DK173650B1 - Fremgangsmåde til gennaturering af proteiner - Google Patents

Fremgangsmåde til gennaturering af proteiner Download PDF

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Publication number
DK173650B1
DK173650B1 DK198701797A DK179787A DK173650B1 DK 173650 B1 DK173650 B1 DK 173650B1 DK 198701797 A DK198701797 A DK 198701797A DK 179787 A DK179787 A DK 179787A DK 173650 B1 DK173650 B1 DK 173650B1
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Prior art keywords
protein
concentration
reactivation
denatured
solution
Prior art date
Application number
DK198701797A
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Danish (da)
English (en)
Other versions
DK179787D0 (da
DK179787A (da
Inventor
Rainer Rudolph
Stephan Fischer
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of DK179787D0 publication Critical patent/DK179787D0/da
Publication of DK179787A publication Critical patent/DK179787A/da
Application granted granted Critical
Publication of DK173650B1 publication Critical patent/DK173650B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK198701797A 1986-04-08 1987-04-08 Fremgangsmåde til gennaturering af proteiner DK173650B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19863611817 DE3611817A1 (de) 1986-04-08 1986-04-08 Verfahren zur renaturierung von proteinen
DE3611817 1986-04-08

Publications (3)

Publication Number Publication Date
DK179787D0 DK179787D0 (da) 1987-04-08
DK179787A DK179787A (da) 1987-10-09
DK173650B1 true DK173650B1 (da) 2001-05-21

Family

ID=6298240

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198701797A DK173650B1 (da) 1986-04-08 1987-04-08 Fremgangsmåde til gennaturering af proteiner

Country Status (20)

Country Link
US (1) US4933434A (cs)
EP (1) EP0241022B1 (cs)
JP (1) JPH0742307B2 (cs)
KR (1) KR900005279B1 (cs)
AT (1) ATE84541T1 (cs)
AU (1) AU583723B2 (cs)
CA (1) CA1304191C (cs)
CZ (1) CZ283304B6 (cs)
DD (1) DD260280A5 (cs)
DE (2) DE3611817A1 (cs)
DK (1) DK173650B1 (cs)
ES (1) ES2038969T3 (cs)
FI (1) FI93842C (cs)
GR (1) GR3007605T3 (cs)
HK (1) HK46896A (cs)
HU (1) HU198514B (cs)
IE (1) IE60064B1 (cs)
IL (1) IL82091A (cs)
SK (1) SK249587A3 (cs)
ZA (1) ZA872474B (cs)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001484A1 (en) * 1987-08-14 1989-02-23 Gebro Broschek Kg Pharmazeutische Fabrik Process for intramolecular oxidation of two -sh groups in a peptide compound to a disulphide bridge, and peptide so obtained
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
EP0545999B1 (en) * 1990-08-20 1997-06-04 Novo Nordisk A/S Process for the preparation of biologically active IGF-1 using IGF-1 having an amino-terminal extension
ES2119779T3 (es) * 1990-09-05 1998-10-16 Southern Cross Biotech Pty Ltd Solubilizacion de proteinas en formas activas.
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4139000A1 (de) 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
EP1077263A1 (de) 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
US20050079526A1 (en) * 2002-02-20 2005-04-14 Affinium Pharmaceuticals, Inc. Methods and apparatuses for characterizing refolding and aggregation of biological molecules
WO2011113601A1 (en) 2010-03-17 2011-09-22 Biogenerix Ag Method for obtaining biologically active recombinant human g-csf
WO2012104099A1 (en) 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN106699836A (zh) * 2017-03-07 2017-05-24 安徽鑫华坤生物工程有限公司 一种蛋白质复性的透析设备

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4216141A (en) 1978-07-19 1980-08-05 The Salk Institute For Biological Studies Method for cyclization of peptides
DE3231658A1 (cs) 1981-01-21 1983-11-17
EP0112849B1 (en) * 1982-06-07 1987-09-16 Celltech Limited A process for the preparation of chymosin
JPS593711U (ja) * 1982-06-30 1984-01-11 協伸工業株式会社 電線被覆剥取器
AU538170B2 (en) * 1982-09-15 1984-08-02 Owens-Illinois Glass Container Inc. Modified protein to mould an enzyme type molecule
GR79124B (cs) * 1982-12-22 1984-10-02 Genentech Inc
GB2138004B (en) * 1983-03-25 1987-05-13 Celltech Ltd A process for the production of a protein
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
KR860007688A (ko) 1985-03-08 1986-10-15 시끼 모리야 전자석장치(電磁石裝置)
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
EP0208539A3 (en) * 1985-07-11 1988-08-03 Repligen Corporation Folding disulfide-cross-linkable proteins
US4656255A (en) * 1985-09-09 1987-04-07 International Minerals & Chemical Corp. Protein recovery
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form

Also Published As

Publication number Publication date
ES2038969T3 (es) 1993-08-16
AU583723B2 (en) 1989-05-04
EP0241022A2 (de) 1987-10-14
HUT44046A (en) 1988-01-28
CZ249587A3 (en) 1997-11-12
IE870695L (en) 1987-10-08
DK179787D0 (da) 1987-04-08
DE3611817A1 (de) 1987-10-15
ZA872474B (en) 1988-07-27
FI93842B (fi) 1995-02-28
FI93842C (fi) 1995-06-12
FI871522A0 (fi) 1987-04-07
HK46896A (en) 1996-03-22
JPS62242697A (ja) 1987-10-23
GR3007605T3 (cs) 1993-08-31
CA1304191C (en) 1992-06-23
DD260280A5 (de) 1988-09-21
JPH0742307B2 (ja) 1995-05-10
ATE84541T1 (de) 1993-01-15
IE60064B1 (en) 1994-06-01
IL82091A0 (en) 1987-10-30
CZ283304B6 (cs) 1998-02-18
US4933434A (en) 1990-06-12
HU198514B (en) 1989-10-30
FI871522A (fi) 1987-10-09
DK179787A (da) 1987-10-09
IL82091A (en) 1991-12-15
SK279976B6 (sk) 1999-06-11
EP0241022A3 (en) 1989-10-25
EP0241022B1 (de) 1993-01-13
DE3783497D1 (de) 1993-02-25
KR870010185A (ko) 1987-11-30
KR900005279B1 (ko) 1990-07-27
SK249587A3 (en) 1999-06-11
AU7119087A (en) 1987-10-15

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Legal Events

Date Code Title Description
B1 Patent granted (law 1993)