ES2060753T3 - Nuevo gen hibrido de bacillus thuringiensis, plasmido y pseudomonas fluorescens transformado. - Google Patents

Nuevo gen hibrido de bacillus thuringiensis, plasmido y pseudomonas fluorescens transformado.

Info

Publication number
ES2060753T3
ES2060753T3 ES89302049T ES89302049T ES2060753T3 ES 2060753 T3 ES2060753 T3 ES 2060753T3 ES 89302049 T ES89302049 T ES 89302049T ES 89302049 T ES89302049 T ES 89302049T ES 2060753 T3 ES2060753 T3 ES 2060753T3
Authority
ES
Spain
Prior art keywords
bacillus thuringiensis
plasmid
pseudomonas fluorescens
hybrid gene
new hybrid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89302049T
Other languages
English (en)
Inventor
Thomas E Gilroy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mycogen Corp
Original Assignee
Mycogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mycogen Corp filed Critical Mycogen Corp
Application granted granted Critical
Publication of ES2060753T3 publication Critical patent/ES2060753T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

SE HA CLONADO EL GEN DE LA TOXINA HIBRIDA DE BACILLUS THURINGIENSIS TOXICO PARA INSECTOS LEPIDOPTEROS. EL DNA QUE CODIFICA LA TOXINA DEL BACILLUS THURINGIENSIS PUEDE SER USADO PARA TRANSFORMAR VARIOS MICROBIOS PROCARIOTICOS Y EUCARIOTICOS PARA EXPRESAR LA TOXINA DEL BACILLUS THURINGIENSIS. ESTOS MICROBIOS RECOMBINANTES PUEDEN EMPLEARSE PARA CONTROLAR INSECTOS LEPIDOPTEROS EN DIVERSOS AMBIENTES.
ES89302049T 1988-03-03 1989-03-01 Nuevo gen hibrido de bacillus thuringiensis, plasmido y pseudomonas fluorescens transformado. Expired - Lifetime ES2060753T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/164,162 US5055294A (en) 1988-03-03 1988-03-03 Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens

Publications (1)

Publication Number Publication Date
ES2060753T3 true ES2060753T3 (es) 1994-12-01

Family

ID=22593256

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89302049T Expired - Lifetime ES2060753T3 (es) 1988-03-03 1989-03-01 Nuevo gen hibrido de bacillus thuringiensis, plasmido y pseudomonas fluorescens transformado.

Country Status (7)

Country Link
US (1) US5055294A (es)
EP (1) EP0331470B1 (es)
JP (1) JP3298103B2 (es)
AT (1) ATE112315T1 (es)
CA (1) CA1341153C (es)
DE (1) DE68918477T2 (es)
ES (1) ES2060753T3 (es)

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CA2012025C (en) * 1989-03-14 1999-09-07 Yukiko Tsukuda Dna for expression and secretion
US5593881A (en) * 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5527883A (en) * 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US5702703A (en) * 1994-11-16 1997-12-30 Mycogen Corporation Bacillus thuringiensis toxin enhancer
US5508264A (en) * 1994-12-06 1996-04-16 Mycogen Corporation Pesticidal compositions
DE69638032D1 (de) * 1995-10-13 2009-11-05 Dow Agrosciences Llc Modifiziertes bacillus thuringiensis gen zur kontrolle von lepidoptera in pflanzen
US5965428A (en) * 1996-10-08 1999-10-12 Ecogen, Inc. Chimeric lepidopteran-toxic crystal proteins
CA2272843C (en) * 1996-11-20 2009-11-10 Ecogen, Inc. Broad-spectrum delta-endotoxins
US6017534A (en) * 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
US6713063B1 (en) * 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
SK13062001A3 (sk) 1999-03-30 2002-05-09 Agraquest, Inc. Biologicky čistá kultúra, prostriedok, izolovaný metabolit, supernatant, čiastočne vyčistená frakcia, vo vode rozpustná látka, spôsob produkcie fungicídneho supernatantu, spôsob čiastočného vyčistenia supernatantu, spôsob zvýšenia insekticídnej aktivity
US6245551B1 (en) 1999-03-30 2001-06-12 Agraquest, Inc. Strain of Bacillus pumilus for controlling plant diseases caused by fungi
AU2002306484A1 (en) 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
US7053266B2 (en) * 2002-03-27 2006-05-30 Council Of Scientfic And Industrial Research Chimeric cry1E δendotoxin and methods of controlling insects
US7557186B2 (en) 2002-03-27 2009-07-07 Council Of Scientific & Industrial Research Chimeric CrylE δ endotoxin and methods of controlling insects
KR20050016362A (ko) * 2002-04-22 2005-02-21 다우 글로벌 테크놀로지스 인크. 펩티드의 저비용 생산
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
CA2545610C (en) 2003-11-19 2014-03-25 Dow Global Technolgies Inc. Auxotrophic pseudomonas fluorescens bacteria for recombinant protein expression
EP2314602A1 (en) 2003-11-21 2011-04-27 Pfenex, Inc. Improved expression systems with SEC-system secretion
CN101151272A (zh) * 2003-12-01 2008-03-26 陶氏环球技术公司 在假单胞菌中生产重组二十面体病毒样颗粒
KR101183720B1 (ko) 2004-01-16 2012-09-17 다우 글로벌 테크놀로지스 엘엘씨 슈도모나스 플루오레센스에서의 포유류 단백질의 발현
CA2574953A1 (en) 2004-07-26 2006-02-09 Dow Global Technolgies Inc. Process for improved protein expression by strain engineering
CA2771677A1 (en) * 2005-08-31 2007-03-08 Monsanto Technology Llc Nucleotide sequences encoding insecticidal proteins
UA98770C2 (ru) 2006-05-26 2012-06-25 Монсанто Текнолоджи, Ллс Растение кукурузы и семена, которые соответствуют трансгенному случаю mon89034, и способы его обнаружения и применения
BRPI0807834A2 (pt) 2007-01-31 2014-07-22 Dow Global Technologies Inc " sequências-líder bacterianas para expressão aumentada ".
WO2008134461A2 (en) 2007-04-27 2008-11-06 Dow Global Technologies, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2009124012A1 (en) * 2008-03-31 2009-10-08 Dow Global Technologies Inc. A design for rapidly cloning one or more polypeptide chains into an expression system
EP2430164B1 (en) 2009-05-11 2017-07-19 Pfenex, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
AU2011223627B2 (en) 2010-03-04 2015-06-18 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
MX343356B (es) * 2010-03-30 2016-11-03 Pfenex Inc * Toxinas proteicas recombinantes de alto nivel de expresion.
NZ603033A (en) 2010-04-01 2014-06-27 Pfenex Inc Methods for g-csf production in a pseudomonas host cell
EP3766978A1 (en) 2012-03-09 2021-01-20 Vestaron Corporation Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
MX2017006506A (es) 2014-11-20 2018-03-12 Biological E Ltd Polinucleotido optimizado en el codon para la expresion de alto nivel de crm197.
RU2017123283A (ru) 2014-12-01 2019-01-09 Пфенекс Инк. Партнеры по слиянию для получения пептидов
AU2015372474B2 (en) 2014-12-30 2019-02-14 Dow Agrosciences Llc Modified Cry1Ca toxins useful for control of insect pests
US11535653B2 (en) 2016-10-21 2022-12-27 Vestaron Corporation Cleavable peptides and insecticidal and nematicidal proteins comprising same
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
WO2019083793A1 (en) 2017-10-27 2019-05-02 Pfenex Inc. PROCESS FOR THE PRODUCTION OF RECOMBINANT ERWINIA ASPARAGINASE
KR20200073280A (ko) 2017-10-27 2020-06-23 피페넥스 인크. 주변세포질 단백질 발현을 위한 박테리아 리더 서열
CN116916966A (zh) 2020-12-23 2023-10-20 爵士制药爱尔兰有限公司 纯化电荷屏蔽融合蛋白的方法
US20220313798A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4467036A (en) * 1981-11-12 1984-08-21 The Board Of Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
US4448885A (en) * 1981-04-27 1984-05-15 Board Of The Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
ATE84072T1 (de) * 1985-04-30 1993-01-15 Monsanto Co Pflanzenkolonisierende mikroorganismen, die das bacillus thuringiensis-toxingen als chromosomeinsetzung enthalten.
US4771131A (en) * 1985-08-16 1988-09-13 Mycogen Corporation Cloning and expression of Bacillus thuringiensis toxin gene encoding a protein toxic to beetles of the order Coleoptera
CA1341092C (en) * 1985-12-12 2000-09-05 David L. Edwards Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby

Also Published As

Publication number Publication date
EP0331470A3 (en) 1991-03-06
ATE112315T1 (de) 1994-10-15
US5055294A (en) 1991-10-08
DE68918477D1 (de) 1994-11-03
DE68918477T2 (de) 1995-02-16
CA1341153C (en) 2000-12-12
JP3298103B2 (ja) 2002-07-02
EP0331470A2 (en) 1989-09-06
JPH025873A (ja) 1990-01-10
EP0331470B1 (en) 1994-09-28

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