DK0496327T3 - Polypetides with prourokinase activity, for these coding plasmids and methods for their preparation and their use - Google Patents

Polypetides with prourokinase activity, for these coding plasmids and methods for their preparation and their use

Info

Publication number
DK0496327T3
DK0496327T3 DK92100864T DK92100864T DK0496327T3 DK 0496327 T3 DK0496327 T3 DK 0496327T3 DK 92100864 T DK92100864 T DK 92100864T DK 92100864 T DK92100864 T DK 92100864T DK 0496327 T3 DK0496327 T3 DK 0496327T3
Authority
DK
Denmark
Prior art keywords
polypetides
preparation
methods
rscupa
amino acids
Prior art date
Application number
DK92100864T
Other languages
Danish (da)
Inventor
Leopold Prof Dr Flohe
Regina E Brigelius-Flohe
Wolfgang A Dr Guenzler
Bernhard Dr Wolf
Gerd Josef Steffens
Original Assignee
Leopold Prof Dr Flohe
Steffens Gerd Josef Prof Dr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leopold Prof Dr Flohe, Steffens Gerd Josef Prof Dr filed Critical Leopold Prof Dr Flohe
Application granted granted Critical
Publication of DK0496327T3 publication Critical patent/DK0496327T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3153—Streptokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Polypeptides of the general formula Ser-X1-X2-rscuPA<143-411> (1) in which X1 and X2 are defined amino acids or lower peptide bridges, X2 can also be a single bond, and rscuPA<143-411> is the unglycosylated sequence region of amino acids 143 (Glu) to 411 (LeuOH) from scu-PA 54 k, which can be used as plasminogen activators are described. To obtain these compounds, plasmids which are described in detail as such or in terms of their isolation were produced and expression thereof in suitable bacterial hosts provides, in each case in high yields, an expression product which does not act as plasminogen activator but whose protein-chemical conversion then leads to the single-chain compounds of the formula I which are valuable as plasminogen activators. <IMAGE>
DK92100864T 1991-01-22 1992-01-20 Polypetides with prourokinase activity, for these coding plasmids and methods for their preparation and their use DK0496327T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4101736A DE4101736A1 (en) 1991-01-22 1991-01-22 NEW PLASMINOGENACTIVATORS USEFUL POLYPEPTIDES, DAFUER CODING PLASMIDS AND METHOD FOR THE PRODUCTION THEREOF AND THEIR USE

Publications (1)

Publication Number Publication Date
DK0496327T3 true DK0496327T3 (en) 2001-12-03

Family

ID=6423453

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92100864T DK0496327T3 (en) 1991-01-22 1992-01-20 Polypetides with prourokinase activity, for these coding plasmids and methods for their preparation and their use

Country Status (15)

Country Link
EP (1) EP0496327B1 (en)
JP (1) JPH06169770A (en)
AT (1) ATE204326T1 (en)
DE (2) DE4101736A1 (en)
DK (1) DK0496327T3 (en)
ES (1) ES2164048T3 (en)
FI (1) FI920263A (en)
HK (1) HK1010108A1 (en)
HU (1) HU212510B (en)
IE (1) IE914040A1 (en)
IL (1) IL100163A0 (en)
LT (1) LT3948B (en)
LV (1) LV10974A (en)
PT (1) PT496327E (en)
RU (1) RU2108387C1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780303A (en) * 1990-04-06 1998-07-14 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US6017877A (en) * 1990-04-06 2000-01-25 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US5672585A (en) * 1990-04-06 1997-09-30 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US6521594B1 (en) 1990-04-06 2003-02-18 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
KR100225511B1 (en) * 1994-12-30 1999-10-15 성재갑 Process for the mass production of bovine growth hormone
JP2003521222A (en) * 1998-03-06 2003-07-15 アボット・ラボラトリーズ Highly crystalline urokinase

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI88932C (en) * 1982-04-15 1993-07-26 Genentech Inc Preparation of functional human urokinase protein
NL8601240A (en) 1986-05-15 1987-12-01 Leuven Res & Dev Vzw PLASMINOGEN ACTIVATOR AND THROMBOLYTIC MEDICINAL PRODUCT.
EP0316068A1 (en) * 1987-10-09 1989-05-17 Collaborative Research Inc. Modified low molecular weight plasminogen activator and method of preparation
DE3735917A1 (en) * 1987-10-23 1989-05-03 Basf Ag NEW POLYPEPTIDES, THEIR PRODUCTION AND USE
DE3810474A1 (en) * 1988-03-26 1989-10-05 Boehringer Ingelheim Int Expression vectors
GB8809093D0 (en) * 1988-04-18 1988-05-18 Erba Carlo Spa Low molecular weight derivatives of prourokinase
AU5515790A (en) * 1989-05-18 1990-11-22 Green Cross Corporation, The Human prourokinase variants, methods of producing same, dna sequences, plasmids and hosts therefor
IE901849A1 (en) * 1989-07-19 1991-06-19 Gruenenthal Gmbh Plasmids, their preparation and their use in the manufacture¹of a plasminogen activator

Also Published As

Publication number Publication date
DE59209915D1 (en) 2001-09-20
EP0496327B1 (en) 2001-08-16
RU2108387C1 (en) 1998-04-10
HK1010108A1 (en) 1999-06-11
LTIP1532A (en) 1995-06-26
FI920263A0 (en) 1992-01-21
DE4101736A1 (en) 1992-07-23
ATE204326T1 (en) 2001-09-15
HU9200127D0 (en) 1992-04-28
FI920263A (en) 1992-07-23
IE914040A1 (en) 1992-07-29
HU212510B (en) 1996-07-29
PT496327E (en) 2002-02-28
EP0496327A1 (en) 1992-07-29
JPH06169770A (en) 1994-06-21
LT3948B (en) 1996-05-27
HUT63877A (en) 1993-10-28
ES2164048T3 (en) 2002-02-16
LV10974A (en) 1995-12-20
IL100163A0 (en) 1992-08-18

Similar Documents

Publication Publication Date Title
CA2168429A1 (en) Expression of fusion polypeptides transported out of cytoplasm without leader sequences
DK601786A (en) HIS UNKNOWN PROCOAGULATION PROTEINS
MX9206041A (en) PROCESS TO PRODUCE HUMAN IMMUNE INTERFER.
ATE277078T1 (en) N-TERMINAL MODIFIED POLYPEPTIDES AND PRODUCTION PROCESS
HUT54213A (en) Process for the enzymatic removal of n-terminal sequence of proteins
ATE91722T1 (en) EQUINE GAMMA INTERFERON.
IL85020A (en) Pancreatic secretory trypsin inhibitor variants, their production, expression vectors and recombinant hosts therefor and pharmaceutical compositions containing them
NO910797D0 (en) DNA FRAGMENT CODING FOR A POLYPEPTIDE WITH NITRIL HYDRADASE ACTIVITY, A TRANSFORMANT CONTAINING GENET AND A PROCEDURE FOR THE PREPARATION OF AMIDES USING THE TRANSFORMER.
FR2638643B1 (en) DNA SEQUENCE ENCODING HUMAN FACTOR IX OR AN ANALOGUE PROTEIN, EXPRESSION VECTOR, TRANSFORMED CELLS, PROCESS FOR PREPARING FACTOR IX, AND PRODUCTS OBTAINED THEREFROM
DK164940C (en) POLYPEPTIDE WITH HUMAN-GAMMA INTERFERON ACTIVITY, GENETIC TECHNICAL PROCEDURE FOR PREPARING THEREOF, HYBRID PLASMIDS CONTAINING A DNA SEQUENCE CODING THE PEPTIDE, WATER ORGANIDE DETAILED SUMMARIA
HUT44614A (en) Process for preparing fusional proteins comprising eucaryotic ballast parts
DK0496327T3 (en) Polypetides with prourokinase activity, for these coding plasmids and methods for their preparation and their use
NO991995L (en) Nucleic acid and amino acid sequences related to Heliobacter pylori and vaccine compositions thereof
ES2114555T3 (en) NEW RECOMBINED HUMAN GAMMA INTERFERON.
EP0330700A4 (en) Human prourokinase-like polypeptide.
JPS6427473A (en) Human pancreas-secreting trypsin inhibitor and production thereof
NZ215994A (en) Culturing e.coli to produce interferon alpha and gamma and interleukin-2
FI895183A0 (en) FOERFARANDE FOER SELEKTIV SPJAELKNING AV FUSIONSPROTEINER.
EP0213085A3 (en) Vector for the expression and secretion of heterologous gene products in bacillus subtilis
TW243453B (en) Cloning and characterization of a cardiac adenylyl cyclase
MX9709846A (en) Nucleic acid and amino acid sequences relating to helicobacter pylori for diagnostics and therapeutics.