DE69605206T2 - Eine kontrolle für die pcr - Google Patents

Eine kontrolle für die pcr

Info

Publication number
DE69605206T2
DE69605206T2 DE69605206T DE69605206T DE69605206T2 DE 69605206 T2 DE69605206 T2 DE 69605206T2 DE 69605206 T DE69605206 T DE 69605206T DE 69605206 T DE69605206 T DE 69605206T DE 69605206 T2 DE69605206 T2 DE 69605206T2
Authority
DE
Germany
Prior art keywords
pcr
positive control
detection
control
reagents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69605206T
Other languages
English (en)
Other versions
DE69605206D1 (de
Inventor
Bridget Andaloro
William Barbour
Robert King
Alexander Kopatsis
George Tice
Douglas Amorese
Raymond Jackson
Lisa Ecret
Heidi Zanecosky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of DE69605206D1 publication Critical patent/DE69605206D1/de
Application granted granted Critical
Publication of DE69605206T2 publication Critical patent/DE69605206T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Control Of High-Frequency Heating Circuits (AREA)
  • Control Of Temperature (AREA)
DE69605206T 1995-09-20 1996-09-19 Eine kontrolle für die pcr Expired - Fee Related DE69605206T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US406395P 1995-09-20 1995-09-20
PCT/US1996/015085 WO1997011197A1 (en) 1995-09-20 1996-09-19 Control for pcr

Publications (2)

Publication Number Publication Date
DE69605206D1 DE69605206D1 (de) 1999-12-23
DE69605206T2 true DE69605206T2 (de) 2000-05-11

Family

ID=21708953

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69605206T Expired - Fee Related DE69605206T2 (de) 1995-09-20 1996-09-19 Eine kontrolle für die pcr

Country Status (6)

Country Link
EP (1) EP0851941B1 (de)
JP (1) JPH11512602A (de)
AT (1) ATE186750T1 (de)
CA (1) CA2231184A1 (de)
DE (1) DE69605206T2 (de)
WO (1) WO1997011197A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2255760A1 (en) * 1996-05-17 1997-11-27 Susan Yen-Tee Tseng Identification of target bacteria by fluorescence detection of primer directed amplification products
JP3628232B2 (ja) * 2000-03-31 2005-03-09 三洋電機株式会社 微生物識別方法、微生物識別装置、微生物識別用データベースの作成方法および微生物識別プログラムを記録した記録媒体
US8846349B2 (en) 2009-07-22 2014-09-30 E.I. Du Pont De Nemours And Company Sequences and their use for detection and characterization of E. coli O157:H7
BR112014007394A2 (pt) 2011-09-28 2018-08-28 Du Pont método para detectar a presença de bactérias, polinucleotídeo isolado, kit e comprimido reagente
WO2013070662A1 (en) 2011-11-09 2013-05-16 E.I. Du Pont De Nemours And Company Sequences and their use for detection of salmonella
EP2959012B1 (de) 2013-02-21 2018-02-14 Qualicon Diagnostics LLC Sequenzen und deren verwendung für den nachweis und die charakterisierung von e.coli o157:h7
AU2014309047A1 (en) 2013-08-20 2016-02-11 Qualicon Diagnostics, Llc Sequences and their use for detection of Salmonella enteritidis and/or Salmonella typhimurium
MX2018008742A (es) 2018-07-16 2020-01-17 Sigma Alimentos Sa De Cv Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas.
WO2020051301A1 (en) 2018-09-06 2020-03-12 Hygiena, Llc Sequences and their use for detection and characterization of escherichia coli serotype o157:h7
US11634782B2 (en) 2019-03-20 2023-04-25 Hygiena, Llc Quantification of microorganisms in samples and methods of determining quantification conditions thereof
WO2020205491A1 (en) 2019-04-05 2020-10-08 Hygiena, Llc Sequences and their use for detection and characterization of genus cronobacter

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE142272T1 (de) * 1989-01-19 1996-09-15 Behringwerke Ag Nukleinsäure-amplifikation unter verwendung eines einzelprimers
EP0586112A3 (en) * 1992-08-14 1994-09-14 Pharma Gen S A Control of pcr mediated detection of micro-organisms
ZA936015B (en) * 1992-08-24 1994-03-10 Akzo Nv Elimination of false negatives in nuleic acid detection.
WO1994016107A1 (en) * 1993-01-12 1994-07-21 Thomas Jefferson University DNA SEQUENCING WITH Bst POLYMERASE
US5307640A (en) * 1993-01-25 1994-05-03 E. I. Du Pont De Nemours And Company Apparatus and method for producing frozen particles of a liquid

Also Published As

Publication number Publication date
CA2231184A1 (en) 1997-03-27
ATE186750T1 (de) 1999-12-15
DE69605206D1 (de) 1999-12-23
JPH11512602A (ja) 1999-11-02
WO1997011197A1 (en) 1997-03-27
EP0851941A1 (de) 1998-07-08
EP0851941B1 (de) 1999-11-17

Similar Documents

Publication Publication Date Title
AU4969996A (en) Methods for the isothermal amplification of nucleic acid molecules
ATE210192T1 (de) VERFAHREN ZUR DIREKTEN SEQUENZIERUNG WÄHREND ßTEMPLATEß-AMPLIFIKATION
FR2685346B1 (fr) Procede de preparation d'arn double-brin, et ses applications.
PL307341A1 (en) Method of as well as reagent and kit for detecting and amplifying a sequence of nucleic acids
WO2000055368A3 (en) Probe/mobility modifier complexes for multiplex nucleic acid detection
ES2101867T3 (es) Aplicaciones diagnosticas de la formacion de lazos d dobles.
WO1999014377A3 (en) Detection and identification of human papillomavirus by pcr and type-specific reverse hybridization
ATE186750T1 (de) Eine kontrolle für die pcr
EP0714987A3 (de) Verfahren zur Quantifizierung von genomischer DNA
DE69427103T2 (de) Verfahren, Reagenzien und Kits zur Untersuchung von Neisseria gonorrhoeae und Chlamydia trachomatis
EP0822256A3 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
AU7492894A (en) Improved method for the quantification of nucleic acid
EP0745675A3 (de) Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür
DE69627625T2 (de) Die amplifizierung und bestimmung von arten des mycobakterium avium-komplexes
ES2186745T3 (es) Procedimiento para la identificacion especifica en genero y especie de legionelas.
EP0128018A3 (en) Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
WO1998011259A3 (en) Composition, method and kit for detection and identification of sexually transmitted disease microorganisms
WO1996015264A3 (en) Method for identifying nucleic acid sequences from different biological sources through amplification of the same
DE69604051T2 (de) Verfahren zum sensitiven nachweis von reverser transcriptase
DE59608745D1 (de) Verfahren zur genomanalyse und mittel zur durchführung des verfahrens
EP0919633A3 (de) Detektion von Neisseria Gonorrhoeae mittels Amplifizierung und Detektion der entsprechenden Nukleinsäuren
AU4397101A (en) Method and kit for the detection of schistosomiasis through the polymerase chainreaction
AU3535389A (en) Method for diagnosis of infectious diseases and method of detecting and identifying microorganisms
AU5026196A (en) Methods and reagents for typing hla class i genes
CA2211165A1 (en) Methods for sensitive detection of reverse transcriptase

Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee