DE69316205T2 - Verfahren und reagens zur spaltung und entschützen von oligonukleotide - Google Patents

Verfahren und reagens zur spaltung und entschützen von oligonukleotide

Info

Publication number
DE69316205T2
DE69316205T2 DE69316205T DE69316205T DE69316205T2 DE 69316205 T2 DE69316205 T2 DE 69316205T2 DE 69316205 T DE69316205 T DE 69316205T DE 69316205 T DE69316205 T DE 69316205T DE 69316205 T2 DE69316205 T2 DE 69316205T2
Authority
DE
Germany
Prior art keywords
cleaving
reagent
protecting oligonucleotides
oligonucleotides
protecting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69316205T
Other languages
English (en)
Other versions
DE69316205D1 (de
Inventor
Parameswara Reddy
Naeem Hanna
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Publication of DE69316205D1 publication Critical patent/DE69316205D1/de
Application granted granted Critical
Publication of DE69316205T2 publication Critical patent/DE69316205T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
DE69316205T 1992-04-24 1993-04-09 Verfahren und reagens zur spaltung und entschützen von oligonukleotide Expired - Lifetime DE69316205T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/873,915 US5348868A (en) 1992-04-24 1992-04-24 Methods and reagents for cleaving and deprotecting oligonucleotides
PCT/US1993/003386 WO1993022329A1 (en) 1992-04-24 1993-04-09 Methods and reagents for cleaving and deprotecting oligonucleotides

Publications (2)

Publication Number Publication Date
DE69316205D1 DE69316205D1 (de) 1998-02-12
DE69316205T2 true DE69316205T2 (de) 1998-05-07

Family

ID=25362594

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69316205T Expired - Lifetime DE69316205T2 (de) 1992-04-24 1993-04-09 Verfahren und reagens zur spaltung und entschützen von oligonukleotide

Country Status (5)

Country Link
US (2) US5348868A (de)
EP (1) EP0639200B1 (de)
JP (1) JP3368353B2 (de)
DE (1) DE69316205T2 (de)
WO (1) WO1993022329A1 (de)

Families Citing this family (41)

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Publication number Priority date Publication date Assignee Title
US5616700A (en) * 1992-04-24 1997-04-01 Beckman Instruments, Inc. Processes for synthesizing nucleotides and modified nucleotides using N.sub.
US6469158B1 (en) * 1992-05-14 2002-10-22 Ribozyme Pharmaceuticals, Incorporated Synthesis, deprotection, analysis and purification of RNA and ribozymes
US5686599A (en) * 1992-05-14 1997-11-11 Ribozyme Pharmaceuticals, Inc. Synthesis, deprotection, analysis and purification of RNA and ribozymes
US5977343A (en) 1992-05-14 1999-11-02 Ribozyme Pharmaceuticals, Inc. Synthesis, deprotection, analysis and purification of RNA and ribozymes
US5623068A (en) * 1994-03-07 1997-04-22 Beckman Instruments, Inc. Synthesis of DNA using substituted phenylacetyl-protected nucleotides
US5808045A (en) * 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6232465B1 (en) 1994-09-02 2001-05-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6214987B1 (en) * 1994-09-02 2001-04-10 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US5872244A (en) * 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5763594A (en) * 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5990300A (en) * 1994-09-02 1999-11-23 Andrew C. Hiatt Enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5681534A (en) * 1995-07-20 1997-10-28 Neves; Richard S. High throughput oligonucleotide synthesizer
US5731429A (en) * 1995-10-18 1998-03-24 Beckman Instruments, Inc. Cac -methylphosphonamidites and methods for preparing methylphosphonates
US5808039A (en) * 1995-10-18 1998-09-15 Beckman Instruments, Inc. 2'-OMe CAC phosphoramidite and methods for preparation and use thereof
CA2234159A1 (en) 1995-10-19 1997-04-24 Alecia Settle Method for solution phase synthesis of oligonucleotides
US5869696A (en) * 1996-04-22 1999-02-09 Beckman Instruments, Inc. Universal solid supports and methods for their use
US5750672A (en) * 1996-11-22 1998-05-12 Barrskogen, Inc. Anhydrous amine cleavage of oligonucleotides
US5770723A (en) * 1997-03-12 1998-06-23 Reddy; M. Paraweswara Processes for purifying synthetic oligonucleotides
US6326479B1 (en) 1998-01-27 2001-12-04 Boston Probes, Inc. Synthetic polymers and methods, kits or compositions for modulating the solubility of same
US6110630A (en) * 1998-06-18 2000-08-29 Beckman Coulter, Inc. Efficient activated cyanine dyes
AU2998800A (en) 1999-03-24 2000-10-09 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The N-acylphosphoramidites and their use in oligonucleotide synthesis
CA2375060A1 (en) 1999-05-24 2000-11-30 Invitrogen Corporation Method for deblocking of labeled oligonucleotides
US6147200A (en) * 1999-08-19 2000-11-14 Isis Pharmaceuticals, Inc. 2'-O-acetamido modified monomers and oligomers
EP1294736A1 (de) * 2000-06-12 2003-03-26 Avecia Biotechnology, Inc. Verfahren zum entschützen von oligonukleotiden mit einem acrylonitrilfänger
DE60138916D1 (de) 2000-09-26 2009-07-16 Boston Probes Inc Sonden, sondensätzen,verfahren und kits zur nachweis, identifizierung und/oder die zählung von bakterien
US20030072689A1 (en) * 2001-08-15 2003-04-17 Third Wave Technologies, Inc. Polymer synthesizer
US20080261220A1 (en) * 2000-11-30 2008-10-23 Third Wave Technologies, Inc. Nucleic Acid Detection Assays
US6932943B1 (en) 2001-01-26 2005-08-23 Third Wave Technologies Nucleic acid synthesizers
US7435390B2 (en) * 2001-01-26 2008-10-14 Third Wave Technologies, Inc. Nucleic acid synthesizers
WO2002048385A2 (en) 2000-12-12 2002-06-20 Invitrogen Corporation Compositions and methods for the release of nucleic acid molecules from solid matrices
DE60207995T2 (de) * 2001-03-08 2006-08-03 Applera Corp., Foster City Verfahren zum entschützen von oligonukleotiden
AU2002353001A1 (en) * 2001-12-03 2003-06-17 The Government Of The United States Of America, Represented By The Secretary Of The Department Of He Thermolabile hydroxyl protecting groups and methods of use
WO2004022578A2 (en) * 2002-09-08 2004-03-18 Applera Corporation Methods, compositions and libraries pertaining pna dimer and pna oligomer synthesis
US9809824B2 (en) * 2004-12-13 2017-11-07 The United States Of America, Represented By The Secretary, Department Of Health And Human Services CpG oligonucleotide prodrugs, compositions thereof and associated therapeutic methods
US7608433B2 (en) 2005-02-09 2009-10-27 Idexx Laboratories Method of detection of classical swine fever
EP1888780A4 (de) * 2005-05-06 2009-11-11 Applera Corp Mehrfachkapillarenvorrichtung sowie verfahren zur synthese und abgabe
KR101426530B1 (ko) * 2012-10-04 2014-08-05 한국과학기술연구원 형광 검출기를 포함한 모세관 전기영동법을 이용한 ｍｉＲＮＡ의 검출 방법
EP3805242A1 (de) * 2019-10-07 2021-04-14 Sterna Biologicals GmbH & Co. KG Verfahren zur herstellung eines katalytisch aktiven dna-moleküls mit verbesserter aktivität und dessen verwendung in einem verfahren zur behandlung von asthma
CN111704644B (zh) * 2020-08-18 2020-12-04 苏州金唯智生物科技有限公司 一种氨解液及氨解方法
CN114671921A (zh) * 2022-05-06 2022-06-28 南京吉盛澳玛生物医药有限公司 一种大规模寡聚核苷酸氨解方法
CN115850350A (zh) * 2022-12-22 2023-03-28 苏州泓迅生物科技股份有限公司 一种氨解液及氨解方法

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US4033977A (en) * 1975-10-01 1977-07-05 American Home Products Corporation 4H-pyrano[4,3-d]thiazole derivatives and process therefor
US4242097A (en) * 1979-03-05 1980-12-30 Dionex Corporation Method and apparatus for quantitative analysis of weakly ionized anions or cations
DE2940709A1 (de) * 1979-10-08 1981-04-16 Basf Ag, 6700 Ludwigshafen Verfahren zur herstellung von in 1-stellung substituierten imidazolen
US4500707A (en) * 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4973679A (en) * 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
CA1179954A (en) * 1981-08-24 1984-12-27 Hansen M. Hsiung Process for de-cyanoethylating blocked nucleotides
US4507433A (en) * 1983-10-07 1985-03-26 The Johns Hopkins University Preparation of oligodeoxyribonucleoside alkyl or arylphosphonates
US4672110A (en) * 1983-11-29 1987-06-09 Northwestern University Method of deprotecting polynucleotide trimethylethyl phosphotriesters
US4680295A (en) * 1986-02-03 1987-07-14 G. D. Searle & Co. Octahydropyrido[1,2-c]pyrimidinones and hexahydropyrido[1,2-c]pyrimidinediones
FR2596761B1 (fr) * 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
US4908479A (en) * 1987-10-30 1990-03-13 Osaka Yuki Kagaku Kogyo Kabushiki Kaisha N-phenyl-2,2,6,6-tetrahalocyclohexaneimine
US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
GB9204903D0 (en) * 1992-03-06 1992-04-22 Ici Plc Compounds

Also Published As

Publication number Publication date
JP3368353B2 (ja) 2003-01-20
EP0639200A1 (de) 1995-02-22
US5348868A (en) 1994-09-20
JPH07505904A (ja) 1995-06-29
EP0639200B1 (de) 1998-01-07
DE69316205D1 (de) 1998-02-12
WO1993022329A1 (en) 1993-11-11
US5518651A (en) 1996-05-21

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