DK0894091T3 - Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning - Google Patents

Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning

Info

Publication number
DK0894091T3
DK0894091T3 DK97914167T DK97914167T DK0894091T3 DK 0894091 T3 DK0894091 T3 DK 0894091T3 DK 97914167 T DK97914167 T DK 97914167T DK 97914167 T DK97914167 T DK 97914167T DK 0894091 T3 DK0894091 T3 DK 0894091T3
Authority
DK
Denmark
Prior art keywords
purifying
protein
proteinaceous solution
cellulase
crystalline form
Prior art date
Application number
DK97914167T
Other languages
Danish (da)
English (en)
Inventor
Anders Rancke-Madsen
Mads Aage Laustsen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK0894091T3 publication Critical patent/DK0894091T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DK97914167T 1996-03-15 1997-03-14 Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning DK0894091T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK30596 1996-03-15
PCT/DK1997/000113 WO1997034919A1 (fr) 1996-03-15 1997-03-14 Procede de purification d'une proteine presente dans une solution contenant des proteines

Publications (1)

Publication Number Publication Date
DK0894091T3 true DK0894091T3 (da) 2003-06-10

Family

ID=8092063

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97914167T DK0894091T3 (da) 1996-03-15 1997-03-14 Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning

Country Status (10)

Country Link
US (1) US6087148A (fr)
EP (1) EP0894091B1 (fr)
JP (1) JP2000506534A (fr)
CN (1) CN1216899C (fr)
AT (1) ATE233784T1 (fr)
AU (1) AU2151997A (fr)
DE (1) DE69719497T2 (fr)
DK (1) DK0894091T3 (fr)
IN (1) IN190120B (fr)
WO (1) WO1997034919A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000506534A (ja) * 1996-03-15 2000-05-30 ノボ ノルディスク アクティーゼルスカブ タンパク質含有溶液からのタンパク質の精製方法
AU757991B2 (en) * 1998-04-27 2003-03-13 Altus Pharmaceuticals, Inc. Stabilized protein crystals, formulations containing them and methods of making them
DE19859876A1 (de) * 1998-12-23 2000-06-29 Fraunhofer Ges Forschung Verfahren zur Kristallisation aus Lösungen
GB9910975D0 (en) 1999-05-13 1999-07-14 Univ Strathclyde Rapid dehydration of proteins
US6663602B2 (en) 2000-06-16 2003-12-16 Novo Nordisk A/S Injection device
WO2003035674A1 (fr) * 2001-10-25 2003-05-01 Kyowa Hakko Kogyo Co., Ltd. Cristal de glutathione oxydee et son procede de production
EP1596667B1 (fr) * 2002-11-01 2009-01-21 Bayer HealthCare LLC Procédure pour la concentration de protéines
US7686786B2 (en) 2004-10-21 2010-03-30 Novo Nordiks A/S Dial-down mechanism for wind-up pen
EP2004671B1 (fr) 2006-03-30 2015-12-09 Novo Nordisk A/S Deux phases précipitation des protèines
US8226618B2 (en) 2006-05-16 2012-07-24 Novo Nordisk A/S Gearing mechanism for an injection device
RU2433840C2 (ru) 2006-05-18 2011-11-20 Ново Нордиск А/С Инъецирующее устройство со средством блокирования функции
DE102008029401A1 (de) * 2008-06-23 2009-12-24 Bayer Technology Services Gmbh Verfahren zur Kristallisation von Peptiden und Proteinen
FR2943686B1 (fr) * 2009-03-30 2013-11-01 Roquette Freres Procede d'obtention d'une preparation de beta-amylases a partir des fractions solubles de plantes amidonnieres
US9533106B2 (en) 2011-12-29 2017-01-03 Novo Nordisk A/S Torsion-spring based wind-up auto injector pen with dial-up/dial-down mechanism
WO2023200284A1 (fr) * 2022-04-15 2023-10-19 재단법인대구경북과학기술원 Procédé de purification et de concentration d'une protéine associée à des lipides dans un échantillon biologique pour la réalisation d'une spectrométrie de masse de la protéine associée à des lipides
WO2024032881A1 (fr) * 2022-08-10 2024-02-15 Arla Foods Amba Cristallisation d'une protéine pouvant cristalliser dans des conditions de salage à l'aide de multiples charges protéiques

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3664926A (en) * 1970-02-26 1972-05-23 Merck & Co Inc Crystallization of l-asparaginase
DE4001238A1 (de) * 1990-01-18 1991-07-25 Basf Ag Verfahren zur anreicherung bzw. reinigung von biomolekuelen
DK115890D0 (da) * 1990-05-09 1990-05-09 Novo Nordisk As Enzym
DK83093D0 (da) * 1993-07-09 1993-07-09 Novo Nordisk As Fremgangsmaade
JP2000506534A (ja) * 1996-03-15 2000-05-30 ノボ ノルディスク アクティーゼルスカブ タンパク質含有溶液からのタンパク質の精製方法

Also Published As

Publication number Publication date
DE69719497D1 (de) 2003-04-10
DE69719497T2 (de) 2003-10-30
CN1216899C (zh) 2005-08-31
ATE233784T1 (de) 2003-03-15
WO1997034919A1 (fr) 1997-09-25
EP0894091B1 (fr) 2003-03-05
IN190120B (fr) 2003-06-14
US6087148A (en) 2000-07-11
JP2000506534A (ja) 2000-05-30
AU2151997A (en) 1997-10-10
EP0894091A1 (fr) 1999-02-03
CN1213377A (zh) 1999-04-07

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