DE69331908D1 - Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen - Google Patents

Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen

Info

Publication number
DE69331908D1
DE69331908D1 DE69331908T DE69331908T DE69331908D1 DE 69331908 D1 DE69331908 D1 DE 69331908D1 DE 69331908 T DE69331908 T DE 69331908T DE 69331908 T DE69331908 T DE 69331908T DE 69331908 D1 DE69331908 D1 DE 69331908D1
Authority
DE
Germany
Prior art keywords
expression
plant cells
foreign genes
transcription initiation
initiation region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69331908T
Other languages
English (en)
Other versions
DE69331908T2 (de
Inventor
F Thomson
L Spiker
C Allen
E Hall
C Childs
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
North Carolina State University
Original Assignee
North Carolina State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by North Carolina State University filed Critical North Carolina State University
Publication of DE69331908D1 publication Critical patent/DE69331908D1/de
Application granted granted Critical
Publication of DE69331908T2 publication Critical patent/DE69331908T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
DE69331908T 1992-10-05 1993-09-27 Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen Expired - Lifetime DE69331908T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95642092A 1992-10-05 1992-10-05
PCT/US1993/009179 WO1994007902A1 (en) 1992-10-05 1993-09-27 Method for increasing expression and reducing expression variability of foreign genes in plant cells

Publications (2)

Publication Number Publication Date
DE69331908D1 true DE69331908D1 (de) 2002-06-13
DE69331908T2 DE69331908T2 (de) 2002-12-12

Family

ID=25498228

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69331908T Expired - Lifetime DE69331908T2 (de) 1992-10-05 1993-09-27 Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen

Country Status (10)

Country Link
US (1) US5773689A (de)
EP (1) EP0663921B1 (de)
AT (1) ATE217319T1 (de)
AU (1) AU673859B2 (de)
CA (1) CA2147006C (de)
DE (1) DE69331908T2 (de)
DK (1) DK0663921T3 (de)
ES (1) ES2176208T3 (de)
PT (1) PT663921E (de)
WO (1) WO1994007902A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6239328B1 (en) 1992-10-05 2001-05-29 North Carolina State University Method for reducing expression variability of transgenes in plant cells
US6040185A (en) * 1993-04-19 2000-03-21 Novartis Ag Genetic stabilizing elements
AU6679094A (en) * 1993-04-19 1994-11-08 Sandoz Ltd. Genetic stabilizing elements
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US6037525A (en) * 1996-08-01 2000-03-14 North Carolina State University Method for reducing expression variability of transgenes in plant cells
WO1998016650A1 (en) * 1996-10-17 1998-04-23 E.I. Du Pont De Nemours And Company Enhanced transgene expression in a population of monocot cells employing scaffold attachment regions
BR9807899A (pt) * 1997-03-28 2000-02-22 Mycogen Plant Science Inc Processo de transformação de plantas por sar aperfeiçoado
CA2292842A1 (en) * 1997-06-03 1998-12-10 North Carolina State University Method for reducing expression variability of transgenes in plants cells
US6245974B1 (en) 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6518485B1 (en) * 1998-06-04 2003-02-11 Westvaco Corporation Particle-mediated conifer transformation
AU3476399A (en) * 1998-07-31 2000-02-21 Mycogen Plant Science, Inc. Improved plant transformation process by scaffold attachment regions (sar)
AU762382B2 (en) * 1998-09-29 2003-06-26 Pioneer Hi-Bred International, Inc. MAR/SAR elements flanking RSYN7-driven construct
US6482646B1 (en) 1998-11-06 2002-11-19 E. I. Du Pont De Nemours And Company Plant proteins that interact with nuclear matrix proteins and function as transcriptional activators
WO2000032800A1 (en) * 1998-12-01 2000-06-08 Dow Agrosciences Llc Artificial matrix attachment region for increasing expression of genes introduced in plant cells
US6395962B1 (en) 1999-06-22 2002-05-28 University Of South Carolina Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons
US7126041B1 (en) 1999-12-10 2006-10-24 North Carolina State Unversity High efficiency gene targeting in plants
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
WO2002061039A2 (en) * 2000-10-25 2002-08-08 Washington State University Research Foundation Thuja plicata dirigent protein promotors
ES2330201T3 (es) 2001-01-26 2009-12-07 Selexis S.A. Regiones de union a la matriz y metodos para el uso de las mismas.
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
EP1279737A1 (de) 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformationsmethode zur Erzeugung von Pflanzen ohne Selektionsmarker
US20040016014A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US20040016015A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
EP1675454A4 (de) * 2003-08-19 2007-03-21 22Nd Century Ltd Llc Tabakprodukte mit verminderter exposition
EP1959011B1 (de) 2003-10-24 2012-03-28 Selexis S.A. Gentransfer und Expression mit hoher Effizienz in Säugerzellen mittels eines Mehrfachtransfektionsverfahrens von Matrixbindungsbereichsequenzen
US7553666B2 (en) 2005-11-04 2009-06-30 Dow Agrosciences Llc Preparation of vaccine master cell lines using recombinant plant suspension cultures
BRPI0721141A2 (pt) 2006-12-22 2014-04-01 Dow Agroscience Llc Vacinasdo vírus west nile (wnv) produzidas por plantas, vetores e sequências otimizadas de códons de plantas.
PL2655629T3 (pl) * 2010-12-24 2024-10-28 BASF Agricultural Solutions Seed US LLC Roślina brassica zawierająca zmutowany allel alcatraz
US20150052636A1 (en) * 2011-09-15 2015-02-19 Basf Plant Science Company Gmbh Regulatory Nucleic Acid Molecules for Reliable Gene Expression in Plants
EP3782465B1 (de) 2019-08-23 2023-08-30 Universität Potsdam Transgenes zebrafischmodell für schlaganfall

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5122466A (en) * 1989-06-13 1992-06-16 North Carolina State University Ballistic transformation of conifers
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
EP0519011A1 (de) * 1990-03-05 1992-12-23 The Upjohn Company Protein-expression durch verwendung von samenspezifischen regulierungssequenzen
US5187267A (en) * 1990-06-19 1993-02-16 Calgene, Inc. Plant proteins, promoters, coding sequences and use
MX9200621A (es) * 1991-02-14 1993-02-01 Du Pont Gen de una proteina con alto contenido de azufre de una semilla y metodo para aumentar el contenido de azufre en aminoacidos de las plantas.
WO1993019190A1 (en) * 1992-03-19 1993-09-30 E.I. Du Pont De Nemours And Company Nucleic acid fragments and methods for increasing the lysine and threonine content of the seeds of plants
AU6679094A (en) * 1993-04-19 1994-11-08 Sandoz Ltd. Genetic stabilizing elements

Also Published As

Publication number Publication date
WO1994007902A1 (en) 1994-04-14
PT663921E (pt) 2002-10-31
AU673859B2 (en) 1996-11-28
EP0663921A4 (de) 1996-07-17
CA2147006C (en) 2004-11-30
ES2176208T3 (es) 2002-12-01
EP0663921A1 (de) 1995-07-26
CA2147006A1 (en) 1994-04-14
ATE217319T1 (de) 2002-05-15
AU5165593A (en) 1994-04-26
EP0663921B1 (de) 2002-05-08
DK0663921T3 (da) 2002-09-02
DE69331908T2 (de) 2002-12-12
US5773689A (en) 1998-06-30

Similar Documents

Publication Publication Date Title
DE69331908D1 (de) Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen
NZ333998A (en) Method for reducing expression variability of transgenes in plant cells using DNA constructs comprising insulator elements
ATE348891T1 (de) Wurzelrinden spezifischer genpromoter
ATE41784T1 (de) Pflanzengen-expression.
ATE64416T1 (de) Die verwendung des gal 1 hefe-promotors.
DE3851399D1 (de) Transientes Expressionssystem zur Herstellung von rekombinantem Protein.
ES2180641T3 (es) Inhibicion citoplasmica de la expresion genetica.
DE69332763D1 (de) Virale amplifikation rekombinanter boten-rna in transgenen pflanzen
ATE52800T1 (de) Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren.
DE69834908D1 (de) Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen
ATE213020T1 (de) Klonierung und expression von biologisch aktiver alpha-galaktosidase a
ATE34770T1 (de) Dna-vektoren und ihre verwendung in der rekombinanten dna-technologie.
DE69328994D1 (de) Verfahren zur Herstellung eines fremden Gens oder dessen Produkt in Pflanzenzellen
CA2037677A1 (en) Process for production of exogenous gene or its product in plant cells
ATE162852T1 (de) Biosynthetisches verfahren zur herstellung von protein
ATE173762T1 (de) Klonierung der n-methylhydantoinase
CA2244204A1 (en) A plant nuclear scaffold attachment region which increases gene expression
DE58909205D1 (de) Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten.
KR970001543A (ko) 신규 프로모터 및 이를 이용한 유전자 발현 방법
Maubon Criteria for Selecting an Oxygen Process
MX9701598A (es) Un promotor de plantas util para dirigir la expresion de proteinas extrañas a la epidermis de la planta.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition