DE69434542D1 - Dna-sequenzierung mit hoher auflösung mittels einem medium geringer viskosität - Google Patents

Dna-sequenzierung mit hoher auflösung mittels einem medium geringer viskosität

Info

Publication number
DE69434542D1
DE69434542D1 DE69434542T DE69434542T DE69434542D1 DE 69434542 D1 DE69434542 D1 DE 69434542D1 DE 69434542 T DE69434542 T DE 69434542T DE 69434542 T DE69434542 T DE 69434542T DE 69434542 D1 DE69434542 D1 DE 69434542D1
Authority
DE
Germany
Prior art keywords
dna sequencing
medium
high resolution
low viscosity
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69434542T
Other languages
English (en)
Other versions
DE69434542T2 (de
Inventor
D Grossman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Publication of DE69434542D1 publication Critical patent/DE69434542D1/de
Application granted granted Critical
Publication of DE69434542T2 publication Critical patent/DE69434542T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69434542T 1993-01-21 1994-01-12 Dna-sequenzierung mit hoher auflösung mittels einem medium geringer viskosität Expired - Lifetime DE69434542T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3968 1993-01-21
US08/003,968 US5374527A (en) 1993-01-21 1993-01-21 High resolution DNA sequencing method using low viscosity medium
PCT/US1994/000394 WO1994017207A1 (en) 1993-01-21 1994-01-12 High resolution dna sequencing method using low viscosity medium

Publications (2)

Publication Number Publication Date
DE69434542D1 true DE69434542D1 (de) 2005-12-22
DE69434542T2 DE69434542T2 (de) 2006-08-10

Family

ID=21708454

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434542T Expired - Lifetime DE69434542T2 (de) 1993-01-21 1994-01-12 Dna-sequenzierung mit hoher auflösung mittels einem medium geringer viskosität

Country Status (8)

Country Link
US (1) US5374527A (de)
EP (1) EP0680516B1 (de)
JP (3) JPH08509596A (de)
AT (1) ATE310102T1 (de)
AU (1) AU674390B2 (de)
CA (1) CA2153991C (de)
DE (1) DE69434542T2 (de)
WO (1) WO1994017207A1 (de)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2155186A1 (en) * 1993-02-01 1994-08-18 Kevin M. Ulmer Methods and apparatus for dna sequencing
US5891313A (en) * 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
US6358385B1 (en) * 1993-12-17 2002-03-19 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
AU1254295A (en) * 1993-12-17 1995-07-03 Perkin-Elmer Corporation, The Uncharged polymers for separation of biomolecules by capillary electrophoresis
AU5539096A (en) * 1995-04-12 1996-11-07 Seurat Analytical Systems Incorporated Nucleic acid sequencing
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5534123A (en) * 1995-07-10 1996-07-09 Molecular Dynamics Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis
US6193866B1 (en) 1996-03-27 2001-02-27 Curagen Corporation Separation of charged particles by a spatially and temporally varying electric field
US5938904A (en) * 1996-03-27 1999-08-17 Curagen Corporation Separation of charged particles by a spatially and temporally varying electric field
US5846727A (en) * 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
US6309829B1 (en) 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US5958694A (en) * 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US5936087A (en) 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US5948227A (en) * 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
CA2328881A1 (en) 1998-04-16 1999-10-21 Northeastern University Expert system for analysis of dna sequencing electropherograms
US6096875A (en) 1998-05-29 2000-08-01 The Perlein-Elmer Corporation Nucleotide compounds including a rigid linker
SE9802558D0 (sv) 1998-07-16 1998-07-16 Hanning Instr Ab Device for detection of fluorescent
US6263286B1 (en) * 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
AU5923099A (en) * 1998-09-16 2000-04-03 Glaxo Group Limited Multiple sequencing method
EP2278320B1 (de) 1999-02-02 2018-07-18 Caliper Life Sciences, Inc. Verfahren und Vorrichtungen zur Charakterisierung von Proteinen
US6716948B1 (en) 1999-07-31 2004-04-06 Symyx Technologies, Inc. Controlled-architecture polymers and use thereof as separation media
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6741983B1 (en) * 1999-09-28 2004-05-25 John D. Birdwell Method of indexed storage and retrieval of multidimensional information
US6807490B1 (en) * 2000-02-15 2004-10-19 Mark W. Perlin Method for DNA mixture analysis
US7255999B2 (en) 2001-05-21 2007-08-14 Monogram Biosciences, Inc. Methods and compositions for analyzing proteins
DE60106053T2 (de) * 2000-07-21 2005-11-17 Aclara BioSciences, Inc., Mountain View Verfahren und vorrichtungen zur kapillarelektrophorese mit einer norbornenhaltigen oberflächenbeschichtung
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6582577B1 (en) 2000-08-31 2003-06-24 Visible Genetics Inc. Electrophoresis gel cassette
US6706162B1 (en) 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
JP4741170B2 (ja) * 2000-10-11 2011-08-03 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー アニオン性リンカーを有する蛍光核酸塩基結合体
US6797139B2 (en) 2001-03-19 2004-09-28 Applera Corporation Detection cell for guiding excitation light therein and method for using same
US6596140B2 (en) * 2001-05-01 2003-07-22 Applera Corporation Multi-channel capillary electrophoresis device and method
US6902657B2 (en) * 2001-06-15 2005-06-07 Amersham Biosciences Corp. Electrophoresis separation media and methods
US8697029B2 (en) * 2002-04-18 2014-04-15 The Regents Of The University Of Michigan Modulated physical and chemical sensors
US7381317B2 (en) * 2002-08-12 2008-06-03 Beckman Coulter, Inc. Methods and compositions for capillary electrophoresis (CE)
US7162372B2 (en) * 2002-10-08 2007-01-09 Tse-Wei Wang Least-square deconvolution (LSD): a method to resolve DNA mixtures
US6833919B2 (en) * 2002-10-11 2004-12-21 Combisep Multiplexed, absorbance-based capillary electrophoresis system and method
US7179357B2 (en) * 2002-10-25 2007-02-20 Beckman Coulter, Inc. Serial sample injection in capillary electrophoresis
US7108775B2 (en) * 2002-11-08 2006-09-19 Applera Corporation Apparatus and method for confining eluted samples in electrophoresis systems
US7402398B2 (en) 2003-07-17 2008-07-22 Monogram Biosciences, Inc. Measuring receptor homodimerization
WO2005019470A2 (en) 2003-08-11 2005-03-03 Monogram Biosciences, Inc. Detecting and profiling molecular complexes
US20050106596A1 (en) * 2003-08-27 2005-05-19 Third Wave Technologies, Inc. Nucleic acid analysis methods conducted in small reaction volumes
US7700287B2 (en) 2005-01-28 2010-04-20 Life Technologies Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target DNA template
JP2008535518A (ja) 2005-04-14 2008-09-04 アプレラ コーポレイション プソイドイソシトシン核酸塩基誘導体を含む３’改変オリゴヌクレオチド、およびプライマーまたはプローブとしてのその適用
US20070014699A1 (en) 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
JP5130634B2 (ja) * 2006-03-08 2013-01-30 ソニー株式会社 自発光表示装置、電子機器、焼き付き補正装置及びプログラム
US7664719B2 (en) * 2006-11-16 2010-02-16 University Of Tennessee Research Foundation Interaction method with an expert system that utilizes stutter peak rule
US7640223B2 (en) * 2006-11-16 2009-12-29 University Of Tennessee Research Foundation Method of organizing and presenting data in a table using stutter peak rule
US7624087B2 (en) * 2006-11-16 2009-11-24 University Of Tennessee Research Foundation Method of expert system analysis of DNA electrophoresis data
US20080145898A1 (en) * 2006-12-14 2008-06-19 Applera Corporation Sequencing methods
US9068977B2 (en) * 2007-03-09 2015-06-30 The Regents Of The University Of Michigan Non-linear rotation rates of remotely driven particles and uses thereof
US8235903B2 (en) * 2007-10-12 2012-08-07 Innoscion, Llc Remotely controlled implantable transducer and associated displays and controls
WO2010011944A2 (en) 2008-07-25 2010-01-28 Wagner Richard W Protein screeing methods
WO2012027747A2 (en) 2010-08-27 2012-03-01 The Regents Of The University Of Michigan Asynchronous magnetic bead rotation sensing systems and methods
US9816993B2 (en) 2011-04-11 2017-11-14 The Regents Of The University Of Michigan Magnetically induced microspinning for super-detection and super-characterization of biomarkers and live cells
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
US9797817B2 (en) 2012-05-03 2017-10-24 The Regents Of The University Of Michigan Multi-mode separation for target detection
US20160002624A1 (en) 2012-05-17 2016-01-07 Isis Pharmaceuticals, Inc. Antisense oligonucleotide compositions
US9574193B2 (en) 2012-05-17 2017-02-21 Ionis Pharmaceuticals, Inc. Methods and compositions for modulating apolipoprotein (a) expression
US9983110B2 (en) 2013-11-04 2018-05-29 The Regents Of The University Of Michigan Asynchronous magnetic bead rotation (AMBR) microviscometer for analysis of analytes
US10344326B2 (en) 2016-05-25 2019-07-09 International Business Machines Corporation Magnetic flux density based DNA sequencing
EP3475446A1 (de) 2016-06-27 2019-05-01 Juno Therapeutics, Inc. Verfahren zur identifizierung von peptidepitopen, moleküle zur bindung derartiger epitope und verwendungen davon
MA45491A (fr) 2016-06-27 2019-05-01 Juno Therapeutics Inc Épitopes à restriction cmh-e, molécules de liaison et procédés et utilisations associés
WO2019089982A1 (en) 2017-11-01 2019-05-09 Juno Therapeutics, Inc. Method of assessing activity of recombinant antigen receptors

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5089111A (en) * 1989-01-27 1992-02-18 Bio-Rad Laboratories, Inc. Electrophoretic sieving in gel-free media with dissolved polymers
US5332481A (en) * 1991-01-29 1994-07-26 Beckman Instruments, Inc. Capillary electrophoresis using replaceable gels
US5126021A (en) * 1991-07-17 1992-06-30 Applied Biosystems Inc. Low-viscosity polymer solution for capillary electrophoresis

Also Published As

Publication number Publication date
JP2002176976A (ja) 2002-06-25
CA2153991A1 (en) 1994-08-04
AU674390B2 (en) 1996-12-19
WO1994017207A1 (en) 1994-08-04
DE69434542T2 (de) 2006-08-10
AU6087094A (en) 1994-08-15
ATE310102T1 (de) 2005-12-15
JP2004000227A (ja) 2004-01-08
EP0680516B1 (de) 2005-11-16
CA2153991C (en) 2001-09-04
US5374527A (en) 1994-12-20
JPH08509596A (ja) 1996-10-15
EP0680516A1 (de) 1995-11-08

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Legal Events

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Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

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