DK0486559T3 - Nukleinsyrefraktionering ved kapillarelektroforose med modvandring - Google Patents
Nukleinsyrefraktionering ved kapillarelektroforose med modvandringInfo
- Publication number
- DK0486559T3 DK0486559T3 DK90912127.9T DK90912127T DK0486559T3 DK 0486559 T3 DK0486559 T3 DK 0486559T3 DK 90912127 T DK90912127 T DK 90912127T DK 0486559 T3 DK0486559 T3 DK 0486559T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleic acid
- counter
- capillary electrophoresis
- fragments
- acid fractionation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44765—Apparatus specially adapted therefor of the counter-flow type
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/390,631 US5096554A (en) | 1989-08-07 | 1989-08-07 | Nucleic acid fractionation by counter-migration capillary electrophoresis |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0486559T3 true DK0486559T3 (da) | 1996-07-22 |
Family
ID=23543283
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK90912127.9T DK0486559T3 (da) | 1989-08-07 | 1990-08-06 | Nukleinsyrefraktionering ved kapillarelektroforose med modvandring |
Country Status (7)
Country | Link |
---|---|
US (2) | US5096554A (de) |
EP (1) | EP0486559B1 (de) |
JP (1) | JPH0797101B2 (de) |
AT (1) | ATE135606T1 (de) |
DE (1) | DE69026090T2 (de) |
DK (1) | DK0486559T3 (de) |
WO (1) | WO1991002244A1 (de) |
Families Citing this family (70)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5665216A (en) * | 1986-10-21 | 1997-09-09 | Northeastern University | Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same |
US5234824A (en) * | 1990-11-13 | 1993-08-10 | Specialty Laboratories, Inc. | Rapid purification of DNA |
US5126021A (en) * | 1991-07-17 | 1992-06-30 | Applied Biosystems Inc. | Low-viscosity polymer solution for capillary electrophoresis |
US5213669A (en) * | 1992-01-31 | 1993-05-25 | Beckman Instruments, Inc. | Capillary column containing a dynamically cross-linked composition and method of use |
GB2264496B (en) * | 1992-02-25 | 1995-10-25 | Us Energy | Sizing of fragments from a nucleic acid sequence |
EP0582256A3 (en) * | 1992-08-06 | 1997-09-17 | Hitachi Ltd | Polynucleotide detecting method and apparatus |
US5409586A (en) * | 1992-08-26 | 1995-04-25 | Hitachi, Ltd. | Method for analyzing nucleic acid or protein and apparatus therefor |
DE69316332D1 (de) * | 1992-08-26 | 1998-02-19 | Hitachi Ltd | Kapillar-Elektrophorese |
WO1994010564A1 (de) * | 1992-11-05 | 1994-05-11 | Evotec Biosystems Gmbh | Verfahren zur trennung von substanzen aus verdünnten lösungen oder suspensionen |
ES2055661B1 (es) * | 1993-01-20 | 1995-03-01 | Univ Malaga | Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre. |
WO1995008640A1 (en) * | 1993-09-23 | 1995-03-30 | E.I. Du Pont De Nemours And Company | An electrophoretic method for the isolation and separation of microorganisms |
US5391274A (en) * | 1993-10-18 | 1995-02-21 | Beckman Instruments, Inc. | Methods for controlling electroosmotic flow in coated capillary electrophoresis columns |
US5891313A (en) * | 1993-11-23 | 1999-04-06 | The Perkin-Elmer Corp. | Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks |
US5423966A (en) * | 1994-01-25 | 1995-06-13 | Perkin-Elmer Corporation | On line ion contaminant removal apparatus and method for capillary electrophoresis |
US6001229A (en) * | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
US5573651A (en) * | 1995-04-17 | 1996-11-12 | The Dow Chemical Company | Apparatus and method for flow injection analysis |
US6329139B1 (en) | 1995-04-25 | 2001-12-11 | Discovery Partners International | Automated sorting system for matrices with memory |
US6017496A (en) | 1995-06-07 | 2000-01-25 | Irori | Matrices with memories and uses thereof |
US5534123A (en) * | 1995-07-10 | 1996-07-09 | Molecular Dynamics | Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis |
US6221654B1 (en) * | 1996-09-25 | 2001-04-24 | California Institute Of Technology | Method and apparatus for analysis and sorting of polynucleotides based on size |
US6110343A (en) * | 1996-10-04 | 2000-08-29 | Lockheed Martin Energy Research Corporation | Material transport method and apparatus |
US5964995A (en) | 1997-04-04 | 1999-10-12 | Caliper Technologies Corp. | Methods and systems for enhanced fluid transport |
US5948227A (en) | 1997-12-17 | 1999-09-07 | Caliper Technologies Corp. | Methods and systems for performing electrophoretic molecular separations |
US6537432B1 (en) | 1998-02-24 | 2003-03-25 | Target Discovery, Inc. | Protein separation via multidimensional electrophoresis |
US6780591B2 (en) * | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
CA2332919A1 (en) | 1998-06-08 | 1999-12-16 | Caliper Technologies Corporation | Microfluidic devices, systems and methods for performing integrated reactions and separations |
AU758309B2 (en) * | 1999-02-02 | 2003-03-20 | Caliper Life Sciences, Inc. | Methods, devices and systems for characterizing proteins |
US6818112B2 (en) * | 1999-04-20 | 2004-11-16 | Target Discovery, Inc. | Protein separation via multidimensional electrophoresis |
US6764817B1 (en) * | 1999-04-20 | 2004-07-20 | Target Discovery, Inc. | Methods for conducting metabolic analyses |
US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
US7501245B2 (en) * | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
JP2004523243A (ja) | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
US6955670B2 (en) * | 2001-06-15 | 2005-10-18 | Martin Francis J | Nanopump system |
JP4657524B2 (ja) * | 2001-08-29 | 2011-03-23 | 嘉信 馬場 | マイクロチップ電気泳動用装置及びそれを用いる電気泳動方法 |
WO2003073233A2 (en) * | 2002-02-26 | 2003-09-04 | Pharmacia Corporation | Sequence detection system calculator |
US20030196896A1 (en) * | 2002-04-17 | 2003-10-23 | Mcwaid Thomas Harding | Method and apparatus for screening flowable separation media for electrophoresis and related applications |
US6887668B2 (en) * | 2002-04-19 | 2005-05-03 | Beckman Coulter, Inc. | Nucleic acid separation and detection by electrophoresis with a counter-migrating high-affinity intercalating dye |
US20050170367A1 (en) * | 2003-06-10 | 2005-08-04 | Quake Stephen R. | Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids |
US20050034990A1 (en) * | 2003-08-12 | 2005-02-17 | Crooks Richard M. | System and method for electrokinetic trapping and concentration enrichment of analytes in a microfluidic channel |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
WO2005054441A2 (en) * | 2003-12-01 | 2005-06-16 | California Institute Of Technology | Device for immobilizing chemical and biomedical species and methods of using same |
ATE463584T1 (de) | 2004-02-19 | 2010-04-15 | Helicos Biosciences Corp | Verfahren zur analyse von polynukleotidsequenzen |
US20060046258A1 (en) * | 2004-02-27 | 2006-03-02 | Lapidus Stanley N | Applications of single molecule sequencing |
US20050239085A1 (en) * | 2004-04-23 | 2005-10-27 | Buzby Philip R | Methods for nucleic acid sequence determination |
US20050260609A1 (en) * | 2004-05-24 | 2005-11-24 | Lapidus Stanley N | Methods and devices for sequencing nucleic acids |
US20070117104A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
US7635562B2 (en) * | 2004-05-25 | 2009-12-22 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
US20070117103A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
US7476734B2 (en) * | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
US20060024678A1 (en) * | 2004-07-28 | 2006-02-02 | Helicos Biosciences Corporation | Use of single-stranded nucleic acid binding proteins in sequencing |
US20060118754A1 (en) * | 2004-12-08 | 2006-06-08 | Lapen Daniel C | Stabilizing a polyelectrolyte multilayer |
US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
US20060172328A1 (en) * | 2005-01-05 | 2006-08-03 | Buzby Philip R | Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction |
US7482120B2 (en) * | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
US20060263790A1 (en) * | 2005-05-20 | 2006-11-23 | Timothy Harris | Methods for improving fidelity in a nucleic acid synthesis reaction |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
US20070117102A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
US20070128610A1 (en) * | 2005-12-02 | 2007-06-07 | Buzby Philip R | Sample preparation method and apparatus for nucleic acid sequencing |
WO2007070642A2 (en) * | 2005-12-15 | 2007-06-21 | Helicos Biosciences Corporation | Methods for increasing accuracy of nucleic acid sequencing |
US20080309926A1 (en) * | 2006-03-08 | 2008-12-18 | Aaron Weber | Systems and methods for reducing detected intensity non uniformity in a laser beam |
US7397546B2 (en) * | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
DE102009009650B4 (de) | 2009-02-19 | 2013-10-10 | Atotech Deutschland Gmbh | Verfahren und Vorrichtung zum Herstellen einer Kunststoffschicht sowie deren Verwendung |
DE112010002222B4 (de) * | 2009-06-04 | 2024-01-25 | Leidos Innovations Technology, Inc. (n.d.Ges.d. Staates Delaware) | Mehr-Proben-Mikrofluidchip fur DNA-Analyse |
WO2012051529A1 (en) | 2010-10-15 | 2012-04-19 | Lockheed Martin Corporation | Micro fluidic optic design |
US9322054B2 (en) | 2012-02-22 | 2016-04-26 | Lockheed Martin Corporation | Microfluidic cartridge |
JP6102711B2 (ja) * | 2013-12-10 | 2017-03-29 | 株式会社島津製作所 | 電気泳動分離方法 |
US11946900B2 (en) * | 2018-04-09 | 2024-04-02 | Shimadzu Corporation | Electrophoresis flow channel cleaning method and electrophoresis device |
DE102018009281A1 (de) * | 2018-11-24 | 2020-05-28 | Selectrion GmbH | Verfahren und Vorrichtung zur Separation von Ionen gleicher Ladungspolarität im elektrischen Feld |
WO2020124213A1 (en) * | 2018-12-20 | 2020-06-25 | Krylov Sergey N | Binder selection using capillary electrophoresis |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE390766B (sv) * | 1972-12-19 | 1977-01-17 | Lkb Produkter Ab | Forfarande vid motflodesisotachofores |
JPS60138448A (ja) * | 1983-12-27 | 1985-07-23 | Shimadzu Corp | 等速電気泳動分析方法 |
JPS60259945A (ja) * | 1984-06-05 | 1985-12-23 | Shimadzu Corp | 細管式等速電気泳動分析用リ−デイング液 |
US4865707A (en) * | 1986-10-21 | 1989-09-12 | Northeastern University | Capillary gel electrophoresis columns |
JPS63208763A (ja) * | 1987-02-25 | 1988-08-30 | Shimadzu Corp | 塩基配列決定装置 |
-
1989
- 1989-08-07 US US07/390,631 patent/US5096554A/en not_active Expired - Lifetime
-
1990
- 1990-08-06 DK DK90912127.9T patent/DK0486559T3/da active
- 1990-08-06 US US07/562,790 patent/US5110424A/en not_active Expired - Lifetime
- 1990-08-06 AT AT90912127T patent/ATE135606T1/de not_active IP Right Cessation
- 1990-08-06 JP JP2511646A patent/JPH0797101B2/ja not_active Expired - Fee Related
- 1990-08-06 EP EP90912127A patent/EP0486559B1/de not_active Expired - Lifetime
- 1990-08-06 DE DE69026090T patent/DE69026090T2/de not_active Expired - Fee Related
- 1990-08-06 WO PCT/US1990/004380 patent/WO1991002244A1/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
JPH0797101B2 (ja) | 1995-10-18 |
WO1991002244A1 (en) | 1991-02-21 |
EP0486559A1 (de) | 1992-05-27 |
JPH04507001A (ja) | 1992-12-03 |
EP0486559B1 (de) | 1996-03-20 |
DE69026090D1 (de) | 1996-04-25 |
ATE135606T1 (de) | 1996-04-15 |
US5110424A (en) | 1992-05-05 |
EP0486559A4 (en) | 1993-03-17 |
DE69026090T2 (de) | 1996-11-14 |
US5096554A (en) | 1992-03-17 |
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