DE69813185D1 - Klonierung und Expression eines neuen Glukoamylasegens aus endomyces fibuliger - Google Patents

Klonierung und Expression eines neuen Glukoamylasegens aus endomyces fibuliger

Info

Publication number
DE69813185D1
DE69813185D1 DE69813185T DE69813185T DE69813185D1 DE 69813185 D1 DE69813185 D1 DE 69813185D1 DE 69813185 T DE69813185 T DE 69813185T DE 69813185 T DE69813185 T DE 69813185T DE 69813185 D1 DE69813185 D1 DE 69813185D1
Authority
DE
Germany
Prior art keywords
amino acid
enzyme
recombinant
cells
endomyces fibuliger
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69813185T
Other languages
English (en)
Other versions
DE69813185T2 (de
Inventor
Raymond David Pridmore
Sunil Kochhar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe des Produits Nestle SA
Nestle SA
Original Assignee
Societe des Produits Nestle SA
Nestle SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Societe des Produits Nestle SA, Nestle SA filed Critical Societe des Produits Nestle SA
Application granted granted Critical
Publication of DE69813185D1 publication Critical patent/DE69813185D1/de
Publication of DE69813185T2 publication Critical patent/DE69813185T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69813185T 1998-05-06 1998-05-06 Klonierung und Expression eines neuen Glukoamylasegens aus endomyces fibuliger Expired - Lifetime DE69813185T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98201473A EP0959130B9 (de) 1998-05-06 1998-05-06 Klonierung und Expression eines neuen Glukoamylasegens aus endomyces fibuliger

Publications (2)

Publication Number Publication Date
DE69813185D1 true DE69813185D1 (de) 2003-05-15
DE69813185T2 DE69813185T2 (de) 2004-03-04

Family

ID=8233687

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69813185T Expired - Lifetime DE69813185T2 (de) 1998-05-06 1998-05-06 Klonierung und Expression eines neuen Glukoamylasegens aus endomyces fibuliger

Country Status (4)

Country Link
EP (1) EP0959130B9 (de)
AT (1) ATE236982T1 (de)
DE (1) DE69813185T2 (de)
DK (1) DK0959130T3 (de)

Also Published As

Publication number Publication date
DE69813185T2 (de) 2004-03-04
DK0959130T3 (da) 2003-08-04
EP0959130B1 (de) 2003-04-09
EP0959130A1 (de) 1999-11-24
ATE236982T1 (de) 2003-04-15
EP0959130B9 (de) 2003-10-29

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