DK0611157T3 - Fremgangsmåde til fremstilling af nukleinsyrer fra en biologisk prøve ved anvendelse af lavt pH og sur protease - Google Patents
Fremgangsmåde til fremstilling af nukleinsyrer fra en biologisk prøve ved anvendelse af lavt pH og sur proteaseInfo
- Publication number
- DK0611157T3 DK0611157T3 DK94300971T DK94300971T DK0611157T3 DK 0611157 T3 DK0611157 T3 DK 0611157T3 DK 94300971 T DK94300971 T DK 94300971T DK 94300971 T DK94300971 T DK 94300971T DK 0611157 T3 DK0611157 T3 DK 0611157T3
- Authority
- DK
- Denmark
- Prior art keywords
- biological sample
- preparation
- low
- nucleic acids
- acid protease
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
- C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/015,729 US5386024A (en) | 1993-02-10 | 1993-02-10 | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0611157T3 true DK0611157T3 (da) | 2003-05-19 |
Family
ID=21773244
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK94300971T DK0611157T3 (da) | 1993-02-10 | 1994-02-10 | Fremgangsmåde til fremstilling af nukleinsyrer fra en biologisk prøve ved anvendelse af lavt pH og sur protease |
Country Status (11)
Country | Link |
---|---|
US (2) | US5386024A (fr) |
EP (1) | EP0611157B1 (fr) |
JP (1) | JP3477205B2 (fr) |
KR (1) | KR960701081A (fr) |
AT (1) | ATE238430T1 (fr) |
AU (2) | AU690895B2 (fr) |
CA (1) | CA2155744C (fr) |
DE (1) | DE69432540T2 (fr) |
DK (1) | DK0611157T3 (fr) |
ES (1) | ES2198411T3 (fr) |
WO (1) | WO1994018238A1 (fr) |
Families Citing this family (57)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5545539A (en) * | 1994-10-18 | 1996-08-13 | Genzyme Corporation | Method for nucleotide sequence amplification |
US6329139B1 (en) | 1995-04-25 | 2001-12-11 | Discovery Partners International | Automated sorting system for matrices with memory |
US6331273B1 (en) | 1995-04-25 | 2001-12-18 | Discovery Partners International | Remotely programmable matrices with memories |
US5751629A (en) | 1995-04-25 | 1998-05-12 | Irori | Remotely programmable matrices with memories |
US6017496A (en) | 1995-06-07 | 2000-01-25 | Irori | Matrices with memories and uses thereof |
US6416714B1 (en) * | 1995-04-25 | 2002-07-09 | Discovery Partners International, Inc. | Remotely programmable matrices with memories |
US5874214A (en) | 1995-04-25 | 1999-02-23 | Irori | Remotely programmable matrices with memories |
US5981235A (en) * | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
US6699986B2 (en) | 1998-08-04 | 2004-03-02 | Ortho-Clinical Diagnostics, Inc. | Electrophoretic separation of nucleic acids from proteins at low ph |
ATE454469T1 (de) | 2002-01-28 | 2010-01-15 | Ambion Inc | Für den nachweis von nukleinsäuren kompetente biologische rohderivate |
DE10237317B4 (de) * | 2002-08-15 | 2010-04-08 | 3M Espe Ag | Enzymhaltige Zusammensetzung, Verfahren zu deren Herstellung und deren Verwendung |
US7601491B2 (en) * | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
CN100395257C (zh) * | 2003-05-08 | 2008-06-18 | 慈溪市中鼎生物技术有限公司 | 钾离子酸性水溶液和利用这种溶液的dna提取方法和试剂盒 |
US20050164204A1 (en) * | 2004-01-27 | 2005-07-28 | Reed Thomas D. | Single use lyophilized rnase reagents, and kits and methods for using same |
EP2251441B1 (fr) * | 2004-02-10 | 2013-05-08 | F. Hoffmann-La Roche AG | Nouvelles amorces et sondes pour la détection du parvovirus B19 |
US7794932B2 (en) * | 2004-04-16 | 2010-09-14 | Piotr Chomczynski | Reagents and methods for isolation of purified RNA |
EP1600141B1 (fr) * | 2004-05-24 | 2013-04-17 | 3M Deutschland GmbH | Compositions contenant une enzyme active collagénolytique et son utilisation pour le traitement des carries dentaires |
US20050277121A1 (en) * | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
CA2839092C (fr) * | 2004-08-03 | 2018-04-03 | Becton, Dickinson And Company | Utilisation d'un materiau magnetique permettant de proceder a l'isoleme t de composes et le fractionnement d'echantillons constitues de plusieu s parties |
JP2008511816A (ja) * | 2004-08-03 | 2008-04-17 | ベクトン・ディキンソン・アンド・カンパニー | サンプルを分別するための磁性材料の使用 |
WO2006027780A2 (fr) * | 2004-09-08 | 2006-03-16 | Ramot At Tel Aviv University Ltd. | Nanostructures peptidiques contenant des peptides modifies par coiffage terminal, et procedes de generation et d'utilisation de celles-ci |
US20060188892A1 (en) * | 2005-02-18 | 2006-08-24 | Ambion, Inc. | Enzymatic digestion of tissue |
DE602006019203D1 (de) * | 2005-02-18 | 2011-02-10 | Gen Probe Inc | Probenherstellungsverfahren mit einem alkali-stoss |
EP1880206B1 (fr) * | 2005-05-09 | 2012-11-14 | Affymetrix, Inc. | Capture multiplex d'acides nucleiques |
US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
EP1880025B1 (fr) | 2005-05-12 | 2011-03-16 | Affymetrix, Inc. | Essais réalisés sur de l'adn à chaîne ramifiée multiplexe |
DK2500439T4 (da) * | 2005-06-20 | 2017-11-13 | Advanced Cell Diagnostics Inc | Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer |
US20070042408A1 (en) * | 2005-08-02 | 2007-02-22 | Michael Knoll | Nucleotide sequence for assessing transmissible spongiform encephalopathies |
EP1749892B1 (fr) | 2005-08-02 | 2008-03-19 | Roche Diagnostics GmbH | Séquence nucléotidique permettant d'évaluer TSE |
EP1754464A1 (fr) * | 2005-08-17 | 2007-02-21 | 3M Innovative Properties Company | Composition dentale contenant une enzyme |
US7569367B2 (en) | 2005-09-07 | 2009-08-04 | Roche Diagnostics Operations, Inc. | Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy |
US7927798B2 (en) * | 2005-10-05 | 2011-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
EP1932913B1 (fr) | 2006-12-11 | 2013-01-16 | Roche Diagnostics GmbH | Isolation d'acide nucléique utilisant du polidocanol et des dérivés |
JP5354894B2 (ja) | 2006-12-11 | 2013-11-27 | エフ.ホフマン−ラ ロシュ アーゲー | ポリドカノールおよび誘導体を用いた核酸単離 |
US8535888B2 (en) * | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
ES2665280T3 (es) * | 2007-06-29 | 2018-04-25 | Becton, Dickinson And Company | Métodos para la extracción y purificación de componentes de muestras biológicas |
EP2130929B1 (fr) | 2008-06-06 | 2013-10-09 | F. Hoffmann-La Roche AG | Détection multiplexe contrôlée en interne et quantification d'acides nucléiques microbiens |
ES2606688T3 (es) | 2010-07-29 | 2017-03-27 | F. Hoffmann-La Roche Ag | Ácidos nucleicos de control para parámetros múltiples |
EP2598655B1 (fr) | 2010-07-29 | 2016-10-05 | F.Hoffmann-La Roche Ag | Détection qualitative et quantitative d'acides nucléiques microbiens |
WO2012013733A1 (fr) | 2010-07-29 | 2012-02-02 | F. Hoffmann - La Roche Ag | Préparation d'échantillons génériques |
WO2012013732A1 (fr) | 2010-07-29 | 2012-02-02 | F. Hoffmann - La Roche Ag | Pcr générique |
EP2426222A1 (fr) | 2010-09-07 | 2012-03-07 | F. Hoffmann-La Roche AG | Tampon générique pour amplification |
AU2011301804B2 (en) | 2010-09-16 | 2015-07-16 | Gen-Probe Incorporated | Capture probes immobilizable via L-nucleotide tail |
EP3034625B1 (fr) | 2010-10-21 | 2017-10-04 | Advanced Cell Diagnostics, Inc. | Procédé ultrasensible de détection in situ d'acides nucléiques |
EP3388532B1 (fr) | 2010-11-01 | 2021-03-10 | Gen-Probe Incorporated | Capture intégrée et amplification d'acide nucléique cible de séquençage |
EP2465945A1 (fr) | 2010-12-17 | 2012-06-20 | F. Hoffmann-La Roche AG | Matrice générique pour acides nucléiques de contrôle |
WO2013067069A2 (fr) * | 2011-10-31 | 2013-05-10 | Leinweber Barbara | Procédé de digestion double pour récupération d'acide nucléique à grand rendement |
AU2013205603B2 (en) | 2012-02-01 | 2016-03-17 | Gen-Probe Incorporated | Asymmetric hairpin target capture oligomers |
US10017759B2 (en) * | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
CN107636164B (zh) | 2015-05-29 | 2022-02-18 | 豪夫迈·罗氏有限公司 | 用柠康酸酐灭活微生物的方法 |
WO2017066211A1 (fr) | 2015-10-12 | 2017-04-20 | Advanced Cell Diagnostics, Inc. | Détection in situ de variants nucléotidiques dans des échantillons de bruit élevé et compositions et procédés associés |
AU2017361521B2 (en) | 2016-11-21 | 2020-08-27 | Nanostring Technologies, Inc. | Chemical compositions and methods of using same |
US11549139B2 (en) | 2018-05-14 | 2023-01-10 | Nanostring Technologies, Inc. | Chemical compositions and methods of using same |
WO2022209943A1 (fr) | 2021-03-29 | 2022-10-06 | 東レ株式会社 | Procédé de détection de microarn dans un échantillon biologique, composition de chlorhydrate d'acide aminé neutre et récipient |
WO2023175434A1 (fr) | 2022-03-15 | 2023-09-21 | Diagenode S.A. | Détection de l'état de méthylation d'un échantillon d'adn |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
JPH04505556A (ja) | 1989-03-07 | 1992-10-01 | シンジェン,インコーポレイテッド | 細胞の検出または分離のための浮遊状態におけるインシトゥハイブリッド形成法 |
US5128247A (en) * | 1989-08-14 | 1992-07-07 | Board Of Regents, The University Of Texas System | Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources |
US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
-
1993
- 1993-02-10 US US08/015,729 patent/US5386024A/en not_active Expired - Lifetime
-
1994
- 1994-02-07 CA CA002155744A patent/CA2155744C/fr not_active Expired - Fee Related
- 1994-02-07 KR KR1019950703298A patent/KR960701081A/ko not_active Application Discontinuation
- 1994-02-07 JP JP51831894A patent/JP3477205B2/ja not_active Expired - Fee Related
- 1994-02-07 AU AU61359/94A patent/AU690895B2/en not_active Ceased
- 1994-02-07 WO PCT/US1994/001421 patent/WO1994018238A1/fr active Application Filing
- 1994-02-10 AT AT94300971T patent/ATE238430T1/de not_active IP Right Cessation
- 1994-02-10 ES ES94300971T patent/ES2198411T3/es not_active Expired - Lifetime
- 1994-02-10 DK DK94300971T patent/DK0611157T3/da active
- 1994-02-10 EP EP94300971A patent/EP0611157B1/fr not_active Expired - Lifetime
- 1994-02-10 DE DE69432540T patent/DE69432540T2/de not_active Expired - Fee Related
- 1994-11-30 US US08/347,272 patent/US6610475B1/en not_active Expired - Lifetime
-
1998
- 1998-06-04 AU AU69936/98A patent/AU6993698A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
ES2198411T3 (es) | 2004-02-01 |
JP3477205B2 (ja) | 2003-12-10 |
US6610475B1 (en) | 2003-08-26 |
WO1994018238A1 (fr) | 1994-08-18 |
JPH08508637A (ja) | 1996-09-17 |
DE69432540T2 (de) | 2003-11-06 |
DE69432540D1 (de) | 2003-05-28 |
KR960701081A (ko) | 1996-02-24 |
AU690895B2 (en) | 1998-05-07 |
EP0611157A2 (fr) | 1994-08-17 |
AU6993698A (en) | 1998-07-30 |
EP0611157B1 (fr) | 2003-04-23 |
CA2155744C (fr) | 2007-05-15 |
US5386024A (en) | 1995-01-31 |
EP0611157A3 (fr) | 1994-12-28 |
AU6135994A (en) | 1994-08-29 |
ATE238430T1 (de) | 2003-05-15 |
CA2155744A1 (fr) | 1994-08-18 |
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