DE69633723D1 - Methode zur detektion von punktmutanten mittels nukleinsäurereperaturenzym und in vitro mutagenese - Google Patents

Methode zur detektion von punktmutanten mittels nukleinsäurereperaturenzym und in vitro mutagenese

Info

Publication number
DE69633723D1
DE69633723D1 DE69633723T DE69633723T DE69633723D1 DE 69633723 D1 DE69633723 D1 DE 69633723D1 DE 69633723 T DE69633723 T DE 69633723T DE 69633723 T DE69633723 T DE 69633723T DE 69633723 D1 DE69633723 D1 DE 69633723D1
Authority
DE
Germany
Prior art keywords
nucleic acid
vitro mutagenesis
detecting point
point mutants
repair cycle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69633723T
Other languages
English (en)
Other versions
DE69633723T2 (de
Inventor
G Chirikjian
G Collier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Trevigen Inc
Original Assignee
Trevigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/483,089 external-priority patent/US5656430A/en
Application filed by Trevigen Inc filed Critical Trevigen Inc
Application granted granted Critical
Publication of DE69633723D1 publication Critical patent/DE69633723D1/de
Publication of DE69633723T2 publication Critical patent/DE69633723T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69633723T 1995-06-07 1996-06-07 Methode zur detektion von punktmutanten mittels nukleinsäurereperaturenzym und in vitro mutagenese Expired - Fee Related DE69633723T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/483,089 US5656430A (en) 1995-06-07 1995-06-07 Oscillating signal amplifier for nucleic acid detection
US483089 1995-06-07
US1295096P 1996-03-06 1996-03-06
US12950P 1996-03-06
PCT/US1996/008694 WO1996040902A1 (en) 1995-06-07 1996-06-07 Nucleic acid repair enzyme methods for point mutation detection and in vitro mutagenesis

Publications (2)

Publication Number Publication Date
DE69633723D1 true DE69633723D1 (de) 2004-12-02
DE69633723T2 DE69633723T2 (de) 2006-02-02

Family

ID=26684228

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69633723T Expired - Fee Related DE69633723T2 (de) 1995-06-07 1996-06-07 Methode zur detektion von punktmutanten mittels nukleinsäurereperaturenzym und in vitro mutagenese

Country Status (5)

Country Link
EP (1) EP0832210B1 (de)
JP (1) JPH11506932A (de)
AU (1) AU6252796A (de)
DE (1) DE69633723T2 (de)
WO (1) WO1996040902A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6548247B1 (en) 1997-04-16 2003-04-15 Trevigen, Inc. Detection and mapping of point mutations using partial digestion
US6969584B2 (en) * 1997-06-12 2005-11-29 Rigel Pharmaceuticals, Inc. Combinatorial enzymatic complexes
WO1999042595A1 (en) * 1998-02-19 1999-08-26 Trevigen, Inc. Mismatch cleavage enzymes from extreme thermophiles and uses thereof
US6403309B1 (en) * 1999-03-19 2002-06-11 Valigen (Us), Inc. Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays
WO2001036679A2 (en) * 1999-11-15 2001-05-25 Hartwell John G METHODS FOR GENERATING SINGLE STRANDED cDNA FRAGMENTS
EP1130119A1 (de) * 2000-02-17 2001-09-05 Amsterdam Support Diagnostics B.V. Reduktion des Hintergrunds in Hybridisierungsverfahren
CA2332729A1 (en) 2000-02-17 2001-08-17 Amsterdam Support Diagnostics B.V. Reducing background in hybridisation reactions
JP4689940B2 (ja) 2001-02-02 2011-06-01 ノビシ バイオテック，リミティド ライアビリティ カンパニー ヘテロ二本鎖の相補性を増大させる方法
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
US7838219B2 (en) 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
US20120076871A1 (en) 2010-09-24 2012-03-29 Genomic Vision Sa Method for detecting, quantifying and mapping damage and/or repair of dna strands
EP2975124B1 (de) * 2013-03-14 2019-03-06 Takara Bio Inc. Verfahren zur verwendung von wärmebeständiger mismatch-endonuklease
WO2023196499A1 (en) * 2022-04-07 2023-10-12 Twist Bioscience Corporation Substrate cleavage for nucleic acid synthesis

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
AU3919293A (en) * 1992-03-27 1993-11-08 University Of Maryland At Baltimore Detection of gene mutations with mismatch repair enzymes
FR2709761B1 (fr) * 1993-09-10 1995-11-24 Pasteur Institut Procédé de détection de molécules contenant des mésappariements nucléotidiques et de localisation de ces mésappariements, et application à la détection de substitutions ou de délétions de bases .
AU8102694A (en) * 1993-11-17 1995-06-06 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences

Also Published As

Publication number Publication date
EP0832210A1 (de) 1998-04-01
DE69633723T2 (de) 2006-02-02
JPH11506932A (ja) 1999-06-22
AU6252796A (en) 1996-12-30
WO1996040902A1 (en) 1996-12-19
EP0832210B1 (de) 2004-10-27

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