DK175694B1 - Rekombinante systemer til ekspression af kolera B-underenhed ved hjælp af fremmede promotorer og/eller lederpeptider - Google Patents

Rekombinante systemer til ekspression af kolera B-underenhed ved hjælp af fremmede promotorer og/eller lederpeptider Download PDF

Info

Publication number
DK175694B1
DK175694B1 DK198904569A DK456989A DK175694B1 DK 175694 B1 DK175694 B1 DK 175694B1 DK 198904569 A DK198904569 A DK 198904569A DK 456989 A DK456989 A DK 456989A DK 175694 B1 DK175694 B1 DK 175694B1
Authority
DK
Denmark
Prior art keywords
ctb
promoter
gene
plasmid
expression
Prior art date
Application number
DK198904569A
Other languages
Danish (da)
English (en)
Other versions
DK456989D0 (da
DK456989A (da
Inventor
Jan Holmgren
Joaquin Sanches Castillo
Original Assignee
Vitec Aktiebolag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vitec Aktiebolag filed Critical Vitec Aktiebolag
Publication of DK456989D0 publication Critical patent/DK456989D0/da
Publication of DK456989A publication Critical patent/DK456989A/da
Application granted granted Critical
Publication of DK175694B1 publication Critical patent/DK175694B1/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245Escherichia (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/55Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Soy Sauces And Products Related Thereto (AREA)
  • Exhaust Gas Treatment By Means Of Catalyst (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
DK198904569A 1988-09-16 1989-09-15 Rekombinante systemer til ekspression af kolera B-underenhed ved hjælp af fremmede promotorer og/eller lederpeptider DK175694B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE8803291 1988-09-16
SE8803291A SE462285B (sv) 1988-09-16 1988-09-16 Expression av koleratoxinets bindande subenhet med hjaelp av fraemmande promotor- och/eller ledarpeptidstrukturer

Publications (3)

Publication Number Publication Date
DK456989D0 DK456989D0 (da) 1989-09-15
DK456989A DK456989A (da) 1990-03-17
DK175694B1 true DK175694B1 (da) 2005-01-24

Family

ID=20373364

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198904569A DK175694B1 (da) 1988-09-16 1989-09-15 Rekombinante systemer til ekspression af kolera B-underenhed ved hjælp af fremmede promotorer og/eller lederpeptider

Country Status (13)

Country Link
EP (1) EP0368819B1 (no)
JP (1) JP2790333B2 (no)
AT (1) ATE161885T1 (no)
AU (1) AU633842B2 (no)
CA (1) CA1340577C (no)
DE (1) DE68928529T2 (no)
DK (1) DK175694B1 (no)
ES (1) ES2113342T3 (no)
FI (1) FI104496B (no)
GR (1) GR3025846T3 (no)
IS (1) IS1804B (no)
NO (1) NO301175B1 (no)
SE (1) SE462285B (no)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416505A3 (en) * 1989-09-04 1991-10-02 Takeda Chemical Industries, Ltd. Expression plasmids and use thereof
US5356797A (en) * 1991-11-15 1994-10-18 Board Of Regents, The University Of Texas Membrane expression of heterologous genes
EP0613500A1 (en) * 1991-11-15 1994-09-07 The Board Of Regents, The University Of Texas System Membrane expression of heterologous genes
US5932714A (en) * 1995-02-23 1999-08-03 Connaught Laboratories Limited Expression of gene products from genetically manipulated strains of Bordetella
US5998168A (en) * 1995-06-07 1999-12-07 Connaught Laboratories Limited Expression of gene products from genetically manipulated strains of bordetella
US6008329A (en) * 1998-03-06 1999-12-28 The United States Of America As Represented By The Secretary Of The Army Method for purifying cholera toxin
WO2007117339A2 (en) * 2006-01-11 2007-10-18 The United States Of America As Respresented By The Secretary Of The Navy Adhesin-enterotoxin chimera based immunogenic composition against entertoxigenic escherichia coli
DE102011111688A1 (de) * 2011-08-24 2013-02-28 Bioserv Analytik Und Medizinprodukte Gmbh Verfahren zur schnellen Isolation bzw. Aufreinigung von rekombinanten Proteinen mit einer (nicht toxischen) Choleratoxin-Domäne
CN109096403B (zh) * 2018-07-04 2020-06-30 浙江工商大学 一种用于蛋白质转导的蛋白质载体及其制备方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666837A (en) * 1982-05-24 1987-05-19 Smithkline-Rit DNA sequences, recombinant DNA molecules and processes for producing the A and B subunits of cholera toxin and preparations containing so-obtained subunit or subunits
US5135862A (en) * 1983-03-04 1992-08-04 University Of Maryland Method of isolating restriction fragment deletions in vibrio cholerae, products thereof
US4882278A (en) * 1983-04-29 1989-11-21 President And Fellows Of Harvard College Non-toxinogenic vibrio cholerae mutants
EP0251579A3 (en) * 1986-06-24 1989-03-22 Enterovax Research Pty. Ltd. Non-antibiotic marker system
EP0358692B1 (en) * 1987-04-29 1998-06-03 President And Fellows Of Harvard College Cholera vaccines

Also Published As

Publication number Publication date
SE462285B (sv) 1990-05-28
ATE161885T1 (de) 1998-01-15
NO893702L (no) 1990-03-19
EP0368819B1 (en) 1998-01-07
FI894368A (fi) 1990-03-17
ES2113342T3 (es) 1998-05-01
JP2790333B2 (ja) 1998-08-27
NO893702D0 (no) 1989-09-15
FI894368A0 (fi) 1989-09-15
CA1340577C (en) 1999-06-01
DE68928529T2 (de) 1998-04-16
IS3505A7 (is) 1990-03-17
NO301175B1 (no) 1997-09-22
DK456989D0 (da) 1989-09-15
DE68928529D1 (de) 1998-02-12
EP0368819A1 (en) 1990-05-16
GR3025846T3 (en) 1998-04-30
AU633842B2 (en) 1993-02-11
DK456989A (da) 1990-03-17
IS1804B (is) 2002-02-26
JPH0322997A (ja) 1991-01-31
SE8803291D0 (sv) 1988-09-16
AU4134989A (en) 1990-04-05
FI104496B (fi) 2000-02-15

Similar Documents

Publication Publication Date Title
Sanchez et al. Recombinant system for overexpression of cholera toxin B subunit in Vibrio cholerae as a basis for vaccine development.
JP5566684B2 (ja) Clostridiumdifficileに対する、組換え毒素A/毒素Bワクチン
US5589384A (en) Fusion proteins
Sanchez et al. Genetic fusion of a non‐toxic heat‐stable enterotoxin‐related decapeptide antigen to cholera toxin B‐subunit
Dertzbaugh et al. Comparative effectiveness of the cholera toxin B subunit and alkaline phosphatase as carriers for oral vaccines
Butterton et al. Coexpression of the B subunit of Shiga toxin 1 and EaeA from enterohemorrhagic Escherichia coli in Vibrio cholerae vaccine strains
US20050244425A1 (en) Histidine-tagged intimin and methods of using intimin to stimulate an immune response and as an antigen carrier with targeting capability
CA2413045A1 (en) Expression system
US5268276A (en) Recombinant systems for expression of cholera B-sub-unit with the aid of foreign promoters and/or leader peptides
Byun et al. Nasal immunization with E. coli verotoxin 1 (VT1)-B subunit and a nontoxic mutant of cholera toxin elicits serum neutralizing antibodies
Kazemi et al. Immunogenic properties of trivalent recombinant protein composed of B-subunits of LT, STX-2, and CT toxins
DK175694B1 (da) Rekombinante systemer til ekspression af kolera B-underenhed ved hjælp af fremmede promotorer og/eller lederpeptider
US5834246A (en) Recombinant systems for expression of cholera B-subunit with the aid of foreign promoters and/or leader peptides
EP0168322A2 (en) Production of the E. coli LT-B enterotoxin subunit
Hirst et al. Biogenesis of cholera toxin and related oligomeric enterotoxins
US6043057A (en) Recombinant systems for expression of the cholera B-sub-unit with the aid of foreign promoters and/or leader peptides
Sirard et al. A recombinant Bacillus anthracis strain producing the Clostridium perfringens Ib component induces protection against iota toxins
CA2366462C (en) Two-step immunization procedure against chlamydia infection
Lim et al. Expression of functional pentameric heat-labile enterotoxin B subunit of Escherichia coli in Saccharomyces cerevisiae
US20070212757A1 (en) Expression System for the B Subunit of Cholera Toxin
Yakhchali Expression of foreign epitopes in CS3 fimbriae
Singh et al. Production, Purification and Characterization of Chicken Egg Yolk Monoclonal Antibody Against Colonization factor antigen-1 of Enterotoxigenic Escherichia coli Causing Diarrhea
Bouzari et al. Construction and Expression of a Fused Gene for B Subunit of the Heat-Labile and a Truncated Form of the Heat-Stable Enterotoxins in Escherichia coli
Lipscombe Construction and characterisation of" Escherichia coli" heat-labile toxin B-subunit fusion proteins
Judge The author hereby certifies that the use of any copyrighted material in the thesis manuscript entitled:“Toward Development of an Oral, Plant-based Vaccine Against Escherichia coli O157: H7.”

Legal Events

Date Code Title Description
PUP Patent expired