DE69324375D1 - Herstellungsprozess von fremdprotein - Google Patents

Herstellungsprozess von fremdprotein

Info

Publication number
DE69324375D1
DE69324375D1 DE69324375T DE69324375T DE69324375D1 DE 69324375 D1 DE69324375 D1 DE 69324375D1 DE 69324375 T DE69324375 T DE 69324375T DE 69324375 T DE69324375 T DE 69324375T DE 69324375 D1 DE69324375 D1 DE 69324375D1
Authority
DE
Germany
Prior art keywords
foreign protein
cell
manufacturing process
serum
free medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69324375T
Other languages
English (en)
Other versions
DE69324375T2 (de
Inventor
Kiyoto Nishiyama
Yuji Ishikawa
Kazuhiko Kimachi
Hiroaki Maeda
Sachio Tokiyoshi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemo Sero Therapeutic Research Institute Kaketsuken
Original Assignee
Chemo Sero Therapeutic Research Institute Kaketsuken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemo Sero Therapeutic Research Institute Kaketsuken filed Critical Chemo Sero Therapeutic Research Institute Kaketsuken
Application granted granted Critical
Publication of DE69324375D1 publication Critical patent/DE69324375D1/de
Publication of DE69324375T2 publication Critical patent/DE69324375T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE69324375T 1992-11-28 1993-11-25 Herstellungsprozess von fremdprotein Expired - Fee Related DE69324375T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP34125692 1992-11-28
PCT/JP1993/001723 WO1994012658A1 (en) 1992-11-28 1993-11-25 Process for producing foreign protein

Publications (2)

Publication Number Publication Date
DE69324375D1 true DE69324375D1 (de) 1999-05-12
DE69324375T2 DE69324375T2 (de) 1999-08-26

Family

ID=18344632

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69324375T Expired - Fee Related DE69324375T2 (de) 1992-11-28 1993-11-25 Herstellungsprozess von fremdprotein

Country Status (9)

Country Link
US (1) US5683891A (de)
EP (1) EP0671471B1 (de)
JP (1) JP3415840B2 (de)
AT (1) ATE178655T1 (de)
AU (1) AU666800B2 (de)
CA (1) CA2150344C (de)
DE (1) DE69324375T2 (de)
DK (1) DK0671471T3 (de)
WO (1) WO1994012658A1 (de)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757018A (en) * 1985-02-11 1988-07-12 Hazleton Biotechnologies, Inc. Myeloma cell lines and uses thereof
GB2183662B (en) * 1985-04-01 1989-01-25 Celltech Ltd Transformed myeloma cell-line and a process for the expression of a gene coding for a eukaryotic polypeptide employing same
CA1339198C (en) * 1988-02-12 1997-08-05 Gregory Paul Winter Antibodies to the antigen campath-1
JPH0257184A (ja) * 1988-08-23 1990-02-26 Fujirebio Inc Ｂ型肝炎ウイルスコアタンパク質発現ベクター及びそれを含むミエローマ細胞
GB8909218D0 (en) * 1989-04-22 1989-06-07 Medical Res Council Improvements in or relating to enhancers
IL94872A (en) * 1989-06-30 1995-03-30 Oncogen Monoclonal or chimeric antibodies that react with human carcinoma, recombinant proteins that contain their anti-binding site, pharmaceutical preparations and kits that contain the
US5110737A (en) * 1989-07-31 1992-05-05 W. Alton Jones Cell Science Center Incorporated Method for the isolation of hybridomas using cholesterol auxotrophy of myeloma cells
JP3043023B2 (ja) * 1989-12-12 2000-05-22 森永製菓株式会社 ハイブリドーマ
JPH09301723A (ja) * 1996-05-17 1997-11-25 Asahi Glass Co Ltd プランジャー支持機構および支持方法

Also Published As

Publication number Publication date
CA2150344C (en) 2001-02-13
DE69324375T2 (de) 1999-08-26
WO1994012658A1 (en) 1994-06-09
DK0671471T3 (da) 1999-10-18
AU5596094A (en) 1994-06-22
CA2150344A1 (en) 1994-06-09
EP0671471B1 (de) 1999-04-07
EP0671471A4 (de) 1996-09-11
EP0671471A1 (de) 1995-09-13
US5683891A (en) 1997-11-04
JP3415840B2 (ja) 2003-06-09
AU666800B2 (en) 1996-02-22
ATE178655T1 (de) 1999-04-15

Similar Documents

Publication Publication Date Title
EP0343783A3 (en) Method for producing cells containing stably integrated foreign dna at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign dna
PT88479A (pt) Metodo para cultura de celulas recombinantes
DE3888561D1 (de) Hohe Proteinsyntheserate in Hefe.
IL68366A (en) Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture
EP0537173A4 (en) Cloning by complementation and related processes
DK0700995T3 (da) Fremgangsmåde til fremstilling af protein
TR200101642T2 (tr) Memelilerde gen sentezlemesi için insan hibrid konukçu hücresi
ATE91718T1 (de) Rekombinante dns-molekuele die hepatitis-boberfl|chenantigenkodieren, deren herstellung und abgeleitete vakzine.
DK359487D0 (da) Fremgangsmaade til udvirkning af foroeget ekspression af rekombinant faktor viii i en vaertscelle
DE69324375D1 (de) Herstellungsprozess von fremdprotein
NO944846D0 (no) Fremgangsmåte for fremstilling av protein C
ATE129287T1 (de) Bakterielles kollagenase-gen.
DE3878410D1 (de) Verfahren zur erhoehung von gen-expression.
NO994788D0 (no) FremgangsmÕte for fremstilling av heterolog protein
DK0485504T3 (da) Fremgangsmåde til fremstilling af aktiveret protein C ved celledyrkning
NZ515762A (en) Method of producing a heparin-binding protein by expressing it in a mammalian cell that can be cultured in anaerobic conditions
DE69202858D1 (de) Herstellungsverfahren für chymosin.
ATE288476T1 (de) Herstellung von proteinen
DK0646645T3 (da) Fremgangsmåde til fremstilling af et stof

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee