DE69333672D1 - Rekombinante lipase und alpha-amylase varianten - Google Patents

Rekombinante lipase und alpha-amylase varianten

Info

Publication number
DE69333672D1
DE69333672D1 DE69333672T DE69333672T DE69333672D1 DE 69333672 D1 DE69333672 D1 DE 69333672D1 DE 69333672 T DE69333672 T DE 69333672T DE 69333672 T DE69333672 T DE 69333672T DE 69333672 D1 DE69333672 D1 DE 69333672D1
Authority
DE
Germany
Prior art keywords
alpha
pct
lipase
amylase
variants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69333672T
Other languages
English (en)
Other versions
DE69333672T2 (de
Inventor
Egon Nielsen
Grethe Rasmussen
Torben Halkier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of DE69333672D1 publication Critical patent/DE69333672D1/de
Publication of DE69333672T2 publication Critical patent/DE69333672T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE69333672T 1992-12-23 1993-12-22 Rekombinante lipase und alpha-amylase varianten Expired - Lifetime DE69333672T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK154292 1992-12-23
DK921542A DK154292D0 (da) 1992-12-23 1992-12-23 Nyt enzym
PCT/DK1993/000441 WO1994014951A1 (en) 1992-12-23 1993-12-22 Recombinant lipase and alpha-amylase variants

Publications (2)

Publication Number Publication Date
DE69333672D1 true DE69333672D1 (de) 2004-11-25
DE69333672T2 DE69333672T2 (de) 2006-03-09

Family

ID=8105895

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69333672T Expired - Lifetime DE69333672T2 (de) 1992-12-23 1993-12-22 Rekombinante lipase und alpha-amylase varianten

Country Status (10)

Country Link
US (1) US5731280A (de)
EP (1) EP0675949B1 (de)
JP (1) JPH08504586A (de)
KR (1) KR100296853B1 (de)
AT (1) ATE280220T1 (de)
BR (1) BR9307718A (de)
DE (1) DE69333672T2 (de)
DK (1) DK154292D0 (de)
FI (1) FI118969B (de)
WO (1) WO1994014951A1 (de)

Families Citing this family (52)

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DE69405735T3 (de) † 1993-05-26 2006-05-18 Unilever N.V. Waschmittelzusammensetzungen
US5830837A (en) * 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
BR9407767A (pt) * 1993-10-08 1997-03-18 Novo Nordisk As Variante de enzima &-amilase uso da mesma construção de DNA vetor de express o recombinante célula processos para produzir uma &-amilase hibrida e para preparar uma variante de uma &-amilase aditivo detergente e composições detergentes
TW255887B (en) * 1994-05-25 1995-09-01 Lilly Co Eli Synthesis of benzoquinolinones
GB2296011B (en) * 1994-12-13 1999-06-16 Solvay Novel fusarium isolate and lipases, cutinases and enzyme compositions derived therefrom
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
EP0756001A1 (de) * 1995-07-24 1997-01-29 The Procter & Gamble Company Waschmittelzusammensetzungen enthaltend eine spezifische Amylase une ein spezifisches oberflächenaktives System
US5741767A (en) * 1995-11-16 1998-04-21 Lever Brothers Company, Division Of Conopco, Inc. Peracid based dishwashing detergent composition
US6077818A (en) * 1996-02-20 2000-06-20 The Procter & Gamble Company Cellulase activity control by a terminator
WO1997041213A1 (en) 1996-04-30 1997-11-06 Novo Nordisk A/S α-AMYLASE MUTANTS
BR9609712A (pt) * 1996-06-19 1999-02-23 Procter & Gamble Composições detergentes compreendendo uma amilase específica e uma protease
US6140475A (en) * 1997-04-11 2000-10-31 Altus Biologics Inc. Controlled dissolution crosslinked protein crystals
ATE231186T1 (de) 1998-07-21 2003-02-15 Danisco Lebensmittel
US6887986B1 (en) * 1998-11-16 2005-05-03 Novozymes A/S α-amylase variants
US6080687A (en) * 1999-03-18 2000-06-27 Zydex Industries Method of dyeing anionic materials with pigment colors having a net cationic charge using a padding process
CA2383228A1 (en) 1999-09-22 2001-03-29 The Procter & Gamble Company A hand-held liquid container
AU1269601A (en) * 1999-11-10 2001-06-06 Novozymes A/S Fungamyl-like alpha-amylase variants
US7005288B1 (en) * 1999-11-10 2006-02-28 Novozymes A/S Fungamyl-like alpha-amylase variants
US20010046493A1 (en) * 2000-02-24 2001-11-29 Alex Margolin Lipase-containing composition and methods of use thereof
DE50107849D1 (de) * 2000-07-28 2005-12-01 Henkel Kgaa Neues amylolytisches enzym aus bacillus sp. a 7-7 (dsm 12368) sowie wasch- und reinigungsmittel mit diesem neuen amylolytischen enzym
JP2005502311A (ja) * 2001-01-24 2005-01-27 レキシコン・ジェネティクス・インコーポレーテッド 新規ヒトリパーゼ及び該リパーゼをコードするポリヌクレオチド
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
US7153820B2 (en) 2001-08-13 2006-12-26 Ecolab Inc. Solid detergent composition and method for solidifying a detergent composition
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
MXPA05007653A (es) 2003-01-17 2005-09-30 Danisco Metodo.
US7142464B2 (en) * 2003-04-29 2006-11-28 Saifun Semiconductors Ltd. Apparatus and methods for multi-level sensing in a memory array
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
KR20120076377A (ko) 2004-01-16 2012-07-09 다우 글로벌 테크놀로지스 엘엘씨 슈도모나스 플루오레센스에서의 포유류 단백질의 발현
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
EP1791933B1 (de) 2004-07-16 2011-06-29 Danisco A/S Enzymatisches öldegummierungsverfahren
CN101031655A (zh) 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
PT2198880T (pt) * 2004-10-14 2017-01-04 Lilly Co Eli Composições contendo lipase, protease e amilase para o tratamento de insuficiência pancreática
EP1700904A1 (de) 2005-03-11 2006-09-13 Unilever N.V. Flüssige Waschmittelzusammensetzung
EP1700907A1 (de) 2005-03-11 2006-09-13 Unilever N.V. Flüssige bleichmittelzusammensetzung
US20100196344A1 (en) * 2005-10-14 2010-08-05 Cystic Fibrosis Foundation Therapeutics, Inc. Compositions and methods for treating pancreatic insufficiency
BRPI0720801A2 (pt) 2007-01-25 2014-09-16 Dupont Nutrition Biosci Aps Produção de um lipídio aciltranfersase a partir de células transformadas de bacillus licheniformis.
US8093200B2 (en) 2007-02-15 2012-01-10 Ecolab Usa Inc. Fast dissolving solid detergent
WO2008134461A2 (en) 2007-04-27 2008-11-06 Dow Global Technologies, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
ES2439958T3 (es) 2007-10-18 2014-01-27 Ecolab Inc. Composiciones de limpieza sólidas, céreas y comprimidas, y métodos de hacerlas
US8853269B2 (en) * 2010-02-04 2014-10-07 Copperhead Chemical Company Inc. Composition and method for treating infections and promoting intestinal health
ES2752079T3 (es) 2011-05-20 2020-04-02 Ecolab Usa Inc Formulaciones ácidas para uso en un sistema de lavado de utensilios
EP2792737A1 (de) 2011-05-20 2014-10-22 Ecolab USA Inc. Phosphatfreie Reinigungsmittel und phosphatfreie Säuren in einem alternierenden Alkali-/Säuresystem zur Reinigung von Artikeln
ES2750872T3 (es) 2011-12-13 2020-03-27 Ecolab Usa Inc Método de lavado de vajilla
WO2014007921A1 (en) 2012-06-08 2014-01-09 Danisco Us Inc. Variant alpha amylases with enhanced activity on starch polymers
US9745543B2 (en) 2012-09-10 2017-08-29 Ecolab Usa Inc. Stable liquid manual dishwashing compositions containing enzymes
US20160017305A1 (en) 2013-03-11 2016-01-21 Danisco Us Inc. Alpha-amylase combinatorial variants
WO2021022045A1 (en) 2019-07-31 2021-02-04 Ecolab Usa Inc. Personal protective equipment free delimer compositions

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61104784A (ja) * 1984-10-26 1986-05-23 Suntory Ltd ペルオキシダ−ゼの製造法
US5030240A (en) * 1986-06-09 1991-07-09 The Clorox Company Enzymatic peracid bleaching system
DE3750450T2 (de) * 1986-08-29 1995-01-05 Novo Industri As Enzymhaltiger Reinigungsmittelzusatz.
US5108457A (en) * 1986-11-19 1992-04-28 The Clorox Company Enzymatic peracid bleaching system with modified enzyme
EP0305216B1 (de) * 1987-08-28 1995-08-02 Novo Nordisk A/S Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen
DK212388D0 (da) * 1988-04-15 1988-04-15 Novo Industri As Detergent additiv
BR9006818A (pt) * 1989-06-29 1991-08-06 Gist Brocades Nv Amilases microbianas mutantes com maior estabilidade termica,acida e/ou alcalina
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
PE14291A1 (es) * 1989-10-13 1991-04-27 Novo Nordisk As Procedimiento para inhibir la transferencia de tintes
GB9027836D0 (en) * 1990-12-21 1991-02-13 Unilever Plc Enzymes and enzymatic detergent compositions
DK0580707T3 (da) * 1991-04-12 1998-01-19 Novo Nordisk As Fjernelse af overskydende farvestof fra nyligt fremstillede tekstiler
WO1992018683A1 (en) * 1991-04-12 1992-10-29 Novo Nordisk A/S Process for bleaching of dyed textiles
EP0667915A1 (de) * 1991-11-26 1995-08-23 Novo Nordisk A/S Proteasefeste proteine

Also Published As

Publication number Publication date
FI953128A (fi) 1995-06-22
WO1994014951A1 (en) 1994-07-07
KR100296853B1 (ko) 2001-11-26
JPH08504586A (ja) 1996-05-21
EP0675949B1 (de) 2004-10-20
FI953128A0 (fi) 1995-06-22
US5731280A (en) 1998-03-24
FI118969B (fi) 2008-05-30
DE69333672T2 (de) 2006-03-09
BR9307718A (pt) 1999-09-08
ATE280220T1 (de) 2004-11-15
KR960700339A (ko) 1996-01-19
DK154292D0 (da) 1992-12-23
EP0675949A1 (de) 1995-10-11

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Legal Events

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