor viruset er dannet ved hjælp af en proces, der omfatter trinene: fremstilling af et cDNA, som indeholder genom-segmenterne A og B fra infektiøs pankreasnekrose-virus, hvor segment A er modificeret for at forhindre ekspression af NS-protein, transkribering af cDNAet til frembringelse af et syntetisk RNA-transkript, transfektion af en værtscelle med det syntetiske RNA-transkript, inkubering af værtscellen i et dyrkningsmedium og isolering af levende, ikke-patogene, infektiøse pankreasenekrose-virus fra dyrkningsmediet. 6. cDNA, som indeholder i det mindste en del af genomet fra det infektiøse pankreasnekrose-virus, der er valgt fra gruppen bestående af segment A og segmenterne A og B, hvor cDNAet indbefatter 5'- og 3'-terminalerne af segmenterne, og hvor segment A er modificeret for at forhindre ekspression af NS-protein. 7. cDNAet ifølge krav 6, hvor cDNAet stammer fra mere end én stamme af infektiøs pankreasnekrose-virus. 8. Rekombinant vektor, som omfatter cDNAet ifølge krav 6 . 9. Vaccine, som omfatter et infektiøst, ikke-patogent pankreasnekrose-virus ifølge krav 5, hvori segment A fra viruset er modificeret i mindst to områder for at forhindre ekspression af NS-protein. 10. Fremgangsmåde til frembringelse af en vaccine af levende, ikke-patogen, infektiøs pankreasnekrose-virus, hvilken fremgangsmåde omfatter trinene: fremstilling af et uforkortet cDNA, som indeholder genomsegmenterne A og B fra infektiøs pankreasnekrose-virus, hvor segment A er modificeret for at forhindre ekspression af NS-protein, transkribering af cDNAet til frembringelse af syntetiske RNA-transkripter, transfektion af værtsceller med RNA-transkripterne, inkubering af værtscellerne i et dyrkningsmedium, isolering af levende, infektiøse, ikke-patogene pankreasnekrose-virus fra dyrkningsmediet, og blanding af de levende, ikke-patogene, infektiøse pankreasnekrose-virus med et farmaceutisk acceptabelt bæremedium til frembringelse af en vaccine med levende, ikke-patogene, infektiøse pankreasnekrose-virus. 11. Fremgangsmåde til frembringelse af et ikke-patogent, kimært gen, hvilken fremgangsmåde omfatter følgende trin: fremstilling af et cDNA, som indeholder genomsegmenterne A og B fra infektiøs pankreasnekrose-virus, hvor cDNAet af segment A er modificeret for at forhindre ekspression af NS-protein, og cDNAet af segment A koder epitopiske determinanter fra mindst to forskellige stammer af infektiøs pankreasnekrose-virus, transkribering af cDNAet til frembringelse af syntetiske RNA-transkripter, transfektion af værtsceller med de syntetiske RNA- transkripter, inkubering af værtscellerne i et dyrkningsmedium, og isolering af levende, ikke-patogene, kimære, infek-tiøse pankreasnekrose-virus fra dyrkningsmediet. 12. Ikke-patogent, kimært, akvatisk Birnavirus, hvor viruset er NS-proteindefekt og indeholder epitopiske determinanter fra mindst to forskellige Birnavirusstammer. 13. Virus ifølge krav 12, hvor det akvatiske Birnavirus er infektiøst pankreasnekrose-virus. 14. Virus ifølge krav 13, hvor de infektiøse pankreasnekrose-virusstammer er valgt fra gruppen bestående af West Buxton, Jasper, NI og SP. 15. Vaccine, som omfatter det ikke-patogene, kimære, akvatiske Birnavirus ifølge krav 12 kombineret med et farmaceutisk acceptabelt bæremedium. 16. Vaccine ifølge krav 15, hvori det akvatiske
Birnavirus er infektiøs pankreasnekrose-virus. 17. Vaccine ifølge krav 15, som yderligere omfatter antigener fra akvatiske virus, bortset fra Birnavirus, bakterielle antigener eller antigener fra akvatiske virus, bortset fra Birnavirus, i kombination med bakterielle antigener. 18. Vaccine ifølge krav 17, hvor antigenerne fra akvatiske virus, bortset fra Birnavirus, er valgt fra gruppen bestående af infektiøs, hæmatopoietisk nekrose-virus (IHNV), viral hæmoragisk septikæmi-virus (VHSV), ISAV (infektiøs lakseanæmi-virus) , PDV (pankreassygdom-svirus) , Irido-virus og Nodavirus. 19. Vaccine ifølge krav 17, hvori de bakterielle antigener er antigener fra gramnegative bakterier. 20. Vaccine ifølge krav 17, hvori de bakterielle antigener er antigener fra gramnegative bakterier. 21. Vaccine ifølge krav 17, hvori de bakterielle antigener er fra bakterier, der er valgt fra gruppen bestående af Aeroinonas salmonicida, Vibrio anguillarum, Vibrio salmonicida, Vibrio viscosus, Yersinia ruckri, Piscirickettsia salmonis, Renibacterium salmoninarum, Pasturella piscicida, Flavobacterium columnare, Flavobacterium psychrophilum og Lactococcus garvieae. 22. Syntetisk RNA, der er transkriberet fra cDNAet ifølge krav 6. 23. Værtscelle, der er transformeret med den rekombinante vektor ifølge krav 8. 24. Værtscelle, der er transformeret med et syntetisk RNA ifølge krav 22. 25. Fremgangsmåde til frembringelse af et levende, infektiøst, akvatisk Birnavirus, hvilken fremgangsmåde omfatter følgende trin: fremstilling af mindst ét cDNA af genomsegmenterne A og B fra akvatisk Birnavirus, initiering af replikation af et ds-RNA i en værtscelle ved anvendelse af RNA stammende fra cDNAet, inkubering af værtscellen i et dyrkningsmedium, og isolering af levende, infektiøse, akvatiske Birnavirus fra dyrkningsmediet. 26. Fremgangsmåde ifølge krav 25, hvorved cDNAet indeholder epitopiske determinanter fra mindst to forskellige stammer af akvatisk Birnavirus. 27. Fremgangsmåde ifølge krav 25, hvorved det akvatiske Birnavirus er infektiøs pankreasnekrose-virus.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8017898P | 1998-03-31 | 1998-03-31 | |
| PCT/US1999/004285 WO1999050419A2 (en) | 1998-03-31 | 1999-03-31 | A method for generating nonpathogenic, infectious pancreatic necrosis virus (ipnv) from synthetic rna transcripts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK200001374A true DK200001374A (da) | 2000-11-23 |
Family
ID=22155757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK200001374A DK200001374A (da) | 1998-03-31 | 2000-09-15 | Fremgangsmåde til dannelse af non-patogen, infektiøs pancreas necrose virus (IPNV) ud fra syntetisk RNA transkripter |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6274147B1 (da) |
| AU (1) | AU3353599A (da) |
| CA (1) | CA2324478C (da) |
| DK (1) | DK200001374A (da) |
| NO (2) | NO329660B1 (da) |
| WO (1) | WO1999050419A2 (da) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6596280B1 (en) * | 1997-07-31 | 2003-07-22 | University Of Maryland Biotechnology Institute | Method for generating birnavirus from synthetic RNA transcripts |
| US20040001863A1 (en) * | 1998-12-03 | 2004-01-01 | Shau-Chi Chi | Immortal cell line derived from grouper Epinephelus coioides and its applications therein |
| US6630456B2 (en) * | 1999-11-23 | 2003-10-07 | Academia Sinica | Regulation of apoptosis in aquatic organisms by aquabirnavirus |
| CA2457349A1 (en) * | 2001-08-10 | 2003-02-20 | University Of Maryland Biotechnology Institute | Sub-unit vaccine for infectious pancreatic necrosis virus |
| WO2004003143A2 (en) * | 2002-06-26 | 2004-01-08 | Advanced Bionutrition Corporation | Viruses and virus-like particles for multiple antigen and target display |
| TWI347364B (en) | 2003-02-19 | 2011-08-21 | Novartis Ag | Inactivated nodavirus vaccine |
| ES2217967B1 (es) * | 2003-03-31 | 2006-01-01 | Consejo Sup. Investig. Cientificas | Procedimiento de produccion de particulas virales vacias (vlps) del virus inductor de la bursitis infecciosa (ibdv), composiciones necesarias para su puesta a punto y su uso en la elaboracion de vacunas frente al ibdv. |
| ES2307345B1 (es) * | 2004-01-21 | 2009-11-13 | Consejo Sup. Investig. Cientificas | Capsidas vacias quimericas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones. |
| ES2307346B1 (es) * | 2004-01-21 | 2009-11-13 | Consejo Sup. Investig. Cientificas | Capsidas vacias (vlps(-vp4)) del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones. |
| EP1773858B1 (en) | 2004-05-27 | 2016-06-22 | Janssen Biotech, Inc. | Cynomolgus prostate specific antigen |
| AU2005329405A1 (en) * | 2004-06-15 | 2006-09-28 | Centocor, Inc. | Method for screening agents against human prostate disease |
| US7244432B2 (en) * | 2004-12-08 | 2007-07-17 | University Of Maryland Biotechnology Institute | Infectious bursal disease virus (IBDV) variant from Georgia |
| DE102005010288A1 (de) * | 2005-03-02 | 2006-09-07 | Riemser Arzneimittel Ag | Pharmazeutisches, oral applizierbares Präparat zur Behandlung von Fischen, Herstellungsverfahren für dieses und seine Verwendung |
| ES2310062B1 (es) * | 2005-07-15 | 2009-11-13 | Bionostra, S.L. | Particulas pseudovirales vacias quimericas derivadas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), procedimiento de obtencion y aplicaciones. |
| WO2011012999A1 (en) | 2009-07-31 | 2011-02-03 | Novartis Ag | Reverse genetics systems |
| NO332608B1 (no) * | 2011-03-16 | 2012-11-19 | Norwegian School Of Veterinary Science | Levende avirulent IPNV og vaksine for profylakse eller behandling av IPN-sykdom hos fisk |
| CN103937912B (zh) * | 2014-05-13 | 2016-07-20 | 吴斌 | 用于检测传染性胰脏坏死病毒的lamp引物组合物及其应用 |
| JP2022543423A (ja) | 2019-08-09 | 2022-10-12 | ナットクラッカー セラピューティクス， インコーポレイテッド | 治療用組成物から材料を除去するための製造方法および装置 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5788970A (en) | 1994-03-29 | 1998-08-04 | The University Of Maryland College Park | Chimeric infectious bursal disease virus CDNA clones, expression products and vaccines based thereon |
| US5871744A (en) * | 1996-09-05 | 1999-02-16 | University Of Maryland-Biotechnology Inst. | Method for generating birnavirus from synthetic RNA transcripts |
| EP0887412B1 (en) * | 1997-05-26 | 2003-10-15 | Akzo Nobel N.V. | Recombinant birnavirus vaccine |
| US6231868B1 (en) * | 1997-09-30 | 2001-05-15 | University Of Maryland-Biotechnology Institute | Method for generating nonpathogenic infections birnavirus from synthetic RNA transcripts |
-
1999
- 1999-03-31 AU AU33535/99A patent/AU3353599A/en not_active Abandoned
- 1999-03-31 CA CA2324478A patent/CA2324478C/en not_active Expired - Fee Related
- 1999-03-31 US US09/282,147 patent/US6274147B1/en not_active Expired - Fee Related
- 1999-03-31 WO PCT/US1999/004285 patent/WO1999050419A2/en active Application Filing
-
2000
- 2000-09-15 DK DK200001374A patent/DK200001374A/da not_active Application Discontinuation
- 2000-09-29 NO NO20004914A patent/NO329660B1/no not_active IP Right Cessation
-
2008
- 2008-06-16 NO NO20082682A patent/NO20082682L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| NO20004914D0 (no) | 2000-09-29 |
| US6274147B1 (en) | 2001-08-14 |
| NO20082682L (no) | 2000-11-23 |
| WO1999050419A3 (en) | 1999-11-18 |
| CA2324478A1 (en) | 1999-10-07 |
| NO329660B1 (no) | 2010-11-22 |
| NO20004914L (no) | 2000-11-23 |
| CA2324478C (en) | 2011-08-23 |
| WO1999050419A2 (en) | 1999-10-07 |
| AU3353599A (en) | 1999-10-18 |
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