DK0486559T3 - Nukleinsyrefraktionering ved kapillarelektroforose med modvandring - Google Patents

Nukleinsyrefraktionering ved kapillarelektroforose med modvandring

Info

Publication number
DK0486559T3
DK0486559T3 DK90912127.9T DK90912127T DK0486559T3 DK 0486559 T3 DK0486559 T3 DK 0486559T3 DK 90912127 T DK90912127 T DK 90912127T DK 0486559 T3 DK0486559 T3 DK 0486559T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
counter
capillary electrophoresis
fragments
acid fractionation
Prior art date
Application number
DK90912127.9T
Other languages
English (en)
Inventor
Allan Michael Chin
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of DK0486559T3 publication Critical patent/DK0486559T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44765—Apparatus specially adapted therefor of the counter-flow type

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK90912127.9T 1989-08-07 1990-08-06 Nukleinsyrefraktionering ved kapillarelektroforose med modvandring DK0486559T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/390,631 US5096554A (en) 1989-08-07 1989-08-07 Nucleic acid fractionation by counter-migration capillary electrophoresis

Publications (1)

Publication Number Publication Date
DK0486559T3 true DK0486559T3 (da) 1996-07-22

Family

ID=23543283

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90912127.9T DK0486559T3 (da) 1989-08-07 1990-08-06 Nukleinsyrefraktionering ved kapillarelektroforose med modvandring

Country Status (7)

Country Link
US (2) US5096554A (da)
EP (1) EP0486559B1 (da)
JP (1) JPH0797101B2 (da)
AT (1) ATE135606T1 (da)
DE (1) DE69026090T2 (da)
DK (1) DK0486559T3 (da)
WO (1) WO1991002244A1 (da)

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US5409586A (en) * 1992-08-26 1995-04-25 Hitachi, Ltd. Method for analyzing nucleic acid or protein and apparatus therefor
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EP0720658A1 (en) * 1993-09-23 1996-07-10 E.I. Du Pont De Nemours And Company An electrophoretic method for the isolation and separation of microorganisms
US5391274A (en) * 1993-10-18 1995-02-21 Beckman Instruments, Inc. Methods for controlling electroosmotic flow in coated capillary electrophoresis columns
US5891313A (en) * 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
US6001229A (en) * 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5573651A (en) * 1995-04-17 1996-11-12 The Dow Chemical Company Apparatus and method for flow injection analysis
US6017496A (en) 1995-06-07 2000-01-25 Irori Matrices with memories and uses thereof
US6329139B1 (en) 1995-04-25 2001-12-11 Discovery Partners International Automated sorting system for matrices with memory
US5534123A (en) * 1995-07-10 1996-07-09 Molecular Dynamics Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6110343A (en) * 1996-10-04 2000-08-29 Lockheed Martin Energy Research Corporation Material transport method and apparatus
US5964995A (en) * 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US5948227A (en) * 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
US6537432B1 (en) 1998-02-24 2003-03-25 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) * 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
WO1999064836A1 (en) 1998-06-08 1999-12-16 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
JP4515640B2 (ja) * 1999-02-02 2010-08-04 カリパー・ライフ・サイエンシズ・インク． タンパク質特性決定方法、装置及びシステム
US6818112B2 (en) 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6764817B1 (en) 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US6955670B2 (en) * 2001-06-15 2005-10-18 Martin Francis J Nanopump system
JP4657524B2 (ja) * 2001-08-29 2011-03-23 嘉信 馬場 マイクロチップ電気泳動用装置及びそれを用いる電気泳動方法
AU2003217749A1 (en) * 2002-02-26 2003-09-09 Pharmacia Corporation Sequence detection system calculator
US20030196896A1 (en) * 2002-04-17 2003-10-23 Mcwaid Thomas Harding Method and apparatus for screening flowable separation media for electrophoresis and related applications
US6887668B2 (en) * 2002-04-19 2005-05-03 Beckman Coulter, Inc. Nucleic acid separation and detection by electrophoresis with a counter-migrating high-affinity intercalating dye
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Also Published As

Publication number Publication date
EP0486559A1 (en) 1992-05-27
EP0486559A4 (en) 1993-03-17
JPH0797101B2 (ja) 1995-10-18
US5096554A (en) 1992-03-17
ATE135606T1 (de) 1996-04-15
US5110424A (en) 1992-05-05
DE69026090T2 (de) 1996-11-14
EP0486559B1 (en) 1996-03-20
DE69026090D1 (de) 1996-04-25
JPH04507001A (ja) 1992-12-03
WO1991002244A1 (en) 1991-02-21

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