DK144113B - GROWTH PROMOTING FEED AND ADDITIVE TO USE IN FEED - Google Patents
GROWTH PROMOTING FEED AND ADDITIVE TO USE IN FEED Download PDFInfo
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- DK144113B DK144113B DK258373AA DK258373A DK144113B DK 144113 B DK144113 B DK 144113B DK 258373A A DK258373A A DK 258373AA DK 258373 A DK258373 A DK 258373A DK 144113 B DK144113 B DK 144113B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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Description
U4113 iU4113 i
Den foreliggende opfindelse angår et vækstfremmende foder samt et additiv til anvendelse i foderet.The present invention relates to a growth-promoting feed as well as an additive for use in the feed.
Kalveopdræt og kalveopfedning har en voksende betydning under de ændrede spisevaner af store dele af befolkningen, dvs. fore-5 trækkeisen af fedtfattige og let fordøjelige kødprodukter.Calf breeding and calf fattening have a growing importance under the changing eating habits of large sections of the population, ie. preferring the high density of low fat and easily digestible meat products.
Forudsætningen for økonomien i opdræt og opfedning er ved siden af opnåelse af en høj kødkvalitet en god foderudnyttelse og et mindst muligt tab af dyr.In addition to achieving high meat quality, the prerequisite for the economy of breeding and fattening is good feed utilization and the least possible loss of animals.
Trods de i de sidste år forbedrede opholds- og fodringsbe-10 tingelser, er kalvetab stadig betydelige. Dette skyldes dels infektiøse sygdomme, men er for en stor del også fodringsbetinget. Tabene, der forårsages af infektiøse sygdomme, skal imødegås ved hygiejniske foranstaltninger, såsom anbringelse af dyrene i enkeltbokse, regelmæssig desinfektion af stald 15 og dyr, samt eventuel indgift af medikamenter. For så vidt angår fodringsbetingede tab er iværksættelse af virksomme modforanstaltninger væsentligt vanskeligere, da sammenhængen her er meget vanskelige at gennemskue, og virkningerne fra dyr til dyr er meget forskellige. En meget stor procentsats, 20 ca. halvdelen af de samlede tab ved opdræt og opfedning, kan føres tilbage til uspecifikke enteritider. Men også selv om diarreerne ikke altid fører til død, betyder de i hvert tilfælde et stærkt tilbagefald i udviklingen af dyrene, som kan stille et spørgsmålstegn ved økonomien i den samlede opdræt 25 og opfedning.Despite the improved living and feeding conditions in recent years, calf loss is still considerable. This is partly due to infectious diseases, but is also largely dependent on feeding. The losses caused by infectious diseases must be countered by hygienic measures, such as placing the animals in single boxes, regular disinfection of stables 15 and animals, as well as possible administration of medication. As far as feeding-related losses are concerned, effective countermeasures are considerably more difficult, since the context here is very difficult to understand and the effects from animal to animal are very different. A very large percentage, 20 approx. half of the total losses on breeding and fattening can be traced back to nonspecific enteritis times. But even if the diarrhea does not always lead to death, in each case they mean a sharp decline in the development of the animals, which may question the economy of the total breeding 25 and fattening.
Fra beskrivelsen til tysk fremlæggelsesskrift nr. 1.222.361 er det kendt at anvende et forgæringsprodukt af Bacillus licheniformis scm vækstfremmende tilsætning til foder. Bacillus liche= niformis danner foruden det vækstfremmende stof også antibiotikumet 30 Bacitracin, som må fjernes ved opløsningsmiddelekstraktion, idet det er U4113 2 uønsket at have antibiotika i foder.It is known from the description of German Patent Specification No. 1,222,361 to use a fermentation product of Bacillus licheniformis scm growth-enhancing feed additive. In addition to the growth promoter, Bacillus liche = niformis also produces the antibiotic 30 Bacitracin, which must be removed by solvent extraction, as it is undesirable to have antibiotics in feed.
Den foreliggende opfindelse er baseret på anvendelse af et vækstfremmende stof dannet af den til arten Bacillus licheni= formis hørende stamme P 30¾ som ikke har den ulempe at dan-5 ne Bacitracin.The present invention is based on the use of a growth promoter formed from the strain of Bacillus licheni = formis strain P30¾ which does not have the disadvantage of forming Bacitracin.
Det vækstfremmende foder ifølge opfindelsen er ejendommeligt ved, at det ud over sædvanlige foderbestanddele indeholder et forgæringsprodukt udvundet ved dyrkning af den til arten Bacillus licheniformis hørende stamme P 300 i et kulturme-10 dium og fraskillelse fra næringsmediet og fra cellematerialet.The growth-promoting feed according to the invention is characterized in that it contains, in addition to the usual feed ingredients, a fermentation product obtained by growing the strain belonging to the species Bacillus licheniformis P 300 in a culture medium and separation from the nutrient medium and from the cell material.
Den variant af Bacillus licheniformis, stammen P 300, som blev benyttet ifølge opfindelsen til udvinding af det anvendte forgæringsprodukt, er under nummeret OR 48 deponeret i Labo-15 ratorium voor Microbiologie, Microbenverzameling, Delft Hol-lån, Julianalaan 67 A, den 23.4.1971.The variant of Bacillus licheniformis, the strain P 300 used according to the invention to recover the fermentation product used, is deposited under the number OR 48 in the Laboratory of Microbiology, Microbenverzameling, Delft Hol loan, Julianalaan 67 A, 23.4. 1,971th
De morphologiske og stofskiftefysiologiske egenskaber af bakterien P 300 blev undersøgt og til bestemmelse af slægt og art især sammenlignet med angivelserne "Bergey's Manual of 20 Determinative Bacteriology". Herved blev der for de fleste egenskabers vedkommende konstateret vidtgående lighed med arten Bacillus licheniformis. Nærmere angivelser over de udførte prøver kan ses i tysk patentansøgning P 20 63 988.7 (DOS 2.063.988) .The morphological and metabolic properties of the bacterium P 300 were investigated and to determine genus and species in particular compared to the "Bergey's Manual of 20 Determinative Bacteriology". Thus, for most of the characteristics, far-reaching similarities with the species Bacillus licheniformis were found. Details of the tests performed can be found in German patent application P 20 63 988.7 (DOS 2,063,988).
25 Fremgangsmåden til fremstilling af det ifølge opfindelsen anvendte forgæringsprodukt kan udføres i et flydende eller fast næringsmedium, idet det flydende næringsmedium i almindelighed foretrækkes. Ved dyrkning i en næringsopløsning arbejdes efter den sædvanlige rystekultursmetode eller forgæ-30 ringsmetode.The process for preparing the fermentation product of the invention can be carried out in a liquid or solid nutrient medium, with the liquid nutrient medium generally being preferred. When cultivated in a nutrient solution, work according to the usual shaking culture method or fermentation method.
Næringsmediet, der skal anvendes til dyrkningen, fremstilles U 4113 3 på sædvanlig måde, og skal indeholde en kulstofkilde, en nitrogenkilde og andre for mikroorganismen nødvendige næringsstoffer og vækststoffer. Som egnede kulstofkilder kan nævnes stivelse, dextrin, rørsukker, glukose, fruktose, maltose og 5 sukkerholdige affaldsstoffer. Som nitrogenkilder kan der være tale om ammoniumsalte, urinstof, kasein, gelatine, majsstøbevand og sojabønnemel eller -kager. Endvidere kan der tilsættes uorganiske salte som f.eks. natrium-, kalium-, am= moniumhydrogenphosphater, calcium- og magniumsalte. Endvidere 10 kan det være fordelagtigt til næringsmediet at sætte forgær ingsf remskyndere , såsom gærekstrakt og vitaminer.The nutrient medium to be used in the culture is prepared in the usual manner and must contain a carbon source, a nitrogen source and other nutrients and growth substances necessary for the microorganism. Suitable carbon sources include starch, dextrin, cane sugar, glucose, fructose, maltose and 5 sugary wastes. Nitrogen sources may include ammonium salts, urea, casein, gelatin, maize cast water and soybean meal or cakes. In addition, inorganic salts such as e.g. sodium, potassium, am = monium hydrogen phosphates, calcium and magnesium salts. Furthermore, it may be advantageous for the nutrient medium to add fermentation accelerators such as yeast extract and vitamins.
Forgæringstemperaturen kan ligge inden for grænserne 25 til 55°C, men er fortrinsvis mellem 35 og 40°C. pH-værdien af næringsmediet kan være 5,0 til 9,0, fortrinsvis 6,5 til 8,0.The fermentation temperature may be within the limits of 25 to 55 ° C, but is preferably between 35 and 40 ° C. The pH of the nutrient medium may be 5.0 to 9.0, preferably 6.5 to 8.0.
15 Dyrkningen udføres i almindelighed i en tid fra 20 til 72 timer.Cultivation is generally carried out for a time from 20 to 72 hours.
Det ifølge ovenstående ved dyrkningen fremkomne forgæringsprodukt kan udfældes af den filtrerede eller centrifugerede næringsopløsning på kendte måder ved tilsætning af organiske 20 opløsningsmidler eller ved udsaltning med f.eks. natrium= chlorid, natriumsulfat, ammoniumsulfat eller calciumchlorid og koncentreres.The fermentation product obtained according to the above cultivation may be precipitated by the filtered or centrifuged nutrient solution in known ways by the addition of organic solvents or by salting with e.g. sodium = chloride, sodium sulfate, ammonium sulfate or calcium chloride and concentrated.
Fremstillingen af forgæringsproduktet kan f.eks. udføres på den i det følgende beskrevne måde.The preparation of the fermentation product may e.g. is performed in the manner described below.
25 Gæringsforsøget blev udført i en 15 liter forgæringsbeholder med 10 liter næringsmedium. Til fremstilling af næringsmediet blev 1000 g kartoffelstivelse nedbrudt af amylase, 250 g sojabønnemel, 100 g kasein, 50 g gelatine, 100 g majsstøbevand, 150 g 5 g magniumsulfat suspenderet i 30 ledningsvand og fyldt op til 10 liter. Næringsmediets pH- værdi blev med fortyndet natronlud indstillet på 7,2. Efter sterilisation var opløsningens pH-værdi 6,5. Efter podning af næringsmediet med Bacillus P 300 blev der dyrket i 50 144113 4 timer ved 39°C. Herunder blev der med henblik på gennemluft-ning indledt 10 liter luft pr. minut, og omrøringshastigheden var 300 omdrejninger pr. minut. Efter afslutning af dyrkningen blev forgæringsbeholderens indhold centrifugeret. Med 5 henblik på videre oparbejdning blev der til 5 liter af den centrifugerede forgæringsvæske sat 100 g krystallinsk cal= ciumchlorid, og der blev indstillet til en pH-værdi på 7,5 og filtreret. Filtratet blev inddampet i vakuum til 1,7 liter. Ved langsom tilsætning af 425 g tørret natriumsulfat, 10 blev der af den inddampede opløsning udfældet forgæringsproduktet, som blev fraskilt ved filtrering. Af de 5 liter centrifugeret væske fremkom 98 g af forgæringsproduktet i pulverform. Det på denne måde fremkomne forgæringsprodukt kan anvendes i denne form eller i konfektioneret form som 15 additiv til foder.The fermentation test was carried out in a 15 liter fermentation vessel with 10 liters of nutrient medium. To prepare the nutrient medium, 1000 g of potato starch was digested by amylase, 250 g of soybean flour, 100 g of casein, 50 g of gelatin, 100 g of maize cast water, 150 g of 5 g of magnesium sulfate suspended in 30 tap water and filled up to 10 liters. The pH of the nutrient was adjusted to 7.2 with dilute baking soda. After sterilization, the pH of the solution was 6.5. After inoculation of the nutrient medium with Bacillus P 300, 50 hours were cultivated for 4 hours at 39 ° C. Below, for the purpose of aeration, 10 liters of air per unit of air was introduced. and the stirring speed was 300 rpm. minute. After completion of cultivation, the fermentation vessel contents were centrifuged. For further processing, 100 g of crystalline calcium chloride was added to 5 liters of the centrifuged fermentation liquid and adjusted to a pH of 7.5 and filtered. The filtrate was evaporated in vacuo to 1.7 liters. By slowly adding 425 g of dried sodium sulfate, 10 of the evaporated solution precipitated the fermentation product which was separated by filtration. Of the 5 liters of centrifuged liquid, 98 g of the fermentation product was obtained in powder form. The fermentation product thus obtained can be used in this form or in a ready-made form as a feed additive.
Andre lignende kulturforanstaltninger og oparbejdningsfor-an'staltninger kan anvendes med lignende resultat.Other similar cultural and reprocessing measures may be used with similar results.
Ifølge opfindelsen er indholdet af forgæringsproduktet i foderet fortrinsvis 0,001 til 1,0 vægt%, og i et foder ifølge 20 opfindelsen til opfedning af kalve er indholdet af forgæringsproduktet hensigtsmæssigt 0,01 til 0,5 vægt%.According to the invention, the content of the fermentation product in the feed is preferably 0.001 to 1.0% by weight, and in a feed according to the invention for fattening calves, the content of the fermentation product is preferably 0.01 to 0.5% by weight.
Opfindelsen angår endvidere et additiv til anvendelse i et vækstfremmende foder, hvilket additiv er ejendommeligt ved, at det består af et forgæringsprodukt udvundet ved dyrkning 25 af den til arten Bacillus licheniformis hørende stamme P 300 i et kulturmedium og fraskillelse fra næringsmediet og fra cellematerialet.The invention further relates to an additive for use in a growth-promoting feed which is characterized in that it consists of a fermentation product obtained by growing the strain P 300 of the species Bacillus licheniformis in a culture medium and separating it from the nutrient medium and from the cell material.
Additivet ifølge opfindelsen kan hensigtsmæssigt foreligge i form af et pulver, i et af næringssalte omhyllet granulat el-30 ler i en på sædvanlig måde indkapslet form, idet disse former let blandes med fodermidler.Conveniently, the additive according to the invention may be in the form of a powder, in a granular envelope of nutrients or in a conventionally encapsulated form, these forms being readily admixed with feed materials.
Man kan også hos fabrikanten konfektionere fødemidlet med 5You can also confiscate the food with the manufacturer by 5
UiU 1 13 forgæringsproduktet, hvorved der ligeledes skal anvendes en mængde på 0,001 til 1,0 vægt%, beregnet som forgæringsprodukt, beregnet på det samlede fodermiddel. Herved kan forgæringsproduktet blandes med det øvrige fodermiddel i omhyl- 5 let granulatform eller i indkapslet form, idet der til omhyl-ningen kan anvendes de iøvrigt i fodermidlet indeholdte salte eller sukkerarter og til indkapslingen kan anvendes gelatine, cellulose eller stivelsederivater og vandopløselige polymere.The fermentation product, which also uses an amount of 0.001 to 1.0% by weight, calculated as the fermentation product, calculated on the total feed. Hereby, the fermentation product can be mixed with the other feed material in wrapped granular or encapsulated form, the other salts or sugars contained in the feed agent being used for the wrapping and gelatin, cellulose or starch derivatives and water-soluble polymers for the encapsulation.
10 I specielle tilfælde er det også muligt i stedet for det pulverformige forgæringsprodukt at anvende et flydende koncentrat, således som det f.eks. kan fås ved vidtgående ind-dampning af det ved fremstillingen fremkomne filtrat til blanding med de fodermidler, som dyrene skal have.In special cases, it is also possible to use a liquid concentrate instead of the powdery fermentation product, such as e.g. can be obtained by evaporating the filtrate obtained in preparation for mixing with the feed materials which the animals must have.
15 Til fodringsforsøgene blev anvendt i alt 27 kalve, som var opdelt i tre grupper på hver 9 dyr. De købte dyr var ca. 2 uger gamle, og havde vægte, der lå fra 38 til 56 kg, og var alle i god sundhedstilstand. De var indtil dette tidspunkt opfodret med Colostralmælk eller sødmælk.A total of 27 calves were used for the feeding experiments, which were divided into three groups of 9 animals each. The animals purchased were approx. 2 weeks old and had weights ranging from 38 to 56 kg and were all in good health. Up to this point they had been fed colostral milk or whole milk.
20 For at sikre en gnidningsløs overgang i fodringsforsøget blev alle dyrene underkastet en engangsprofylaktisk behandling med vitaminer, et antibiotikum til stresskompensering og et kredsløbunderstøttende middel, således som dette er almindeligt.20 In order to ensure a smooth transition in the feeding trial, all animals were subjected to disposable prophylactic treatment with vitamins, an antibiotic for stress compensation and a circuit-assisting agent, as is common.
25 Boksene til anbringelse af dyrene blev grundigt rensede og desinficerede, og der blev draget omsorg for en tilfredsstillende klimaregulering i stalden under fodringsforsøget ved opvarmning og ventilation. Ved omhyggelig overvågning af hygiejneforanstaltninger blev der skabt sikkerhed for, 30 at omgivelsernes betingelser for alle tre dyregrupper var ens. Således blev drikkespandene efter hvert måltid skyllet og desinficeret. Gangen foran boksene blev efter hver fodring renset og desinficeret. Over kalvene blev der dagligt 144113 6 sprøjtet et i handelen værende stalddesinfektionsmiddel (handelsprodukt VL - Incidin 02) i en 0,5%'ig koncentration.25 The boxes for placing the animals were thoroughly cleaned and disinfected, and a satisfactory climate control was provided in the barn during the feeding and heating experiments. Careful monitoring of hygiene measures ensured that the ambient conditions of all three animal groups were similar. Thus, after each meal, the drinking buckets were rinsed and disinfected. The hallway in front of the boxes was cleaned and disinfected after each feeding. A commercial disinfectant (commercial product VL - Incidin 02) was sprayed over the calves at a 0.5% concentration daily.
Når der optrådte diarré, blev de pågældende bokse grundigt renset med et desinficerende rensningsmiddel (handelsprodukt 5 P 3 - asepto aktiv) og derpå endnu engang desinficeret med det i handelen værende stalddesinfektionsmiddel. Også udrøringsbeholderne til det til fodringen anvendte mælkeerstatningsfoder blev efter hver benyttelse udvasket med en varm opløsning af det desinficerende rensemiddel. Åbnede fodersæk-10 ke blev efter hver udtagelse af mælkeerstatningspulveret lukket igen omhyggeligt.When diarrhea occurred, the boxes in question were thoroughly cleaned with a disinfectant cleaner (commercial product 5 P 3 - asepto active) and then disinfected again with the commercially available disinfectant. Also, the agitation containers for the milk substitute feed used for feeding were washed after each use with a warm solution of the disinfectant cleaner. Opened liner bags after each withdrawal of the milk substitute powder were closed again carefully.
Til fodring af alle tre dyregrupper blev anvendt et i handelen værende mælkeerstatningsfoder, som i det væsentlige bestod af skummetmælkspulver, forskellige fedtbestanddele, for-15 sukret majsstivelse, mineraltiIsætninger og vitamintilsætninger og som ved fin fordeling i varmt vand straks gav en brugbar drik. Endvidere blev der til dyrene i alle tre grupper til det første måltid indrørt to spiseskefulde "Neydhar-tinger Heilmoor" i drikken. (Neydhartinger Heilmoor er et re-20 gistreret varemærke for et præparat udvundet af naturlig mose-jord, som bruges til regulering af dyrenes tarmfunktion).For the feeding of all three animal groups, a commercially available milk substitute feed was used, consisting essentially of skimmed milk powder, various fat ingredients, sugared corn starch, mineral supplements and vitamin supplements, and which, by fine distribution in hot water, immediately yielded a usable drink. Furthermore, for the animals in all three groups, for the first meal, two tablespoons of "Neydhar-tinger Heilmoor" were added to the drink. (Neydhartinger Heilmoor is a registered trademark of a preparation derived from natural marsh soil used to regulate the intestinal function of animals).
Kontrolgruppen på 9 dyr fik under fodringsforsøget det i handelen værende mælkeerstatningsfoder uden forgæringsprodukt.During the feeding trial, the control group of 9 animals received the milk replacer feed without fermentation product.
Gruppe 1 på 9 dyr fik en drik, hvori der beregnet på mælke-25 erstatningsfoder, var iblandet 0,07% af additivet ifølge opfindelsen. I drikken til de 9 dyr i gruppe 2, var der beregnet på mælkeerstatningsfoder 0,12% af additivet ifølge opfindelsen.Group 1 of 9 animals was given a beverage which, based on milk substitute feed, contained 0.07% of the additive of the invention. In the drink for the 9 animals in group 2, 0.12% of the additive according to the invention was calculated on milk substitute feed.
Efter tre måneders forsøgsfodring på den ovenfor beskrevne 30 måde, kunne der gives følgende bedømmelse af dyrene i de tre grupper.After three months of experimental feeding in the manner described above, the following assessment of the animals in the three groups could be given.
I kontrolgruppen, der var blevet fodret med et mælkeerstatningsfoder uden iblanding af forgæringsproduktet ifølge op- 7 1441 13 findelsen, havde alle 9 dyr gennem et tidsrum på ca. 3 uger diarrétilfælde. Sammenlignet med dyrene i gruppe 1 og 2, er dyrene i kontrolgruppen synligt tilbagestående i udviklingen, og også i den ydre habitus, såsom glathed og glans af pelsen 5 er dyrene i gruppe 1 og 2 kontrolgruppen tydeligt overlegne.In the control group, which had been fed a milk substitute feed without the incorporation of the fermentation product according to the invention, all 9 animals had for a period of approx. 3 weeks of diarrhea. Compared to the animals in groups 1 and 2, the animals in the control group are visibly lagging in development, and also in the outer habitus, such as smoothness and gloss of the fur 5, the animals in groups 1 and 2 control group are clearly superior.
I grupperne 1 og 2 har dyrene en glat og skinnende pels og ser ved første blik meget bedre og kraftigere ud end dyrene i kontrolgruppen. Nævneværdige forskelle mellem dyrene i gruppe 1 og gruppe 2 kunne derimod ikke konstateres.In groups 1 and 2 the animals have a smooth and shiny coat and at first glance looks much better and more powerful than the animals in the control group. However, no significant differences between the animals in group 1 and group 2 were found.
10 De ovenfor beskrevne fodringsforsøg har vist, at opdræt og opfedning af kalve kan udføres mere økonomisk ved iblanding af additivet til det sædvanlige foder. Som foretrukket mængdeområde af additivet beregnet på det samlede fodermiddel, anses området fra 0,01 til 0,5 vægt% beregnet som forgærings-15 produkt til fodermidler til opdræt og opfedning af kalve.10 The feeding tests described above have shown that rearing and fattening calves can be performed more economically by admixture of the additive to the usual feed. As the preferred amount range of the additive calculated on the total feed, the range of 0.01 to 0.5% by weight calculated as fermentation product for feed materials for rearing and fattening calves is considered.
Selv om additivet ifølge opfindelsen i første række er af betydning til opdræt og opfedning af kalve, kan det også med godt resultat anvendes til fodring af andre husdyr, som f.eks, fjerkræ.Although the additive according to the invention is primarily important for rearing and fattening calves, it can also be used with good results for feeding other livestock, such as poultry.
20 I det følgende er vist resultaterne af fodringsforsøg med fedekalve, grise og foreller under anvendelse af P 300-protease. De anførte mængder er beregnet på proteaseforblanding, som havde følgende sammensætning: 77% skummetmælkspulver 25 9% sojaolie 9% P 300-protease 5% natriumsulfat20 The following is shown the results of feeding experiments with fat calves, pigs and trout using P 300 protease. The amounts indicated are for protease premix having the following composition: 77% skimmed milk powder 25 9% soybean oil 9% P 300 protease 5% sodium sulfate
Claims (6)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2225363A DE2225363C3 (en) | 1972-05-25 | 1972-05-25 | Feed for poultry and for rearing and fattening calves |
| DE2225363 | 1972-05-25 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK144113B true DK144113B (en) | 1981-12-14 |
| DK144113C DK144113C (en) | 1982-06-01 |
Family
ID=5845810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK258373A DK144113C (en) | 1972-05-25 | 1973-05-10 | GROWTH PROMOTING FEED AND ADDITIVE TO USE IN FEED |
Country Status (7)
| Country | Link |
|---|---|
| BE (1) | BE799923A (en) |
| DE (1) | DE2225363C3 (en) |
| DK (1) | DK144113C (en) |
| FR (1) | FR2193550B1 (en) |
| GB (1) | GB1434582A (en) |
| IT (1) | IT1046494B (en) |
| NL (1) | NL180903C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2921213A1 (en) * | 1979-05-25 | 1980-12-11 | Henkel Kgaa | ANIMAL FEED MIX |
| GB8704058D0 (en) * | 1987-02-20 | 1987-03-25 | Porter W L | Probiotic-type products |
| WO2015175667A1 (en) * | 2014-05-13 | 2015-11-19 | Microbial Discovery Group, Llc | Direct-fed microbials and methods of their use |
| CA3016203A1 (en) | 2016-02-29 | 2017-09-08 | Microbial Discovery Group, Llc | Direct-fed microbials |
| BR112021005767A2 (en) | 2018-09-28 | 2021-06-22 | Microbial Discovery Group, Llc | microorganisms for plant pathogen inhibition |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3655396A (en) * | 1968-05-14 | 1972-04-11 | Japan Maize Prod | Process for preparing pulverized feed for animals |
| DE1940488A1 (en) * | 1968-09-13 | 1971-02-11 | Godo Shusei Kk | Process for the production of protease by culturing bacteria |
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1972
- 1972-05-25 DE DE2225363A patent/DE2225363C3/en not_active Expired
- 1972-10-16 FR FR7236577A patent/FR2193550B1/fr not_active Expired
-
1973
- 1973-05-10 DK DK258373A patent/DK144113C/en not_active IP Right Cessation
- 1973-05-10 NL NLAANVRAGE7306535,A patent/NL180903C/en not_active IP Right Cessation
- 1973-05-18 IT IT68455/73A patent/IT1046494B/en active
- 1973-05-23 BE BE131427A patent/BE799923A/en not_active IP Right Cessation
- 1973-05-24 GB GB2493773A patent/GB1434582A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DK144113C (en) | 1982-06-01 |
| NL7306535A (en) | 1973-11-27 |
| IT1046494B (en) | 1980-06-30 |
| NL180903B (en) | 1986-12-16 |
| GB1434582A (en) | 1976-05-05 |
| FR2193550A1 (en) | 1974-02-22 |
| DE2225363C3 (en) | 1981-01-22 |
| NL180903C (en) | 1987-05-18 |
| BE799923A (en) | 1973-11-23 |
| DE2225363B2 (en) | 1980-05-08 |
| DE2225363A1 (en) | 1973-12-20 |
| FR2193550B1 (en) | 1976-02-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PBP | Patent lapsed |