DE69838754D1 - Verfahren zur herstellung von sialyloligosacchariden aus milch - Google Patents

Verfahren zur herstellung von sialyloligosacchariden aus milch

Info

Publication number
DE69838754D1
DE69838754D1 DE69838754T DE69838754T DE69838754D1 DE 69838754 D1 DE69838754 D1 DE 69838754D1 DE 69838754 T DE69838754 T DE 69838754T DE 69838754 T DE69838754 T DE 69838754T DE 69838754 D1 DE69838754 D1 DE 69838754D1
Authority
DE
Germany
Prior art keywords
sialyloligosaccharides
milk
preparing
preparing sialyloligosaccharides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69838754T
Other languages
English (en)
Other versions
DE69838754T2 (de
Inventor
Marc Pelletier
William A Barker
David J Hakes
David A Zopf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Neose Technologies Inc
Original Assignee
Neose Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neose Technologies Inc filed Critical Neose Technologies Inc
Publication of DE69838754D1 publication Critical patent/DE69838754D1/de
Application granted granted Critical
Publication of DE69838754T2 publication Critical patent/DE69838754T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/102—Caprine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/103—Ovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DE69838754T 1997-08-14 1998-08-13 Verfahren zur herstellung von sialyloligosacchariden aus milch Expired - Fee Related DE69838754T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US911393 1997-08-14
US08/911,393 US6323008B1 (en) 1997-08-14 1997-08-14 Methods for producing sialyloligosaccharides in a dairy source
PCT/US1998/016756 WO1999008511A1 (en) 1997-08-14 1998-08-13 Methods for producing sialyloligosaccharides in a dairy source

Publications (2)

Publication Number Publication Date
DE69838754D1 true DE69838754D1 (de) 2008-01-03
DE69838754T2 DE69838754T2 (de) 2008-10-30

Family

ID=25430171

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69838754T Expired - Fee Related DE69838754T2 (de) 1997-08-14 1998-08-13 Verfahren zur herstellung von sialyloligosacchariden aus milch

Country Status (7)

Country Link
US (3) US6323008B1 (de)
EP (1) EP1003366B1 (de)
JP (1) JP2001514865A (de)
AU (1) AU735910B2 (de)
CA (1) CA2300308A1 (de)
DE (1) DE69838754T2 (de)
WO (1) WO1999008511A1 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6323008B1 (en) * 1997-08-14 2001-11-27 Neose Technologies, Inc. Methods for producing sialyloligosaccharides in a dairy source
NZ507335A (en) * 2000-10-05 2004-10-29 New Zealand Dairy Board Bone health compositions derived from milk comprising an acidic protein fraction but that does not contain CGMP
NZ528190A (en) 2001-03-21 2005-06-24 Dsm Ip Assets B Cheese making process
US6875459B2 (en) * 2001-09-10 2005-04-05 Henry B. Kopf Method and apparatus for separation of milk, colostrum, and whey
AU2002356472A1 (en) * 2001-12-13 2003-06-23 New Zealand Dairy Board Dairy process and product
CN103463615B (zh) * 2005-02-24 2016-09-07 帝斯曼知识产权资产管理有限公司 来自糖巨肽的血压降低肽
AR056761A1 (es) * 2005-11-04 2007-10-24 Arla Foods Amba UN CONCENTRADO DERIVADO DE UN PRODUCTO LÁCTEO ENRIQUECIDO CON SALILLACTOSA NATURAL Y UN PROCEDIMIENTO PARA LA PREPARACIoN DEL MISMO
BRPI0709560A2 (pt) * 2006-03-17 2011-07-19 Vib Vzw uso de uma proteìna mutante de trans-sialidade de trypanosoma enzimaticamente inativa, e composição farmacêutica
WO2007114683A1 (en) * 2006-03-30 2007-10-11 N.V. Nutricia Milk oligosaccharides for stimulating the immune system
FR2907687B1 (fr) * 2006-10-30 2008-12-26 Applexion Procede de purification de sialyllactose par chromatographie
NZ581390A (en) * 2007-06-25 2012-09-28 Dsm Ip Assets Bv Prebiotics comprising an oligosaccharide, sialyloligosaccharide and free sialic acid
NL2001377C2 (nl) * 2008-03-14 2009-09-15 Friesland Brands Bv Werkwijze voor het isoleren van siaalzuur bevattende oligosachariden, alsmede de hiermee verkrijgbare siaalzuur bevattende oligosachariden bevatttende samenstellingen.
JP4682377B2 (ja) * 2009-10-06 2011-05-11 独立行政法人農業・食品産業技術総合研究機構 植物からのシアル酸含有化合物の抽出法
KR101116864B1 (ko) 2008-08-01 2012-02-29 주식회사 베네비오 고지혈증, 지방간 또는 비만의 예방 또는 치료용 조성물
EP2403862A1 (de) * 2009-03-04 2012-01-11 Nestec S.A. Oligosaccharidbestandteil
GB0904562D0 (en) * 2009-03-17 2009-04-29 Separation Technologies Invest Isolation and purification of components of whey
GB2468670A (en) * 2009-03-17 2010-09-22 Separation Technologies Invest Processing whey or raw milk
IT1394503B1 (it) † 2009-04-06 2012-07-05 Inalco Spa Sali di 6'-sialillattosio e processo per la loro sintesi e per la sintesi di altri alfa-sialiloligosaccaridi.
JP5599994B2 (ja) * 2009-09-29 2014-10-01 雪印メグミルク株式会社 シアリルラクトース素材の分離方法
JP5641598B2 (ja) * 2009-09-29 2014-12-17 雪印メグミルク株式会社 シアリルラクトース素材の分離方法
TR201802987T4 (tr) * 2010-02-03 2018-03-21 Dsm Ip Assets Bv Süt teknolojisinde siyalidaz kullanımı.
US20130251740A1 (en) * 2010-10-26 2013-09-26 Rockefeller University (The) Immunogenic agents
MX2013013028A (es) * 2011-05-13 2013-12-02 Glycom As Metodo para generar oligosacaridos de leche humana (hmo) o precursores de los mismos.
ES2820429T3 (es) * 2011-05-13 2021-04-21 Glycom As Diversificación de los oligosacáridos de la leche humana (HMO) o sus precursores
EP2984104B1 (de) * 2013-04-12 2017-11-01 Danmarks Tekniske Universitet Mutierte sialidase mit trans-sialidase aktivität zur herstellung von sialyrierten gylcanen
PL2896628T3 (pl) * 2014-01-20 2019-03-29 Jennewein Biotechnologie Gmbh Sposób wydajnego oczyszczania obojętnych oligosacharydów ludzkiego mleka (HMO) z fermentacji mikrobiologicznej
JP5812455B2 (ja) * 2014-07-15 2015-11-11 雪印メグミルク株式会社 シアリルラクトース素材の分離方法
JP6762027B2 (ja) * 2016-08-25 2020-09-30 株式会社雅嘉貿易 シアロオリゴ糖の製造方法とその利用
EP3450443A1 (de) * 2017-08-29 2019-03-06 Jennewein Biotechnologie GmbH Verfahren zur reinigung sialylierter oligosaccharide
GB201909888D0 (en) 2019-07-10 2019-08-21 Dairy Crest Ltd Methods for manufacturing comestible products
KR20220072866A (ko) * 2019-10-01 2022-06-02 글리콤 에이/에스 발효액으로부터 중성 올리고사카라이드의 분리
WO2022089707A1 (en) 2020-10-30 2022-05-05 Mille International Aps A method of producing a human milk oligosaccharide (hmo)

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US4001198A (en) 1974-05-28 1977-01-04 Thomas Frank A Method of recovering nutrients from cheese whey and purifying the effluent
US4202909A (en) 1976-11-22 1980-05-13 Patent Technology, Inc. Treatment of whey
US4617861A (en) 1982-10-07 1986-10-21 Fermentation Engineering, Inc. Whey treatment apparatus
JPS59184197A (ja) 1983-04-01 1984-10-19 Snow Brand Milk Prod Co Ltd シアル酸結合オリゴ糖の調製法
US4547386A (en) 1984-02-27 1985-10-15 Purdue Research Foundation Lactose/cheese whey/whey filtrate semi-solid animal feed supplement
US4543261A (en) 1984-03-02 1985-09-24 The Dow Chemical Company Separating whey components into high purity products by ion exchange
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US5164374A (en) 1990-12-17 1992-11-17 Monsanto Company Use of oligosaccharides for treatment of arthritis
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JPH0686684A (ja) * 1992-05-26 1994-03-29 Monsanto Co シアロ抱合体の合成
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US5728554A (en) 1995-04-11 1998-03-17 Cytel Corporation Enzymatic synthesis of glycosidic linkages
AU5445396A (en) 1995-04-11 1996-10-30 Cytel Corporation Improved enzymatic synthesis of oligosaccharides
US6323008B1 (en) * 1997-08-14 2001-11-27 Neose Technologies, Inc. Methods for producing sialyloligosaccharides in a dairy source

Also Published As

Publication number Publication date
AU8781598A (en) 1999-03-08
US6323008B1 (en) 2001-11-27
EP1003366A4 (de) 2004-11-10
US6706497B2 (en) 2004-03-16
AU735910B2 (en) 2001-07-19
DE69838754T2 (de) 2008-10-30
JP2001514865A (ja) 2001-09-18
US20020150995A1 (en) 2002-10-17
WO1999008511A1 (en) 1999-02-25
CA2300308A1 (en) 1999-02-25
US20040185146A1 (en) 2004-09-23
EP1003366B1 (de) 2007-11-21
EP1003366A1 (de) 2000-05-31

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