ATE236253T1 - Chimäre delta-endotoxin exprimierung in pseudomonas fluoreszens - Google Patents

Chimäre delta-endotoxin exprimierung in pseudomonas fluoreszens

Info

Publication number
ATE236253T1
ATE236253T1 AT95918907T AT95918907T ATE236253T1 AT E236253 T1 ATE236253 T1 AT E236253T1 AT 95918907 T AT95918907 T AT 95918907T AT 95918907 T AT95918907 T AT 95918907T AT E236253 T1 ATE236253 T1 AT E236253T1
Authority
AT
Austria
Prior art keywords
protoxin
segment
expression
cryif
bacillus thuringiensis
Prior art date
Application number
AT95918907T
Other languages
English (en)
Inventor
Mark Thompson
George E Schwab
Original Assignee
Mycogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mycogen Corp filed Critical Mycogen Corp
Application granted granted Critical
Publication of ATE236253T1 publication Critical patent/ATE236253T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Dentistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Pest Control & Pesticides (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT95918907T 1994-05-06 1995-05-05 Chimäre delta-endotoxin exprimierung in pseudomonas fluoreszens ATE236253T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/239,476 US5527883A (en) 1994-05-06 1994-05-06 Delta-endotoxin expression in pseudomonas fluorescens
PCT/US1995/005431 WO1995030753A1 (en) 1994-05-06 1995-05-05 CHIMERIC DELTA-ENDOTOXIN EXPRESSION IN $i(PSEUDOMONAS FLUORESCENS)

Publications (1)

Publication Number Publication Date
ATE236253T1 true ATE236253T1 (de) 2003-04-15

Family

ID=22902310

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95918907T ATE236253T1 (de) 1994-05-06 1995-05-05 Chimäre delta-endotoxin exprimierung in pseudomonas fluoreszens

Country Status (11)

Country Link
US (2) US5527883A (de)
EP (1) EP0758385B1 (de)
JP (1) JP3878209B2 (de)
KR (1) KR100376096B1 (de)
AT (1) ATE236253T1 (de)
AU (1) AU697148B2 (de)
CA (1) CA2188795A1 (de)
DE (1) DE69530194T2 (de)
ES (1) ES2194909T3 (de)
NZ (1) NZ285524A (de)
WO (1) WO1995030753A1 (de)

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US5527883A (en) * 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US5508264A (en) * 1994-12-06 1996-04-16 Mycogen Corporation Pesticidal compositions
US5670365A (en) * 1995-10-06 1997-09-23 Mycogen Corporation Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates
ES2161096B1 (es) * 1996-03-08 2003-02-16 Univ Catalunya Politecnica Aparato para la determinacion de la resistencia al paso de liquidos enlos tejidos.
US6063756A (en) 1996-09-24 2000-05-16 Monsanto Company Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
US5965428A (en) * 1996-10-08 1999-10-12 Ecogen, Inc. Chimeric lepidopteran-toxic crystal proteins
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
EP0942985B1 (de) 1996-11-20 2004-09-15 Monsanto Technology LLC Delta-endotoxine mit breitem spektrum
US6713063B1 (en) 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
JP2001519663A (ja) * 1997-04-04 2001-10-23 ピー． ウィークス、ドナルド 遺伝子導入ジカンバ−分解生物体を製造及び使用するための方法及び材料
AU774176B2 (en) * 1997-11-12 2004-06-17 Mycogen Corporation Plant-optimised genes encoding pesticidal toxins
EP1676922B1 (de) * 1997-11-12 2008-09-17 Mycogen Corporation Pflanzen-optimierte Gene, welche für Toxine mit pestizider Wirkung kodieren
US6218188B1 (en) 1997-11-12 2001-04-17 Mycogen Corporation Plant-optimized genes encoding pesticidal toxins
US6593299B1 (en) 1999-04-21 2003-07-15 University Of Florida Research Foundation, Inc. Compositions and methods for controlling pests
WO2000062615A1 (en) * 1999-04-21 2000-10-26 University Of Florida Research Foundation, Inc. Neuropeptides and their use for pest control
AU2002306484A1 (en) * 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
JP4472998B2 (ja) * 2002-04-22 2010-06-02 ダウ グローバル テクノロジーズ インコーポレイティド 低コストのペプチド製造
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
JP5087741B2 (ja) * 2003-11-19 2012-12-05 フェネックス インコーポレイテッド 改良タンパク質発現系
CN1882605B (zh) 2003-11-21 2012-07-11 陶氏环球技术公司 使用Sec-系统分泌的改进的表达系统
EP1758925A4 (de) * 2003-12-01 2009-04-08 Dow Global Technologies Inc Produktion eines rekombinanten ikosahedrischen virusähnlichen partikels
EP2434016B1 (de) * 2004-01-16 2018-11-07 Pfenex Inc. Expression von säugerproteinen in pseudomonas fluorescens
EP1774017B1 (de) 2004-07-26 2013-05-15 Pfenex Inc. Verfahren zur verbesserten proteinexpression durch strain-engineering
PL1919935T3 (pl) 2005-08-31 2013-05-31 Monsanto Technology Llc Sekwencje nukleotydowe kodujące białka owadobójcze
AU2007267586B2 (en) 2006-05-26 2012-04-05 Monsanto Technology, Llc Corn plant and seed corresponding to transgenic event MON89034 and methods for detection and use thereof
CA2677179C (en) 2007-01-31 2016-02-16 Dow Global Technologies Inc. Bacterial leader sequences for increased expression
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
CA2685308A1 (en) * 2007-04-27 2009-01-29 Dow Global Technologies Inc. Improved production and in vivo assembly of soluble recombinant icosahedral virus-like particles
US20110020830A1 (en) * 2008-03-31 2011-01-27 Schneider Jane C Design for rapidly cloning one or more polypeptide chains into an expression system
NZ596283A (en) 2009-05-11 2013-08-30 Pfenex Inc Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
UA116433C2 (uk) * 2009-12-16 2018-03-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі ТРАНСГЕННА РОСЛИНА, ЯКА ПРОДУКУЄ ІНСЕКТИЦИДНІ БІЛКИ Cry1Fa і Cry1Ea, ДЛЯ ЗАПОБІГАННЯ РОЗВИТКУ СТІЙКОСТІ У СОВКИ ТРАВ'ЯНОЇ
WO2011075588A1 (en) 2009-12-16 2011-06-23 Dow Agrosciences Llc COMBINED USE OF CRY1Ca AND CRY1Fa PROTEINS FOR INSECT RESISTANCE MANAGEMENT
CA2844913A1 (en) 2011-08-19 2013-02-28 Synthetic Genomics, Inc. Integrated method for high-throughput identification of novel pesticidal compositions and uses therefor
AR092905A1 (es) * 2012-10-05 2015-05-06 Dow Agrosciences Llc Uso de cry1ea en combinaciones para el control del insecto resistente gusano del ejercito del otoño
US10028510B2 (en) 2014-08-28 2018-07-24 Dow Agrosciences Llc DIG-17 insecticidal cry toxins
EP3240900A4 (de) * 2014-12-30 2018-06-06 Dow Agrosciences LLC Insektizide dig-305-cry-toxine
RU2017122359A (ru) 2014-12-30 2019-01-31 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Модифицированные токсины cryca, применимые для борьбы с насекомыми-вредителями
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
BR112020011768A2 (pt) 2017-12-15 2020-11-17 Syngenta Participations Ag ligantes diferentes de anticorpos para detecção de proteínas alvo

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Also Published As

Publication number Publication date
EP0758385A1 (de) 1997-02-19
DE69530194D1 (de) 2003-05-08
AU697148B2 (en) 1998-10-01
KR100376096B1 (ko) 2003-10-10
JPH10500292A (ja) 1998-01-13
KR970702925A (ko) 1997-06-10
AU2465295A (en) 1995-11-29
EP0758385B1 (de) 2003-04-02
DE69530194T2 (de) 2003-11-13
ES2194909T3 (es) 2003-12-01
US5840554A (en) 1998-11-24
JP3878209B2 (ja) 2007-02-07
US5527883A (en) 1996-06-18
WO1995030753A1 (en) 1995-11-16
CA2188795A1 (en) 1995-11-16
NZ285524A (en) 1998-09-24

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