DE69717001T2 - Verfahren zur Bestimmung des Endpunktes von enzymatischen Nachweisverfahren - Google Patents

Verfahren zur Bestimmung des Endpunktes von enzymatischen Nachweisverfahren

Info

Publication number
DE69717001T2
DE69717001T2 DE69717001T DE69717001T DE69717001T2 DE 69717001 T2 DE69717001 T2 DE 69717001T2 DE 69717001 T DE69717001 T DE 69717001T DE 69717001 T DE69717001 T DE 69717001T DE 69717001 T2 DE69717001 T2 DE 69717001T2
Authority
DE
Germany
Prior art keywords
determining
end point
detection methods
enzymatic detection
enzymatic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69717001T
Other languages
English (en)
Other versions
DE69717001D1 (de
Inventor
Michael J Pugia
Robert J Schaeper
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Inc
Original Assignee
Bayer AG
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG
Application granted granted Critical
Publication of DE69717001D1 publication Critical patent/DE69717001D1/de
Publication of DE69717001T2 publication Critical patent/DE69717001T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/70—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases the product, e.g. phenol, naphthol being diazotised in situ, e.g. with Fast Red
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/966—Elastase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69717001T 1996-05-28 1997-05-16 Verfahren zur Bestimmung des Endpunktes von enzymatischen Nachweisverfahren Expired - Lifetime DE69717001T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/653,865 US5846754A (en) 1996-05-28 1996-05-28 Enzyme determination with protease inactivation

Publications (2)

Publication Number Publication Date
DE69717001D1 DE69717001D1 (de) 2002-12-19
DE69717001T2 true DE69717001T2 (de) 2003-07-17

Family

ID=24622589

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69717001T Expired - Lifetime DE69717001T2 (de) 1996-05-28 1997-05-16 Verfahren zur Bestimmung des Endpunktes von enzymatischen Nachweisverfahren

Country Status (5)

Country Link
US (1) US5846754A (de)
EP (1) EP0810290B1 (de)
JP (1) JPH1042895A (de)
CA (1) CA2198951C (de)
DE (1) DE69717001T2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19814761A1 (de) * 1998-04-02 1999-10-07 Merck Patent Gmbh Verfahren und Mittel zur Bestimmung von Proteinasen
US6348324B1 (en) 1999-01-21 2002-02-19 Hypoguard America Limited Composition and device for detecting leukocytes in urine
US6528652B1 (en) * 1999-01-21 2003-03-04 Chronimed Composition and device for detecting leukocytes in urine
FR2809820B1 (fr) * 2000-05-30 2002-10-25 Proteus Methode de detection d'une transformation d'un substrat et ses applications
FR2831889B1 (fr) * 2001-11-08 2004-01-16 Proteus Procede pour generer l'empreinte catalytique idiosyncrasique d'un echantillon, le traitement de ladite empreinte et les systemes pour leur mise en oeuvre
FR2831888A1 (fr) * 2001-11-08 2003-05-09 Proteus Procede pour generer l'empreinte catalytique idiosyncrasique d'un echantillon et les systemes pour sa mise en oeuvre
US7045297B2 (en) * 2001-11-14 2006-05-16 Prion Developmental Laboratories, Inc. Rapid prion-detection assay
FR2832729B1 (fr) * 2001-11-28 2004-01-16 Proteus Methode de detection d'une activite catalytique d'un echantillon mettant en oeuvre la detection de la transformation d'un substrat
AU2007207242B2 (en) 2006-01-19 2012-08-02 Lattec I/S Dry stick device and method for determining an analyte in a sample
PL1982182T3 (pl) 2006-01-19 2012-09-28 Lattec I/S Nowa konstrukcja przyrządu w postaci suchego paska oraz sposób oznaczania analitu w próbce przy użyciu przyrządu w postaci suchego paska
PL228200B1 (pl) 2008-11-18 2018-02-28 Adam Saferna Pojemnik wysokocisnieniowy
CN103946711B (zh) * 2011-11-11 2018-12-04 安讯希特技术有限公司 血样本的化验方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58187847A (ja) * 1982-04-28 1983-11-02 Toshiba Corp 電極装置の洗浄方法
DE3311887A1 (de) * 1983-03-31 1984-12-20 Byk-Mallinckrodt Chemische Produkte Gmbh, 6057 Dietzenbach Verfahren zur bestimmung der peroxidase-aktivitaet durch endverduennungstitration und mittel zur durchfuehrung des verfahrens
US4657855A (en) * 1984-04-06 1987-04-14 Miles Laboratories, Inc. Composition and test device for determining the presence of leukocytes, esterase and protease in a test sample
US4637979A (en) * 1984-04-06 1987-01-20 Miles Laboratories, Inc. Composition and test device for determining the presence of leukocytes containing a zwitterion coupling agent
DE3924442A1 (de) * 1988-07-26 1990-02-01 Konishiroku Photo Ind Mehrlagiges analysenelement zum testen der amylaseaktivitaet
JP2994831B2 (ja) * 1991-12-27 1999-12-27 国際試薬株式会社 コレステロールの定量法および定量用試薬
US5464739A (en) * 1994-08-22 1995-11-07 Bayer Corporation Composition method for determining the presence of leukocyte cells, esterase or protease in a test sample
CA2161574A1 (en) * 1994-11-15 1996-05-16 James Noffsinger Methodology for colorimetrically determining the concentration of white blood cells in a biological fluid

Also Published As

Publication number Publication date
EP0810290B1 (de) 2002-11-13
DE69717001D1 (de) 2002-12-19
US5846754A (en) 1998-12-08
EP0810290A2 (de) 1997-12-03
EP0810290A3 (de) 1998-06-17
CA2198951C (en) 2003-07-22
JPH1042895A (ja) 1998-02-17
CA2198951A1 (en) 1997-11-28

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC. (N.D.GES.D, US

8328 Change in the person/name/address of the agent

Representative=s name: MAIER, D., DIPL.-ING. UNIV., PAT.-ASS., 81739 MUEN