DE69326680T2 - Verfahren zum aufbringen/zurückgewinnen einer gelelektrophorese-probe - Google Patents

Verfahren zum aufbringen/zurückgewinnen einer gelelektrophorese-probe

Info

Publication number
DE69326680T2
DE69326680T2 DE69326680T DE69326680T DE69326680T2 DE 69326680 T2 DE69326680 T2 DE 69326680T2 DE 69326680 T DE69326680 T DE 69326680T DE 69326680 T DE69326680 T DE 69326680T DE 69326680 T2 DE69326680 T2 DE 69326680T2
Authority
DE
Germany
Prior art keywords
gelelectrophorese
recovering
sample
applying
gelelectrophorese sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69326680T
Other languages
English (en)
Other versions
DE69326680D1 (de
Inventor
Erich Gombocz
Mark Dwight
David Rammler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Labintelligence Inc
Original Assignee
Labintelligence Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Labintelligence Inc filed Critical Labintelligence Inc
Publication of DE69326680D1 publication Critical patent/DE69326680D1/de
Application granted granted Critical
Publication of DE69326680T2 publication Critical patent/DE69326680T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE69326680T 1992-09-30 1993-08-26 Verfahren zum aufbringen/zurückgewinnen einer gelelektrophorese-probe Expired - Fee Related DE69326680T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/954,911 US5217591A (en) 1990-05-14 1992-09-30 Gel electrophoresis sample applicator/retriever
PCT/US1993/008012 WO1994008234A1 (en) 1992-09-30 1993-08-26 Gel electrophoresis sample applicator/retriever

Publications (2)

Publication Number Publication Date
DE69326680D1 DE69326680D1 (de) 1999-11-11
DE69326680T2 true DE69326680T2 (de) 2000-03-02

Family

ID=25496100

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69326680T Expired - Fee Related DE69326680T2 (de) 1992-09-30 1993-08-26 Verfahren zum aufbringen/zurückgewinnen einer gelelektrophorese-probe

Country Status (5)

Country Link
US (1) US5217591A (de)
EP (1) EP0614528B1 (de)
CA (1) CA2124345C (de)
DE (1) DE69326680T2 (de)
WO (1) WO1994008234A1 (de)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
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JPH05236997A (ja) * 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
US5384022A (en) * 1993-09-08 1995-01-24 Cornell Research Foundation, Inc. Method and apparatus for electrophoretic DNA band isolation
IL112147A (en) * 1994-01-19 1999-12-22 Du Pont Sample holder and method for automated electrophoresis
US5538614A (en) * 1994-08-17 1996-07-23 Han; Dawn D. Macromolecule recovery cassette
US5627022A (en) * 1994-11-01 1997-05-06 Visible Genetics Inc. Microgels for use in medical diagnosis and holders useful in fabricating same
JP3434914B2 (ja) * 1994-11-11 2003-08-11 株式会社日立製作所 電気泳動装置用試料保持装置、電気泳動装置及び電気泳動装置への試料注入方法
US5972188A (en) * 1995-03-03 1999-10-26 Genetic Biosystems, Inc. Membrane loader for gel electrophoresis
US7824532B2 (en) 1995-04-26 2010-11-02 Life Technologies Corporation Apparatus and method for electrophoresis
US5587062A (en) * 1996-01-24 1996-12-24 Shimadzu Corporation Sample collecting apparatus by gel electrophoresis
DE69726459T2 (de) * 1996-05-06 2004-05-27 Helena Laboratories Corp., Beaumont Vorrichtung zum auftragen von proben auf ein substrat
US5759375A (en) * 1996-05-17 1998-06-02 Purdue Research Foundation Miniaturized disposable gels for DNA analysis
US5656145A (en) * 1996-05-20 1997-08-12 Bio-Rad Laboratories, Inc. Needle guide for loading samples into a vertical slab gel
EP0927268A1 (de) * 1996-08-27 1999-07-07 Visible Genetics Inc. Vorrichtung und verfahren zur sequenzierung von nukleinsäuren
GB9624927D0 (en) 1996-11-29 1997-01-15 Oxford Glycosciences Uk Ltd Gels and their use
US6554991B1 (en) 1997-06-24 2003-04-29 Large Scale Proteomics Corporation Automated system for two-dimensional electrophoresis
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
DE19815400C2 (de) * 1998-04-06 2002-02-07 Max Planck Gesellschaft Vorrichtung zur Probenaufnahme aus polymeren Trägermaterialien
GB9811656D0 (en) 1998-05-29 1998-07-29 Oxford Glycosciences Uk Ltd Gels, methods and apparatus for identification and characterization of biomolecules
US6024854A (en) * 1998-06-11 2000-02-15 Visible Genetics, Inc. Method and apparatus for improving electrophoresis resolution
US6402914B1 (en) * 1998-08-14 2002-06-11 The Regents Of The University Of California In-situ loader for electrophoretic gels
US6132582A (en) 1998-09-14 2000-10-17 The Perkin-Elmer Corporation Sample handling system for a multi-channel capillary electrophoresis device
US6406602B1 (en) 1998-12-23 2002-06-18 Genome Therapeutics Corporation Sample loading device for gel electrophoresis
US7572788B2 (en) 1999-04-30 2009-08-11 The Regents Of The University Of Michigan Compositions and methods relating to novel compounds and targets thereof
US20060025388A1 (en) 1999-04-30 2006-02-02 Glick Gary D Compositions and methods relating to novel compounds and targets thereof
US6342143B1 (en) 2000-01-06 2002-01-29 Carnegie Mellon University Cutting tool for multiple sample retrieval from gelatinous material
US6495017B1 (en) * 2000-04-10 2002-12-17 Amersham Pharmacia Biotech, Inc. Sample loading means for electrophoresis gel strips
US6565728B1 (en) * 2000-06-08 2003-05-20 Elchrom Scientific Gel cutting and recovering device
US6562213B1 (en) * 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
US6582577B1 (en) 2000-08-31 2003-06-24 Visible Genetics Inc. Electrophoresis gel cassette
US7070682B2 (en) * 2001-01-16 2006-07-04 Cheng Lee Microfluidic apparatus for performing gel protein extractions and methods for using the apparatus
US7641780B2 (en) 2001-05-01 2010-01-05 Calibrant Biosystems, Inc. Two-dimensional microfluidics for protein separations and gene analysis
US6974526B2 (en) * 2001-05-01 2005-12-13 Calibrant Biosystems, Inc. Plastic microfluidics enabling two-dimensional protein separations in proteome analysis
US6929730B2 (en) 2001-05-01 2005-08-16 Cheng Sheng Lee Two dimensional microfluidic gene scanner
US7033477B2 (en) * 2001-11-30 2006-04-25 Grace Ventures Llc Electrophoresis gel assembly
US6702990B1 (en) * 2002-02-05 2004-03-09 The Gel Company Spot picker
ITMI20020367A1 (it) 2002-02-25 2003-08-25 Pietro Nardo Applicatore di un campione di fluido ad un substrato
US7247275B2 (en) * 2004-06-21 2007-07-24 Jeremy Scot Caldwell Gel extraction device
AU2006203946B2 (en) 2005-01-03 2009-07-23 The Regents Of The University Of Michigan Compositions and methods relating to novel compounds and targets thereof
EP1917522A1 (de) * 2005-06-18 2008-05-07 GE Healthcare Bio-Sciences AB Verfahren und vorrichtungen zur bildung mehrerer vertiefungen auf einem gel
AU2006261309A1 (en) * 2005-06-18 2006-12-28 Ge Healthcare Bio-Sciences Ab Methods and systems for adding a reagent to an analyte in a gel
US8163153B2 (en) 2005-12-20 2012-04-24 Caldwell Jeremy S Tool for extracting electrophoretic sample
US20080160629A1 (en) * 2007-01-02 2008-07-03 Calibrant Biosystems, Inc. Methods and systems for off-line multidimensional concentration and separation of biomolecules
US20080156080A1 (en) * 2007-01-02 2008-07-03 Calibrant Biosystems, Inc. Methods and systems for multidimensional concentration and separation of biomolecules using capillary isotachophoresis
US8361299B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
AU2009302248B2 (en) * 2008-10-08 2014-03-06 Sage Science, Inc. Multichannel preparative electrophoresis system
WO2011062765A2 (en) 2009-11-17 2011-05-26 The Regents Of The University Of Michigan 1,4-benzodiazepine-2,5-diones and related compounds with therapeutic properties
DE102009057223B4 (de) * 2009-12-05 2016-03-24 Chemagen Biopolymer-Technologie Aktiengesellschaft Probengefäßmatrix und deren Herstellungsverfahren
AU2013329110B2 (en) 2012-10-12 2017-05-04 Sage Science, Inc. Side-eluting molecular fractionator
EP3207163B1 (de) 2014-10-15 2020-05-27 Sage Science, Inc. Vorrichtungen, verfahren und systeme zur automatisierten verarbeitung von nukleinsäuren und elektrophoretischen probenvorbereitung
CN105259241B (zh) * 2015-11-02 2018-01-30 中国人民解放军第四军医大学 一种用于蛋白跑胶的多v形管上样器
JP7054678B2 (ja) 2015-11-20 2022-04-14 ワシントン・ユニバーシティ ゲノムｄｎａ断片の標的化された精製のための調製用電気泳動方法
US11867661B2 (en) 2017-04-07 2024-01-09 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification
CN111812091B (zh) * 2020-06-28 2023-09-05 上海交通大学 芯片凝胶电泳及其在线uv-vis成像检测装置
CN114277027A (zh) * 2021-12-23 2022-04-05 深圳大学 一种用于电泳分离物回收的用具及其使用方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4431506A (en) * 1982-09-07 1984-02-14 E-C Apparatus Corporation Apparatus for gel electrophoresis
US5139637A (en) * 1991-03-13 1992-08-18 Macconnell William P Plasmid purification system and method

Also Published As

Publication number Publication date
EP0614528B1 (de) 1999-10-06
CA2124345A1 (en) 1994-04-14
CA2124345C (en) 2003-12-16
DE69326680D1 (de) 1999-11-11
WO1994008234A1 (en) 1994-04-14
EP0614528A1 (de) 1994-09-14
EP0614528A4 (de) 1994-10-26
US5217591A (en) 1993-06-08

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee