DE69737475D1 - Mit makro- und mikroabtastobjektiven kompatibles fluoreszenzabbildungssystem - Google Patents

Mit makro- und mikroabtastobjektiven kompatibles fluoreszenzabbildungssystem

Info

Publication number
DE69737475D1
DE69737475D1 DE69737475T DE69737475T DE69737475D1 DE 69737475 D1 DE69737475 D1 DE 69737475D1 DE 69737475 T DE69737475 T DE 69737475T DE 69737475 T DE69737475 T DE 69737475T DE 69737475 D1 DE69737475 D1 DE 69737475D1
Authority
DE
Germany
Prior art keywords
microbutastobjectives
macro
imaging system
fluorescence imaging
compatible fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69737475T
Other languages
English (en)
Other versions
DE69737475T2 (de
Inventor
Robert C Kain
Christopher C Alexay
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Integenx Acquisition Corp
Original Assignee
GE Healthcare SV Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/595,355 external-priority patent/US5646411A/en
Application filed by GE Healthcare SV Corp filed Critical GE Healthcare SV Corp
Publication of DE69737475D1 publication Critical patent/DE69737475D1/de
Application granted granted Critical
Publication of DE69737475T2 publication Critical patent/DE69737475T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
DE69737475T 1996-02-01 1997-01-31 Mit makro- und mikroabtastobjektiven kompatibles fluoreszenzabbildungssystem Expired - Lifetime DE69737475T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/595,355 US5646411A (en) 1996-02-01 1996-02-01 Fluorescence imaging system compatible with macro and micro scanning objectives
US595355 1996-02-01
US08/791,684 US5847400A (en) 1996-02-01 1997-01-30 Fluorescence imaging system having reduced background fluorescence
US791684 1997-01-30
PCT/US1997/001461 WO1997028439A1 (en) 1996-02-01 1997-01-31 Fluorescence imaging system compatible with macro and micro scanning objectives

Publications (2)

Publication Number Publication Date
DE69737475D1 true DE69737475D1 (de) 2007-04-26
DE69737475T2 DE69737475T2 (de) 2007-07-12

Family

ID=27082245

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69737475T Expired - Lifetime DE69737475T2 (de) 1996-02-01 1997-01-31 Mit makro- und mikroabtastobjektiven kompatibles fluoreszenzabbildungssystem

Country Status (5)

Country Link
US (1) US5847400A (de)
EP (2) EP0880690B1 (de)
JP (1) JP4405591B2 (de)
DE (1) DE69737475T2 (de)
WO (1) WO1997028439A1 (de)

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CN103543094B (zh) * 2007-12-19 2017-06-09 神谷来克斯公司 单分子检测用扫描分析器和使用方法
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Also Published As

Publication number Publication date
WO1997028439A1 (en) 1997-08-07
EP1783528A2 (de) 2007-05-09
DE69737475T2 (de) 2007-07-12
JP2002505011A (ja) 2002-02-12
EP1783528A3 (de) 2008-07-16
EP0880690B1 (de) 2007-03-14
JP4405591B2 (ja) 2010-01-27
EP0880690A1 (de) 1998-12-02
EP0880690A4 (de) 2000-07-19
US5847400A (en) 1998-12-08

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