DE69225120T2 - Verfahren zum Lysieren von Mykobakterien - Google Patents

Verfahren zum Lysieren von Mykobakterien

Info

Publication number
DE69225120T2
DE69225120T2 DE69225120T DE69225120T DE69225120T2 DE 69225120 T2 DE69225120 T2 DE 69225120T2 DE 69225120 T DE69225120 T DE 69225120T DE 69225120 T DE69225120 T DE 69225120T DE 69225120 T2 DE69225120 T2 DE 69225120T2
Authority
DE
Germany
Prior art keywords
lysing mycobacteria
mycobacteria
lysing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69225120T
Other languages
English (en)
Other versions
DE69225120D1 (de
Inventor
Jillian Alyson Robson
Adriann Jeanelle Howard
William Edward Keating
James Arthur Down
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of DE69225120D1 publication Critical patent/DE69225120D1/de
Application granted granted Critical
Publication of DE69225120T2 publication Critical patent/DE69225120T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69225120T 1991-12-18 1992-11-25 Verfahren zum Lysieren von Mykobakterien Expired - Fee Related DE69225120T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US80980691A 1991-12-18 1991-12-18

Publications (2)

Publication Number Publication Date
DE69225120D1 DE69225120D1 (de) 1998-05-20
DE69225120T2 true DE69225120T2 (de) 1998-09-17

Family

ID=25202269

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69225120T Expired - Fee Related DE69225120T2 (de) 1991-12-18 1992-11-25 Verfahren zum Lysieren von Mykobakterien

Country Status (6)

Country Link
US (1) US5376527A (de)
EP (1) EP0547789B1 (de)
JP (1) JP2625340B2 (de)
AU (1) AU664050B2 (de)
CA (1) CA2084533C (de)
DE (1) DE69225120T2 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512440A (en) * 1991-12-18 1996-04-30 Becton Dickinson And Company Process for lysing Mycobacteria
CA2121659C (en) * 1993-05-11 2000-11-28 Michael C. Little Sample processing method for mycobacteria
IL110463A0 (en) * 1993-08-13 1994-10-21 Du Pont In situ extraction of microbial DNA
AU682538B2 (en) * 1993-11-16 1997-10-09 Becton Dickinson & Company Process for lysing mycobacteria
JP3866762B2 (ja) * 1993-11-29 2007-01-10 ジェン−プローブ・インコーポレイテッド 広範な生物からの核酸抽出法
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
US5712095A (en) * 1994-06-16 1998-01-27 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
WO1996000228A1 (en) * 1994-06-23 1996-01-04 Dade International Inc. Method for the rapid isolation of nucleic acid
US5538870A (en) * 1994-09-21 1996-07-23 Boehringer Mannheim Corporation Method for preparing nucleic acids for analysis and kits useful therefor
US5707860A (en) * 1996-03-12 1998-01-13 Becton Dickinson And Company Vehicle for delivery of particles to a sample
US5942425A (en) * 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US6939696B1 (en) * 1998-08-03 2005-09-06 Becton, Dickinson And Company Cell disruption method using sonication
US7494771B2 (en) * 2001-07-19 2009-02-24 Geneohm Sciences Canada, Inc. Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection
WO2003097816A1 (fr) * 2002-05-21 2003-11-27 Arkray, Inc. Procede permettant d'effectuer la lyse d'une bacterie resistante aux acides et procede permettant de realiser une amplification ou une detection de gene
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
CN1856579A (zh) * 2003-09-23 2006-11-01 密苏里大学 使用热稳定酶合成多核苷酸的方法
JP2009532512A (ja) 2006-02-28 2009-09-10 セルラー・バイオエンジニアリング・インコーポレイテッド 重合体組成物、ならびに汚染物を基板から除去するための方法
CA2652454C (en) * 2006-05-24 2013-04-30 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample
DE602007008728D1 (de) * 2006-06-19 2010-10-07 Becton Dickinson Co Verfahren und zusammensetzungen für den erhalt ampsgesetzten geweben, zellen oder viren
US20100099149A1 (en) 2006-10-06 2010-04-22 Dna Genotek Inc. Stabilizing compositions and methods for extraction of ribonucleic acid
JP2008167722A (ja) * 2007-01-15 2008-07-24 Konica Minolta Medical & Graphic Inc 磁性支持体上での加熱による核酸単離方法
US20080317702A1 (en) * 2007-06-19 2008-12-25 Garry Edgington Method for treating microorganisms and/or infectious agents
EP2162498B1 (de) * 2007-06-19 2011-04-13 Cellular Bioengineering, Inc. Verfahren zum schutz von substraten und zur entfernung von verunreinigungen aus solchen substraten
US8158405B2 (en) * 2008-06-30 2012-04-17 General Electric Company Process for concentrating and processing fluid samples
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
EP2210936A1 (de) * 2009-01-27 2010-07-28 Curetis AG Verarbeitung und Analyse von viskosen, flüssigen biologischen Proben
JP5434199B2 (ja) * 2009-03-31 2014-03-05 東洋紡株式会社 抗酸菌遺伝子の増幅方法
JP5916610B2 (ja) 2009-09-03 2016-05-11 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 直接的な化学的溶解のための方法および組成物
WO2011158815A1 (ja) * 2010-06-18 2011-12-22 東洋紡績株式会社 抗酸菌の溶菌とその核酸の分離を同時に行う方法
EP3150702B1 (de) 2011-06-19 2021-05-19 DNA Genotek, Inc. Vorrichtungen, lösungen und verfahren zur probenentnahme
WO2013023021A1 (en) 2011-08-11 2013-02-14 Cellular Bioengineering, Inc. Polymer composition

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5143736B2 (de) * 1971-10-06 1976-11-24
EP0041650A3 (de) * 1980-06-10 1982-05-12 Provesta Corporation Verfahren zur Verminderung des Nucleinsäuregehaltes in einzelligem Protein und Verfahren zur Herstellung eines einzelligen Proteinprodukts
ZA832976B (en) * 1982-05-05 1984-01-25 Phillips Petroleum Co A process for producing physiologically active protein
DD240909A1 (de) * 1983-12-09 1986-11-19 Adw Ddr Verfahren zur herstellung von mykobakterien-biomasse-lysat
CA1296604C (en) * 1986-03-20 1992-03-03 Kathleen Murphy Method for releasing rna and dna from cells
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
FR2651505B1 (fr) * 1989-09-06 1994-07-22 Pasteur Institut Fragments d'acides nucleiques derives d'un genome de mycobacterie appropriee, leurs applications dans le diagnostic des infections a mycobacteries et plasmides contenant lesdits fragments.
EP0490951B1 (de) * 1989-09-06 1996-04-24 Institut Pasteur Nukleinsäurefragmente von einem genom eines geeigneten mykobakteriums, ihre verwendung zur diagnose von mykobakterieninfektionen sowie diese fragmente enthaltende plasmide
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

Also Published As

Publication number Publication date
DE69225120D1 (de) 1998-05-20
EP0547789B1 (de) 1998-04-15
JPH06319527A (ja) 1994-11-22
AU664050B2 (en) 1995-11-02
JP2625340B2 (ja) 1997-07-02
EP0547789A1 (de) 1993-06-23
AU2844892A (en) 1993-06-24
CA2084533C (en) 1998-12-29
US5376527A (en) 1994-12-27
CA2084533A1 (en) 1993-06-19

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee