DE69530194D1 - Chimäre delta-endotoxin exprimierung in pseudomonas fluoreszens - Google Patents
Chimäre delta-endotoxin exprimierung in pseudomonas fluoreszensInfo
- Publication number
- DE69530194D1 DE69530194D1 DE69530194T DE69530194T DE69530194D1 DE 69530194 D1 DE69530194 D1 DE 69530194D1 DE 69530194 T DE69530194 T DE 69530194T DE 69530194 T DE69530194 T DE 69530194T DE 69530194 D1 DE69530194 D1 DE 69530194D1
- Authority
- DE
- Germany
- Prior art keywords
- protoxin
- segment
- expression
- chimeral
- delta
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000002158 endotoxin Substances 0.000 title abstract 3
- 241000589540 Pseudomonas fluorescens Species 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 241000193388 Bacillus thuringiensis Species 0.000 abstract 3
- 101100007609 Bacillus thuringiensis subsp. aizawai cry1Fa gene Proteins 0.000 abstract 3
- 229940097012 bacillus thuringiensis Drugs 0.000 abstract 3
- 101150065438 cry1Ab gene Proteins 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/239,476 US5527883A (en) | 1994-05-06 | 1994-05-06 | Delta-endotoxin expression in pseudomonas fluorescens |
PCT/US1995/005431 WO1995030753A1 (en) | 1994-05-06 | 1995-05-05 | CHIMERIC DELTA-ENDOTOXIN EXPRESSION IN $i(PSEUDOMONAS FLUORESCENS) |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69530194D1 true DE69530194D1 (de) | 2003-05-08 |
DE69530194T2 DE69530194T2 (de) | 2003-11-13 |
Family
ID=22902310
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69530194T Expired - Fee Related DE69530194T2 (de) | 1994-05-06 | 1995-05-05 | Chimäre delta-endotoxin exprimierung in pseudomonas fluoreszens |
Country Status (11)
Country | Link |
---|---|
US (2) | US5527883A (de) |
EP (1) | EP0758385B1 (de) |
JP (1) | JP3878209B2 (de) |
KR (1) | KR100376096B1 (de) |
AT (1) | ATE236253T1 (de) |
AU (1) | AU697148B2 (de) |
CA (1) | CA2188795A1 (de) |
DE (1) | DE69530194T2 (de) |
ES (1) | ES2194909T3 (de) |
NZ (1) | NZ285524A (de) |
WO (1) | WO1995030753A1 (de) |
Families Citing this family (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6780408B1 (en) | 1993-09-02 | 2004-08-24 | Syngenta Participations Ag | Genes encoding hybrid bacillus thuringiensis toxins |
GB9318207D0 (en) | 1993-09-02 | 1993-10-20 | Sandoz Ltd | Improvements in or relating to organic compounds |
US5527883A (en) * | 1994-05-06 | 1996-06-18 | Mycogen Corporation | Delta-endotoxin expression in pseudomonas fluorescens |
US5508264A (en) * | 1994-12-06 | 1996-04-16 | Mycogen Corporation | Pesticidal compositions |
US5670365A (en) * | 1995-10-06 | 1997-09-23 | Mycogen Corporation | Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates |
ES2161096B1 (es) * | 1996-03-08 | 2003-02-16 | Univ Catalunya Politecnica | Aparato para la determinacion de la resistencia al paso de liquidos enlos tejidos. |
US6063756A (en) | 1996-09-24 | 2000-05-16 | Monsanto Company | Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor |
US6110668A (en) * | 1996-10-07 | 2000-08-29 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Gene synthesis method |
US5965428A (en) * | 1996-10-08 | 1999-10-12 | Ecogen, Inc. | Chimeric lepidopteran-toxic crystal proteins |
US6713063B1 (en) | 1996-11-20 | 2004-03-30 | Monsanto Technology, Llc | Broad-spectrum δ-endotoxins |
US6017534A (en) * | 1996-11-20 | 2000-01-25 | Ecogen, Inc. | Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity |
ID25530A (id) | 1996-11-20 | 2000-10-12 | Ecogen Inc | δ-ENDOTOKSIN BERSPEKTRUM-LEBAR |
US6121436A (en) | 1996-12-13 | 2000-09-19 | Monsanto Company | Antifungal polypeptide and methods for controlling plant pathogenic fungi |
JP2001519663A (ja) * | 1997-04-04 | 2001-10-23 | ピー. ウィークス、ドナルド | 遺伝子導入ジカンバ−分解生物体を製造及び使用するための方法及び材料 |
EP1676922B1 (de) * | 1997-11-12 | 2008-09-17 | Mycogen Corporation | Pflanzen-optimierte Gene, welche für Toxine mit pestizider Wirkung kodieren |
US6218188B1 (en) * | 1997-11-12 | 2001-04-17 | Mycogen Corporation | Plant-optimized genes encoding pesticidal toxins |
AU774176B2 (en) * | 1997-11-12 | 2004-06-17 | Mycogen Corporation | Plant-optimised genes encoding pesticidal toxins |
AU4463000A (en) * | 1999-04-21 | 2000-11-02 | University Of Florida Research Foundation, Inc. | Neuropeptides and their use for pest control |
US6593299B1 (en) | 1999-04-21 | 2003-07-15 | University Of Florida Research Foundation, Inc. | Compositions and methods for controlling pests |
AU2002306484A1 (en) | 2002-02-13 | 2003-09-04 | Dow Global Technologies Inc. | Over-expression of extremozyme genes in pseudomonads and closely related bacteria |
JP4472998B2 (ja) * | 2002-04-22 | 2010-06-02 | ダウ グローバル テクノロジーズ インコーポレイティド | 低コストのペプチド製造 |
US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
JP5087741B2 (ja) | 2003-11-19 | 2012-12-05 | フェネックス インコーポレイテッド | 改良タンパク質発現系 |
CA2546157C (en) | 2003-11-21 | 2014-07-22 | Dow Global Technolgies Inc. | Improved expression systems with sec-system secretion |
US20050214321A1 (en) * | 2003-12-01 | 2005-09-29 | Dow Global Technologies Inc. | Recombinant icosahedral virus like particle production in pseudomonads |
KR101183720B1 (ko) | 2004-01-16 | 2012-09-17 | 다우 글로벌 테크놀로지스 엘엘씨 | 슈도모나스 플루오레센스에서의 포유류 단백질의 발현 |
JP2008507294A (ja) | 2004-07-26 | 2008-03-13 | ダウ グローバル テクノロジーズ インコーポレイティド | 菌株遺伝子操作による改善されたタンパク質発現のための方法 |
WO2007027777A2 (en) | 2005-08-31 | 2007-03-08 | Monsanto Technology Llc | Nucleotide sequences encoding insecticidal proteins |
CN101495635B (zh) | 2006-05-26 | 2017-05-17 | 孟山都技术有限公司 | 对应于转基因事件mon89034的玉米植物和种子及其检测和使用方法 |
EP2468869B1 (de) | 2007-01-31 | 2015-03-18 | Pfenex Inc. | Bakterielle Leader-Sequenzen für erhöhte Expression |
CN101784655A (zh) * | 2007-04-27 | 2010-07-21 | 菲尼克斯股份有限公司 | 可溶性重组二十面体病毒样颗粒的改良生成和体内装配 |
AU2008245696B2 (en) | 2007-04-27 | 2013-11-07 | Pelican Technology Holdings, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
WO2009124012A1 (en) * | 2008-03-31 | 2009-10-08 | Dow Global Technologies Inc. | A design for rapidly cloning one or more polypeptide chains into an expression system |
US9243253B2 (en) | 2009-05-11 | 2016-01-26 | Pfenex, Inc. | Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein |
CA2782627A1 (en) * | 2009-12-16 | 2011-06-23 | Dow Agrosciences Llc | Combined use of cry1fa and cry1i for management of spodoptera fugiperda and ostrinia nubilalis |
UA111936C2 (uk) | 2009-12-16 | 2016-07-11 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | ТРАНСГЕННА РОСЛИНА, ЯКА МІСТИТЬ ДНК, ЩО КОДУЄ ІНСЕКТИЦИДНИЙ БІЛОК Vip3Ab, І ДНК, ЩО КОДУЄ ІНСЕКТИЦИДНИЙ БІЛОК Cry1Ca, ДЛЯ КЕРУВАННЯ РЕЗИСТЕНТНІСТЮ КОМАХ |
CA2844913A1 (en) | 2011-08-19 | 2013-02-28 | Synthetic Genomics, Inc. | Integrated method for high-throughput identification of novel pesticidal compositions and uses therefor |
CA2886787A1 (en) * | 2012-10-05 | 2014-04-10 | Dow Agrosciences Llc | Use of cry1ea in combinations for management of resistant fall armyworm insects |
WO2016057123A1 (en) | 2014-08-28 | 2016-04-14 | Dow Agrosciences Llc | Dig-17 insecticidal cry toxins |
EP3240560B1 (de) | 2014-12-30 | 2022-08-31 | Corteva Agriscience LLC | Modifizierte cry1ca-toxine zur bekämpfung von insektenschädlingen |
US10731176B2 (en) * | 2014-12-30 | 2020-08-04 | Dow Agrosciences Llc | DIG-305 insecticidal Cry toxins |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11714090B2 (en) | 2017-12-15 | 2023-08-01 | Syngenta Participations Ag | Non-antibody ligands for detecting target proteins |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4448885A (en) * | 1981-04-27 | 1984-05-15 | Board Of The Regents Of The University Of Washington | Bacillus thuringiensis crystal protein in Escherichia coli |
US4467036A (en) * | 1981-11-12 | 1984-08-21 | The Board Of Regents Of The University Of Washington | Bacillus thuringiensis crystal protein in Escherichia coli |
US4797276A (en) * | 1985-03-22 | 1989-01-10 | Mycogen Corporation | Control of cotton boll weevil, alfalfa weevil, and corn rootworm via contact with a strain of Bacillus thuringiensis |
US4918006A (en) * | 1985-07-01 | 1990-04-17 | E. I. Du Pont De Nemours And Company | Gene coding for insecticidal crystal protein |
US4853331A (en) * | 1985-08-16 | 1989-08-01 | Mycogen Corporation | Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera |
US5151363A (en) * | 1990-07-27 | 1992-09-29 | Mycogen Corporation | Isolates of Bacillus thuringiensis that are active against nematodes |
US4948734A (en) * | 1987-08-12 | 1990-08-14 | Mycogen Corporation | Novel isolates of bacillus thuringiensis having activity against nematodes |
US4849217A (en) * | 1987-11-19 | 1989-07-18 | Mycogen Corporation | Novel isolates of bacilus thuringiensis having activity against the alfalfa weevil, hypera brunneipennis |
US5128130A (en) * | 1988-01-22 | 1992-07-07 | Mycogen Corporation | Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens |
US5055294A (en) * | 1988-03-03 | 1991-10-08 | Mycogen Corporation | Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens |
US5169760A (en) * | 1989-07-27 | 1992-12-08 | Mycogen Corporation | Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters |
US5187091A (en) * | 1990-03-20 | 1993-02-16 | Ecogen Inc. | Bacillus thuringiensis cryiiic gene encoding toxic to coleopteran insects |
EP0471564A3 (en) * | 1990-08-16 | 1992-11-25 | Mycogen Corporation | Translational enhancement in pseudomonas |
US5208077A (en) * | 1990-11-09 | 1993-05-04 | Florida Wire And Cable Company | Method for a composite material comprising coated and filled metal strand for use in prestressed concrete, stay cables for cable-stayed bridges and other uses |
US5436002A (en) * | 1991-01-29 | 1995-07-25 | Mycogen Corporation | Bacillus thuringiensisisolate PS201T6 toxin |
US5350576A (en) * | 1991-09-13 | 1994-09-27 | Mycogen Corporation | Bacillus thuringiensis isolates for controlling acarides |
US5441884A (en) * | 1993-07-08 | 1995-08-15 | Ecogen Inc. | Bacillus thuringiensis transposon TN5401 |
US5593881A (en) * | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
US5527883A (en) * | 1994-05-06 | 1996-06-18 | Mycogen Corporation | Delta-endotoxin expression in pseudomonas fluorescens |
-
1994
- 1994-05-06 US US08/239,476 patent/US5527883A/en not_active Expired - Lifetime
-
1995
- 1995-05-05 DE DE69530194T patent/DE69530194T2/de not_active Expired - Fee Related
- 1995-05-05 AU AU24652/95A patent/AU697148B2/en not_active Expired
- 1995-05-05 CA CA002188795A patent/CA2188795A1/en not_active Abandoned
- 1995-05-05 EP EP95918907A patent/EP0758385B1/de not_active Expired - Lifetime
- 1995-05-05 AT AT95918907T patent/ATE236253T1/de not_active IP Right Cessation
- 1995-05-05 WO PCT/US1995/005431 patent/WO1995030753A1/en active IP Right Grant
- 1995-05-05 JP JP52904695A patent/JP3878209B2/ja not_active Expired - Lifetime
- 1995-05-05 NZ NZ285524A patent/NZ285524A/en not_active IP Right Cessation
- 1995-05-05 KR KR1019960706280A patent/KR100376096B1/ko not_active IP Right Cessation
- 1995-05-05 ES ES95918907T patent/ES2194909T3/es not_active Expired - Lifetime
-
1996
- 1996-04-24 US US08/639,923 patent/US5840554A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JPH10500292A (ja) | 1998-01-13 |
KR970702925A (ko) | 1997-06-10 |
KR100376096B1 (ko) | 2003-10-10 |
AU2465295A (en) | 1995-11-29 |
ES2194909T3 (es) | 2003-12-01 |
WO1995030753A1 (en) | 1995-11-16 |
US5527883A (en) | 1996-06-18 |
AU697148B2 (en) | 1998-10-01 |
DE69530194T2 (de) | 2003-11-13 |
ATE236253T1 (de) | 2003-04-15 |
JP3878209B2 (ja) | 2007-02-07 |
CA2188795A1 (en) | 1995-11-16 |
EP0758385A1 (de) | 1997-02-19 |
NZ285524A (en) | 1998-09-24 |
EP0758385B1 (de) | 2003-04-02 |
US5840554A (en) | 1998-11-24 |
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